BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,970,415	A→C	8.0%	L45L (CTT→CTG)	PP_5723 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,970,415	0	A	C	8.0%	424.5 / 13.3	148	L45L (CTT→CTG)	PP_5723	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (17/110); new base C (4/7); total (31/117)
Fisher's exact test for biased strand distribution p-value = 6.44e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 148 total aligned reads displayed.

TTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGTTTCAGT  >  NC_002947/5970268‑5970563

   gCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAgcgc                                                                                                                                                    <  2:218329/150‑1 (MQ=255)

    cTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGTGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGcc                                                                                                                                                   <  1:871432/150‑1 (MQ=255)

      cGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGcccc                                                                                                                                                 <  1:8507596/150‑1 (MQ=255)

       gggATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGccccc                                                                                                                                                <  2:3405711/150‑1 (MQ=255)

         gATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGGGGCGCGGGTGGGGGGGGGAGCGccccccc                                                                                                                                              >  1:2241783/1‑149 (MQ=255)

           ttGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAg                                                                                                                                            <  1:6697335/150‑1 (MQ=255)

           ttGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTGGAAAACTGCACGCGATCTTTATGGGAGCGGCCTTGGGGGGCGGGTGGGGGGGGGAGCGCCCCCCCAg                                                                                                                                            >  2:4007800/1‑150 (MQ=255)

               ccAGTCGCCAGCACGGGGAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGAtt‑t                                                                                                                                       <  1:6688036/150‑1 (MQ=255)

               ccAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGAtt‑t                                                                                                                                       <  2:5917650/150‑1 (MQ=255)

               ccAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGAtt‑t                                                                                                                                       <  2:1741253/150‑1 (MQ=255)

               ccAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCGTGCGTGACGATTTGGGTGCGCAGCGCCCCCCCAGaagt                                                                                                                                        >  1:7754742/1‑147 (MQ=255)

                 aGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑tgt                                                                                                                                     <  2:7679370/150‑1 (MQ=255)

                   tCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑Gc                                                                                                                                  <  1:5299278/150‑1 (MQ=255)

                   tCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑Gc                                                                                                                                  <  2:3835398/150‑1 (MQ=255)

                     gCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCt                                                                                                                                <  1:1645256/150‑1 (MQ=255)

                     gCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGACAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCt                                                                                                                                <  2:7777186/150‑1 (MQ=255)

                      ccAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAACTGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCtt                                                                                                                               <  2:7674150/150‑1 (MQ=255)

                      ccAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCtt                                                                                                                               <  1:8199968/150‑1 (MQ=255)

                      ccAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCtt                                                                                                                               <  2:2886250/150‑1 (MQ=255)

                      ccAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCtt                                                                                                                               <  1:6262341/150‑1 (MQ=255)

                         gCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGGGGCGCGGGAGGGGGGCGGAGCGCCCCCCCAGAGT‑ttt‑ggcgtttt                                                                                                                            >  2:5751070/1‑143 (MQ=255)

                          cACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGAGCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATg                                                                                                                           <  2:9043579/150‑1 (MQ=255)

                           aCGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGc                                                                                                                          <  1:820430/150‑1 (MQ=255)

                             gggAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTg                                                                                                                        <  2:440458/150‑1 (MQ=255)

                             gggAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTg                                                                                                                        <  2:4878119/150‑1 (MQ=255)

                             gggAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTg                                                                                                                        <  2:5208693/150‑1 (MQ=255)

                              ggAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGa                                                                                                                       <  2:1599910/150‑1 (MQ=255)

                              ggAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGa                                                                                                                       <  2:3952319/150‑1 (MQ=255)

                               gAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGGGGCGGGGTTTTGGTGGGGAGCGCCCCCCACCAAT‑TTT‑GGCATGATGCTggt                                                                                                                      >  2:3462704/1‑148 (MQ=255)

                               gAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGGCGCGTTTGGGGGGCGAAGCGCCCCCCCACCTG‑TGT‑GCCGTGATGCTGat                                                                                                                      >  2:7477772/1‑150 (MQ=255)

                                aaaCATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGata                                                                                                                     <  1:8835841/150‑1 (MQ=255)

                                 aaCATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGata‑t                                                                                                                   <  1:4113554/150‑1 (MQ=255)

                                    aTGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGc                                                                                                                <  1:8623826/150‑1 (MQ=255)

                                     tGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGcc                                                                                                               <  1:1178769/150‑1 (MQ=255)

                                      gCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCg                                                                                                              <  1:943700/150‑1 (MQ=255)

                                       cacaATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCgg                                                                                                             <  1:8729829/150‑1 (MQ=255)

                                        acaATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCggg                                                                                                            <  1:5641411/150‑1 (MQ=255)

                                        acaATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCggg                                                                                                            <  1:1491175/150‑1 (MQ=255)

                                         caATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCgggg                                                                                                           <  2:6003429/150‑1 (MQ=255)

                                         caATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCgggg                                                                                                           <  2:9115211/150‑1 (MQ=255)

                                          aaTTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGc                                                                                                          <  2:5439568/150‑1 (MQ=255)

                                          aaTTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGc                                                                                                          <  2:1900569/150‑1 (MQ=255)

                                            tttCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCg                                                                                                        <  1:1044604/150‑1 (MQ=255)

                                              tCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCt                                                                                                      <  2:420454/150‑1 (MQ=255)

                                              tCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCt                                                                                                      <  2:420166/150‑1 (MQ=255)

                                              tCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCt                                                                                                      <  1:9019623/150‑1 (MQ=255)

                                              tCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCt                                                                                                      <  1:9019506/150‑1 (MQ=255)

                                               cGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCtg                                                                                                     <  2:1292203/150‑1 (MQ=255)

                                               cGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCtg                                                                                                     <  2:7527879/150‑1 (MQ=255)

                                               cGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCtg                                                                                                     <  2:7527880/150‑1 (MQ=255)

                                                ggTGCGCATCAGCGTGGCAAGCTGCGCGAAAAATGTTCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCtgt                                                                                                    <  1:5807611/150‑1 (MQ=255)

                                                  tGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGGCGCGCGTGGGGGGCGCCGCGCCCCCCCACAGT‑TGT‑GCCGTGTTGGTGAGAGTTGCCGGGGcgcggggg                                                                                                   >  2:5900466/1‑143 (MQ=255)

                                                   gcgcAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCa                                                                                                 <  1:8110614/150‑1 (MQ=255)

                                                      cagcagCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGcc                                                                                              <  1:5470298/150‑1 (MQ=255)

                                                         gagaaatggccgccagtgtagcacgcacaaacctggcgcctgagtgcacattggcctttgtgtGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCaa                                                                                           >  2:1067401‑M1/64‑150 (MQ=255)

                                                         gagaaatggccgccagtgtagcacgcacaaacctggcgcctgagtgcacattggcctttgtgtGCGGCCTTGCGTCGCGATTGCGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCaa                                                                                           >  2:1067361‑M1/64‑150 (MQ=255)

                                                         cagcGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCaa                                                                                           <  1:7611895/150‑1 (MQ=255)

                                                          agcGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAAt                                                                                          <  2:5802605/150‑1 (MQ=255)

                                                          agcGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAAt                                                                                          <  2:4901596/150‑1 (MQ=255)

                                                          agaaatggccgccagtgtagcacgcacaaacctggcgcctgagtgcacattggcctttgtgtGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAAt                                                                                          >  2:6490043‑M1/63‑150 (MQ=255)

                                                             ggggCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATcgc                                                                                       <  2:2595527/150‑1 (MQ=255)

                                                             aatggccgccagtgtagcacgcacaaacctggcgcctgagtgcacattggcctttgtgtGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATcgc                                                                                       >  1:8020295‑M1/60‑150 (MQ=255)

                                                               tggccgccagtgtagcacgcacaaacctggcgcctgagtgcacattggcctttgtgtGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGa                                                                                     >  1:3232133‑M1/58‑150 (MQ=255)

                                                               ggCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGa                                                                                     <  1:7851626/150‑1 (MQ=255)

                                                               ggCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGa                                                                                     <  2:7185092/150‑1 (MQ=255)

                                                               ggCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGa                                                                                     <  2:619418/150‑1 (MQ=255)

                                                                  aaGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGc                                                                                  <  2:1747968/150‑1 (MQ=255)

                                                                  aaGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGc                                                                                  <  2:6066240/150‑1 (MQ=255)

                                                                    gCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCaa                                                                                <  2:8159421/150‑1 (MQ=255)

                                                                    gCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCaa                                                                                <  1:2940961/150‑1 (MQ=255)

                                                                      tGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAgg                                                                              <  2:1495429/150‑1 (MQ=255)

                                                                         gcgAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGGCGGGATTTGGGGGCGTACCGCCCCCCAACAAT‑TGT‑TGCTTGGTGCGGATA‑ATGGCGGGGCCGCAGTGCCCCCCCAGCCCCACGCAAGGccc                                                                           >  2:8314772/1‑149 (MQ=255)

                                                                         gcgAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGGCGCGAGTGGGGTGCGCAGCCCCCCCCCACAAT‑TTT‑TCCGTGATGCTGGTA‑TTGCCCGGGCCCCGGGGCAGCCCAAAAGCGCCGCAACGCCg                                                                           >  2:8957847/1‑150 (MQ=255)

                                                                         gcgAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCCTCGCGAATTGGGTGCGAAGCCCCCCCCAAGAAT‑TGT‑GCCGTGATTCGGAAA‑TTGCCGGGGCCGCAGTGCAGCCCAATAGCGACGCAACGccc                                                                           >  2:8958016/1‑149 (MQ=255)

                                                                           gAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCGTTCGTGGCGGGTTGGGTGCGGAGCGCCCCCCCAGATT‑TGT‑TGCGTGTTGGTGATA‑TTTGCGGGGGCGCGGTGGGGCCCCAAAGAGACGCCACGCCGca                                                                         >  2:1422905/1‑150 (MQ=255)

                                                                                      aaCAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGGGGGGCGGTTTGGGGGGGGAGCGCCCCCCAAGATT‑TTT‑GGCGTTATGGTGTTA‑ATGCCGGGGGCGCGGGGCAGCCCACTAGCGACGCAACGCCGCACACACAGGccc                                                              >  2:5199059/1‑149 (MQ=255)

                                                                                             tcaaaaCGGCCCGCGATCTTGGGGGGTGCGGCCTGGCGGCGCGTGGGGGGGGCGACCCCCCCCCCCAG‑TT‑TGTGGCCTTCTTGCTCATG‑TGGCCGGGGCGGCTGGGCCGCCCACCCGCCCCGCAAGGCCGCACACAAAGGCCAATGTGCa                                                       <  2:5903101/148‑1 (MQ=255)

                                                                                                               tttGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCg                                                                                                        >  1:6842107/1‑83 (MQ=255)

                                                                                                               tttGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCg                                                                                                        <  2:6842107/83‑1 (MQ=255)

                                                                                                                    gggAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCt                                                                                                      >  1:6478969/1‑80 (MQ=255)

                                                                                                                    gggAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCt                                                                                                      <  2:6478969/80‑1 (MQ=255)

                                                                                                                     gctgCGGCCTTGCGTCGGTTGTGGGGGGCTACGC‑CCCCCCAATTTTGTGT‑GCGTTTTGGCTGATT‑TGGGGGGGGCCGCGGTGCACCCCAAGCGCGCCCCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTgtgcgtgc                               <  2:5488253/147‑1 (MQ=255)

                                                                                                                              TTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTggc                      <  2:4683800‑M1/150‑1 (MQ=255)

                                                                                                                                  GTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGcc                  <  1:2310209‑M1/150‑1 (MQ=255)

                                                                                                                                  GTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGcc                  <  1:377229‑M1/150‑1 (MQ=255)

                                                                                                                                  GTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGcc                  <  1:377231‑M1/150‑1 (MQ=255)

                                                                                                                                   TCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAATGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCa                 <  2:3569599‑M1/150‑1 (MQ=255)

                                                                                                                                   TCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCa                 <  2:3569544‑M1/150‑1 (MQ=255)

                                                                                                                                   TCGCGATTGGGGTGCGAAGCGCCCCCAAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCa                 <  2:6794758‑M1/150‑1 (MQ=255)

                                                                                                                                    aaatcTTGGGGGGCGCTTCGCACCCCAATATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCAt                <  2:4092114‑M1/145‑1 (MQ=255)

                                                                                                                                    aaatcTTGGGGGGCGCTTCGCACCCCAATATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCAt                <  2:2390748‑M1/145‑1 (MQ=255)

                                                                                                                                      atcTTGGGGGGCGCTTCGCACCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCAttt              <  1:8976381‑M1/147‑1 (MQ=255)

                                                                                                                                      atcTTGGGGGGCGCTTCGCACCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCAttt              <  1:9157706‑M1/147‑1 (MQ=255)

                                                                                                                                        cTTGGGGGGCGCTTCGCACCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTtct            <  1:5047171‑M1/149‑1 (MQ=255)

                                                                                                                                        ATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTtct            <  2:5896732‑M1/150‑1 (MQ=255)

                                                                                                                                            GGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTa                             <  1:4955999‑M1/129‑1 (MQ=255)

                                                                                                                                            GGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGt        <  1:1123844‑M1/150‑1 (MQ=255)

                                                                                                                                             GGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGgc                                                >  1:5535799‑M1/1‑109 (MQ=255)

                                                                                                                                             GGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGACCATTTCTCCgtg       <  1:6388326‑M1/150‑2 (MQ=255)

                                                                                                                                                gCGACGCCCCCCCCACTTTT‑GGT‑GCCTTGAGGCTTTTT‑GTGCCGGGGCCGCTGCGCAGCCAAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGTTTCa    <  2:8253174/150‑1 (MQ=255)

TTTGCTCGGGATTGGCCAGTCGCCAGCACGGGAAACATGCACAATTTCGGTGCGCAGCAGCGGGGCAAGCTGCGCGAAAAATGTGCAACAGCTTGAAAACTGCACGCGATCTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGGTGCGAAGCGCCCCCCAAGATT‑TGT‑GCCTTGATGCTGATA‑TTGCCGGGGCCGCTGTGCAGCCCAATCGCGACGCAAGGCCGCACACAAAGGCCAATGTGCACTCAGGCGCCAGGTTTGTGCGTGCTACACTGGCGGCCATTTCTCCGTTTCAGT  >  NC_002947/5970268‑5970563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`