Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,739,262 A→G 5.4% G54G (GGT→GGC)  hutU ← urocanate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,739,2620AG 5.4% 1329.5 / 37.2 411G54G (GGT→GGC) hutUurocanate hydratase
Reads supporting (aligned to +/- strand):  ref base A (204/183);  new base G (15/7);  total (219/192)
Fisher's exact test for biased strand distribution p-value = 1.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

Only 100 of 421 total aligned reads displayed.

GCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTGGGGCACGGATTTCAACGTCACGGTATTTGTT  >  NC_002947/5739113‑5739411
                                                                                                                                                     |                                                                                                                                                     
gCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATa                                                                                                                                                       <  1:7057220/150‑1 (MQ=255)
gCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATa                                                                                                                                                       >  1:423532/1‑150 (MQ=255)
gCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCCTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATa                                                                                                                                                       >  1:9055272/1‑150 (MQ=255)
  aaTCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATAcc                                                                                                                                                     >  1:4834120/1‑150 (MQ=255)
   atgagaaCGCGCGGTGCATCCTTGTGGGTGCGGAACACGCCCACCGGCTTGCCCGACTGCACCAACAAGGTTTCGTCTTCATTGAGCGTCTTGAGGGTCGCGACAATCTTGTCGAAATCATCCCATGTGCGCGCAGCGCGGCCAATGccg                                                                                                                                                    >  1:5496242/7‑150 (MQ=255)
       gCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGt                                                                                                                                                >  2:4052976/1‑150 (MQ=255)
       gCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGt                                                                                                                                                >  1:4566942/1‑150 (MQ=255)
         aCGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGtat                                                                                                                                              >  2:3386278/1‑150 (MQ=255)
         aCGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGACCCAGTTGCGCGCGGCGCGGCCGATACCGCCGtat                                                                                                                                              <  2:6353355/150‑1 (MQ=255)
          cgcgcgGTGCATCCTTGTGGGTGCGGAACACGCCCACCGGCTTGCCCGACTGCACCAGCAAGGTTTCGTCTTCATTGAGCGTCTTGAGGGTCGCGACAATCTTGTCGAAATCATCCCATGTGCGCGCAGCGCGGCCAATGCCGCCAtaga                                                                                                                                             <  1:7248109/150‑3 (MQ=255)
          cgcgcgGCGCGTCGCTGCGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTTGCAGGCCCAGTTGCGCGCGGCGCTGCCGATACCGCCGtata                                                                                                                                             <  1:8094331/150‑1 (MQ=255)
               ggTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATaccacc                                                                                                                                        <  2:3013242/150‑1 (MQ=255)
                gTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATAccacca                                                                                                                                       <  1:770844/150‑1 (MQ=255)
                  gcatccttgtgGGTGCGGAACACGCCCACTGGCTTGCCCGACTGCACCAGCAAGGTTTCGTCTTCATTGAGCGTCTTGAGGGTCGCGACAATCTTGTCGAAATCATCCCATGTGCGCGCAGCGCGGCCAATGCCGCCATAGACGACCAGc                                                                                                                                     <  2:8200825/143‑1 (MQ=255)
                      ccttgtgGGTGCGGAACACGCCCACCGGCTTGCCCGACTGCACCAGCAAGGTTTCGTCTTCATTGAGCGTCTTGAGGGTCGCGACAATCTTGTCGAAATCATCCCATGTGCGCGCAGCGCGGCCAATGCCGCCATAGACGACCAGctcat                                                                                                                                 <  2:2964733/147‑3 (MQ=255)
                        cTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTc                                                                                                                               >  1:990807/1‑150 (MQ=255)
                                 ttGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTcc                                                                                                                      >  1:307936/1‑150 (MQ=255)
                                 ttGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTcc                                                                                                                      >  1:1642237/1‑150 (MQ=255)
                                 ttGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTcc                                                                                                                      >  1:4361450/1‑150 (MQ=255)
                                 ttGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTcc                                                                                                                      <  1:2660208/150‑1 (MQ=255)
                                   gAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCcgc                                                                                                                    >  2:3772257/1‑150 (MQ=255)
                                     acacgcCCACCGGCTTGCCCGACTGCACCAGCAAGGTTTCGTCTTCATTGAGCGTCTTGAGGGTCGCGACAATCTTGTCGAAATCATCCCATGTGCGCGCAGCGCGGCCAATGCCGCCATAGACGACCAGCTCATGCGGGCGTTCGGCGa                                                                                                                  >  1:4952087/1‑150 (MQ=255)
                                       acgcccaccgGCTTGCCCGACTGCACCAGCAAGGTTTCGTCTTCATTGAGCGTCTTGAGGGTCGCGACAATCTTGTCGAAATCATCCCATGTGCGCGCAGCGCGGCCAATGCCGCCATAGACGACCAGCTCATGCGGGCGTTCGGCGAca                                                                                                                >  1:4455451/7‑149 (MQ=255)
                                       acacCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGAcc                                                                                                                <  1:1288751/150‑1 (MQ=255)
                                         gcccaccgGCTTGCCCGACTGCACCAGCAAGGTTTCGTCTTCATTGAGCGTCTTGAGGGTCGCGACAATCTTGTCGAAATCATCCCATGTGCGCGCAGCGCGGCCAATGCCGCCATAGACGACCAGCTCATGCGGGCGTTCGGCGAcatc                                                                                                              >  2:2370259/5‑147 (MQ=255)
                                         accgaccgGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCtg                                                                                                              >  1:7572144/1‑150 (MQ=255)
                                         accgaccgGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCtg                                                                                                              >  1:5607939/1‑150 (MQ=255)
                                          ccgaccgGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCtgt                                                                                                             <  1:6018593/150‑1 (MQ=255)
                                          ccgaccgGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCtgt                                                                                                             >  1:4677622/1‑150 (MQ=255)
                                           cgaccgGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCtgtg                                                                                                            >  2:2544940/1‑150 (MQ=255)
                                           ccaccgGCTTGCCCGACTGCACCAGCAAGGTTTCGTCTTCATTGAGCGTCTTGAGGGTCGCGACAATCTTGTCGAAATCATCCCATGTGCGCGCAGCGCGGCCAATGCCGCCATAGACGACCAGCTCATGCGGGCGTTCGGCGAcatccg                                                                                                            >  2:3243430/3‑145 (MQ=255)
                                             accgGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGa                                                                                                          >  1:647413/1‑150 (MQ=255)
                                             accgGCTTGCCCGACTGCACCAGCAAGGTTTCGTCTTCATTGAGCGTCTTGAGGGTCGCGACAATCTTGTCGAAATCATCCCATGTGCGCGCAGCGCGGCCAATGCCGCCATAGACGACCAGCTCATGCGGGCGTTCGGCGAcatccggg                                                                                                          >  2:7027566/1‑143 (MQ=255)
                                               cgGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATc                                                                                                        >  2:3636613/1‑150 (MQ=255)
                                                ggCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCg                                                                                                       <  1:1408204/150‑1 (MQ=255)
                                                 gCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGAc                                                                                                                 >  1:1077899/1‑139 (MQ=255)
                                                   ttGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAgg                                                                                                    >  1:6808195/1‑150 (MQ=255)
                                                    tGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGgt                                                                                                   >  1:8177956/1‑150 (MQ=255)
                                                       ccGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGgttgt                                                                                                >  2:136284/1‑150 (MQ=255)
                                                       ccGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGgttgt                                                                                                <  2:8088781/150‑1 (MQ=255)
                                                         gACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTtc                                                                                              <  1:5863143/150‑1 (MQ=255)
                                                         gACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTtc                                                                                              <  2:423532/150‑1 (MQ=255)
                                                           cTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTtcat                                                                                            <  2:4575982/150‑1 (MQ=255)
                                                             gCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTtcatca                                                                                          <  2:7475676/150‑1 (MQ=255)
                                                              cACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAg                                                                                         >  1:2321300/1‑150 (MQ=255)
                                                               aCCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGc                                                                                        >  2:6929654/1‑150 (MQ=255)
                                                               aCCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGc                                                                                        >  1:1740947/1‑150 (MQ=255)
                                                                 cagcagGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCAt                                                                                      >  1:6444891/1‑150 (MQ=255)
                                                                  agcagGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATg                                                                                     >  1:4044941/1‑150 (MQ=255)
                                                                  agcagGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATg                                                                                     >  1:1958242/1‑150 (MQ=255)
                                                                  agcagGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATg                                                                                     >  2:6842323/1‑150 (MQ=255)
                                                                         tttCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTg                                                                              <  1:5234561/150‑1 (MQ=255)
                                                                         tttCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTg                                                                              <  1:3614179/150‑1 (MQ=255)
                                                                          ttCGTAGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTgg                                                                             <  1:1465469/150‑1 (MQ=255)
                                                                            cgtcgtCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGgcg                                                                           >  1:7874870/1‑150 (MQ=255)
                                                                                gtcTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTc                                                                       >  1:6335922/1‑150 (MQ=255)
                                                                                   ttCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGt                                                                    >  1:6720386/1‑150 (MQ=255)
                                                                                   ttCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGt                                                                    >  1:2813514/1‑150 (MQ=255)
                                                                                     ccAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTca                                                                  <  1:2394436/150‑1 (MQ=255)
                                                                                       aGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTcagc                                                                <  2:4509760/150‑1 (MQ=255)
                                                                                       aGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTcagc                                                                >  1:8577304/1‑150 (MQ=255)
                                                                                       aGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTcagc                                                                >  2:1699777/1‑150 (MQ=255)
                                                                                       aGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTcagc                                                                <  2:2031638/150‑1 (MQ=255)
                                                                                              tCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCttt                                                         >  1:3345615/1‑150 (MQ=255)
                                                                                                aGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTg                                                       >  1:3409204/1‑150 (MQ=255)
                                                                                                aGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTg                                                       <  2:5079567/150‑1 (MQ=255)
                                                                                                aGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTg                                                       >  2:5470344/1‑150 (MQ=255)
                                                                                                   gTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGgt                                                                                                   >  2:5755195/1‑103 (MQ=255)
                                                                                                   gTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCg                                                    <  2:793559/150‑1 (MQ=255)
                                                                                                   gTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCg                                                    <  2:4790562/150‑1 (MQ=255)
                                                                                                     ttCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGt                                                  <  2:5204336/150‑1 (MQ=255)
                                                                                                        gacgaTCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAg                                               >  1:3763590/1‑150 (MQ=255)
                                                                                                        gacgaTCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTCGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAg                                               >  2:2191450/1‑150 (MQ=255)
                                                                                                         acgaTCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGc                                              <  2:697356/150‑1 (MQ=255)
                                                                                                          cgaTCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCt                                             <  1:1174953/150‑1 (MQ=255)
                                                                                                             tCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCttgt                                          >  2:7295181/1‑150 (MQ=255)
                                                                                                               ttGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCttgttg                                        >  1:8223516/1‑150 (MQ=255)
                                                                                                               ttGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCttgttg                                        >  2:6206340/1‑150 (MQ=255)
                                                                                                                      aGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTg                                 <  2:3071611/150‑1 (MQ=255)
                                                                                                                      aGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTg                                 >  2:1115665/1‑150 (MQ=255)
                                                                                                                      aGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTg                                 <  2:7724925/150‑1 (MQ=255)
                                                                                                                       gCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTgg                                >  2:6147778/1‑150 (MQ=255)
                                                                                                                       gCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTgg                                <  2:5393536/150‑1 (MQ=255)
                                                                                                                        cAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTggg                               <  1:8397183/150‑1 (MQ=255)
                                                                                                                        cAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTggg                               <  1:5750761/150‑1 (MQ=255)
                                                                                                                           gCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGACACGTGGGGCa                            >  2:1920809/1‑150 (MQ=255)
                                                                                                                            cccAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTGGGGCAc                           <  1:7386896/150‑1 (MQ=255)
                                                                                                                             ccAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTGGGGCACg                          >  2:474964/1‑150 (MQ=255)
                                                                                                                             ccAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTGGGGCACg                          >  2:2809188/1‑150 (MQ=255)
                                                                                                                               aGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTGGGGCACGGa                        >  2:8339199/1‑150 (MQ=255)
                                                                                                                                     gcgcggcgcgGCCGATACCGCCGTATACCACCCGCTCTTTCGGGTGTTCCGCGACCTGGGGATCGAGGTTGGTCATCAGCATGCGCAGTGGCGCGTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTGGGGCACGGATTTCaa                  <  2:8998814/150‑1 (MQ=255)
                                                                                                                                     gcgcggcgcgGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTGGGGCACGGATTTCaa                  >  1:6204065/1‑150 (MQ=255)
                                                                                                                                     gcgcggcgcgGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTGGGGCACGGATTTCaa                  <  1:3805327/150‑1 (MQ=255)
                                                                                                                                      cgcggcgcggCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTGGGGCACGGATTTCAAc                 >  2:6048546/1‑150 (MQ=255)
                                                                                                                                      cgcggcgcggCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTGGGGCACGGATTTCAAc                 <  1:6362213/150‑1 (MQ=255)
                                                                                                                                       gcggcgcggcCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTGGGGCACGGATTTCAACg                >  2:1299580/1‑150 (MQ=255)
                                                                                                                                        cggcgcggcCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTGGGGCACGGATTTCAACGt               <  1:1778432/150‑1 (MQ=255)
                                                                                                                                         ggcgcggcCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTGGGGCACGGATTTCAACGTc              >  2:2252844/1‑150 (MQ=255)
                                                                                                                                          gcgcggcCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTGGGGCACGGATTTCAACGTCa             >  1:2982012/1‑150 (MQ=255)
                                                                                                                                                  gATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTGGGGCACGGATTTCAACGTCACGGTAttt     <  2:437924/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCAATCAGCACGCGCGGTGCGTTGCTGTGGGTCTTGAACACACCGACCGGCTTGCCCGACTGCACCAGCAGGGTTTCGTCGTCTTCCAGGCGGGTCAGGGTTTCGACGATCTTGTCGTAGCAGGCCCAGTTGCGCGCGGCGCGGCCGATACCGCCGTATACCACCAGCTCTTTCGGGTTTTCCGCGACCTGTGGATCGAGGTTGTTCATCAGCATGCGCAGTGGCGCTTCGGTCAGCCAGCTTTTGGCGGTCAGCTTGTTGCCACGTGGGGCACGGATTTCAACGTCACGGTATTTGTT  >  NC_002947/5739113‑5739411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑