Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,746,096 T→G 6.6% N583H (AAC→CAC) ‡ glgP ← glycogen phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,746,0960TG 6.6% 853.4 / 33.7 253N583H (AAC→CAC) ‡glgPglycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (120/116);  new base G (11/6);  total (131/122)
Fisher's exact test for biased strand distribution p-value = 3.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 257 total aligned reads displayed.

GGGCAGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATCCTGAATGAT  >  NC_002947/5745947‑5746243
                                                                                                                                                     |                                                                                                                                                   
gggCAGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGtt                                                                                                                                                     >  1:149771/1‑150 (MQ=255)
gggCAGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGtt                                                                                                                                                     <  2:6769898/150‑1 (MQ=255)
  gCAGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTgg                                                                                                                                                   >  1:6809614/1‑150 (MQ=255)
    aGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTg                                                                                                                                                 >  1:359066/1‑150 (MQ=255)
    aGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTg                                                                                                                                                 >  1:7278742/1‑150 (MQ=255)
    aGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACAACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTg                                                                                                                                                 >  1:2643256/1‑150 (MQ=255)
     ggAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGc                                                                                                                                                >  2:3658977/1‑150 (MQ=255)
      gAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGcc                                                                                                                                               >  1:6617950/1‑150 (MQ=255)
       aaCACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCa                                                                                                                                              <  2:8334326/150‑1 (MQ=255)
          accaccTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGt                                                                                                                                           >  1:2536441/1‑150 (MQ=255)
          accaccTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGt                                                                                                                                           >  1:2778937/1‑150 (MQ=255)
               cTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGtt                                                                                                                                      <  2:1846829/150‑1 (MQ=255)
                ttCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTg                                                                                                                                     >  1:915003/1‑150 (MQ=255)
                ttCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTg                                                                                                                                     <  1:3806616/150‑1 (MQ=255)
                 tCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGc                                                                                                                                    <  2:1779798/150‑1 (MQ=255)
                    gcagGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTa                                                                                                                                 >  2:8001118/1‑150 (MQ=255)
                      agGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATg                                                                                                                               <  2:6948057/150‑1 (MQ=255)
                           gcgcACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTg                                                                                                                          <  2:869234/150‑1 (MQ=255)
                             gcACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGt                                                                                                                        >  2:3420336/1‑150 (MQ=255)
                             gcACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGt                                                                                                                        <  1:1597960/150‑1 (MQ=255)
                                cTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACa                                                                                                                     <  1:5859241/150‑1 (MQ=255)
                                 tGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAg                                                                                                                    >  1:1393919/1‑150 (MQ=255)
                                     gggTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCa                                                                                                                <  1:6847973/150‑1 (MQ=255)
                                              ttCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGc                                                                                                       >  2:5387172/1‑150 (MQ=255)
                                                 accaccCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATc                                                                                                    >  1:3906176/1‑150 (MQ=255)
                                                 accaccCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATc                                                                                                    <  1:172105/150‑1 (MQ=255)
                                                   caccCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAg                                                                                                  >  2:2072243/1‑150 (MQ=255)
                                                   caccCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAg                                                                                                  >  2:6714422/1‑150 (MQ=255)
                                                      ccGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGtt                                                                                               <  2:4815601/150‑1 (MQ=255)
                                                       cGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTg                                                                                              >  1:7793151/1‑150 (MQ=255)
                                                        gggCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGa                                                                                             <  1:5070960/150‑1 (MQ=255)
                                                        gggCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGa                                                                                             <  1:2981211/150‑1 (MQ=255)
                                                        gggCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGa                                                                                             <  2:8620113/150‑1 (MQ=255)
                                                                tCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCTGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTgg                                                                                     >  2:22861/1‑150 (MQ=255)
                                                                tCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTgg                                                                                     >  1:3158053/1‑150 (MQ=255)
                                                                 cGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGgc                                                                                    <  1:7195345/150‑1 (MQ=255)
                                                                  gTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGgcg                                                                                   >  2:3071405/1‑150 (MQ=255)
                                                                  gTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGgcg                                                                                   >  1:8580070/1‑150 (MQ=255)
                                                                  gTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGgcg                                                                                   >  2:1078169/1‑150 (MQ=255)
                                                                   ttGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGgcgc                                                                                  <  1:4937772/150‑1 (MQ=255)
                                                                   ttGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGgcgc                                                                                  >  2:1330565/1‑150 (MQ=255)
                                                                      gCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTg                                                                               >  2:5729178/1‑150 (MQ=255)
                                                                      gCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTg                                                                               <  1:3067856/150‑1 (MQ=255)
                                                                      gCAAGTTTGATGATCAGTTTGGACTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGAGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTg                                                                               >  2:5215757/1‑150 (MQ=255)
                                                                          gTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTAtt                                                                           >  2:3017310/1‑150 (MQ=255)
                                                                           tttGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTc                                                                          <  2:6125374/150‑1 (MQ=255)
                                                                           tttGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTc                                                                          >  1:2059836/1‑150 (MQ=255)
                                                                           tttGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTc                                                                          >  1:471029/1‑150 (MQ=255)
                                                                             tgatgaTCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGt                                                                        <  1:8400313/150‑1 (MQ=255)
                                                                             tgatgaTCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGt                                                                        <  2:149771/150‑1 (MQ=255)
                                                                                tgatCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGaa                                                                     <  1:3508017/150‑1 (MQ=255)
                                                                                 gatCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAAt                                                                    >  2:2419453/1‑150 (MQ=255)
                                                                                  atCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATa                                                                   <  1:6494846/150‑1 (MQ=255)
                                                                                     aGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGt                                                                <  1:2154013/150‑1 (MQ=255)
                                                                                       tttGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGttt                                                              >  2:1662751/1‑150 (MQ=255)
                                                                                          ggCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAAc                                                           <  1:4115518/150‑1 (MQ=255)
                                                                                           gCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAAcc                                                          >  1:4511216/1‑150 (MQ=255)
                                                                                            ccTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCt                                                         >  2:7306076/1‑150 (MQ=255)
                                                                                             cTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTg                                                        >  1:8327815/1‑150 (MQ=255)
                                                                                               gATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGaa                                                      >  2:8272169/1‑150 (MQ=255)
                                                                                                aTGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCTTGTATACGTTTTACCTGAAc                                                     >  2:5000615/1‑150 (MQ=255)
                                                                                                   gTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTc                                                  <  1:8012452/150‑1 (MQ=255)
                                                                                                     aaCTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGa                                                >  1:4272639/1‑150 (MQ=255)
                                                                                                        tGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATa                                             >  1:7043557/1‑150 (MQ=255)
                                                                                                        tGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATa                                             <  1:6736453/150‑1 (MQ=255)
                                                                                                          gCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAgc                                           <  2:8860130/150‑1 (MQ=255)
                                                                                                           ccGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAgcg                                          >  1:5641842/1‑150 (MQ=255)
                                                                                                            cGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAgcgc                                         <  2:2650717/150‑1 (MQ=255)
                                                                                                                gCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCg                                     <  2:3251998/150‑1 (MQ=255)
                                                                                                                 ccTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCgg                                    >  1:3857337/1‑150 (MQ=255)
                                                                                                                  cTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCggg                                   <  2:5974294/150‑1 (MQ=255)
                                                                                                                  cTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCggg                                   <  2:7714754/150‑1 (MQ=255)
                                                                                                                  cTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCggg                                   >  1:8707247/1‑150 (MQ=255)
                                                                                                                   ttGTCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGACTAGCGCCTCgggg                                  >  1:8207627/1‑150 (MQ=255)
                                                                                                                   ttGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCgggg                                  <  2:5497463/150‑1 (MQ=255)
                                                                                                                   ttGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGAGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCgggg                                  <  1:1978467/150‑1 (MQ=255)
                                                                                                                     gCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGtt                                <  1:7527982/150‑1 (MQ=255)
                                                                                                                     gCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGtt                                <  1:120134/150‑1 (MQ=255)
                                                                                                                           gAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGt                          <  1:6080424/150‑1 (MQ=255)
                                                                                                                            aaGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTg                         >  2:4276264/1‑150 (MQ=255)
                                                                                                                               aTCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACg                      >  2:3835884/1‑150 (MQ=255)
                                                                                                                                tCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGc                     <  2:7141879/150‑1 (MQ=255)
                                                                                                                                tCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGc                     >  1:5274923/1‑150 (MQ=255)
                                                                                                                                  ttCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATa                                                                   >  2:4022777/1‑102 (MQ=255)
                                                                                                                                  ttCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATa                                                                   <  1:4022777/102‑1 (MQ=255)
                                                                                                                                  ttCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCg                   >  2:4299190/1‑150 (MQ=255)
                                                                                                                                  ttCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCg                   >  1:5770554/1‑150 (MQ=255)
                                                                                                                                  ttCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCg                   >  1:551956/1‑150 (MQ=255)
                                                                                                                                       ccGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCg              >  2:6352884/1‑150 (MQ=255)
                                                                                                                                       acctggCACCAACTGGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCg              >  1:2945507/5‑150 (MQ=255)
                                                                                                                                         ctggCACCAACTGGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGAt            >  1:1760557/3‑150 (MQ=255)
                                                                                                                                          tggCACCAACTGGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATc           >  2:76728/2‑150 (MQ=255)
                                                                                                                                          cgGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATc           <  2:283371/150‑1 (MQ=255)
                                                                                                                                            gCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATCCt         <  2:2536441/150‑1 (MQ=255)
                                                                                                                                             cACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATCCTg        >  1:2432316/1‑150 (MQ=255)
                                                                                                                                              aCCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATCCTGa       >  2:853964/1‑150 (MQ=255)
                                                                                                                                               cccAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATCCTGaa      <  1:9022518/150‑1 (MQ=255)
                                                                                                                                                ccAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATCCTGAat     <  2:3212587/150‑1 (MQ=255)
                                                                                                                                                ccAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATCCTGAat     >  1:3212587/1‑150 (MQ=255)
                                                                                                                                                   gttgttGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATCCTGAatgat  >  2:5837698/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GGGCAGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGGCGATGTCGTTGGCAAGTTTGATGATCAGTTTGGCCTGATGGTAACTGGCCGCAGCCTTGCCAGCGAAGATCTTCACCCGCGGCACCCAGTTGGTGCCAGGTTCGTTGCGTATGGCCTGGTACAGTGCAACGGTGTGCATCAGGTTGAGCAACTGGCGCTTGTATTCGTGAATACGTTTAACCTGAACGTCGAATAGCGCCTCGGGGTTGACCGTGACGCCGATGCGATCCTGAATGAT  >  NC_002947/5745947‑5746243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑