BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	433,898	A→G	5.2%	R435R (CGT→CGC)	glcB ←	malate synthase G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	433,898	0	A	G	5.2%	1178.5 / 31.6	364	R435R (CGT→CGC)	glcB	malate synthase G
Reads supporting (aligned to +/- strand): ref base A (174/170); new base G (10/9); total (184/180)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01

Only 100 of 366 total aligned reads displayed.

TTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTGCATCTTCGGCTTGACGATGTACACGCTGCCGCTGCGGGT  >  NC_002947/433749‑434047

   tcGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGACTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACAACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACgc                                                                                                                                                    >  1:8661613/1‑149 (MQ=255)

    cGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGc                                                                                                                                                   <  2:2787741/150‑1 (MQ=255)

     gTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCg                                                                                                                                                  >  2:8705756/1‑150 (MQ=255)

     gTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCg                                                                                                                                                  >  1:8629740/1‑150 (MQ=255)

     gTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCg                                                                                                                                                  >  2:3026889/1‑150 (MQ=255)

      ttcttcATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGt                                                                                                                                                 >  1:240089/1‑150 (MQ=255)

      ttcttcATGGCACCTTTGCGCACCACGGCGCCGGCTACCATCGAGGTGTGGATTTCATCGCCAGTGAGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGt                                                                                                                                                 >  2:7082084/1‑150 (MQ=255)

        cttcATGTCGCCCTTGCGGATCATCGGGCCAGCTTCCATCGAGGTATGGATTTCGTCGCCAGTGCGGTCAAGGAAGCCGGTATTGATGAAGACGACGCGTTCCTTGGCAGCGCGGATCGCTTCCTTGAGGTTAACCGTGGTGCGGCGTTc                                                                                                                                               <  1:3535803/150‑1 (MQ=255)

        cttcATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTc                                                                                                                                               <  2:8321806/150‑1 (MQ=255)

         ttcATGTCGCCCTTGCGGATCATCGGGCCAGCTTCCATCGAGGTATGGATTTCGTCGCCAGTGCGGTCAAGGAAGCCGGTATTGATGAAGACGACGCGTTCCTTGGCAGCGCGGATCGCTTCCTTGAGGTTAACCGTGGTGCGGCGTTcc                                                                                                                                              <  1:2853113/150‑1 (MQ=255)

         ttcATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTcc                                                                                                                                              >  2:4410941/1‑150 (MQ=255)

               gCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTcc                                                                                                                                        <  1:1387432/150‑1 (MQ=255)

                cACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCa                                                                                                                                       <  2:2625240/150‑1 (MQ=255)

                cACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCa                                                                                                                                       >  1:5664158/1‑150 (MQ=255)

                          caccacGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGAc                                                                                                                             <  2:8233919/150‑1 (MQ=255)

                           accacGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGAcc                                                                                                                            >  1:6078876/1‑150 (MQ=255)

                           accacGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGAcc                                                                                                                            >  1:2067177/1‑150 (MQ=255)

                             cacGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCtt                                                                                                                          >  2:318319/1‑150 (MQ=255)

                                ggCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGAc                                                                                                                       <  2:4847103/150‑1 (MQ=255)

                                  cgcCGGCTTCCTTCGAGGTGTGGATTTCATCGCCAGTGCTGTAAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCTTTCCTCGTCCATGATGCCGACCTTGACgg                                                                                                                     <  2:6978572/150‑1 (MQ=255)

                                  cgcCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACgg                                                                                                                     <  2:2254973/150‑1 (MQ=255)

                                      ggCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGtt                                                                                                                 >  1:1736165/1‑150 (MQ=255)

                                           ccATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGcgcg                                                                                                            >  1:4126752/1‑150 (MQ=255)

                                             aTCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGc                                                                                                          >  1:7087053/1‑150 (MQ=255)

                                              tCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCa                                                                                                         >  2:4958393/1‑150 (MQ=255)

                                              tCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCa                                                                                                         >  1:4428993/1‑150 (MQ=255)

                                               cGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCAt                                                                                                        <  2:7133054/150‑1 (MQ=255)

                                                gAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATg                                                                                                       <  2:5490079/150‑1 (MQ=255)

                                                  ggTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGcc                                                                                                     <  1:3926016/150‑1 (MQ=255)

                                                    tgtgGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCca                                                                                                   >  2:9033215/1‑150 (MQ=255)

                                                      tgGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCcagc                                                                                                 >  1:8738577/1‑150 (MQ=255)

                                                      tgGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCcagc                                                                                                 <  1:5917872/150‑1 (MQ=255)

                                                       ggATTTCGTCGCCAGTGCGGTCAAGGAAGCCGGTATTGATGAAGACGACGCGTTCCTTGGCAGCGCGGATCGCTTCCTTGAGGTTAACCGTGGTGCGGCGTTCCTCATCCATGATGCCGATTTTCAGTGTGTTGGGCTTCATACCaagca                                                                                                >  1:2313829/1‑150 (MQ=255)

                                                       ggATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCcagca                                                                                                <  1:9046908/150‑1 (MQ=255)

                                                       ggATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCcagca                                                                                                <  1:5221459/150‑1 (MQ=255)

                                                       ggATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCAGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCcagca                                                                                                <  1:3822010/150‑1 (MQ=255)

                                                         aTTTCGTCGCCAGTGCGGTCAAGGAAGCCGGTATTGATGAAGACGACGCGTTCCTTGGCAGCGCGGATCGCTTCCTTGAGGTTAACCGTGGTGCGGCGTTCCTCATCCATGATGCCGATTTTCAGTGTGTTGGGCTTCATACCaagcagc                                                                                              >  2:1560990/1‑149 (MQ=255)

                                                         aTTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAgg                                                                                              >  2:3791880/1‑150 (MQ=255)

                                                            tCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCt                                                                                           >  2:5304276/1‑150 (MQ=255)

                                                              aTCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTc                                                                                         <  1:425715/150‑1 (MQ=255)

                                                               tCGCCAGTGCGGTCAAGGAAGCCGGTATTGATGAAGACGACGCGTTCCTTGGCAGCGCGGATCGCTTCCTTGAGGTTAACCGTGGTGCGGCGTTCCTCATCCATGATGCCGATTTTCAGTGTGTTGGGCTTCATACCAAGCAGCTCTTCg                                                                                        <  1:2344588/150‑1 (MQ=255)

                                                                 gCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGAc                                                                                      >  2:4128822/1‑150 (MQ=255)

                                                                 gCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTGGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGGGTTGCGCGGCATGCCCAGCAGGTCTTCGAc                                                                                      >  2:2001017/1‑150 (MQ=255)

                                                                  ccAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGAcc                                                                                     >  1:5393032/1‑150 (MQ=255)

                                                                   cAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCt                                                                                    <  2:3358335/150‑1 (MQ=255)

                                                                    aGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTg                                                                                   <  1:1250613/150‑1 (MQ=255)

                                                                     gTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGATGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTgg                                                                                  <  1:6790293/150‑1 (MQ=255)

                                                                     gTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTgg                                                                                  <  2:6076248/150‑1 (MQ=255)

                                                                     gTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTgg                                                                                  <  1:5377337/150‑1 (MQ=255)

                                                                       gCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCt                                                                                <  1:4535242/150‑1 (MQ=255)

                                                                         ggTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGa                                                                              >  2:6308289/1‑150 (MQ=255)

                                                                          gTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGaa                                                                             >  1:845785/1‑150 (MQ=255)

                                                                           tCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAg                                                                            >  2:368873/1‑150 (MQ=255)

                                                                              aGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGAtc                                                                         >  1:2520769/1‑150 (MQ=255)

                                                                               ggAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGAtct                                                                        >  2:6600664/1‑150 (MQ=255)

                                                                                 aaGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGAtcgcc                                                                      <  2:2899112/150‑4 (MQ=255)

                                                                                 aaGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCg                                                                      <  1:97369/150‑1 (MQ=255)

                                                                                    ccGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTg                                                                   <  2:5157206/150‑1 (MQ=255)

                                                                                    ccGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTg                                                                   <  2:8468307/150‑1 (MQ=255)

                                                                                     cGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTgg                                                                  >  1:4828975/1‑150 (MQ=255)

                                                                                     cGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTgg                                                                  >  1:8217459/1‑150 (MQ=255)

                                                                                        tgtTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGa                                                               <  2:2117246/150‑1 (MQ=255)

                                                                                         gtTGATGACCACCCCGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGaa                                                              <  1:5517433/150‑1 (MQ=255)

                                                                                          ttGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAg                                                             <  2:5697523/150‑1 (MQ=255)

                                                                                            gatgaACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGc                                                           <  1:3004016/150‑1 (MQ=255)

                                                                                            gatgaACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGc                                                           >  2:7891102/1‑150 (MQ=255)

                                                                                             atgaACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGACGATCTCGGTGGCGAAGGcc                                                          <  2:6677702/150‑1 (MQ=255)

                                                                                             atgaACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGcc                                                          >  1:156135/1‑150 (MQ=255)

                                                                                             atgaACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGcc                                                          >  1:7700592/1‑150 (MQ=255)

                                                                                               gaACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCAc                                                        >  1:6645504/1‑150 (MQ=255)

                                                                                                aaCACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACt                                                       >  1:3131189/1‑150 (MQ=255)

                                                                                                   accacGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTcc                                                    >  2:5404281/1‑150 (MQ=255)

                                                                                                     cacGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTc                                                  >  1:6430849/1‑150 (MQ=255)

                                                                                                      acGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCa                                                 <  2:1931076/150‑1 (MQ=255)

                                                                                                      acGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCa                                                 <  2:560824/150‑1 (MQ=255)

                                                                                                      acGCGCTCGGCGGCTGACTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCTTGATGCAGACCTTGAGGGTGTTGCGCGGCATGCCCAGCAGGTATTGGACCTTGCTGAAGATCTCGGTGGCGAAGGACACTTCCTCa                                                 <  1:8892277/150‑1 (MQ=255)

                                                                                                            tCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCg                                           >  2:2500433/1‑150 (MQ=255)

                                                                                                             cggcggCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGt                                          <  1:5039687/150‑1 (MQ=255)

                                                                                                             cggcggCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGt                                          <  1:3308694/150‑1 (MQ=255)

                                                                                                                cggcTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTGCa                                       >  2:1864785/1‑150 (MQ=255)

                                                                                                                  gcTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGc                                                           >  2:876610/1‑128 (MQ=255)

                                                                                                                    tGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTGCATCtt                                   >  2:7224979/1‑150 (MQ=255)

                                                                                                                     gCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTGCATCTTc                                  >  1:7358732/1‑150 (MQ=255)

                                                                                                                       cTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTGCATCTTCgg                                >  1:7176192/1‑150 (MQ=255)

                                                                                                                       cTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTGCATCTTCgg                                >  2:5935134/1‑150 (MQ=255)

                                                                                                                       cTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTGCATCTTCgg                                <  2:6452116/150‑1 (MQ=255)

                                                                                                                        ttGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTGCATCTTCGGc                               >  2:7509475/1‑150 (MQ=255)

                                                                                                                        ttGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTGCATCTTCGGc                               >  1:1103161/1‑150 (MQ=255)

                                                                                                                        ttGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTGCATCTTCGGc                               >  2:7543122/1‑150 (MQ=255)

                                                                                                                            tGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTGCATCTTCGGCTTGa                           <  1:1995313/150‑1 (MQ=255)

                                                                                                                                  aCTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTGCATCTTCGGCTTGACGATGt                     <  1:4958393/150‑1 (MQ=255)

                                                                                                                                      gAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTGCATCTTCGGCTTGACGATGTacac                 <  2:1234861/150‑1 (MQ=255)

                                                                                                                                         gTTGACCGTGGTACGGCGTTCGTCGTCTATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGTTGTCGGTGGCGAAGGCCACTTCCTCAGGGCGGTGCATCTTCGGCTTGTCGTTGTACAcgct              >  2:4613991/1‑150 (MQ=255)

                                                                                                                                         gTTGACCGTGGTACGGCGTTCCTCGTCTATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTGCATCTTCGGCTTGACGATGTACAcgct              >  2:6009799/1‑150 (MQ=255)

                                                                                                                                         gTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTGCATCTTCGGCTTGACGATGTACAcgct              >  1:5575454/1‑150 (MQ=255)

                                                                                                                                             aCCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTGCATCTTCGGCTTGACGATGTACAcgctgccg          >  1:7841829/1‑150 (MQ=255)

                                                                                                                                               cGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTGCATCTTCGGCTTGACGATGTACAcgctgccgct        <  2:2136183/150‑1 (MQ=255)

                                                                                                                                                   gTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTGCATCTTCGGCTTGACGATGTACACGCTGCCGCTGCgg    <  2:738642/150‑1 (MQ=255)

                                                                                                                                                   gTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTGCATCTTCGGCTTGACGATGTACACGCTGCCGCTGCgg    <  2:6422879/150‑1 (MQ=255)

                                                                                                                                                   gTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTGCATCTTCGGCTTGACGATGTACACGCTGCCGCTGCgg    <  1:6290050/150‑1 (MQ=255)

TTCTCGTTCTTCATGGCACCTTTGCGCACCACGGCGCCGGCTTCCATCGAGGTGTGGATTTCATCGCCAGTGCGGTCAAGGAAGCCGGTGTTGATGAACACCACGCGCTCGGCGGCTGCCTTGATGCAGGACTTGAGGTTGACCGTGGTACGGCGTTCCTCGTCCATGATGCCGACCTTGACGGTGTTGCGCGGCATGCCCAGCAGGTCTTCGACCTGGCTGAAGATCTCGGTGGCGAAGGCCACTTCCTCAGGGCCGTGCATCTTCGGCTTGACGATGTACACGCTGCCGCTGCGGGT  >  NC_002947/433749‑434047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`