BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,754,985	C→T	6.7%	G325G (GGC→GGT)	glnA →	glutamine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,754,985	0	C	T	6.7%	785.9 / 29.9	253	G325G (GGC→GGT)	glnA	glutamine synthetase
Reads supporting (aligned to +/- strand): ref base C (140/96); new base T (12/5); total (152/101)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 254 total aligned reads displayed.

AAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCT  >  NC_002947/5754837‑5755134

aaGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCt                                                                                                                                                      >  1:5922298/1‑150 (MQ=255)

aaGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCt                                                                                                                                                      >  2:5356468/1‑150 (MQ=255)

          gAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGcccc                                                                                                                                            >  1:7947263/1‑150 (MQ=255)

              acCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTa                                                                                                                                        >  1:7015415/1‑150 (MQ=255)

              acCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTa                                                                                                                                        >  2:2852887/1‑150 (MQ=255)

              acCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTa                                                                                                                                        >  1:3167061/1‑150 (MQ=255)

                cTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAAt                                                                                                                                      <  1:5105179/150‑1 (MQ=255)

                cTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAAt                                                                                                                                      >  1:2826393/1‑150 (MQ=255)

                 ttCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATg                                                                                                                                     >  1:764913/1‑150 (MQ=255)

                  tctcGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTACACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGc                                                                                                                                    <  2:2421684/150‑1 (MQ=255)

                        gTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCt                                                                                                                              >  2:4370903/1‑150 (MQ=255)

                         taacgaatATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTCTGGTCCCAGGTTATGAAGCTCCGGTTCTGCTGGCCTa                                                                                                                             >  1:5080630/8‑150 (MQ=255)

                          gAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTAc                                                                                                                            >  1:3529399/1‑150 (MQ=255)

                             gaatATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTCTGGTCCCAGGTTATGAAGCTCCGGTTCTGCTGGCCTATTCg                                                                                                                         >  2:2745971/4‑150 (MQ=255)

                                tATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTCTGGTCCCAGGTTATGAAGCTCCGGTTCTGCTGGCCTATTCGGCt                                                                                                                      >  2:380102/1‑150 (MQ=255)

                                 aTGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTc                                                                                                                     >  1:1452114/1‑150 (MQ=255)

                                 aTGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTc                                                                                                                     <  2:3866797/150‑1 (MQ=255)

                                     cGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCaa                                                                                                                 <  2:4710572/150‑1 (MQ=255)

                                       gCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTCTGGTCCCAGGTTATGAAGCTCCGGTTCTGCTGGCCTATTCGGCTCGTAAcc                                                                                                               >  2:6381485/1‑150 (MQ=255)

                                              cGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGc                                                                                                        <  2:6004696/150‑1 (MQ=255)

                                               gACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGcc                                                                                                       >  1:4256716/1‑150 (MQ=255)

                                                 cacCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTc                                                                                                     <  2:6673684/150‑1 (MQ=255)

                                                 cacCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTc                                                                                                     <  2:4501944/150‑1 (MQ=255)

                                                  acCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCg                                                                                                    >  1:8737045/1‑150 (MQ=255)

                                                   ccGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGa                                                                                                   <  2:255166/150‑1 (MQ=255)

                                                      cccTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATcc                                                                                                <  2:8027932/150‑1 (MQ=255)

                                                      cccTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATcc                                                                                                >  1:4523649/1‑150 (MQ=255)

                                                         tGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTa                                                                                             >  1:2297764/1‑150 (MQ=255)

                                                          gTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTAt                                                                                            <  1:3211661/150‑1 (MQ=255)

                                                             cTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTcc                                                                                         <  2:4992155/150‑1 (MQ=255)

                                                             cTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTcc                                                                                         >  1:7209678/1‑150 (MQ=255)

                                                              ttCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCt                                                                                        <  1:5480932/150‑1 (MQ=255)

                                                              ttCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCt                                                                                        >  2:1658057/1‑150 (MQ=255)

                                                               tCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCtt                                                                                       <  1:8292336/150‑1 (MQ=255)

                                                               tCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTCTGGTCCCAGGTTATGAAGCTCCGGTTCTGCTGGCCTATTCGGCTCGTAACCGTTCCGCTTCCTGCCGTATTccat                                                                                       >  2:5533448/1‑148 (MQ=255)

                                                                cATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTa                                                                                      <  2:401895/150‑1 (MQ=255)

                                                                cATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTa                                                                                      <  2:4910378/150‑1 (MQ=255)

                                                                 aTCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTCTGGTCCCAGGTTATGAAGCTGCGGTTCTGCTGGCCTATTCGGCTCGTAACCGTTCCGCTTCCTGCCGTATTccattc                                                                                     <  1:8911705/150‑5 (MQ=255)

                                                                    ggcggTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGtt                                                                                  >  1:1899260/1‑150 (MQ=255)

                                                                     gcggTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTg                                                                                 >  2:5492174/1‑150 (MQ=255)

                                                                        gTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCa                                                                              <  2:9099853/150‑1 (MQ=255)

                                                                           tcatcaAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGcc                                                                           <  1:6710980/150‑1 (MQ=255)

                                                                             atcaAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCg                                                                         >  1:4325609/1‑150 (MQ=255)

                                                                              tcaAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGa                                                                        >  1:7484162/1‑150 (MQ=255)

                                                                              tcaAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGa                                                                        >  2:9003766/1‑150 (MQ=255)

                                                                               caAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGaa                                                                       <  1:4922933/150‑1 (MQ=255)

                                                                                aaGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGaaa                                                                      <  1:8121459/150‑1 (MQ=255)

                                                                                aaGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTAGGCAGCCCGaaa                                                                      >  1:4346324/1‑150 (MQ=255)

                                                                                 aGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAg                                                                     >  1:6770886/1‑150 (MQ=255)

                                                                                  gCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGc                                                                    >  2:8645583/1‑150 (MQ=255)

                                                                                   cACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGcc                                                                   >  1:982774/1‑150 (MQ=255)

                                                                                    aCGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGccc                                                                  <  2:6721017/150‑1 (MQ=255)

                                                                                     cGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCg                                                                 <  2:7489135/150‑1 (MQ=255)

                                                                                             cccTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTTGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCg                                                         >  2:3668288/1‑150 (MQ=255)

                                                                                             cccTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCg                                                         >  1:4012611/1‑150 (MQ=255)

                                                                                               cTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGaa                                                       <  1:8146257/150‑1 (MQ=255)

                                                                                               cTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGaa                                                       <  1:5359239/150‑1 (MQ=255)

                                                                                               cTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGaa                                                       <  1:8075651/150‑1 (MQ=255)

                                                                                                 gAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGc                                                     <  1:3699954/150‑1 (MQ=255)

                                                                                                     ggCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGACTACTCGGCTCGCAACCGTTCCGACTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGAACGc                                                 >  2:6478610/1‑150 (MQ=255)

                                                                                                       cTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCtt                                               >  1:4771738/1‑150 (MQ=255)

                                                                                                       cTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCtt                                               <  2:3344512/150‑1 (MQ=255)

                                                                                                        ttCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTc                                              >  1:7814980/1‑150 (MQ=255)

                                                                                                        ttCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTc                                              >  2:3107475/1‑150 (MQ=255)

                                                                                                        ttCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTc                                              >  2:3284657/1‑150 (MQ=255)

                                                                                                          cACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTccc                                            >  2:7450534/1‑150 (MQ=255)

                                                                                                           aCCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCg                                           >  1:3657465/1‑150 (MQ=255)

                                                                                                           aCCAACCCGTCGACCAACTCCTATAAGCGTCTGGTCCCAGGTTATGAAGCTCCGGTTCTGCTGGCCTATTCGGCTc                                                                                                                     >  2:7024423/1‑76 (MQ=255)

                                                                                                            ccAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCgg                                          >  2:2324121/1‑150 (MQ=255)

                                                                                                             cAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGa                                         >  1:3022046/1‑150 (MQ=255)

                                                                                                             cAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGa                                         >  1:8571716/1‑150 (MQ=255)

                                                                                                                cccGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGAccc                                      <  1:6135711/150‑1 (MQ=255)

                                                                                                                    tCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCg                                  <  1:179831/150‑1 (MQ=255)

                                                                                                                      gACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGc                                <  2:3699503/150‑1 (MQ=255)

                                                                                                                      gACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGc                                >  1:2031846/1‑150 (MQ=255)

                                                                                                                        ccAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCa                              >  2:8887113/1‑150 (MQ=255)

                                                                                                                        ccAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCa                              >  2:3543731/1‑150 (MQ=255)

                                                                                                                         cAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGGATCGAAGCACGCTTCCCGGACCCATCGGCCaa                             >  2:6237182/1‑150 (MQ=255)

                                                                                                                          aaCTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGACAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAAc                            <  1:2618073/150‑1 (MQ=255)

                                                                                                                           aCTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAAcc                           >  1:7450616/1‑150 (MQ=255)

                                                                                                                            cTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAAccc                          >  2:8103875/1‑150 (MQ=255)

                                                                                                                               gTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTa                       >  2:2045795/1‑150 (MQ=255)

                                                                                                                               gTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTa                       >  1:4644020/1‑150 (MQ=255)

                                                                                                                               gTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTa                       >  1:1607126/1‑150 (MQ=255)

                                                                                                                                tACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTAc                      <  2:8579316/150‑1 (MQ=255)

                                                                                                                                tACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTAc                      >  2:1567841/1‑150 (MQ=255)

                                                                                                                                   aaGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTg                   >  2:4156100/1‑150 (MQ=255)

                                                                                                                                     gCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGc                 <  2:5922298/150‑1 (MQ=255)

                                                                                                                                     gCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGc                 <  2:1812360/150‑1 (MQ=255)

                                                                                                                                        tCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGtt              >  1:7054933/1‑150 (MQ=255)

                                                                                                                                          tGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTcg            >  2:2037434/1‑150 (MQ=255)

                                                                                                                                          tGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTcg            >  2:4225446/1‑150 (MQ=255)

                                                                                                                                             tCCCAGGCTTCGAAGCCCCGGTCATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCgg         <  1:6237182/150‑1 (MQ=255)

                                                                                                                                               ccAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGcc       >  1:14121/1‑150 (MQ=255)

                                                                                                                                                 aGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCct     >  1:6573915/1‑150 (MQ=255)

                                                                                                                                                 aGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCct     >  2:8337601/1‑150 (MQ=255)

                                                                                                                                                 aGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCct     >  2:835621/1‑150 (MQ=255)

                                                                                                                                                 aGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCct     >  1:4056021/1‑150 (MQ=255)

                                                                                                                                                 aGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCct     >  1:1095383/1‑150 (MQ=255)

                                                                                                                                                   gCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATACCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCctgc   >  1:3327401/1‑150 (MQ=255)

AAGACGGCAAGAACACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCT  >  NC_002947/5754837‑5755134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`