Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,739,236 G→T 6.3% A63D (GCC→GAC) ‡ hutU ← urocanate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,739,2360GT 6.3% 1293.7 / 51.7 391A63D (GCC→GAC) ‡hutUurocanate hydratase
Reads supporting (aligned to +/- strand):  ref base G (202/162);  new base T (15/10);  total (217/174)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 403 total aligned reads displayed.

CCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTGGGGCA  >  NC_002947/5739087‑5739385
                                                                                                                                                     |                                                                                                                                                     
cccAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAgg                                                                                                                                                       >  2:8513239/1‑150 (MQ=255)
cccAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAgg                                                                                                                                                       >  1:3411579/1‑150 (MQ=255)
 ccAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGc                                                                                                                                                      >  2:7386896/1‑150 (MQ=255)
  cAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGcc                                                                                                                                                     <  2:2286705/150‑1 (MQ=255)
      gtgGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGt                                                                                                                                                 >  1:310328/1‑150 (MQ=255)
         gCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTgc                                                                                                                                              >  2:1778432/1‑150 (MQ=255)
           aCCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTgcgc                                                                                                                                            >  2:8713816/1‑150 (MQ=255)
                    gAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGcgcggcgcgg                                                                                                                                   <  1:4690068/150‑1 (MQ=255)
                          gCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATa                                                                                                                             <  1:7057220/150‑1 (MQ=255)
                            gatgagaaCGCGCGGTGCATCCTTGTGGGTGCGGAACACGCCCACCGGCTTGCCCGACTGCACCAGCAAGGTTTCGTCTTCATTGAGCGTCTTGAGGGTCGCGACAATCTTGTCGAAATCATCCCATGTGCGCGCAGCGCGGCCAATGcc                                                                                                                           >  2:7133865/8‑150 (MQ=255)
                            aaTCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATAcc                                                                                                                           >  1:4834120/1‑150 (MQ=255)
                             atgagaaCGCGCGGTGCATCCTTGTGGGTGCGGAACACGCCCACCGGCTTGCCCGACTGCACCAGCAAGGTTTCGTCTTCATTGAGCGTCTTGAGGGTCGCGACAATCTTGTCGAAATCATCCCATGTGCGCGCAGCGCGGCCAATGccg                                                                                                                          <  2:7572815/144‑1 (MQ=255)
                             atgagaaCGCGCGGTGCATCCTTGTGGGTGCGGAACACGCCCACCGGCTTGCCCGACTGCACCAACAAGGTTTCGTCTTCATTGAGCGTCTTGAGGGTCGCGACAATCTTGTCGAAATCATCCCATGTGCGCGCAGCGCGGCCAATGccg                                                                                                                          >  1:5496242/7‑150 (MQ=255)
                                aGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATAccgccg                                                                                                                       <  2:2371729/150‑1 (MQ=255)
                                   aCGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGACCCAGTTGCGCGCGGCGCGGCCGATACCGCCGtat                                                                                                                    <  2:6353355/150‑1 (MQ=255)
                                         ggTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATaccacc                                                                                                              <  1:4296407/150‑1 (MQ=255)
                                         ggTGCGTTGCTGTGGGTCGTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACAGCCGTATaccacc                                                                                                              <  2:3012924/150‑1 (MQ=255)
                                          gTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATAccacca                                                                                                             <  1:770844/150‑1 (MQ=255)
                                                  cTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTc                                                                                                     <  2:510359/150‑1 (MQ=255)
                                                  cTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTc                                                                                                     <  2:2233648/150‑1 (MQ=255)
                                                   tgtgGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCg                                                                                                    >  1:2999144/1‑150 (MQ=255)
                                                    gtgGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCgg                                                                                                   <  1:197474/150‑1 (MQ=255)
                                                     tgGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCggg                                                                                                  >  1:4100804/1‑150 (MQ=255)
                                                         tCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGtttt                                                                                              >  2:5634232/1‑150 (MQ=255)
                                                           ttGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTcc                                                                                            <  1:2660208/150‑1 (MQ=255)
                                                           ttGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTcc                                                                                            >  1:1642237/1‑150 (MQ=255)
                                                             gAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCcgc                                                                                          >  2:8098342/1‑150 (MQ=255)
                                                               acacgcCCACCGGCTTGCCCGACTGCACCAGCAAGGTTTCGTCTTCATTGAGCGTCTTGAGGGTCGCGACAATCTTGTCGAAATCATCCCATGTGCGCGCAGCGCGGCCAATGCCGCCATAGACGACCAGCTCATGCGGGCGTTCGGCGa                                                                                        >  1:4952087/1‑150 (MQ=255)
                                                               acacacCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGa                                                                                        <  2:1018377/150‑1 (MQ=255)
                                                                cacacCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGAc                                                                                       <  2:5253025/150‑1 (MQ=255)
                                                                  cacCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCt                                                                                     <  1:4477079/150‑1 (MQ=255)
                                                                   accgaccgGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCtg                                                                                    >  1:5607939/1‑150 (MQ=255)
                                                                   accgaccgGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCtg                                                                                    >  2:4286111/1‑150 (MQ=255)
                                                                   accgaccgGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCtg                                                                                    >  1:7572144/1‑150 (MQ=255)
                                                                   accgaccgGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCtg                                                                                    >  2:6720666/1‑150 (MQ=255)
                                                                    ccgaccgGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCtgt                                                                                   <  2:3052340/150‑1 (MQ=255)
                                                                     cgaccgGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGt                                                                                                                      <  2:2924023/114‑1 (MQ=255)
                                                                     ccaccgGCTTGCCCGACTGCACCAGCAAGGTTTCGTCTTCATTGAGCGTCTTGAGGGTCGCGACAATCTTGTCGAAATCATCCCATGTGCGCGCAGCGCGGCCAATGCCGCCATAGACGACCAGCTCATGCGGGCGTTCGGCGAcatccg                                                                                  >  2:3243430/3‑145 (MQ=255)
                                                                      gaccgGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTgg                                                                                 >  1:437924/1‑150 (MQ=255)
                                                                         cgGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATc                                                                              >  2:3636613/1‑150 (MQ=255)
                                                                          ggCTTGCCCGACTGCACCAGCAGGGTTTCGTTGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCg                                                                             >  1:3843226/1‑150 (MQ=255)
                                                                          ggCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCg                                                                             <  1:1408204/150‑1 (MQ=255)
                                                                           gCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGAc                                                                                       >  1:1077899/1‑139 (MQ=255)
                                                                             ttGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAgg                                                                          <  2:5877045/150‑1 (MQ=255)
                                                                             ttGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAgg                                                                          >  1:6808195/1‑150 (MQ=255)
                                                                             ttGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAgg                                                                          >  1:8257225/1‑150 (MQ=255)
                                                                                 ccGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGgttgt                                                                      >  2:4697934/1‑150 (MQ=255)
                                                                                   gACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTtc                                                                    <  1:5863143/150‑1 (MQ=255)
                                                                                        cACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAg                                                               >  1:2321409/1‑150 (MQ=255)
                                                                                         aCCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGc                                                              >  1:1740971/1‑150 (MQ=255)
                                                                                             gcagGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATgc                                                          >  1:9133151/1‑150 (MQ=255)
                                                                                              cagGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATgcg                                                         >  2:7991531/1‑150 (MQ=255)
                                                                                                   tttCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTg                                                    <  1:3614179/150‑1 (MQ=255)
                                                                                                   tttCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTg                                                    <  1:5234754/150‑1 (MQ=255)
                                                                                                    ttCGTAGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTgg                                                   <  1:1465469/150‑1 (MQ=255)
                                                                                                     tcgtcgTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGgc                                                  <  1:882008/150‑1 (MQ=255)
                                                                                                      cgtcgtCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGgcg                                                 >  1:7874870/1‑150 (MQ=255)
                                                                                                        tcgtcTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCt                                               >  1:6475227/1‑150 (MQ=255)
                                                                                                          gtcTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTc                                             >  1:8142395/1‑150 (MQ=255)
                                                                                                          gtcTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTc                                             >  1:510866/1‑150 (MQ=255)
                                                                                                          gtcTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTc                                             <  2:77712/150‑1 (MQ=255)
                                                                                                          gtcTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTc                                             >  1:6335923/1‑150 (MQ=255)
                                                                                                           tcTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCg                                            >  2:6967409/1‑150 (MQ=255)
                                                                                                           tcTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCg                                            >  1:7276383/1‑150 (MQ=255)
                                                                                                           tcTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCg                                            >  2:4568827/1‑150 (MQ=255)
                                                                                                             ttCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGt                                          >  1:1299359/1‑150 (MQ=255)
                                                                                                             ttCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGt                                          >  1:4589080/1‑150 (MQ=255)
                                                                                                             ttCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGt                                          >  1:6720386/1‑150 (MQ=255)
                                                                                                               ccAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTca                                        <  1:2394436/150‑1 (MQ=255)
                                                                                                               ccAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTca                                        <  2:5432984/150‑1 (MQ=255)
                                                                                                               ccAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTca                                        <  2:3776200/150‑1 (MQ=255)
                                                                                                                 aGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTcagc                                      <  2:4509742/150‑1 (MQ=255)
                                                                                                                    cGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTcagccag                                   <  1:8093987/150‑1 (MQ=255)
                                                                                                                        tCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCttt                               >  1:3345615/1‑150 (MQ=255)
                                                                                                                          aGGGTATCGACGATCATGTCTTAGCAGGCCCAGATGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCAGAGGATCGAGGATGATCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTATg                             >  2:6871502/1‑150 (MQ=255)
                                                                                                                            ggTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGc                           >  2:9009567/1‑150 (MQ=255)
                                                                                                                            ggTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGc                           >  2:6990073/1‑150 (MQ=255)
                                                                                                                             gTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCg                          <  1:1883922/150‑1 (MQ=255)
                                                                                                                                  gacgaTCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAg                     >  1:3763590/1‑150 (MQ=255)
                                                                                                                                    cgaTCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCt                   <  1:1174953/150‑1 (MQ=255)
                                                                                                                                    cgaTCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCt                   <  1:2902098/150‑1 (MQ=255)
                                                                                                                                     gaTCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCtt                  >  1:6446507/1‑150 (MQ=255)
                                                                                                                                      aTCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCttg                 <  2:4100804/150‑1 (MQ=255)
                                                                                                                                       tCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCttgt                >  1:605264/1‑150 (MQ=255)
                                                                                                                                       tCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCttgt                >  1:6211765/1‑150 (MQ=255)
                                                                                                                                        cTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATCCCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCttgtt               <  2:1356085/150‑1 (MQ=255)
                                                                                                                                        cTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCttgtt               <  2:3967464/150‑1 (MQ=255)
                                                                                                                                        cTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCttgtt               <  2:2062489/150‑1 (MQ=255)
                                                                                                                                        cTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCttgtt               <  2:1675040/150‑1 (MQ=255)
                                                                                                                                        cTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCttgtt               <  2:7310282/150‑1 (MQ=255)
                                                                                                                                        cTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTACATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCttgtt               <  2:8206755/150‑1 (MQ=255)
                                                                                                                                         ttGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCttgttg              <  2:2618232/150‑1 (MQ=255)
                                                                                                                                         ttGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCttgttg              >  2:6206340/1‑150 (MQ=255)
                                                                                                                                           gTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGcc            >  1:8207901/1‑150 (MQ=255)
                                                                                                                                                aGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTg       >  2:1116092/1‑150 (MQ=255)
                                                                                                                                                 gCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTgg      >  2:6147793/1‑150 (MQ=255)
                                                                                                                                                 gCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTgg      <  2:4834120/150‑1 (MQ=255)
                                                                                                                                                  cAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTggg     <  1:8397183/150‑1 (MQ=255)
                                                                                                                                                  cAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTggg     <  1:5750761/150‑1 (MQ=255)
                                                                                                                                                     gCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGACACGTGGGGCa  >  2:1920809/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCCAGTGTGGCACCAGGTTGGAGTTGGCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTGGGGCA  >  NC_002947/5739087‑5739385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑