BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,970,399	T→G	8.8%	T51P (ACG→CCG)	PP_5723 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,970,399	0	T	G	8.8%	538.3 / 13.0	171	T51P (ACG→CCG)	PP_5723	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (33/120); new base G (14/4); total (47/124)
Fisher's exact test for biased strand distribution p-value = 3.57e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 175 total aligned reads displayed.

AAACAGCGCGTTATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCA  >  NC_002947/5970250‑5970548

 acaggcgcgaggcctggtcgctcagggcatcgagaagggctttgggcgcgagcatggggctgtccactgaaacggagaaatggccgccagtgtagcacgcacaaacctggcgcctgagtgcacattggcctttgtgtGCGGCCTTGCGTc                                                                                                                                                       >  2:1371249‑M1/138‑150 (MQ=255)

 aaCAGCGCGTTATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTc                                                                                                                                                       <  1:848313/150‑1 (MQ=255)

 aaCAGCGCGTTATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTc                                                                                                                                                       <  1:8535595/150‑1 (MQ=255)

  caggcgcgaggcctggtcgctcagggcatcgagaagggctttgggcgcgagcatggggctgtccactgaaacggagaaatggccgccagtgtagcacgcacaaacctggcgcctgagtgcacattggcctttgtgtGCGGCCTTGCGTcg                                                                                                                                                      >  2:5965310‑M1/137‑150 (MQ=255)

  caggcgcgaggcctggtcgctcagggcatcgagaagggctttgggcgcgagcatggggctgtccactgaaacggagaaatggccgccagtgtagcacgcacaaacctggcgcctgagtgcacattggcctttgtgtGCGGCCTTGCGTcg                                                                                                                                                      >  2:3328077‑M1/137‑150 (MQ=255)

  aCAGCGCGTTATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTcg                                                                                                                                                      <  1:8412483/150‑1 (MQ=255)

  aCAGCGCGTTATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTcg                                                                                                                                                      <  2:2282100/150‑1 (MQ=255)

   cAGCGCGTTATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTcgc                                                                                                                                                     <  1:2031184/150‑1 (MQ=255)

   cAGCGCGTTATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTcgc                                                                                                                                                     <  1:52248/150‑1 (MQ=255)

    aGCGCGTTATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGTGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTcgcg                                                                                                                                                    >  1:304914/1‑150 (MQ=255)

     gcgcgaggcctggtcgctcagggcatcgagaagggctttgggcgcgagcatggggctgtccactgaaacggagaaatggccgccagtgtagcacgcacaaacctggcgcctgagtgcacattggcctttgtgtGCGGCCTTGCGTCGCGa                                                                                                                                                   >  2:6220231‑M1/134‑150 (MQ=255)

     gcgcgaggcctggtcgctcagggcatcgagaagggctttgggcgcgagcatggggctgtccactgaaacggagaaatggccgccagtgtagcacgcacaaacctggcgcctgagtgcacattggcctttgtgtGCGGCCTTGCGTCGCGa                                                                                                                                                   >  2:1526114‑M1/134‑150 (MQ=255)

      cgcgaggcctggtcgctcagggcatcgagaagggctttgggcgcgagcatggggctgtccactgaaacggagaaatggccgccagtgtagcacgcacacacctggcgcctgagtgcacattggcctttgtgtGCGGCCTTGCGTCGCGAt                                                                                                                                                  >  2:75275‑M1/133‑150 (MQ=255)

       gcgaggcctggtcgctcagggcatcgagaagggctttgggcgcgagcatggggctgtccactgaaacggagaaatggccgccagtgtagcacgcacaaacctggcgcctgagtgcacattggcctttgtgtGCGGCCTTGCGTCGCGAtt                                                                                                                                                 >  1:1039666‑M1/132‑150 (MQ=255)

        cgtgaggtgAGTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTg                                                                                                                                                <  1:6810985/144‑1 (MQ=255)

        cgTTATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTg                                                                                                                                                <  2:1678556/150‑1 (MQ=255)

        cgTTATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTg                                                                                                                                                <  1:8622592/150‑1 (MQ=255)

        cgTTATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGGcgcgggtg                                                                                                                                                >  2:2882914/1‑146 (MQ=255)

         gaggcctggtcgctcagggcatcgagaagggctttgggcgcgagcatggggctgtccactgaaacggagaaatggccgccagtgtagcacgcacaaacctggcgcctgagtgcacattggcctttgtgtGCGGCCTTGCGTCGCGATTgg                                                                                                                                               >  1:6652592‑M1/130‑150 (MQ=255)

          ttATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTggg                                                                                                                                              <  2:7188352/150‑1 (MQ=255)

          ttATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTggg                                                                                                                                              <  2:8872083/150‑1 (MQ=255)

            aTGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGt                                                                                                                                            <  2:4583384/150‑1 (MQ=255)

             tgtgATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTgggggg                                                                                                                                           <  1:3677718/150‑3 (MQ=255)

              gtgATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGTGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGc                                                                                                                                          <  2:304914/150‑1 (MQ=255)

              gtgATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGc                                                                                                                                          <  1:7479683/150‑1 (MQ=255)

                  tttGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAg                                                                                                                                      >  2:5907925/1‑150 (MQ=255)

                  tcgctcagggcatcgagaagggctttgggcgcgagcatggggctgtccactgaaacggagaaatggccgccagtgtagcacgcacaaacctggcgcctgagtgcacattggcctttgtgtGCGGCCTTGGGTgggggttgggggggacgg                                                                                                                                      <  1:5900466‑M1/30‑8 (MQ=255)

                    gctcagggcatcgagaagggctttgggcgcgagcatggggctgtccactgaaacggagaaatggccgccagtgtagcacgcacaaacctggcgcctgagtgcacattggcctttgtgtGCGGCCTTgcggcgcgggtggggtgggccggg                                                                                                                                    <  1:7477772‑M1/32‑8 (MQ=255)

                     gCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAgcgc                                                                                                                                   <  2:218329/150‑1 (MQ=255)

                      cTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGTGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGcc                                                                                                                                  <  1:871432/150‑1 (MQ=255)

                      cTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGcc                                                                                                                                  <  1:871309/150‑1 (MQ=255)

                         gggATTGGCCAGTCGCCAGCTCGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCATCAGCTTGTAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGATGCGcccac                                                                                                                               <  2:1770150/150‑3 (MQ=255)

                           gATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGGGGCGCGGGTGGGGGGGGGAGCGccccccc                                                                                                                             >  1:2241783/1‑149 (MQ=255)

                            aTTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCaa                                                                                                                            <  2:319385/150‑1 (MQ=255)

                             ttGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAg                                                                                                                           <  1:6697335/150‑1 (MQ=255)

                             ttGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAg                                                                                                                           <  1:6697280/150‑1 (MQ=255)

                                gCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTTGGGGGCGGGTTGGGGGGGAAGCGCCCCCCCAGaat                                                                                                                        >  2:4159963/1‑148 (MQ=255)

                                 ccAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGAttt                                                                                                                       <  1:4006419/150‑1 (MQ=255)

                                 ccAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGAttt                                                                                                                       <  2:1741253/150‑1 (MQ=255)

                                 ccAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCGTGCGTGACGATTTGGGTGCGCAGCGCCCCCCCAGaagt                                                                                                                       >  1:7754742/1‑147 (MQ=255)

                                  cAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGGCGGGGGTGGGGGGCGCGGCGCCCCCCCAgggttg                                                                                                                      >  2:6027490/1‑145 (MQ=255)

                                   aGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTtgt                                                                                                                     <  2:7679370/150‑1 (MQ=255)

                                     tCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGc                                                                                                                   <  1:5299278/150‑1 (MQ=255)

                                     tCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGc                                                                                                                   <  2:3835398/150‑1 (MQ=255)

                                       gCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCt                                                                                                                 <  1:1645256/150‑1 (MQ=255)

                                       gCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGACAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCt                                                                                                                 <  2:7777186/150‑1 (MQ=255)

                                        ccAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCGTGGGGGGGGGGTTGGGGGGGGGGCGCCCCCCAACAATtgtggggg                                                                                                                >  2:2502323/1‑146 (MQ=255)

                                           gCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGGGGCGCGGGAGGGGGGCGGAGCGCCCCCCCAGAGTtttggcgtttt                                                                                                             >  2:5751070/1‑143 (MQ=255)

                                            cACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATg                                                                                                            <  2:9043242/150‑1 (MQ=255)

                                             aCGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGc                                                                                                           <  1:820430/150‑1 (MQ=255)

                                               gggAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTg                                                                                                         <  2:5208693/150‑1 (MQ=255)

                                               gggAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTg                                                                                                         <  2:440458/150‑1 (MQ=255)

                                               gggAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTg                                                                                                         <  2:4878119/150‑1 (MQ=255)

                                                ggAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGa                                                                                                        <  2:3952319/150‑1 (MQ=255)

                                                 gAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGGGGCGGGGTTTTGGTGGGGAGCGCCCCCCACCAATTTTGGCATGATGCTggt                                                                                                       >  2:3462704/1‑148 (MQ=255)

                                                 gAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGGCGCGTTTGGGGGGCGAAGCGCCCCCCCACCTGTGTGCCGTGATGCTGat                                                                                                       >  2:7477772/1‑150 (MQ=255)

                                                       tGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGcc                                                                                                <  1:3234955/150‑1 (MQ=255)

                                                         cacaATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCgg                                                                                              <  1:8730044/150‑1 (MQ=255)

                                                         cacaATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCgg                                                                                              <  1:1395543/150‑1 (MQ=255)

                                                          acaATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCggg                                                                                             <  1:5641411/150‑1 (MQ=255)

                                                           caATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCgggg                                                                                            <  2:9115211/150‑1 (MQ=255)

                                                            aaTTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGc                                                                                           <  2:5439568/150‑1 (MQ=255)

                                                              tttCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCg                                                                                         <  1:1044604/150‑1 (MQ=255)

                                                                tCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCt                                                                                       <  2:420454/150‑1 (MQ=255)

                                                                tCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCt                                                                                       <  1:9019623/150‑1 (MQ=255)

                                                                 cGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCtg                                                                                      <  2:7527880/150‑1 (MQ=255)

                                                                 cGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCtg                                                                                      <  1:2372537/150‑1 (MQ=255)

                                                                  ggTGCGCATCAGCGTGGCAAGCTGCGCGAAAAATGTTCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCtgt                                                                                     <  1:5807611/150‑1 (MQ=255)

                                                                  ggTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCtgt                                                                                     <  1:8194290/150‑1 (MQ=255)

                                                                    tGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGGCGCGCGTGGGGGGCGCCGCGCCCCCCCACAGTTGTGCCGTGTTGGTGAGAGTTGCCGGGGcgcggggg                                                                                    >  2:5900466/1‑143 (MQ=255)

                                                                     gcgcAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCa                                                                                  <  1:8110614/150‑1 (MQ=255)

                                                                       gcagcaGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGc                                                                                <  1:9119125/150‑1 (MQ=255)

                                                                        cagcagCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGcc                                                                               <  1:5470298/150‑1 (MQ=255)

                                                                           gagaaatggccgccagtgtagcacgcacaaacctggcgcctgagtgcacattggcctttgtgtGCGGCCTTGCGTCGCGATTGCGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCaa                                                                            >  2:1067361‑M1/64‑150 (MQ=255)

                                                                            agcGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAAt                                                                           <  2:5802639/150‑1 (MQ=255)

                                                                            agaaatggccgccagtgtagcacgcacaaacctggcgcctgagtgcacattggcctttgtgtGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAAt                                                                           >  2:1713339‑M1/63‑150 (MQ=255)

                                                                            agaaatggccgccagtgtagcacgcacaaacctggcgcctgagtgcacattggcctttgtgtGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAAt                                                                           >  2:6490043‑M1/63‑150 (MQ=255)

                                                                             gaaatggccgccagtgtagcacgcacaaacctggcgcctgagtgcacattggcctttgtgtGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATc                                                                          >  1:6102398‑M1/62‑150 (MQ=255)

                                                                               ggggCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATcgc                                                                        <  1:5324215/150‑1 (MQ=255)

                                                                                 ggCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGa                                                                      <  1:8996746/150‑1 (MQ=255)

                                                                                 ggCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGa                                                                      <  1:7851767/150‑1 (MQ=255)

                                                                                 ggCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGa                                                                      <  2:7185001/150‑1 (MQ=255)

                                                                                 ggCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGa                                                                      <  2:6273573/150‑1 (MQ=255)

                                                                                  ggccgccagtgtagcacgcacaaacctggcgcctgagtgcacattggcctttgtgtGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGAc                                                                     >  2:8440210‑M1/57‑150 (MQ=255)

                                                                                  gCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGAc                                                                     <  1:2003823/150‑1 (MQ=255)

                                                                                    aaGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGc                                                                   <  2:6066240/150‑1 (MQ=255)

                                                                                      gCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCaa                                                                 <  2:1187713/150‑1 (MQ=255)

                                                                                      gCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCaa                                                                 <  1:2940961/150‑1 (MQ=255)

                                                                                           gcgAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGGCGCGAGTGGGGTGCGCAGCCCCCCCCCACAATTTTTCCGTGATGCTGGTA‑TTGCCCGGGCCCCGGGGCAGCCCAAAAGCGCCGCAACGCCg                                                            >  2:8957847/1‑150 (MQ=255)

                                                                                             gAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCGTTCGTGGCGGGTTGGGTGCGGAGCGCCCCCCCAGATTTGTTGCGTGTTGGTGATA‑TTTGCGGGGGCGCGGTGGGGCCCCAAAGAGACGCCACGCCGca                                                          >  2:1422905/1‑150 (MQ=255)

                                                                                                        aaCAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGGGGGGCGGTTTGGGGGGGGAGCGCCCCCCAAGATTTTTGGCGTTATGGTGTTA‑ATGCCGGGGGCGCGGGGCAGCCCACTAGCGACGCAACGCCGCACACACAGGccc                                               >  2:5199059/1‑149 (MQ=255)

                                                                                                              ttGAAAACTGCACGCGATCTTTGTGGGAGCGGCCGTGCGGCGCCCTTGGGGGGGGGATCCCCCCCCCCCCTTTGTGGCGGGATTGTGGTC‑TTTCCCGGGCCCCGGTGCCGCCCAACCCCCGCGCCACGCCCCCCCCAAAGGcccaagggg                                         >  2:6636783/1‑143 (MQ=255)

                                                                                                                                 tttGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCg                                                                                         <  2:6842107/83‑1 (MQ=255)

                                                                                                                                 tttGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCg                                                                                         >  1:6842107/1‑83 (MQ=255)

                                                                                                                                   tgtgGGCGGGGCCTTGGGGCGCGCTGGGGGTGCGACGCGCCCCCCACGCTTTGTGCCTGTTTGATTATC‑TGGCGGGGGCCGCTGTGCCGCCCAAGCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTgtgc                    <  2:8209894/150‑1 (MQ=255)

                                                                                                                                      gggAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCt                                                                                       >  1:6478969/1‑80 (MQ=255)

                                                                                                                                                TTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTggc       <  2:4683800‑M1/150‑1 (MQ=255)

                                                                                                                                                    GTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGcc   <  1:377231‑M1/150‑1 (MQ=255)

                                                                                                                                                    GTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGcc   <  1:8450004‑M1/150‑1 (MQ=255)

                                                                                                                                                     TCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAATGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCa  <  2:3569599‑M1/150‑1 (MQ=255)

AAACAGCGCGTTATGTGATTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATTTGTGCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCA  >  NC_002947/5970250‑5970548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`