BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,739,094	T→C	13.7%	P110P (CCA→CCG)	hutU ←	urocanate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,739,094	0	T	C	13.7%	931.6 / 107.0	351	P110P (CCA→CCG)	hutU	urocanate hydratase
Reads supporting (aligned to +/- strand): ref base T (170/133); new base C (25/23); total (195/156)
Fisher's exact test for biased strand distribution p-value = 6.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 357 total aligned reads displayed.

TGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGT  >  NC_002947/5738945‑5739242

tGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAgtgt                                                                                                                                                      <  1:2143544/150‑1 (MQ=255)

 gCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGtgtg                                                                                                                                                     <  1:799805/150‑1 (MQ=255)

  cTGGCGACCGGCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCgg                                                                                                                                                    <  2:1014795/150‑1 (MQ=255)

        aCCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAg                                                                                                                                              >  2:5284027/1‑150 (MQ=255)

          cGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGt                                                                                                                                            <  2:3335332/150‑1 (MQ=255)

            gCTTCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTg                                                                                                                                          <  2:8388782/150‑1 (MQ=255)

              ttCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGa                                                                                                                                        >  1:3013242/1‑150 (MQ=255)

              ttCCACGAAGGTTTCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGa                                                                                                                                        >  2:5216039/1‑150 (MQ=255)

                 gacgaAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATTGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGtt                                                                                                                                     >  2:4296407/1‑150 (MQ=255)

                 gacgaAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGtt                                                                                                                                     >  2:6600480/1‑150 (MQ=255)

                 gacgaAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGtt                                                                                                                                     <  2:713790/150‑1 (MQ=255)

                 gacgaAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGtt                                                                                                                                     >  2:7975206/1‑150 (MQ=255)

                  acgaAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTg                                                                                                                                    <  1:8878192/150‑1 (MQ=255)

                  acgaAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTg                                                                                                                                    >  2:6348244/1‑150 (MQ=255)

                    gaAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGc                                                                                                                                  >  2:775026/1‑150 (MQ=255)

                    gaAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGc                                                                                                                                  >  1:1753748/1‑150 (MQ=255)

                         tttCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCa                                                                                                                             <  1:9114241/150‑1 (MQ=255)

                         tttCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCa                                                                                                                             <  1:4198478/150‑1 (MQ=255)

                         tttCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCa                                                                                                                             <  1:4810025/150‑1 (MQ=255)

                         tttCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGAtga                                                                                                                             >  1:2502316/1‑148 (MQ=255)

                         tttCATAGGTGCCCTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGAtga                                                                                                                             <  2:448412/150‑3 (MQ=255)

                             aTAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCAc                                                                                                                         >  1:1018377/1‑150 (MQ=255)

                                    cccTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGt                                                                                                                  <  1:2963307/150‑1 (MQ=255)

                                    cccTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGt                                                                                                                  >  1:3933214/1‑150 (MQ=255)

                                    cccTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGt                                                                                                                  >  1:1874383/1‑150 (MQ=255)

                                    cccTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGATGAGAACGCGCGGt                                                                                                                  <  2:400734/150‑1 (MQ=255)

                                    cccTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGATGAGAACGCGCGGt                                                                                                                  >  2:1495742/1‑150 (MQ=255)

                                    cccTGAACAATGCCCTGTGCACCGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGATGAGAACGCGCGGt                                                                                                                  <  2:914794/150‑1 (MQ=255)

                                     ccTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTg                                                                                                                 >  2:5863143/1‑150 (MQ=255)

                                     ccTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTg                                                                                                                 >  2:6018593/1‑150 (MQ=255)

                                      cTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGc                                                                                                                >  1:2822158/1‑150 (MQ=255)

                                       tGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCg                                                                                                               >  1:2233648/1‑150 (MQ=255)

                                         gACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGtt                                                                                                             >  1:5447980/1‑150 (MQ=255)

                                              tGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCtgt                                                                                                        <  2:3862347/150‑1 (MQ=255)

                                              tGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCtgt                                                                                                        <  2:8672404/150‑1 (MQ=255)

                                               gCCCTGGCTGCCGATGTAGCTCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCtgtg                                                                                                       <  1:6831713/150‑1 (MQ=255)

                                               gCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCtgtg                                                                                                       >  1:3039362/1‑150 (MQ=255)

                                                cccTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTgg                                                                                                      <  2:1287454/150‑1 (MQ=255)

                                                cccTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTgg                                                                                                      <  1:7427484/150‑1 (MQ=255)

                                                 ccTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTggg                                                                                                     <  2:5754634/150‑1 (MQ=255)

                                                      cTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTg                                                                                                >  1:2330489/1‑150 (MQ=255)

                                                        gCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGaa                                                                                              <  1:4547579/150‑1 (MQ=255)

                                                        gCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGaa                                                                                              <  1:5050390/150‑1 (MQ=255)

                                                        gCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGaa                                                                                              <  2:3428039/150‑1 (MQ=255)

                                                        gCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGaa                                                                                              >  1:5877045/1‑150 (MQ=255)

                                                        accGATGTAAATCCACGAACCGGCGGTCATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGATGAGAACGCGCGGTGCATCCTTGTGGGtgcggaa                                                                                              >  2:1741080/2‑144 (MQ=255)

                                                          cGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAaca                                                                                            <  1:1541597/150‑1 (MQ=255)

                                                           gATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTgg                                                                                                                                                    >  2:5499955/1‑93 (MQ=255)

                                                            aTGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAacaca                                                                                          <  1:8640744/150‑1 (MQ=255)

                                                                 gATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACaccgac                                                                                     >  2:2394436/1‑150 (MQ=255)

                                                                   tCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACaccgaccg                                                                                   >  1:77712/1‑150 (MQ=255)

                                                                      aGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCt                                                                                >  1:4509742/1‑150 (MQ=255)

                                                                           ccGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGccc                                                                           >  2:8177835/1‑150 (MQ=255)

                                                                           ccGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGccc                                                                           <  1:3394510/150‑1 (MQ=255)

                                                                             ggcggTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGa                                                                         <  2:1782366/150‑1 (MQ=255)

                                                                                ggTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTg                                                                      <  1:6425485/150‑1 (MQ=255)

                                                                                   cATCTGGCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGATGAGAACGCGCGGTGCATCCTTGTGGGTGCGGAACACGCCCACCGGCTTGCCCGACTGCAc                                                                   <  1:6171804/150‑1 (MQ=255)

                                                                                    aTCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCAcc                                                                  >  2:2914468/1‑150 (MQ=255)

                                                                                    aTCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCAcc                                                                  >  2:6829248/1‑150 (MQ=255)

                                                                                     tCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCTGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACca                                                                 <  1:7944411/150‑1 (MQ=255)

                                                                                     ccTGGCGATGTATGGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGtt                                                                                                                                           <  2:6481088/75‑1 (MQ=255)

                                                                                         tccATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACcagcag                                                             >  2:3098924/2‑150 (MQ=255)

                                                                                         gCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGATGAGAACGCGCGGTGCATCCTTGTGGGTGCGGAACACGCCCACCGGCTTGCCCGACTGCACcagcaa                                                             >  1:7572815/1‑149 (MQ=255)

                                                                                         gCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGATGAGAACGCGCGGTGCATCCTTGTGGGTGCGGAACACGCCCACCGGCTTGCCCGACTGCACcagcaa                                                             >  2:5339602/1‑149 (MQ=255)

                                                                                         gCCATACATGGCGAGACCCTTCTTATCCAGCTCGTTGAAATGATCCCAGGTCGCCCAATGCGGCACGAGATTGGAATTGGCGATGAGAACGCGCGGTGCATCCTTGTGGGTGCGGAACACGCCCACCGGCTTGCCCGACTGCACcagcaa                                                             >  1:5168132/1‑149 (MQ=255)

                                                                                          ccATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAgg                                                            <  2:8717010/150‑1 (MQ=255)

                                                                                          ccATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAgg                                                            <  2:5041108/150‑1 (MQ=255)

                                                                                             tACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGtt                                                         <  2:765978/150‑1 (MQ=255)

                                                                                              aCATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGttt                                                        >  1:316506/1‑150 (MQ=255)

                                                                                               cATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTtc                                                       <  1:2407867/150‑1 (MQ=255)

                                                                                                 tCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTtcgt                                                     >  2:5695434/1‑150 (MQ=255)

                                                                                                 tCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGAGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTtcgt                                                     >  2:6763500/1‑150 (MQ=255)

                                                                                                  cGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTtcgtc                                                    <  2:7078095/150‑1 (MQ=255)

                                                                                                                 gTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGt                                     >  1:3669992/1‑150 (MQ=255)

                                                                                                                 gTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGt                                     <  1:5284027/150‑1 (MQ=255)

                                                                                                                      gTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAggg                                >  1:7310282/1‑150 (MQ=255)

                                                                                                                       ttCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGt                               >  1:2016538/1‑150 (MQ=255)

                                                                                                                           ttGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTcg                           >  2:8909770/1‑150 (MQ=255)

                                                                                                                            tGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTcga                          <  2:4771129/150‑1 (MQ=255)

                                                                                                                            tGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTcga                          >  2:3671997/1‑150 (MQ=255)

                                                                                                                            tGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTcga                          <  2:1579236/150‑1 (MQ=255)

                                                                                                                            tGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTcga                          >  1:8207162/1‑150 (MQ=255)

                                                                                                                                gtgtTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGAt                      >  1:7850185/1‑150 (MQ=255)

                                                                                                                                 tgtTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATc                     >  1:1356085/1‑150 (MQ=255)

                                                                                                                                   ttCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCtt                   >  2:4198581/1‑150 (MQ=255)

                                                                                                                                   ttCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCtt                   >  1:8178981/1‑150 (MQ=255)

                                                                                                                                   ttCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCtt                   >  1:4766991/1‑150 (MQ=255)

                                                                                                                                      ccAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTc                >  2:2457749/1‑150 (MQ=255)

                                                                                                                                      ccAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTc                >  2:1883922/1‑150 (MQ=255)

                                                                                                                                      ccAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTc                >  1:669668/1‑150 (MQ=255)

                                                                                                                                       cAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCg               >  2:4658415/1‑150 (MQ=255)

                                                                                                                                         gTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTa             >  1:4695669/1‑150 (MQ=255)

                                                                                                                                          ttGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCCAAGGTCATGtagtag            >  2:2666040/1‑150 (MQ=255)

                                                                                                                                            ggCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCa          >  1:5090431/1‑150 (MQ=255)

                                                                                                                                             gCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAg         >  2:9047960/1‑150 (MQ=255)

                                                                                                                                              cccAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGGTTCGACGATCTGGTCGTCGCAgg        >  2:8513345/1‑150 (MQ=255)

                                                                                                                                               ccAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGc       >  2:7386896/1‑150 (MQ=255)

                                                                                                                                                cAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGcc      <  2:2286705/150‑1 (MQ=255)

                                                                                                                                                 aGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGccc     <  2:1375345/150‑1 (MQ=255)

                                                                                                                                                  gtgtGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCa    <  1:5230450/150‑1 (MQ=255)

TGCTGGCGACCGGCTTCGACGAAGGTTTCATAGGTGCCCTGGACGATGCCCTGGCTGCCGATGTAGATCCAGCTGCCGGCGGTCATCTGGCCATACATCGCCAGGCCCTTGGCGTCCAGTTCGTTGAAGTGTTCCCAGTTGGCCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGT  >  NC_002947/5738945‑5739242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`