Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,746,094 G→C 6.2% N583K (AAC→AAG) ‡ glgP ← glycogen phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,746,0940GC 6.2% 859.1 / 33.5 256N583K (AAC→AAG) ‡glgPglycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (121/119);  new base C (11/5);  total (132/124)
Fisher's exact test for biased strand distribution p-value = 1.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 259 total aligned reads displayed.

TTGGGCAGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATCCTGAATGAT  >  NC_002947/5745945‑5746243
                                                                                                                                                     |                                                                                                                                                     
ttGGGCAGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAg                                                                                                                                                       >  1:6131531/1‑150 (MQ=255)
 tGGGCAGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGt                                                                                                                                                      <  1:810268/150‑1 (MQ=255)
  gggCAGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGtt                                                                                                                                                     >  1:149771/1‑150 (MQ=255)
  gggCAGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGtt                                                                                                                                                     <  2:6769898/150‑1 (MQ=255)
    gCAGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTgg                                                                                                                                                   >  1:6809614/1‑150 (MQ=255)
     cAGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGt                                                                                                                                                  <  1:8977427/150‑1 (MQ=255)
      aGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTg                                                                                                                                                 >  1:7278742/1‑150 (MQ=255)
      aGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACAACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTg                                                                                                                                                 >  1:2643256/1‑150 (MQ=255)
         aaCACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCa                                                                                                                                              <  2:8334326/150‑1 (MQ=255)
            accaccTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGt                                                                                                                                           >  1:2536441/1‑150 (MQ=255)
             ccaccTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGtt                                                                                                                                          >  1:6558583/1‑150 (MQ=255)
                 cTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGtt                                                                                                                                      <  2:1846182/150‑1 (MQ=255)
                  ttCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTg                                                                                                                                     <  1:3806616/150‑1 (MQ=255)
                      gcagGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTa                                                                                                                                 <  2:1563650/150‑1 (MQ=255)
                        agGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATg                                                                                                                               >  2:6885649/1‑150 (MQ=255)
                             gcgcACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTg                                                                                                                          <  2:869234/150‑1 (MQ=255)
                             gcgcACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTg                                                                                                                          <  1:389262/150‑1 (MQ=255)
                               gcACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGt                                                                                                                        >  2:3420336/1‑150 (MQ=255)
                               gcACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGt                                                                                                                        <  1:8326156/150‑1 (MQ=255)
                               gcACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGt                                                                                                                        <  1:1597960/150‑1 (MQ=255)
                                   tGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAg                                                                                                                    >  1:1393919/1‑150 (MQ=255)
                                   tGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAg                                                                                                                    <  2:6456698/150‑1 (MQ=255)
                                       gggTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCa                                                                                                                <  1:6847973/150‑1 (MQ=255)
                                              tgttCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGgtgt                                                                                                         <  2:2168281/150‑1 (MQ=255)
                                              tgttCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGgtgt                                                                                                         <  1:8546782/150‑1 (MQ=255)
                                                ttCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGc                                                                                                       >  2:529297/1‑150 (MQ=255)
                                                  caccacCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCAt                                                                                                     >  1:2993531/1‑150 (MQ=255)
                                                   accaccCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATc                                                                                                    <  2:3036295/150‑1 (MQ=255)
                                                   accaccCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATc                                                                                                    >  1:3906176/1‑150 (MQ=255)
                                                   accaccCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATc                                                                                                    <  1:172105/150‑1 (MQ=255)
                                                        ccGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGtt                                                                                               >  1:4968243/1‑150 (MQ=255)
                                                         cGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTg                                                                                              >  1:7793151/1‑150 (MQ=255)
                                                          gggCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGa                                                                                             <  2:8620113/150‑1 (MQ=255)
                                                                  tCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTgg                                                                                     <  1:5869734/150‑1 (MQ=255)
                                                                  tCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTgg                                                                                     >  1:3158053/1‑150 (MQ=255)
                                                                   cGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGgc                                                                                    <  1:7195345/150‑1 (MQ=255)
                                                                     ttGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGgcgc                                                                                  <  2:1751956/150‑1 (MQ=255)
                                                                       ggCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCtt                                                                                <  2:3642994/150‑1 (MQ=255)
                                                                        gCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTg                                                                               <  2:7816191/150‑1 (MQ=255)
                                                                        gCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTg                                                                               <  1:3067856/150‑1 (MQ=255)
                                                                        gCAAGTTTGATGATCAGTTTGGACTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGAGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTg                                                                               >  2:5215757/1‑150 (MQ=255)
                                                                          aaGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTa                                                                             <  2:1262113/150‑1 (MQ=255)
                                                                          aaGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTa                                                                             <  1:8646211/150‑1 (MQ=255)
                                                                           aGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTAt                                                                            <  1:8375261/150‑1 (MQ=255)
                                                                             tttGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTc                                                                          <  2:2993531/150‑1 (MQ=255)
                                                                             tttGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTc                                                                          >  2:3771476/1‑150 (MQ=255)
                                                                             tttGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTc                                                                          >  1:471029/1‑150 (MQ=255)
                                                                             tttGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTc                                                                          <  2:6125374/150‑1 (MQ=255)
                                                                               tgatgaTCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGt                                                                        <  2:149771/150‑1 (MQ=255)
                                                                               tgatgaTCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGt                                                                        <  2:5889720/150‑1 (MQ=255)
                                                                                 atgatCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGa                                                                      <  1:3231414/150‑1 (MQ=255)
                                                                                  tgatCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGaa                                                                     <  1:3508017/150‑1 (MQ=255)
                                                                                  tgatCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGaa                                                                     <  2:202922/150‑1 (MQ=255)
                                                                                   gatCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAAt                                                                    >  2:2419403/1‑150 (MQ=255)
                                                                                    atCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATa                                                                   <  1:6494846/150‑1 (MQ=255)
                                                                                       aGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGt                                                                <  1:2154013/150‑1 (MQ=255)
                                                                                         tttGGCCTGATGGTAACTGGCAGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTAATCGTGAATACGttt                                                              >  2:3238083/1‑150 (MQ=255)
                                                                                              ccTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCt                                                         >  2:7306076/1‑150 (MQ=255)
                                                                                                 gATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGaa                                                      >  2:8272169/1‑150 (MQ=255)
                                                                                                  aTGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCTTGTATACGTTTTACCTGAAc                                                     >  2:5000615/1‑150 (MQ=255)
                                                                                                   tGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACg                                                    >  2:4258885/1‑150 (MQ=255)
                                                                                                     gTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTc                                                  <  1:8012452/150‑1 (MQ=255)
                                                                                                       aaCTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGa                                                >  1:4272639/1‑150 (MQ=255)
                                                                                                          tGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATa                                             <  1:6736453/150‑1 (MQ=255)
                                                                                                          tGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATa                                             >  1:7043557/1‑150 (MQ=255)
                                                                                                            gCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAgc                                           <  2:8860130/150‑1 (MQ=255)
                                                                                                            gCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAgc                                           <  1:3796618/150‑1 (MQ=255)
                                                                                                             ccGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAgcg                                          >  1:5641842/1‑150 (MQ=255)
                                                                                                              cGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAAGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAgcgc                                         >  1:577925/1‑150 (MQ=255)
                                                                                                                    cTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCggg                                   <  2:7714754/150‑1 (MQ=255)
                                                                                                                     ttGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCgggg                                  <  2:5497463/150‑1 (MQ=255)
                                                                                                                     ttGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGAGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCgggg                                  <  1:1978467/150‑1 (MQ=255)
                                                                                                                       gCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGtt                                <  1:120134/150‑1 (MQ=255)
                                                                                                                           gCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGAcc                            >  1:4183366/1‑150 (MQ=255)
                                                                                                                           gCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGAcc                            <  1:9121322/150‑1 (MQ=255)
                                                                                                                               aGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGa                        <  2:2778937/150‑1 (MQ=255)
                                                                                                                               aGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGa                        <  1:5111341/150‑1 (MQ=255)
                                                                                                                               aGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGa                        >  1:2086265/1‑150 (MQ=255)
                                                                                                                                gATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGAc                       <  1:5624207/150‑1 (MQ=255)
                                                                                                                                  tCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGc                     <  2:7141879/150‑1 (MQ=255)
                                                                                                                                  tCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGc                     >  1:7446011/1‑150 (MQ=255)
                                                                                                                                  tCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGc                     >  1:5274923/1‑150 (MQ=255)
                                                                                                                                    ttCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATa                                                                   >  2:4022777/1‑102 (MQ=255)
                                                                                                                                    ttCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATa                                                                   <  1:4022777/102‑1 (MQ=255)
                                                                                                                                    ttCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCg                   >  1:4568724/1‑150 (MQ=255)
                                                                                                                                    ttCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCg                   >  2:4299190/1‑150 (MQ=255)
                                                                                                                                    ttCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCg                   >  1:2911955/1‑150 (MQ=255)
                                                                                                                                         ccGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCg              >  2:6352884/1‑150 (MQ=255)
                                                                                                                                         acctggCACCAACTGGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCg              >  1:2945507/5‑150 (MQ=255)
                                                                                                                                           ctggCACCAACTGGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGAt            >  1:1760557/3‑150 (MQ=255)
                                                                                                                                            tggCACCAACTGGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATc           >  2:4741760/2‑150 (MQ=255)
                                                                                                                                            tggCACCAACTGGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATc           >  1:9158989/2‑150 (MQ=255)
                                                                                                                                            tggCACCAACTGGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATc           >  2:76728/2‑150 (MQ=255)
                                                                                                                                              gCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATCCt         <  2:2536441/150‑1 (MQ=255)
                                                                                                                                               cACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATCCTg        >  1:2432316/1‑150 (MQ=255)
                                                                                                                                                aCCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATCCTGa       >  2:853964/1‑150 (MQ=255)
                                                                                                                                                 cccAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATCCTGaa      <  2:2643256/150‑1 (MQ=255)
                                                                                                                                                  ccAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATCCTGAat     >  2:5689357/1‑150 (MQ=255)
                                                                                                                                                  ccAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATCCTGAat     <  2:3212587/150‑1 (MQ=255)
                                                                                                                                                     gttgttGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATCCTGAatgat  >  2:5837698/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTGGGCAGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATCCTGAATGAT  >  NC_002947/5745945‑5746243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑