BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,754,859	G→T	5.6%	S283S (TCG→TCT) ‡	glnA →	glutamine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,754,859	0	G	T	5.6%	823.8 / 24.5	244	S283S (TCG→TCT) ‡	glnA	glutamine synthetase
Reads supporting (aligned to +/- strand): ref base G (102/128); new base T (8/6); total (110/134)
Fisher's exact test for biased strand distribution p-value = 4.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 253 total aligned reads displayed.

GAAGTACAGGCGCTGAAATACGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTG  >  NC_002947/5754710‑5755006

gAAGTACAGGCGCTGAAATACGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCg                                                                                                                                                     <  2:1226561/150‑1 (MQ=255)

 aaGTACAGGCGCTGAAATACGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCgg                                                                                                                                                    <  1:1255436/150‑1 (MQ=255)

 aaGTACAGGCGCTGAAATACGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCgg                                                                                                                                                    >  1:2658964/1‑150 (MQ=255)

 aaGTACAGGCGCTGAAATACGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCgg                                                                                                                                                    <  2:7870694/150‑1 (MQ=255)

      cAGGCGCTGAAATACGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGaa                                                                                                                                               <  2:2588492/150‑1 (MQ=255)

      cAGGCGCTGAAATACGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGaa                                                                                                                                               <  2:6206562/150‑1 (MQ=255)

      cAGGCGCTGAAATACGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGaa                                                                                                                                               <  1:2972120/150‑1 (MQ=255)

      cAGGCGCTGAAATACGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGaa                                                                                                                                               >  1:3866797/1‑150 (MQ=255)

       aGGCGCTGAAATACGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAg                                                                                                                                              <  1:7348374/150‑1 (MQ=255)

             tGAAATACGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATTTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATg                                                                                                                                        >  1:8027932/1‑150 (MQ=255)

             tGAAATACGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAg                                                                                                                                              <  2:5620340/144‑1 (MQ=255)

               aaaTACGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGcc                                                                                                                                      >  1:8486447/1‑150 (MQ=255)

                aaTACGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGTAAAGACGTCAAGAACACCTTCTCGGGTGAAGGCTATGCCg                                                                                                                                     <  1:6313560/150‑1 (MQ=255)

                aaTACGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCg                                                                                                                                     <  2:1992771/150‑1 (MQ=255)

                 aTACGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCgg                                                                                                                                    <  1:5891930/150‑1 (MQ=255)

                   aCGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGcc                                                                                                                                  <  2:2658964/150‑1 (MQ=255)

                    cgtcgtGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCt                                                                                                                                 <  1:1086412/150‑1 (MQ=255)

                        gtGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTcc                                                                                                                             <  1:6667327/150‑1 (MQ=255)

                        gtGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTcc                                                                                                                             <  1:8976798/150‑1 (MQ=255)

                              aaCGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACAcc                                                                                                                       >  2:8145657/1‑150 (MQ=255)

                                 gTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGcc                                                                                                                    <  2:3929337/150‑1 (MQ=255)

                                  ttGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGccc                                                                                                                   >  1:6721017/1‑150 (MQ=255)

                                   tGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCt                                                                                                                  <  2:8207076/150‑1 (MQ=255)

                                     ccGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCGTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGt                                                                                                                <  2:8070140/150‑1 (MQ=255)

                                       gACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTAc                                                                                                              >  1:2430750/1‑150 (MQ=255)

                                       gACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTAc                                                                                                              >  2:5105179/1‑150 (MQ=255)

                                       gACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTAc                                                                                                              <  2:8885988/150‑1 (MQ=255)

                                        aCGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACt                                                                                                             <  1:3031655/150‑1 (MQ=255)

                                          gCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTc                                                                                                           >  2:6771977/1‑150 (MQ=255)

                                           cTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCa                                                                                                          <  2:4477915/150‑1 (MQ=255)

                                            ttACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCAt                                                                                                         >  2:3189802/1‑150 (MQ=255)

                                            ttACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCAt                                                                                                         >  2:8292336/1‑150 (MQ=255)

                                              aCGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATcg                                                                                                       >  1:4113351/1‑150 (MQ=255)

                                                 gCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATcggcg                                                                                                    <  2:1964788/150‑1 (MQ=255)

                                                 gCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATcggcg                                                                                                    <  2:9028863/150‑1 (MQ=255)

                                                      aCCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACCTGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTatc                                                                                               <  2:7381115/150‑1 (MQ=255)

                                                      aCCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTatc                                                                                               <  2:8095114/150‑1 (MQ=255)

                                                          ccACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTAtcatca                                                                                           <  1:8794592/150‑1 (MQ=255)

                                                           cACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCaa                                                                                          <  2:5436923/150‑1 (MQ=255)

                                                              cTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCa                                                                                       >  2:3699954/1‑150 (MQ=255)

                                                               ttCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCAc                                                                                      <  1:6918765/150‑1 (MQ=255)

                                                                tCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACg                                                                                     <  1:2068900/150‑1 (MQ=255)

                                                                  aTGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGt                                                                                   <  1:4246701/150‑1 (MQ=255)

                                                                     ccGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAg                                                                                >  2:2490324/1‑150 (MQ=255)

                                                                     ccGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAg                                                                                >  1:4501944/1‑150 (MQ=255)

                                                                       gAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGc                                                                              <  1:4725876/150‑1 (MQ=255)

                                                                            cACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGTAAGACGGCAAGAACACATTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGa                                                                         >  1:559471/1‑150 (MQ=255)

                                                                            cACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGa                                                                         >  1:255166/1‑150 (MQ=255)

                                                                            cACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGa                                                                         >  2:15028/1‑150 (MQ=255)

                                                                             aCTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGaa                                                                        <  2:5046602/150‑1 (MQ=255)

                                                                              cTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAAc                                                                       <  1:5890013/150‑1 (MQ=255)

                                                                                  aCGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCt                                                                   >  1:9141147/1‑150 (MQ=255)

                                                                                    ggCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTc                                                                 >  1:9038993/1‑150 (MQ=255)

                                                                                    ggCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTc                                                                 >  1:4992155/1‑150 (MQ=255)

                                                                                     gCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCa                                                                >  2:1754754/1‑150 (MQ=255)

                                                                                     gCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCa                                                                <  2:3412995/150‑1 (MQ=255)

                                                                                      cGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCAc                                                               >  1:2773890/1‑150 (MQ=255)

                                                                                         cAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCaa                                                            <  1:4053472/150‑1 (MQ=255)

                                                                                          aaCGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAAc                                                           <  1:592552/150‑1 (MQ=255)

                                                                                            cGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTGCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCagggt                                                         <  2:7888890/150‑5 (MQ=255)

                                                                                               cTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTc                                                      >  1:3699503/1‑150 (MQ=255)

                                                                                                  gggCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGAc                                                   >  2:7067801/1‑150 (MQ=255)

                                                                                                    gCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCa                                                 >  2:2700674/1‑150 (MQ=255)

                                                                                                    gCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCa                                                 >  1:3344512/1‑150 (MQ=255)

                                                                                                        gCACGTTCACTTCTCGATCTGGAAAGAAGGCAAGCCAACGTTTGCTGGTAACGAATATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTc                                             <  1:7802975/150‑1 (MQ=255)

                                                                                                        gCACGTGCACATGTCGATCTGGCAAGACGGCAAGAACACCGTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCGGATCGGCTTCACCAACCCGTCGACCAACTc                                             <  2:3502006/150‑1 (MQ=255)

                                                                                                        gCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTc                                             <  2:3584255/150‑1 (MQ=255)

                                                                                                        gCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTc                                             >  2:6135711/1‑150 (MQ=255)

                                                                                                        gCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTc                                             >  1:8007672/1‑150 (MQ=255)

                                                                                                        gCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTc                                             >  2:4535997/1‑150 (MQ=255)

                                                                                                          aCGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGt                                           >  2:485209/1‑150 (MQ=255)

                                                                                                            gTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTAc                                         <  2:6570508/150‑1 (MQ=255)

                                                                                                              gCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTCTCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACac                                       >  2:2221201/1‑149 (MQ=255)

                                                                                                                atttctCGATCTGGAAAGAAGGCAAGCCAACCTTCGCTGGTAACGAATATGCCGGCCTGTCGGAAAACTGCCTTTACTTCATCGGTGGCGTCATCAAGCACGCCAAGGCCGTGAATGCCTTCACCAACCCGTCGACCAACTCTTACAAGc                                     >  1:4428095/6‑150 (MQ=255)

                                                                                                                acaTGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGc                                     <  2:7383579/150‑1 (MQ=255)

                                                                                                                  ttctCGATCTGGAAAGAAGGCAAGCCAACGTTTGCTGGTAACGAATATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGt                                   >  2:4643576/4‑150 (MQ=255)

                                                                                                                  ttctCGATCTGGAAAGAAGGCAAGCCAACGTTTGCTGGTAACGAATATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGt                                   >  1:5345173/4‑150 (MQ=255)

                                                                                                                  ttctCGATCTGGAAAGAAGGCAAGCCAACGTTTGCTGGTAACGAATATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGt                                   >  1:6062385/4‑150 (MQ=255)

                                                                                                                  aTGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGt                                   >  1:639899/1‑150 (MQ=255)

                                                                                                                   tctCGATCTGGAAAGAAGGCAAGCCAACGTTTGCTGGTAACGAATATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTc                                  <  2:7767449/148‑1 (MQ=255)

                                                                                                                   tGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTc                                  <  2:7966092/150‑1 (MQ=255)

                                                                                                                    gTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCt                                 <  2:4913712/150‑1 (MQ=255)

                                                                                                                     tCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATcggcg                                                                                                    >  2:7114732/1‑82 (MQ=255)

                                                                                                                     tCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATcggcg                                                                                                    <  1:7114732/82‑1 (MQ=255)

                                                                                                                      cGATCTGGAAAGAAGGCAAGCCAACGTTTGCTGGTAACGAATATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTCTgg                               >  1:4355239/1‑150 (MQ=255)

                                                                                                                           tGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCa                          >  2:3211661/1‑150 (MQ=255)

                                                                                                                           tGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCa                          <  2:226433/150‑1 (MQ=255)

                                                                                                                            ggAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAg                         <  2:5812294/150‑1 (MQ=255)

                                                                                                                             gAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAgg                        >  2:179831/1‑150 (MQ=255)

                                                                                                                               aaGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGAcc                                                  <  2:4444198/122‑1 (MQ=255)

                                                                                                                               aaGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCt                      >  2:5356468/1‑150 (MQ=255)

                                                                                                                                    ggCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGaa                 >  1:5627071/1‑150 (MQ=255)

                                                                                                                                    ggCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGaa                 >  1:8806052/1‑150 (MQ=255)

                                                                                                                                    ggCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGaa                 <  1:8202996/150‑1 (MQ=255)

                                                                                                                                    ggCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGaa                 >  1:1812360/1‑150 (MQ=255)

                                                                                                                                       aaGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGcc              <  2:6419893/150‑1 (MQ=255)

                                                                                                                                            cacCTTCTCGGGTGAAGGCTATTCCGGCCTGTCCGACACCGCCCTGTACTTCATcggc                                                                                                     <  1:5171900/58‑1 (MQ=255)

                                                                                                                                            cacCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGt         <  1:7519632/150‑1 (MQ=255)

                                                                                                                                             acCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTa        >  1:3167061/1‑150 (MQ=255)

                                                                                                                                               cTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAAt      <  1:5105179/150‑1 (MQ=255)

GAAGTACAGGCGCTGAAATACGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTG  >  NC_002947/5754710‑5755006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`