BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,754,897	A→T	8.5%	Y296F (TAC→TTC)	glnA →	glutamine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,754,897	0	A	T	8.5%	771.1 / 45.2	246	Y296F (TAC→TTC)	glnA	glutamine synthetase
Reads supporting (aligned to +/- strand): ref base A (113/112); new base T (13/8); total (126/120)
Fisher's exact test for biased strand distribution p-value = 3.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 250 total aligned reads displayed.

CGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTC  >  NC_002947/5754748‑5755046

cGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTa                                                                                                                                                       >  1:2214270/1‑150 (MQ=255)

 gACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTAc                                                                                                                                                      >  2:5105179/1‑150 (MQ=255)

 gACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTAc                                                                                                                                                      >  1:821043/1‑150 (MQ=255)

 gACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTAc                                                                                                                                                      >  1:6673684/1‑150 (MQ=255)

 gACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTAc                                                                                                                                                      >  1:2430750/1‑150 (MQ=255)

      ttACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCAt                                                                                                                                                 >  2:3189802/1‑150 (MQ=255)

        aCGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATcg                                                                                                                                               >  1:262615/1‑150 (MQ=255)

           gCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATcggcg                                                                                                                                            <  2:1964788/150‑1 (MQ=255)

           gCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATcggcg                                                                                                                                            <  2:9028863/150‑1 (MQ=255)

           gCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATcggcg                                                                                                                                            <  1:3087989/150‑1 (MQ=255)

                aCCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACCTGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTatc                                                                                                                                       <  2:7381115/150‑1 (MQ=255)

                aCCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTatc                                                                                                                                       <  2:1452978/150‑1 (MQ=255)

                aCCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTatc                                                                                                                                       <  2:6056481/150‑1 (MQ=255)

                    ccACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTAtcatca                                                                                                                                   <  1:2980471/150‑1 (MQ=255)

                    ccACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTAtcatca                                                                                                                                   <  1:8794592/150‑1 (MQ=255)

                     cACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCaa                                                                                                                                  <  2:3552229/150‑1 (MQ=255)

                     cACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCaa                                                                                                                                  <  2:5436923/150‑1 (MQ=255)

                      aCCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAg                                                                                                                                 >  1:5530853/1‑150 (MQ=255)

                         ttCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCAc                                                                                                                              <  1:6918765/150‑1 (MQ=255)

                          tCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACg                                                                                                                             <  2:1544040/150‑1 (MQ=255)

                               ccGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAg                                                                                                                        >  2:2490324/1‑150 (MQ=255)

                               ccGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAg                                                                                                                        >  1:4501944/1‑150 (MQ=255)

                                   aGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGccc                                                                                                                    <  2:6633019/150‑1 (MQ=255)

                                       aCTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGaa                                                                                                                >  1:4166543/1‑150 (MQ=255)

                                           tACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGc                                                                                                            <  1:3959276/150‑1 (MQ=255)

                                              ggCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTc                                                                                                         >  2:4922933/1‑150 (MQ=255)

                                              ggCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTc                                                                                                         >  1:9038993/1‑150 (MQ=255)

                                               gCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCa                                                                                                        <  2:3413046/150‑1 (MQ=255)

                                                cGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCAc                                                                                                       <  2:7090937/150‑1 (MQ=255)

                                                cGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCAc                                                                                                       <  2:8883328/150‑1 (MQ=255)

                                                   cAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCaa                                                                                                    <  1:4053472/150‑1 (MQ=255)

                                                    aaCGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAAc                                                                                                   <  1:1203923/150‑1 (MQ=255)

                                                      cGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAAccc                                                                                                 >  2:4515886/1‑150 (MQ=255)

                                                      cGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTGCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCagggt                                                                                                 <  2:7888890/150‑5 (MQ=255)

                                                         cTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTc                                                                                              >  1:3699503/1‑150 (MQ=255)

                                                          tCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCg                                                                                             >  2:8121225/1‑150 (MQ=255)

                                                            gggCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGAc                                                                                           >  2:2056690/1‑150 (MQ=255)

                                                            gggCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGAc                                                                                           <  1:5918865/150‑1 (MQ=255)

                                                              gCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCa                                                                                         >  1:3344512/1‑150 (MQ=255)

                                                                aTGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAAc                                                                                       <  1:4628033/150‑1 (MQ=255)

                                                                  gCACGTTCACTTCTCGATCTGGAAAGAAGGCAAGCCAACGTTTGCTGGTAACGAATATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTc                                                                                     <  1:7802975/150‑1 (MQ=255)

                                                                  gCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTc                                                                                     <  1:1297399/150‑1 (MQ=255)

                                                                  gCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTc                                                                                     >  1:8007672/1‑150 (MQ=255)

                                                                    aCGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGt                                                                                   <  1:9117315/150‑1 (MQ=255)

                                                                     cGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTa                                                                                  <  1:8131606/150‑1 (MQ=255)

                                                                        gCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTCTCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACac                                                                               >  2:2221201/1‑149 (MQ=255)

                                                                          atttctCGATCTGGAAAGAAGGCAAGCCAACCTTCGCTGGTAACGAATATGCCGGCCTGTCGGAAAACTGCCTTTACTTCATCGGTGGCGTCATCAAGCACGCCAAGGCCGTGAATGCCTTCACCAACCCGTCGACCAACTCTTACAAGc                                                                             >  1:4428095/6‑150 (MQ=255)

                                                                          acaTGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGc                                                                             <  2:7383579/150‑1 (MQ=255)

                                                                          acaTGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGc                                                                             >  2:2676344/1‑150 (MQ=255)

                                                                          acaTGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGc                                                                             <  2:6378434/150‑1 (MQ=255)

                                                                            ttctCGATCTGGAAAGAAGGCAAGCCAACGTTTGCTGGTAACGAATATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGt                                                                           >  1:5345173/4‑150 (MQ=255)

                                                                            ttctCGATCTGGAAAGAAGGCAAGCCAACGTTTGCTGGTAACGAATATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGt                                                                           >  2:4643576/4‑150 (MQ=255)

                                                                            ttctCGATCTGGAAAGAAGGCAAGCCAACGTTTGCTGGTAACGAATATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGt                                                                           >  1:6062385/4‑150 (MQ=255)

                                                                            aTGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGt                                                                           >  1:639899/1‑150 (MQ=255)

                                                                             tctCGATCTGGAAAGAAGGCAAGCCAACGTTTGCTGGTAACGAATATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTc                                                                          <  2:7767449/148‑1 (MQ=255)

                                                                             tctCGATCTGGAAAGAAGGCAAGCCAACGTTTGCTGGTAACGAATATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTc                                                                          <  2:8009576/148‑1 (MQ=255)

                                                                             tGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTc                                                                          <  1:3358148/150‑1 (MQ=255)

                                                                             tGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTc                                                                          <  1:5128402/150‑1 (MQ=255)

                                                                              gTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCt                                                                         >  1:5631167/1‑150 (MQ=255)

                                                                              gTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCt                                                                         <  2:4913690/150‑1 (MQ=255)

                                                                                cGATCTGGAAAGAAGGCAAGCCAACGTTTGCTGGTAACGAATATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTCTgg                                                                       >  1:4355239/1‑150 (MQ=255)

                                                                                  aTCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTc                                                                     <  2:7021912/150‑1 (MQ=255)

                                                                                     tGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCa                                                                  >  2:6141277/1‑150 (MQ=255)

                                                                                      ggAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAg                                                                 <  2:5812294/150‑1 (MQ=255)

                                                                                      ggAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAg                                                                 <  2:9014209/150‑1 (MQ=255)

                                                                                      ggAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAg                                                                 <  1:5068510/150‑1 (MQ=255)

                                                                                       gAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAgg                                                                >  2:179831/1‑150 (MQ=255)

                                                                                         aaGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCt                                                              >  2:5356468/1‑150 (MQ=255)

                                                                                              ggCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGaa                                                         >  1:1812360/1‑150 (MQ=255)

                                                                                              ggCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGaa                                                         >  1:8579316/1‑150 (MQ=255)

                                                                                              ggCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGaa                                                         >  1:8806052/1‑150 (MQ=255)

                                                                                               gCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAg                                                        >  2:3346210/1‑150 (MQ=255)

                                                                                                 aaGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGcc                                                      <  2:6419893/150‑1 (MQ=255)

                                                                                                      cacCTTCTCGGGTGAAGGCTATTCCGGCCTGTCCGACACCGCCCTGTACTTCATcggc                                                                                                                                             <  1:5171900/58‑1 (MQ=255)

                                                                                                      cacCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGt                                                 <  1:7519632/150‑1 (MQ=255)

                                                                                                       acCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTa                                                >  1:7015415/1‑150 (MQ=255)

                                                                                                       acCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTa                                                >  2:2852887/1‑150 (MQ=255)

                                                                                                           tctcGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTACACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGc                                            <  2:2421684/150‑1 (MQ=255)

                                                                                                            ctcGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCt                                           <  1:4953638/150‑1 (MQ=255)

                                                                                                             tcGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTg                                          <  1:6226326/150‑1 (MQ=255)

                                                                                                                 gTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCt                                      >  2:4370903/1‑150 (MQ=255)

                                                                                                                  taacgaatATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTCTGGTCCCAGGTTATGAAGCTCCGGTTCTGCTGGCCTa                                     >  2:5069032/8‑150 (MQ=255)

                                                                                                                  tGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTa                                     >  2:2618073/1‑150 (MQ=255)

                                                                                                                   gAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTAc                                    >  1:19972/1‑150 (MQ=255)

                                                                                                                      gaatATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTCTGGTCCCAGGTTATGAAGCTCCGGTTCTGCTGGCCTATTCg                                 >  2:423779/4‑150 (MQ=255)

                                                                                                                       gCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCgg                                >  2:3654790/1‑150 (MQ=255)

                                                                                                                         tATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTCTGGTCCCAGGTTATGAAGCTCCGGTTCTGCTGGCCTATTCGGCt                              >  2:380102/1‑150 (MQ=255)

                                                                                                                         tATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCt                              >  2:8057390/1‑150 (MQ=255)

                                                                                                                          aTGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTc                             <  2:3866797/150‑1 (MQ=255)

                                                                                                                            gCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGc                           >  2:8408402/1‑150 (MQ=255)

                                                                                                                              cGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCaa                         <  2:4710572/150‑1 (MQ=255)

                                                                                                                                   tGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGtt                    >  1:2851038/1‑150 (MQ=255)

                                                                                                                                        gACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGcc               >  1:4256716/1‑150 (MQ=255)

                                                                                                                                          cacCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTc             <  2:6673800/150‑1 (MQ=255)

                                                                                                                                          cacCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTc             >  1:3672968/1‑150 (MQ=255)

                                                                                                                                          cacCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTc             <  2:4501944/150‑1 (MQ=255)

                                                                                                                                          cacCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTc             <  1:1754754/150‑1 (MQ=255)

                                                                                                                                            ccGCCCCGCACTCCATCGGCGCTATCATCAAGCACGCTACGGCCCTGAACGGCGTCCCCACCCCGCCGACCACCACGTCCAAGCGTCTGGCCCCAGGCTTCGAAGCCCCGGGAATGCGGGCCTACTCGGCTCGCAACCGGTCCGCCTCGa           <  2:559471/150‑1 (MQ=255)

                                                                                                                                                   gTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTAt    <  1:3211661/150‑1 (MQ=255)

                                                                                                                                                    tACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTAtt   <  2:821043/150‑1 (MQ=255)

CGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTC  >  NC_002947/5754748‑5755046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`