BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,755,000	A→T	6.4%	V330V (GTA→GTT)	glnA →	glutamine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,755,000	0	A	T	6.4%	871.0 / 33.3	267	V330V (GTA→GTT)	glnA	glutamine synthetase
Reads supporting (aligned to +/- strand): ref base A (142/106); new base T (12/5); total (155/112)
Fisher's exact test for biased strand distribution p-value = 3.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 275 total aligned reads displayed.

ACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGA  >  NC_002947/5754851‑5755149

acCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTa                                                                                                                                                       >  2:7568892/1‑150 (MQ=255)

acCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTa                                                                                                                                                       >  1:3167061/1‑150 (MQ=255)

     ctcGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCt                                                                                                                                                  <  1:4953638/150‑1 (MQ=255)

           taacgaatATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTCTGGTCCCAGGTTATGAAGCTCCGGTTCTGCTGGCCTa                                                                                                                                            <  2:178271/143‑1 (MQ=255)

           tGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTa                                                                                                                                            >  2:2618073/1‑150 (MQ=255)

               gaatATGCCGGCCTGTCGGAAAACTGCCTGTTCTTCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTCTGGTCCCAGGTTATGAAGCTCCGGTTCTGCTGGCCTATTCg                                                                                                                                        >  2:6274571/4‑150 (MQ=255)

                  tATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCt                                                                                                                                     >  1:524015/1‑150 (MQ=255)

                   aTGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTc                                                                                                                                    <  1:6643521/150‑1 (MQ=255)

                            tGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGtt                                                                                                                           >  1:2851038/1‑150 (MQ=255)

                                cGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGc                                                                                                                       <  2:6004696/150‑1 (MQ=255)

                                 gACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGcc                                                                                                                      >  2:2336533/1‑150 (MQ=255)

                                   cacCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTc                                                                                                                    <  1:1754754/150‑1 (MQ=255)

                                   cacCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTc                                                                                                                    <  2:6673800/150‑1 (MQ=255)

                                    acCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCg                                                                                                                   >  1:8737045/1‑150 (MQ=255)

                                    acCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCg                                                                                                                   >  2:4665874/1‑150 (MQ=255)

                                        cccTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATcc                                                                                                               >  1:4523649/1‑150 (MQ=255)

                                          cTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGt                                                                                                             >  1:6371983/1‑150 (MQ=255)

                                            gTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTAt                                                                                                           <  1:3211661/150‑1 (MQ=255)

                                                ttCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCt                                                                                                       >  2:4920924/1‑150 (MQ=255)

                                                ttCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCt                                                                                                       <  1:5480932/150‑1 (MQ=255)

                                                 tCATCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTCTGGTCCCAGGTTATGAAGCTCCGGTTCTGCTGGCCTATTCGGCTCGTAACCGTTCCGCTTCCTGCCGTATTccat                                                                                                      >  2:5533448/1‑148 (MQ=255)

                                                  cATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTa                                                                                                     <  2:4113351/150‑1 (MQ=255)

                                                  cATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTa                                                                                                     <  1:6771977/150‑1 (MQ=255)

                                                  cATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTa                                                                                                     <  2:4910214/150‑1 (MQ=255)

                                                   aTCGGCGGCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTCTGGTCCCAGGTTATGAAGCTGCGGTTCTGCTGGCCTATTCGGCTCGTAACCGTTCCGCTTCCTGCCGTATTccattc                                                                                                    <  1:8911705/150‑5 (MQ=255)

                                                     cggcggCGTCATCAAGCACGCCAAGGCTGTGAACGCATTCACCAACCCGTCGACCAACTCCTATAAGCGTCTGGTCCCAGGTTATGAAGCTCCGGTTCTGCTGGCCTATTCGGCTCGTAACCGTTCCGCTTCCTGCCGTATTccattcgg                                                                                                  <  2:65509/150‑7 (MQ=255)

                                                      ggcggTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGtt                                                                                                 >  1:1899260/1‑150 (MQ=255)

                                                       gcggTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTg                                                                                                >  2:5492174/1‑150 (MQ=255)

                                                          gTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCa                                                                                             <  2:9099853/150‑1 (MQ=255)

                                                            atcatcAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGc                                                                                           >  1:1442913/1‑150 (MQ=255)

                                                               atcaAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCg                                                                                        >  1:4325609/1‑150 (MQ=255)

                                                                tcaAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGa                                                                                       >  2:9003766/1‑150 (MQ=255)

                                                                tcaAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGa                                                                                       >  1:3587292/1‑150 (MQ=255)

                                                                 caAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGaa                                                                                      >  2:6774176/1‑150 (MQ=255)

                                                                  aaGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGaaa                                                                                     <  1:8121459/150‑1 (MQ=255)

                                                                  aaGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTAGGCAGCCCGaaa                                                                                     >  1:4346324/1‑150 (MQ=255)

                                                                    gCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGc                                                                                   >  2:8645583/1‑150 (MQ=255)

                                                                               cccTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTTGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCg                                                                        >  2:3668288/1‑150 (MQ=255)

                                                                               cccTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCg                                                                        >  1:4012611/1‑150 (MQ=255)

                                                                               cccTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGGTCCGCCACGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCg                                                                        <  2:5236921/150‑1 (MQ=255)

                                                                                ccTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGa                                                                       >  2:7332103/1‑150 (MQ=255)

                                                                                ccTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGa                                                                       >  2:8850653/1‑150 (MQ=255)

                                                                                 cTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGaa                                                                      >  1:6744452/1‑150 (MQ=255)

                                                                                 cTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGaa                                                                      <  1:8075651/150‑1 (MQ=255)

                                                                                       ggCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGACTACTCGGCTCGCAACCGTTCCGACTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGAACGc                                                                >  2:6478610/1‑150 (MQ=255)

                                                                                         cTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCtt                                                              >  1:4771738/1‑150 (MQ=255)

                                                                                         cTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCtt                                                              <  1:5098970/150‑1 (MQ=255)

                                                                                          ttCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTc                                                             >  2:2040192/1‑150 (MQ=255)

                                                                                          ttCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTc                                                             >  2:3284657/1‑150 (MQ=255)

                                                                                          ttCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTc                                                             >  1:7968110/1‑150 (MQ=255)

                                                                                          ttCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTc                                                             >  1:7814980/1‑150 (MQ=255)

                                                                                           tCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTcc                                                            <  1:8270193/150‑1 (MQ=255)

                                                                                            cACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTccc                                                           <  2:2214270/150‑1 (MQ=255)

                                                                                            cACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTccc                                                           >  2:7450534/1‑150 (MQ=255)

                                                                                             aCCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCg                                                          >  2:1944488/1‑150 (MQ=255)

                                                                                             aCCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCg                                                          >  2:2494410/1‑150 (MQ=255)

                                                                                             aCCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCg                                                          >  1:3657465/1‑150 (MQ=255)

                                                                                             aCCAACCCGTCGACCAACTCCTATAAGCGTCTGGTCCCAGGTTATGAAGCTCCGGTTCTGCTGGCCTATTCGGCTc                                                                                                                                    <  1:7024423/76‑1 (MQ=255)

                                                                                              ccAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCgg                                                         >  1:7127902/1‑150 (MQ=255)

                                                                                               cAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGa                                                        >  1:8571716/1‑150 (MQ=255)

                                                                                               cAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGa                                                        >  1:3022046/1‑150 (MQ=255)

                                                                                                  cccGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGAccc                                                     <  1:6135711/150‑1 (MQ=255)

                                                                                                     gTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATc                                                  <  2:1130704/150‑1 (MQ=255)

                                                                                                      tCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCg                                                 <  1:4591024/150‑1 (MQ=255)

                                                                                                        gACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGc                                               >  1:2031846/1‑150 (MQ=255)

                                                                                                            aaCTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAAc                                           <  2:2876109/150‑1 (MQ=255)

                                                                                                            aaCTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAAc                                           >  2:3746869/1‑150 (MQ=255)

                                                                                                              cTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAAccc                                         >  2:8103875/1‑150 (MQ=255)

                                                                                                               tCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCg                                        >  2:3909384/1‑150 (MQ=255)

                                                                                                                 gTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTa                                      >  2:3846828/1‑150 (MQ=255)

                                                                                                                  tACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTAc                                     <  2:8579316/150‑1 (MQ=255)

                                                                                                                       gCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGc                                <  2:1261258/150‑1 (MQ=255)

                                                                                                                         gTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGt                              >  2:3113717/1‑150 (MQ=255)

                                                                                                                            tGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTcg                           <  2:3529399/150‑1 (MQ=255)

                                                                                                                            tGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTcg                           >  2:4225446/1‑150 (MQ=255)

                                                                                                                                 ccAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGcc                      >  2:430445/1‑150 (MQ=255)

                                                                                                                                 ccAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGcc                      >  1:7427929/1‑150 (MQ=255)

                                                                                                                                 ccAGACTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGcc                      >  2:8245766/1‑150 (MQ=255)

                                                                                                                                   aGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCct                    >  2:835621/1‑150 (MQ=255)

                                                                                                                                   aGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCct                    >  1:1095383/1‑150 (MQ=255)

                                                                                                                                   aGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCct                    >  1:4056022/1‑150 (MQ=255)

                                                                                                                                     gCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATACCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCctgc                  >  1:3327401/1‑150 (MQ=255)

                                                                                                                                      cTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCctgct                 <  1:7568892/150‑1 (MQ=255)

                                                                                                                                       ttCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGTGTTCGCGGCCctgctg                >  1:1372440/1‑150 (MQ=255)

                                                                                                                                       ttCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCctgctg                <  2:5627071/150‑1 (MQ=255)

                                                                                                                                       ttCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCctgctg                >  1:6226169/1‑150 (MQ=255)

                                                                                                                                       ttCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCctgctg                >  1:1541821/1‑150 (MQ=255)

                                                                                                                                       ttCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCctgctg                <  1:2676344/150‑1 (MQ=255)

                                                                                                                                        tCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGa               <  1:7471834/150‑1 (MQ=255)

                                                                                                                                           aaGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGAtgt            <  1:2336533/150‑2 (MQ=255)

                                                                                                                                             gCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGAtggct          >  1:4232889/1‑150 (MQ=255)

                                                                                                                                             gCCCCGGTAACGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGAtggct          <  1:485209/150‑1 (MQ=255)

                                                                                                                                               cccGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGAtggctgg        >  2:7847833/1‑150 (MQ=255)

                                                                                                                                               cccGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGAtggctgg        >  2:1650504/1‑150 (MQ=255)

                                                                                                                                                ccGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGAtggctggc       >  1:3816595/1‑150 (MQ=255)

                                                                                                                                                ccGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGAtggctggc       >  1:8357345/1‑150 (MQ=255)

                                                                                                                                                    tAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTgg   <  2:2773890/150‑1 (MQ=255)

                                                                                                                                                    tAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTgg   >  1:2536722/1‑150 (MQ=255)

                                                                                                                                                     aaTGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGa  <  2:444267/150‑1 (MQ=255)

                                                                                                                                                     aaTGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTAGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGa  <  1:2212957/150‑1 (MQ=255)

ACCTTCTCGGGTGAAGGCTATGCCGGCCTGTCCGACACCGCCCTGTACTTCATCGGCGGTATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGTCGACCAACTCGTACAAGCGTCTGGTCCCAGGCTTCGAAGCCCCGGTAATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTACGTTGGCAGCCCGAAAGCCCGACGTATCGAAGCACGCTTCCCGGACCCATCGGCCAACCCGTACCTGGCGTTCGCGGCCCTGCTGATGGCTGGCCTGGA  >  NC_002947/5754851‑5755149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`