BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,746,092	C→A	6.4%	W584L (TGG→TTG)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,746,092	0	C	A	6.4%	837.0 / 31.3	249	W584L (TGG→TTG)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand): ref base C (119/114); new base A (11/5); total (130/119)
Fisher's exact test for biased strand distribution p-value = 2.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 257 total aligned reads displayed.

GTTGGGCAGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATCCTGAATG  >  NC_002947/5745944‑5746241

   gggCAGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGtt                                                                                                                                                   <  2:6769898/150‑1 (MQ=255)

   gggCAGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGtt                                                                                                                                                   >  1:149771/1‑150 (MQ=255)

     gCAGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTgg                                                                                                                                                 >  1:6809614/1‑150 (MQ=255)

       aGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACAACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTg                                                                                                                                               >  1:2643256/1‑150 (MQ=255)

        ggAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGc                                                                                                                                              >  2:3658977/1‑150 (MQ=255)

         gAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGcc                                                                                                                                             >  1:6617950/1‑150 (MQ=255)

          aaCACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCa                                                                                                                                            <  2:8334326/150‑1 (MQ=255)

             accaccTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGt                                                                                                                                         >  1:2536441/1‑150 (MQ=255)

             accaccTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGt                                                                                                                                         >  1:2778937/1‑150 (MQ=255)

             accaccTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCAACTGGGTGccgcgg                                                                                                                                         <  1:6505523/150‑5 (MQ=255)

              ccaccTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCAACTGGGTGccgcggg                                                                                                                                        <  1:4378237/150‑6 (MQ=255)

               caccTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTc                                                                                                                                       >  1:7932488/1‑150 (MQ=255)

               caccTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTc                                                                                                                                       <  1:464761/150‑1 (MQ=255)

               caccTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTc                                                                                                                                       >  1:499346/1‑150 (MQ=255)

                  cTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGtt                                                                                                                                    <  2:1846829/150‑1 (MQ=255)

                       gcagGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTa                                                                                                                               >  2:8001118/1‑150 (MQ=255)

                         agGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATg                                                                                                                             <  2:6948057/150‑1 (MQ=255)

                              gcgcACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTg                                                                                                                        <  2:869234/150‑1 (MQ=255)

                                gcACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGt                                                                                                                      <  1:8326156/150‑1 (MQ=255)

                                gcACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGt                                                                                                                      >  2:3420336/1‑150 (MQ=255)

                                gcACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGt                                                                                                                      <  1:1597960/150‑1 (MQ=255)

                                    tGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAg                                                                                                                  >  1:1393919/1‑150 (MQ=255)

                                                gttCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGtgtg                                                                                                      >  1:5497463/1‑150 (MQ=255)

                                                 ttCACCACCCGGGGGATGTCGTTGGAAAGTTTGATGATCAGTTTGGCCTGATGTTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGc                                                                                                     <  1:5843962/150‑1 (MQ=255)

                                                 ttCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGc                                                                                                     >  2:529297/1‑150 (MQ=255)

                                                    accaccCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATc                                                                                                  >  1:3906165/1‑150 (MQ=255)

                                                    accaccCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATc                                                                                                  <  2:3036295/150‑1 (MQ=255)

                                                    accaccCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATc                                                                                                  <  1:172105/150‑1 (MQ=255)

                                                      caccCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAg                                                                                                >  2:6714422/1‑150 (MQ=255)

                                                           gggCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGa                                                                                           <  1:40642/150‑1 (MQ=255)

                                                           gggCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGa                                                                                           <  1:2981211/150‑1 (MQ=255)

                                                           gggCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGa                                                                                           <  2:8620113/150‑1 (MQ=255)

                                                                   tCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCTGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTgg                                                                                   >  2:22861/1‑150 (MQ=255)

                                                                   tCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTgg                                                                                   >  1:3158053/1‑150 (MQ=255)

                                                                   tCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTgg                                                                                   <  1:5869734/150‑1 (MQ=255)

                                                                     gTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGgcg                                                                                 >  1:8580070/1‑150 (MQ=255)

                                                                     gTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGgcg                                                                                 >  2:1078169/1‑150 (MQ=255)

                                                                      ttGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGgcgc                                                                                <  2:1751956/150‑1 (MQ=255)

                                                                         gCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTg                                                                             <  1:3067856/150‑1 (MQ=255)

                                                                         gCAAGTTTGATGATCAGTTTGGACTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGAGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTg                                                                             >  2:5215757/1‑150 (MQ=255)

                                                                           aaGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTa                                                                           <  2:1262113/150‑1 (MQ=255)

                                                                           aaGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTa                                                                           <  1:8646211/150‑1 (MQ=255)

                                                                             gTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTAtt                                                                         >  2:3017310/1‑150 (MQ=255)

                                                                              tttGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTc                                                                        >  2:3771476/1‑150 (MQ=255)

                                                                              tttGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTc                                                                        >  1:2059836/1‑150 (MQ=255)

                                                                              tttGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTc                                                                        >  1:471077/1‑150 (MQ=255)

                                                                                tgatgaTCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGt                                                                      <  2:149771/150‑1 (MQ=255)

                                                                                tgatgaTCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGt                                                                      <  2:5889720/150‑1 (MQ=255)

                                                                                tgatgaTCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGt                                                                      <  1:8400313/150‑1 (MQ=255)

                                                                                tgatgaTCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGt                                                                      <  1:3462976/150‑1 (MQ=255)

                                                                                   tgatCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGaa                                                                   <  2:202922/150‑1 (MQ=255)

                                                                                    gatCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAAt                                                                  >  2:6088112/1‑150 (MQ=255)

                                                                                    gatCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAAt                                                                  >  2:2419403/1‑150 (MQ=255)

                                                                                      tCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATAc                                                                >  1:5630843/1‑150 (MQ=255)

                                                                                        aGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGt                                                              <  1:2154013/150‑1 (MQ=255)

                                                                                         gTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGtt                                                             >  1:632730/1‑150 (MQ=255)

                                                                                          tttGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGttt                                                            >  2:1662751/1‑150 (MQ=255)

                                                                                          tttGGCCTGATGGTAACTGGCAGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTAATCGTGAATACGttt                                                            >  2:3238083/1‑150 (MQ=255)

                                                                                               ccTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCt                                                       >  2:7306076/1‑150 (MQ=255)

                                                                                                  gATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGaa                                                    <  1:3801664/150‑1 (MQ=255)

                                                                                                  gATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGaa                                                    >  2:8272169/1‑150 (MQ=255)

                                                                                                   aTGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCTTGTATACGTTTTACCTGAAc                                                   >  2:5000615/1‑150 (MQ=255)

                                                                                                   aTGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAAc                                                   <  1:7429240/150‑1 (MQ=255)

                                                                                                    tGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACg                                                  >  2:4258885/1‑150 (MQ=255)

                                                                                                      gTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTc                                                <  1:8012452/150‑1 (MQ=255)

                                                                                                         aCTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGaa                                             <  1:3850841/150‑1 (MQ=255)

                                                                                                           tGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATa                                           >  1:7043557/1‑150 (MQ=255)

                                                                                                             gCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAgc                                         <  2:8860130/150‑1 (MQ=255)

                                                                                                              ccGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAgcg                                        >  1:5641846/1‑150 (MQ=255)

                                                                                                               cGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAgcgc                                       <  2:2650717/150‑1 (MQ=255)

                                                                                                               cGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAgcgc                                       >  1:3935738/1‑150 (MQ=255)

                                                                                                                     cTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCggg                                 <  1:676047/150‑1 (MQ=255)

                                                                                                                     cTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCggg                                 <  2:7714754/150‑1 (MQ=255)

                                                                                                                      ttGTCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGACTAGCGCCTCgggg                                >  1:8207627/1‑150 (MQ=255)

                                                                                                                      ttGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCgggg                                <  2:5497463/150‑1 (MQ=255)

                                                                                                                      ttGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGAGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCgggg                                <  1:1978467/150‑1 (MQ=255)

                                                                                                                        gCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGtt                              <  1:120134/150‑1 (MQ=255)

                                                                                                                        gCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGtt                              <  1:7527982/150‑1 (MQ=255)

                                                                                                                            gCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGAcc                          >  1:4183471/1‑150 (MQ=255)

                                                                                                                            gCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGAcc                          <  1:9121322/150‑1 (MQ=255)

                                                                                                                                aGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGtta                      <  1:7198126/150‑3 (MQ=255)

                                                                                                                                aGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGa                      <  2:2778937/150‑1 (MQ=255)

                                                                                                                                   tCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGc                   <  2:7141879/150‑1 (MQ=255)

                                                                                                                                     ttCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATa                                                                 >  2:4022777/1‑102 (MQ=255)

                                                                                                                                     ttCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCg                 >  2:4299190/1‑150 (MQ=255)

                                                                                                                                      tCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGa                <  1:5131459/150‑1 (MQ=255)

                                                                                                                                          ccGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCg            >  2:6352884/1‑150 (MQ=255)

                                                                                                                                          acctggCACCAACTGGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCg            >  1:2945507/5‑150 (MQ=255)

                                                                                                                                            ctggCACCAACTGGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGAt          >  1:1760557/3‑150 (MQ=255)

                                                                                                                                             tggCACCAACTGGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATc         >  1:3580966/2‑150 (MQ=255)

                                                                                                                                             tggCACCAACTGGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATc         >  2:76728/2‑150 (MQ=255)

                                                                                                                                             tggCACCAACTGGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATc         >  2:4741760/2‑150 (MQ=255)

                                                                                                                                               gCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATCCt       <  2:2536441/150‑1 (MQ=255)

                                                                                                                                                cACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATCCTg      >  1:2432316/1‑150 (MQ=255)

                                                                                                                                                cACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGACGATGCGATCCTg      <  1:6885649/150‑1 (MQ=255)

                                                                                                                                                 aCCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATCCTGa     >  2:853964/1‑150 (MQ=255)

                                                                                                                                                  cccAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATCCTGaa    <  1:9022518/150‑1 (MQ=255)

                                                                                                                                                   ccAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATCCTGAat   <  2:3212587/150‑1 (MQ=255)

                                                                                                                                                   ccAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATCCTGAat   >  1:3212587/1‑150 (MQ=255)

                                                                                                                                                   ccAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATCCTGAat   >  2:5689357/1‑150 (MQ=255)

GTTGGGCAGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATCCTGAATG  >  NC_002947/5745944‑5746241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`