BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,754,931	C→T	10.8%	A307A (GCC→GCT)	glnA →	glutamine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,754,931	0	C	T	10.8%	695.0 / 57.3	249	A307A (GCC→GCT)	glnA	glutamine synthetase
Reads supporting (aligned to +/- strand): ref base C (116/106); new base T (17/10); total (133/116)
Fisher's exact test for biased strand distribution p-value = 3.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

Only 100 of 258 total aligned reads displayed.

AAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGA  >  NC_002947/5754782‑5755080

     aCTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGaa                                                                                                                                                  >  1:5520121/1‑150 (MQ=255)

     aCTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGaa                                                                                                                                                  <  2:5046602/150‑1 (MQ=255)

      cTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAAc                                                                                                                                                 <  1:5890013/150‑1 (MQ=255)

         tACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGc                                                                                                                                              <  1:3959276/150‑1 (MQ=255)

           cGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCtt                                                                                                                                            <  2:2558774/150‑1 (MQ=255)

            ggCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTc                                                                                                                                           >  1:4992155/1‑150 (MQ=255)

            ggCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTc                                                                                                                                           >  1:9038993/1‑150 (MQ=255)

            ggCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTc                                                                                                                                           >  2:4922933/1‑150 (MQ=255)

             gCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCa                                                                                                                                          <  2:3413046/150‑1 (MQ=255)

             gCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCa                                                                                                                                          >  2:1754754/1‑150 (MQ=255)

              cGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCAc                                                                                                                                         <  2:8883328/150‑1 (MQ=255)

                aCAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCa                                                                                                                                       >  1:2876109/1‑150 (MQ=255)

                 cAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCaa                                                                                                                                      <  1:4053472/150‑1 (MQ=255)

                  aaCGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAAc                                                                                                                                     <  1:1203923/150‑1 (MQ=255)

                    cGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCagggt                                                                                                                                   <  2:7888740/150‑5 (MQ=255)

                    cGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAAccc                                                                                                                                   <  1:9163473/150‑1 (MQ=255)

                       cTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTc                                                                                                                                <  2:242929/150‑1 (MQ=255)

                        tCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCg                                                                                                                               >  2:8121459/1‑150 (MQ=255)

                                gCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTc                                                                                                                       <  1:1297399/150‑1 (MQ=255)

                                gCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTc                                                                                                                       <  2:3584255/150‑1 (MQ=255)

                                gCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTc                                                                                                                       >  2:6135711/1‑150 (MQ=255)

                                  aCGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGt                                                                                                                     <  1:9117315/150‑1 (MQ=255)

                                   cGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTa                                                                                                                    <  1:8131606/150‑1 (MQ=255)

                                      gCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACaa                                                                                                                 >  2:2212957/1‑150 (MQ=255)

                                        atttctCGATCTGGAAAGAAGGCAAGCCAACCTTCGCTGGTAACGAATATGCCGGCCTGTCGGAAAACTGCCTTTACTTCATCGGTGGCGTCATCAAGCACGCCAAGGCCGTGAATGCCTTCACCAACCCGTCGACCAACTCTTACAAGc                                                                                                               >  1:4428095/6‑150 (MQ=255)

                                        acaTGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGc                                                                                                               >  2:2676344/1‑150 (MQ=255)

                                        acaTGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGc                                                                                                               <  2:7383579/150‑1 (MQ=255)

                                          ttctCGATCTGGAAAGAAGGCAAGCCAACGTTTGCTGGTAACGAATATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGt                                                                                                             >  2:4643576/4‑150 (MQ=255)

                                          ttctCGATCTGGAAAGAAGGCAAGCCAACGTTTGCTGGTAACGAATATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGt                                                                                                             >  1:6062385/4‑150 (MQ=255)

                                          ttctCGATCTGGAAAGAAGGCAAGCCAACGTTTGCTGGTAACGAATATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGt                                                                                                             >  1:5345173/4‑150 (MQ=255)

                                          aTGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGt                                                                                                             <  1:3767038/150‑1 (MQ=255)

                                           tGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTc                                                                                                            <  2:7966092/150‑1 (MQ=255)

                                            gTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCt                                                                                                           <  2:4913712/150‑1 (MQ=255)

                                             tCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTg                                                                                                          <  1:8024644/150‑1 (MQ=255)

                                                aTCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTc                                                                                                       <  2:7021912/150‑1 (MQ=255)

                                                   tGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCa                                                                                                    >  2:3205507/1‑150 (MQ=255)

                                                   tGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCa                                                                                                    <  2:226433/150‑1 (MQ=255)

                                                     gAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAgg                                                                                                  >  2:179831/1‑150 (MQ=255)

                                                       aaGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCt                                                                                                >  1:5922298/1‑150 (MQ=255)

                                                       aaGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCt                                                                                                >  2:5356468/1‑150 (MQ=255)

                                                         gACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTc                                                                                              >  2:5954219/1‑150 (MQ=255)

                                                           cGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCATGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGATTCACCAACCCTTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGa                                                                                            <  2:6313624/150‑1 (MQ=255)

                                                            ggCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGaa                                                                                           <  1:8202996/150‑1 (MQ=255)

                                                            ggCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGaa                                                                                           >  1:8579316/1‑150 (MQ=255)

                                                            ggCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGaa                                                                                           >  1:5627071/1‑150 (MQ=255)

                                                             gCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAg                                                                                          >  2:3346210/1‑150 (MQ=255)

                                                               aaGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGcc                                                                                        <  2:6419893/150‑1 (MQ=255)

                                                                 gAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGcccc                                                                                      >  1:7947263/1‑150 (MQ=255)

                                                                    cacCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGt                                                                                   <  1:7519632/150‑1 (MQ=255)

                                                                     acCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTa                                                                                  >  2:7568892/1‑150 (MQ=255)

                                                                       cTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAAt                                                                                >  1:2826393/1‑150 (MQ=255)

                                                                       cTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAAt                                                                                <  1:5105179/150‑1 (MQ=255)

                                                                               gTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCt                                                                        >  2:4370903/1‑150 (MQ=255)

                                                                                 gAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTAc                                                                      >  1:3529399/1‑150 (MQ=255)

                                                                                 gAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTAc                                                                      >  1:19972/1‑150 (MQ=255)

                                                                                   aGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTc                                                                    <  1:3346210/150‑1 (MQ=255)

                                                                                    gaatATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTCTGGTCCCAGGTTATGAAGCTCCGGTTCTGCTGGCCTATTCg                                                                   >  2:423779/4‑150 (MQ=255)

                                                                                       tATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTCTGGTCCCAGGTTATGAAGCTCCGGTTCTGCTGGCCTATTCGGCt                                                                >  2:380102/1‑150 (MQ=255)

                                                                                        aTGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTc                                                               >  1:1452114/1‑150 (MQ=255)

                                                                                          gCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGc                                                             >  2:8408402/1‑150 (MQ=255)

                                                                                               ccTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCg                                                        <  2:9008368/150‑1 (MQ=255)

                                                                                                      gACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGcc                                                 >  1:3228622/1‑150 (MQ=255)

                                                                                                        cacCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTc                                               >  1:3672968/1‑150 (MQ=255)

                                                                                                        cacCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTc                                               <  2:6673684/150‑1 (MQ=255)

                                                                                                        cacCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTc                                               <  1:1754754/150‑1 (MQ=255)

                                                                                                        cacCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTc                                               <  2:4501944/150‑1 (MQ=255)

                                                                                                          ccGCCCCGCACTCCATCGGCGCTATCATCAAGCACGCTACGGCCCTGAACGGCGTCCCCACCCCGCCGACCACCACGTCCAAGCGTCTGGCCCCAGGCTTCGAAGCCCCGGGAATGCGGGCCTACTCGGCTCGCAACCGGTCCGCCTCGa                                             <  2:559471/150‑1 (MQ=255)

                                                                                                           cGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGAt                                            >  1:4727032/1‑150 (MQ=255)

                                                                                                             cccTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATcc                                          >  1:4523649/1‑150 (MQ=255)

                                                                                                             cccTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATcc                                          <  2:5530853/150‑1 (MQ=255)

                                                                                                             cccTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATcc                                          <  2:8027932/150‑1 (MQ=255)

                                                                                                             cccTGTACCTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATcc                                          <  2:859895/150‑1 (MQ=255)

                                                                                                               cTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGt                                        >  1:6371983/1‑150 (MQ=255)

                                                                                                                tGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGGCTGGTCCCAGGCTTCGACGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCGCGATCCGTa                                       <  2:7309628/150‑1 (MQ=255)

                                                                                                                     ttCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTCTGGTCCCAGGTTATGAAGCTCCGGTTCTGCTGGCCTATTCGGCTCGTAACCGTTCCGCTTCCTGCCGTATTcca                                  >  1:6838191/1‑149 (MQ=255)

                                                                                                                     ttCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTCTGGTCCCAGGTTATGAAGCTCCGGTTCTGCTGGCCTATTCGGCTCGTAACCGTTCCGCTTCCTGCCGTATTcca                                  >  1:6638074/1‑149 (MQ=255)

                                                                                                                      tCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCtt                                 <  2:2849829/150‑1 (MQ=255)

                                                                                                                      tCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCtt                                 <  1:3189802/150‑1 (MQ=255)

                                                                                                                       cATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTa                                <  2:4910214/150‑1 (MQ=255)

                                                                                                                       cATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTa                                <  2:401895/150‑1 (MQ=255)

                                                                                                                       cATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTa                                <  2:4112765/150‑1 (MQ=255)

                                                                                                                       cATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTCTGGTCCCAGGTTATGAAGCTCCGGTTCTGCTGGCCTATTCGGCTCGTAACCGTTCCGCTTCCTGCCGTATTccatt                                >  2:160265/1‑147 (MQ=255)

                                                                                                                         tCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACg                              >  1:5182357/1‑150 (MQ=255)

                                                                                                                          cggcggCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTCTGGTCCCAGGTTATGAAGCTCCGGTTCTGCTGGCCTATTCGGCTCGTAACCGTTCCGCTTCCTGCCGTATTccattcgg                             <  2:65509/150‑7 (MQ=255)

                                                                                                                           ggcggTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGtt                            >  1:1899260/1‑150 (MQ=255)

                                                                                                                               gTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCa                        <  2:9099853/150‑1 (MQ=255)

                                                                                                                                 atcatcAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGc                      >  1:7366151/1‑150 (MQ=255)

                                                                                                                                 atcatcAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGc                      <  1:2490324/150‑1 (MQ=255)

                                                                                                                                    atcaAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCg                   >  1:4325609/1‑150 (MQ=255)

                                                                                                                                      caAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGaa                 >  1:8903665/1‑150 (MQ=255)

                                                                                                                                      caAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGaa                 <  1:4923185/150‑1 (MQ=255)

                                                                                                                                       aaGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGaaa                <  1:7796225/150‑1 (MQ=255)

                                                                                                                                       aaGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTAGGCAGCCCGaaa                >  1:4346324/1‑150 (MQ=255)

                                                                                                                                        aGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAg               >  1:6770886/1‑150 (MQ=255)

                                                                                                                                        aGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAg               >  1:4182502/1‑150 (MQ=255)

                                                                                                                                           aCGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGccc            <  2:6721017/150‑1 (MQ=255)

                                                                                                                                           aCGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGccc            <  2:475105/150‑1 (MQ=255)

                                                                                                                                                    cccTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTTGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCg   >  2:3668288/1‑150 (MQ=255)

                                                                                                                                                    cccTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGGTCCGCCACGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCg   <  2:5236921/150‑1 (MQ=255)

                                                                                                                                                     ccTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGa  >  2:4591024/1‑150 (MQ=255)

AAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGA  >  NC_002947/5754782‑5755080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`