Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 445,110 T→G 18.9% intergenic (+80/‑208) PP_0367 → / → PP_0368 hypothetical protein/acyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947445,1100TG18.9% 410.0 / 80.6 178intergenic (+80/‑208)PP_0367/PP_0368hypothetical protein/acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (76/68);  new base G (8/26);  total (84/94)
Fisher's exact test for biased strand distribution p-value = 2.21e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 201 total aligned reads displayed.

CGAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACA‑ACAATTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAAACAACTGTT  >  NC_002947/444961‑445259
                                                                                                                                                       |                                                                                                                                                      
cGAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACA‑ACAAtt                                                                                                                                                        <  2:4803577/150‑1 (MQ=255)
cGAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACA‑ACAAtt                                                                                                                                                        <  1:9126814/150‑1 (MQ=255)
  aGCTCGGCGCACAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACTCCTACTCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGTCTTGCCCGCG‑AATGCGTCCGATCAGACA‑ACAAtttt                                                                                                                                                      <  2:6943357/150‑1 (MQ=255)
  aGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACA‑ACAAtttt                                                                                                                                                      <  2:8428280/150‑1 (MQ=255)
  aGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACA‑ACAAtttt                                                                                                                                                      <  2:6496386/150‑1 (MQ=255)
  aGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACA‑ACAAtttt                                                                                                                                                      <  1:6378340/150‑1 (MQ=255)
  aGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACA‑ACAAtttt                                                                                                                                                      <  1:5632262/150‑1 (MQ=255)
  aGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACA‑ACAAtttt                                                                                                                                                      <  1:5238762/150‑1 (MQ=255)
   gCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGGGTGGCCGCG‑CAAGCGTCCCCTCAGACACCCAAtttt                                                                                                                                                      >  2:5093121/1‑150 (MQ=255)
     tCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACA‑ACAATTTTG‑Tc                                                                                                                                                  <  1:3579079/150‑1 (MQ=255)
       ggCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACA‑ACAATTTTG‑TCTg                                                                                                                                                <  2:3847882/150‑1 (MQ=255)
       ggCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCGTGCCCGCG‑AATGCGTCCGATCAGACA‑ACAATTTTG‑TCTg                                                                                                                                                >  2:7537879/1‑150 (MQ=255)
        gcgcAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACA‑ACAATTTTG‑TCTgg                                                                                                                                               <  2:8566935/150‑1 (MQ=255)
        gcgcAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACA‑AAATTGTTG‑TCTga                                                                                                                                               <  2:2390159/150‑2 (MQ=255)
        gcgcAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AAGAGGCCCGACCAGACA‑AAATTGTTG‑TCTga                                                                                                                                               <  2:386023/150‑2 (MQ=25)
          gcAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGACCAGACA‑AAATTGTTG‑TCTGATc                                                                                                                                             <  1:4890969/150‑1 (MQ=38)
          gcAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AAGAGGCCCGACCAGACA‑AAATTGTTG‑TCTGATc                                                                                                                                             <  2:6678256/150‑1 (MQ=25)
           cAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGACCAGACA‑AAATTGTTG‑TCTGATCg                                                                                                                                            <  2:9139516/150‑1 (MQ=38)
             aaGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACA‑ACAATTTTG‑TCTGGTCggg                                                                                                                                          <  2:5695189/150‑1 (MQ=255)
              aGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACA‑ACAATTTTG‑TCTGGTCGGGc                                                                                                                                         <  1:7717578/150‑1 (MQ=255)
              aGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACA‑ACAATTTTG‑TCTGGTCGGGc                                                                                                                                         <  1:8444266/150‑1 (MQ=255)
              aGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGACCAGACA‑AAATTGTTG‑TCTGATCggac                                                                                                                                         <  1:9028645/150‑3 (MQ=38)
              aGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGCCCGACCAGACA‑AAATTGTTG‑TCTGATCggac                                                                                                                                         <  1:784197/150‑3 (MQ=37)
                   gtcAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACA‑ACAATTTTG‑TCTGGTCGGGCCTCtt                                                                                                                                    >  2:8354663/1‑150 (MQ=255)
                      aaGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACA‑ACAATTTTG‑TCTgg                                                                                                                                               <  2:6700098/136‑1 (MQ=255)
                        ggCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCTCG‑AATGCGTCCGATCAGACA‑ACAATTTTG‑TCTGGTCGGGCCTCTTcgcgt                                                                                                                               <  2:2562934/150‑2 (MQ=255)
                        ggCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACA‑ACAATTTTG‑TCTGGTCGGGCCTCTTCGCgg                                                                                                                               <  2:3465918/150‑1 (MQ=255)
                        ggCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AAGAGGCCCGACCAGACA‑AAATTGTTG‑TCTGATCGGACGCATTCGCgg                                                                                                                               <  1:9099582/150‑1 (MQ=18)
                        ggCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AAGAGGCCCGACCAGACA‑AAATTGTTG‑TCTGATCGGACGCATTCGCgg                                                                                                                               <  1:7031319/150‑1 (MQ=18)
                        ggCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AAGAGGCCCGACCAGACA‑AAATTGTTG‑TCTGATCGGACGCATTCGCgg                                                                                                                               <  1:4546444/150‑1 (MQ=18)
                        ggCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AAGAGGCCCGACCAGACA‑AAATTGTTG‑TCTGATCGGACGCATTCGCgg                                                                                                                               <  1:2370291/150‑1 (MQ=18)
                        ggCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AAGAGGCCCGACCAGACA‑AAATTGTTG‑TCTGATCGGACGCATTCGCgg                                                                                                                               <  2:935717/150‑1 (MQ=18)
                         gCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACA‑ACAATTTTG‑TCTGGTCGGGCCTCTTCGCggg                                                                                                                              <  2:4886023/150‑1 (MQ=255)
                           aTCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACA‑ACAATTTTG‑TCTGGTCGGGCCTCTTCGCGGGCa                                                                                                                            <  2:7224138/150‑1 (MQ=255)
                              cgacgaAATGCTTGGCCGGATCTTCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACA‑ACAATTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGc                                                                                                                         <  1:41504/150‑1 (MQ=255)
                              cgacgaAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AAGAGGCCCGACCAGACA‑AAATTGTTG‑TCTGATCGGACGCATTCGCGGGCAAGc                                                                                                                         <  1:3619271/150‑1 (MQ=17)
                                  gaAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑GATGCGTCCCCTCACCCA‑ACAAGTTTG‑TCTGGGGGGGGCTCGTCGCGGGCAAGCCCGc                                                                                                                     >  2:8065725/1‑150 (MQ=255)
                                          tGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACA‑ACAAtttt                                                                                                                                                      >  2:1511749/1‑110 (MQ=255)
                                          tGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACA‑ACAAtttt                                                                                                                                                      <  1:1511749/110‑1 (MQ=255)
                                          tGGCCGGACCATCGACACCTACGCCTGAATCCTGGCTTCATCCCCTATTTCCGCGCCGGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGACCGATCAGACA‑ACAATTTTG‑TGTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACaa                                                                                                             <  2:7632886/150‑1 (MQ=37)
                                                   catcGACACCTCCGCCTTAATACTTGCGACATCCCCTATTTCAGCGCCTGCATTGTAGCTGCGGGCTTGCCCGCG‑AATGCGACCGATCAGACA‑ACAATTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTtgtg                                                                                                    <  2:8205146/150‑1 (MQ=38)
                                                     tcGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCGTGCCCCCG‑CAAGCGGCCCCTCAGACA‑ACAAAGTTT‑GCGGGGCGGGCCGCGTCGCGGGCCACCCCCCGCCAACAACGGGGTGtggc                                                                                                  >  2:3748259/1‑150 (MQ=255)
                                                       gACACCACCGCGTTAATAGTTGGGACAGCCCCTATTCCCGCGCGGGCTTTGTAGGCGGGGGGTGGCCGGCT‑ATGGGGCCCGATCACACA‑AAAATTTGG‑TGGGGTGGGGCCTCTGCGGGGCCACCCCCGCTCCTACAAGGGTTTGtggcat                                                                                                <  2:8730419/150‑1 (MQ=255)
                                                        acacCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACA‑ACAATTTTG‑TCTGGTCGGGc                                                                                                                                         >  1:286698/1‑108 (MQ=255)
                                                        acacCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACA‑ACAATTTTG‑TCTGGTCGGGc                                                                                                                                         <  2:286698/108‑1 (MQ=255)
                                                                    gAATACTTGCGACAGCCCCTTTTTCCGCGCCGGCCTTGTAGGTGGGGGCTTGCCCGCG‑ACTGCGTCCTAACAGACA‑ACAATTTGG‑TCTGGTCGGGCCTGTTCGCGGGCACGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGAtt                                                                                   <  2:6640801/150‑1 (MQ=255)
                                                                     atgcggggCGTCACCCCCTATGCCCGGCCCGGCGTTGTCGGCGGGGGGTGCCCCGCG‑ACGGCGCCCTCTCACACA‑ACACTTGTG‑TGTGGCGGGGCCGCGGGGGGGGCACGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGAttt                                                                                  <  2:7900112/143‑1 (MQ=255)
                                                                                    cccTATTTCAGCGCCTGCCTTGTAGGAGCGGGGGTGCCCCCC‑AATTCGGGCGCTCAGACA‑AAAAATTTT‑TGTGGTCGGGGCGCGTCCCGGGCCATCCCCCACCAACAATGGTTTTTGGCATGGCATGAAAGATTTTCCAATTTTGGTGGc                                                                   >  2:1414537/1‑150 (MQ=255)
                                                                                     ccTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCC‑AAATCGTCCCATCAGTCA‑ACAAATTTG‑TCTGGGAGGGCCGCGTGGCGGGCAAGCCCCCTCCGACAAGGGTGTTTGGCAAGGCATGAATGATTTGGCAATTTTAGTGGCg                                                                  >  2:4554678/1‑150 (MQ=255)
                                                                                     ccTATTTCAGCGCCTGCCTTGTAGGAGCGGGCGTGCCCCCG‑AATGCGGACGCTCCCACA‑ACAAATTTT‑TGTGGGGGGGCCTCGTCGCGGGCCAGCCCCCTCCCACAAAGGGGTGTGGGCTGGCATGTCTGAATTGCCAATTTTCCTGGCg                                                                  >  2:3649191/1‑150 (MQ=255)
                                                                                          ttCCGCGCCGGCCTTGTCGGCGGGGGCTGCCCCGCC‑ACTGCGCCCGAACACACA‑A‑AATTTTGTTCGGGTCGGGCCTCTCCGGGGCCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAAt                                                             <  2:3296616/150‑1 (MQ=255)
                                                                                                   gcctttgttgGCGCGGGCGGCCCCGCG‑ACTGCGTCCCAACCAACA‑CCATTTTGG‑GGTGGTGGGGCCTCCCCGCGGGCACCCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATa                                                    <  1:7537879/146‑1 (MQ=255)
                                                                                                           tggAGCGGGCTTGCGCTCT‑AAGGCTTCCTCTCCTCAA‑ACATTTTGG‑TCGGGGGGGGCCTCTTGGCGGGAATGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATcc                                            <  2:1968839/149‑1 (MQ=255)
                                                                                                             gcgggggCTGGCCCGCC‑ACTGCGCCCCACCACACA‑ACAATTGTG‑TGGGGGCGGCCCTCTCCGCGGGCACCCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCtt                                          <  1:6742231/146‑1 (MQ=255)
                                                                                                              gcgggggTTGCCCGGGTATTGCGTCCTATCACACA‑AAATTTTTG‑TGGGGCGGGGCCTCTGCGCGGCCACGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCtt                                          <  1:2986595/147‑1 (MQ=255)
                                                                                                              aGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACA‑ACAATTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTACCTCCTTg                                         >  2:5885032/1‑150 (MQ=255)
                                                                                                               gCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACA‑ACAATTTTT‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTTCCATTTTTCGTGGCGTAAATTGCACCATAGTCCATCATTGa                                        >  1:7998886/1‑150 (MQ=255)
                                                                                                                     ttGCCCGCG‑AATGCGTCCGATCAGACA‑ACAATTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGaaa                                  >  1:7281357/1‑150 (MQ=255)
                                                                                                                        cccGCG‑AAGAGGCCCGACCAGACA‑AAATTGTTG‑TCTGATCGGACGCATTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCt                               >  1:5512363/1‑150 (MQ=18)
                                                                                                                        cccGCG‑AAGAGGCCCGACCAGACA‑AAATTGTTG‑TCTGATCGGACGCATTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCt                               >  2:2994741/1‑150 (MQ=18)
                                                                                                                         ccGCG‑AATGCGTCCGATCAGACA‑ACAATTTTG‑TCTGGTCGGGCCTCTTCGGGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTg                              >  1:4993116/1‑150 (MQ=255)
                                                                                                                         ccGCG‑AATGCGTCCGATCAGACA‑ACAATTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACaa                                                                                                             >  2:7185886/1‑71 (MQ=255)
                                                                                                                         ccGCG‑AATGCGTCCGATCAGACA‑ACAATTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGTCGTAAAt                                                             <  1:7172161/119‑1 (MQ=255)
                                                                                                                         ccGCG‑AATGCGTCCGATCAGACA‑ACAATTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGTCGTAAAt                                                             >  2:7172161/1‑119 (MQ=255)
                                                                                                                         ccGCG‑AATGCGTCCGATCAGACA‑ACAATTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTg                              >  2:3094776/1‑150 (MQ=255)
                                                                                                                         ccGCG‑AATGCGTCCGATCAGACA‑ACAATTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTg                              >  1:6503236/1‑150 (MQ=255)
                                                                                                                         ccGCG‑AATGCGTCCGATCAGACA‑ACAATTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTg                              >  1:1901012/1‑150 (MQ=255)
                                                                                                                         ccGCG‑AATGCGTCCGATCAGACA‑ACAATTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTg                              >  2:6175340/1‑150 (MQ=255)
                                                                                                                         ccGCG‑AATGCGTCCGATCAGACA‑ACAATTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTg                              >  1:444634/1‑150 (MQ=255)
                                                                                                                                    gTCCGATCAGACA‑ACAATTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTaa                        >  2:1601660/1‑146 (MQ=255)
                                                                                                                                    gTCCGATCAGACA‑ACAATTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGt                    >  2:400243/1‑150 (MQ=255)
                                                                                                                                     tCCGATCAGACA‑ACAATTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTa                   >  1:7493975/1‑150 (MQ=255)
                                                                                                                                      ccGATCAGACA‑ACAATTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGa                             >  2:1263837/1‑139 (MQ=255)
                                                                                                                                      ccGATCAGACA‑ACAATTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGa                             <  1:1263837/139‑1 (MQ=255)
                                                                                                                                      ccGACCAGACA‑AAATTGTTG‑TCTGATCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGAttt                                                                                  <  1:7055673/86‑1 (MQ=18)
                                                                                                                                      ccGACCAGACA‑AAATTGTTG‑TCTGATCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGAttt                                                                                  >  2:7055818/1‑86 (MQ=18)
                                                                                                                                       cGATCAGACA‑ACAATTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAg                 >  2:1011676/1‑150 (MQ=255)
                                                                                                                                       cGACCAGACA‑AAATTGTTG‑TCTGATCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAg                 >  1:7225067/1‑150 (MQ=38)
                                                                                                                                         aTCAGACA‑ACAATTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGtt               >  1:5121193/1‑150 (MQ=255)
                                                                                                                                          tCAGACA‑ACAATTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGttt              >  2:4988145/1‑150 (MQ=255)
                                                                                                                                           cAGACA‑AAATTGTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTc             >  2:5219736/1‑150 (MQ=255)
                                                                                                                                           accaca‑acaTTTTGG‑TCTGGGCGCGCCACTCCGGGGCCACCCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTc             <  1:6807959/147‑1 (MQ=255)
                                                                                                                                             gACA‑ACAATTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCaa           >  1:6678504/1‑150 (MQ=255)
                                                                                                                                             gACA‑ACAATTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCaa           >  1:1677932/1‑150 (MQ=255)
                                                                                                                                                a‑acaaTTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAaaca        >  2:7286965/1‑150 (MQ=255)
                                                                                                                                                a‑acaaTTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAaaca        >  2:7732837/1‑150 (MQ=255)
                                                                                                                                                a‑acaaTTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTAGACAGAAAGCTGAGCTAAACGTAAGTTTCAaaca        >  2:7671976/1‑150 (MQ=255)
                                                                                                                                                a‑acaaTTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGAAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAaaca        >  2:7672240/1‑150 (MQ=255)
                                                                                                                                                a‑acaaTTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGAGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAaaca        >  1:5587022/1‑150 (MQ=255)
                                                                                                                                                  acatTTTGG‑TGTGGTGGGGCCTCTGCGCGGGAACGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAaacaa       <  1:8139219/150‑1 (MQ=255)
                                                                                                                                                  acaaTTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAaacaa       >  1:808252/1‑150 (MQ=255)
                                                                                                                                                  aaatttttG‑TAGGGTCGGGCCTCCCCGGGGCCAACCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGATAGCTGAGCTAAACGTAAGTTTCAaacaa       <  1:3649191/146‑1 (MQ=255)
                                                                                                                                                   caaTTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAaacaac      >  1:5812002/1‑150 (MQ=255)
                                                                                                                                                    aaTTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAAACAACt     >  2:8170980/1‑150 (MQ=255)
                                                                                                                                                    aaTTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAAACAACt     >  1:7083304/1‑150 (MQ=255)
                                                                                                                                                     aTTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAAACAACTg    >  2:9091468/1‑150 (MQ=255)
                                                                                                                                                      ttttG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAAACAACTGt   >  2:20738/1‑150 (MQ=255)
                                                                                                                                                      ttttG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAAACAACGGt   >  2:7853607/1‑150 (MQ=255)
                                                                                                                                                       tttG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAAACAACTGtt  <  2:6768158/150‑1 (MQ=255)
                                                                                                                                                       tttG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAAACAACTGtt  >  1:1407600/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                      
CGAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACA‑ACAATTTTG‑TCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAAACAACTGTT  >  NC_002947/444961‑445259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑