BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,754,888	C→A	9.4%	T293N (ACC→AAC)	glnA →	glutamine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,754,888	0	C	A	9.4%	752.9 / 51.6	233	T293N (ACC→AAC)	glnA	glutamine synthetase
Reads supporting (aligned to +/- strand): ref base C (106/105); new base A (15/7); total (121/112)
Fisher's exact test for biased strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 246 total aligned reads displayed.

AACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGA  >  NC_002947/5754740‑5755037

aaCGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACAcc                                                                                                                                                      >  2:8145657/1‑150 (MQ=255)

  cGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGc                                                                                                                                                    >  2:7796225/1‑150 (MQ=255)

   gTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGcc                                                                                                                                                   <  2:3929337/150‑1 (MQ=255)

     tGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCt                                                                                                                                                 <  2:8207076/150‑1 (MQ=255)

       ccGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCGTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGt                                                                                                                                               <  2:8070140/150‑1 (MQ=255)

         gACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTAc                                                                                                                                             <  2:3828618/150‑1 (MQ=255)

         gACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTAc                                                                                                                                             >  1:6673800/1‑150 (MQ=255)

            gCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTc                                                                                                                                          >  2:6771977/1‑150 (MQ=255)

              ttACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCAt                                                                                                                                        >  2:8292336/1‑150 (MQ=255)

              ttACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCAt                                                                                                                                        >  2:3189802/1‑150 (MQ=255)

                aCGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATcg                                                                                                                                      >  1:4113351/1‑150 (MQ=255)

                  ggCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACGCCGCCCTGTACTTCATcggc                                                                                                                                    >  1:7489135/1‑150 (MQ=255)

                   gCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATcggcg                                                                                                                                   >  1:4400664/1‑150 (MQ=255)

                   gCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATcggcg                                                                                                                                   <  2:1964788/150‑1 (MQ=255)

                   gCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATcggcg                                                                                                                                   <  2:9028863/150‑1 (MQ=255)

                        aCCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTatc                                                                                                                              <  2:8095114/150‑1 (MQ=255)

                        aCCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTatc                                                                                                                              <  2:1452978/150‑1 (MQ=255)

                           gCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTatcatc                                                                                                                           <  1:6784129/150‑1 (MQ=255)

                            ccACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTAtcatca                                                                                                                          <  1:8794592/150‑1 (MQ=255)

                             cACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCaa                                                                                                                         <  2:5436923/150‑1 (MQ=255)

                              aCCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAg                                                                                                                        >  1:5530853/1‑150 (MQ=255)

                                cTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCa                                                                                                                      >  2:3699954/1‑150 (MQ=255)

                                 ttCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCAc                                                                                                                     >  2:5752857/1‑150 (MQ=255)

                                  tCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACg                                                                                                                    <  2:1544040/150‑1 (MQ=255)

                                    aTGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGt                                                                                                                  <  1:4246701/150‑1 (MQ=255)

                                    aTGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGt                                                                                                                  <  2:4749754/150‑1 (MQ=255)

                                     tGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCAt                                                                                                                                        >  1:1814116/1‑127 (MQ=255)

                                      gCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTaa                                                                                                                <  1:3051303/150‑1 (MQ=255)

                                       ccGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAg                                                                                                               >  2:2490324/1‑150 (MQ=255)

                                              cACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGa                                                                                                        >  1:255166/1‑150 (MQ=255)

                                                   tACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGc                                                                                                   <  1:3959276/150‑1 (MQ=255)

                                                     cGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCtt                                                                                                 <  2:2558774/150‑1 (MQ=255)

                                                      ggCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTCTCATCAAGCACGGTACGGCCCTGAACGGCTTc                                                                                                >  2:4923185/1‑150 (MQ=255)

                                                      ggCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTc                                                                                                >  1:9038993/1‑150 (MQ=255)

                                                       gCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCa                                                                                               <  2:3413046/150‑1 (MQ=255)

                                                        cGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCAc                                                                                              <  2:8883328/150‑1 (MQ=255)

                                                          aCAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCa                                                                                            >  1:2876109/1‑150 (MQ=255)

                                                            aaCGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAAc                                                                                          <  1:592552/150‑1 (MQ=255)

                                                            aaCGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAAc                                                                                          <  1:1203923/150‑1 (MQ=255)

                                                                    gggCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGAc                                                                                  >  2:7067801/1‑150 (MQ=255)

                                                                    gggCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGAc                                                                                  >  2:2056690/1‑150 (MQ=255)

                                                                    gggCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGAc                                                                                  <  1:5918865/150‑1 (MQ=255)

                                                                      gCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCa                                                                                >  1:3344512/1‑150 (MQ=255)

                                                                        aTGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAAc                                                                              <  1:4628033/150‑1 (MQ=255)

                                                                          gCACGTTCACTTCTCGATCTGGAAAGAAGGCAAGCCAACGTTTGCTGGTAACGAATATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTc                                                                            <  1:7802975/150‑1 (MQ=255)

                                                                          gCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTc                                                                            <  1:1297399/150‑1 (MQ=255)

                                                                          gCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTc                                                                            >  2:4535997/1‑150 (MQ=255)

                                                                          gCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTc                                                                            >  2:6135711/1‑150 (MQ=255)

                                                                          gCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTc                                                                            <  2:3502753/150‑1 (MQ=255)

                                                                           cACGTTCACTTCTCGATCTGGAAAGAAGGCAAGCCAACGTTTGCTGGTAACGAATATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTcc                                                                           >  1:2240410/1‑149 (MQ=255)

                                                                            aCGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGt                                                                          <  1:9117315/150‑1 (MQ=255)

                                                                                gCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTCTCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACac                                                                      >  2:2221201/1‑149 (MQ=255)

                                                                                  acaTGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGc                                                                    <  2:7383579/150‑1 (MQ=255)

                                                                                  acaTGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGc                                                                    >  2:2676344/1‑150 (MQ=255)

                                                                                    ttctCGATCTGGAAAGAAGGCAAGCCAACGTTTGCTGGTAACGAATATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGt                                                                  >  2:4643576/4‑150 (MQ=255)

                                                                                    aTGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGt                                                                  >  1:639899/1‑150 (MQ=255)

                                                                                    aTGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGt                                                                  <  1:3767038/150‑1 (MQ=255)

                                                                                    aTGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGt                                                                  <  2:6491857/150‑1 (MQ=255)

                                                                                     tctCGATCTGGAAAGAAGGCAAGCCAACGTTTGCTGGTAACGAATATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTc                                                                 <  2:8009576/148‑1 (MQ=255)

                                                                                     tGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTc                                                                 <  1:3358148/150‑1 (MQ=255)

                                                                                     tGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTc                                                                 <  1:5128402/150‑1 (MQ=255)

                                                                                      gTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCt                                                                <  2:4913690/150‑1 (MQ=255)

                                                                                       tCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATcggcg                                                                                                                                   >  2:7114732/1‑82 (MQ=255)

                                                                                       tCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTg                                                               <  1:8024644/150‑1 (MQ=255)

                                                                                             tGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCa                                                         >  2:3211661/1‑150 (MQ=255)

                                                                                              ggAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAg                                                        <  1:5068510/150‑1 (MQ=255)

                                                                                              ggAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAg                                                        <  2:9014209/150‑1 (MQ=255)

                                                                                               gAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAgg                                                       >  2:179831/1‑150 (MQ=255)

                                                                                               gAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAgg                                                       >  2:7942569/1‑150 (MQ=255)

                                                                                                 aaGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGAcc                                                                                 <  2:4444198/122‑1 (MQ=255)

                                                                                                 aaGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGAcc                                                                                 >  1:4444198/1‑122 (MQ=255)

                                                                                                 aaGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCt                                                     >  2:5356468/1‑150 (MQ=255)

                                                                                                   gACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTc                                                   >  2:5954219/1‑150 (MQ=255)

                                                                                                      ggCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGaa                                                <  1:8202996/150‑1 (MQ=255)

                                                                                                      ggCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGaa                                                >  1:5627071/1‑150 (MQ=255)

                                                                                                      ggCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGaa                                                >  1:8579316/1‑150 (MQ=255)

                                                                                                      ggCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGaa                                                >  1:8806052/1‑150 (MQ=255)

                                                                                                           gAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGcccc                                           >  1:7947263/1‑150 (MQ=255)

                                                                                                              cacCTTCTCGGGTGAAGGCTATTCCGGCCTGTCCGACACCGCCCTGTACTTCATcggc                                                                                                                                    <  1:5171900/58‑1 (MQ=255)

                                                                                                              cacCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATcggc                                                                                                                                    >  2:5171900/1‑58 (MQ=255)

                                                                                                               acCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTa                                       >  1:3167061/1‑150 (MQ=255)

                                                                                                               acCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTa                                       >  1:7015415/1‑150 (MQ=255)

                                                                                                               acCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTa                                       >  2:7568892/1‑150 (MQ=255)

                                                                                                                 cTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAAt                                     >  1:2826393/1‑150 (MQ=255)

                                                                                                                    ctcGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCt                                  <  1:4953638/150‑1 (MQ=255)

                                                                                                                     tcGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTg                                 <  1:6226326/150‑1 (MQ=255)

                                                                                                                          taacgaatATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTCTGGTCCCAGGTTATGAAGCTCCGGTTCTGCTGGCCTa                            >  2:5069032/8‑150 (MQ=255)

                                                                                                                           gAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTAc                           >  1:19972/1‑150 (MQ=255)

                                                                                                                              gaatATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTCTGGTCCCAGGTTATGAAGCTCCGGTTCTGCTGGCCTATTCg                        >  2:2745971/4‑150 (MQ=255)

                                                                                                                              gaatATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTCTGGTCCCAGGTTATGAAGCTCCGGTTCTGCTGGCCTATTCg                        >  2:6274571/4‑150 (MQ=255)

                                                                                                                              gaatATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTCTGGTCCCAGGTTATGAAGCTCCGGTTCTGCTGGCCTATTCg                        >  2:423779/4‑150 (MQ=255)

                                                                                                                               gCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCgg                       >  2:3654790/1‑150 (MQ=255)

                                                                                                                                 tATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCt                     >  1:524015/1‑150 (MQ=255)

                                                                                                                                  aTGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTc                    <  1:4287097/150‑1 (MQ=255)

                                                                                                                                        gCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTCTGGTCCCAGGTTATGAAGCTCCGGTTCTGCTGGCCTATTCGGCTCGTAAcc              >  2:6381485/1‑150 (MQ=255)

                                                                                                                                                gACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGcc      >  2:2336533/1‑150 (MQ=255)

                                                                                                                                                  cacCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTc    >  1:3672968/1‑150 (MQ=255)

                                                                                                                                                  cacCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTc    <  2:6673684/150‑1 (MQ=255)

                                                                                                                                                  cacCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTc    <  1:1754754/150‑1 (MQ=255)

                                                                                                                                                  cacCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTc    <  2:4501944/150‑1 (MQ=255)

AACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGA  >  NC_002947/5754740‑5755037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`