BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,971,711	T→A	8.0%	N521Y (AAC→TAC)	PP_5236 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,971,711	0	T	A	8.0%	962.8 / 54.0	301	N521Y (AAC→TAC)	PP_5236	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (125/151); new base A (6/18); total (132/169)
Fisher's exact test for biased strand distribution p-value = 8.42e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.35e-01

Only 100 of 304 total aligned reads displayed.

CCGGCGTAACAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGTTCAGTTCGG  >  NC_002947/5971565‑5971860

 cGGCGTAACAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTAc                                                                                                                                                   >  1:2753712/1‑150 (MQ=255)

  ggCGTAACAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACt                                                                                                                                                  <  2:4492100/150‑1 (MQ=255)

     gTAACAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTgg                                                                                                                                               <  1:5214354/150‑1 (MQ=255)

       aaCAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcc                                                                                                                                             >  1:3400242/1‑150 (MQ=255)

        aCAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcct                                                                                                                                            >  2:3106561/1‑150 (MQ=255)

        aCAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAAGTACAACCTGgcct                                                                                                                                            <  1:8491576/150‑1 (MQ=255)

        aCAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAAGTACAACCTGgcct                                                                                                                                            <  2:5109096/150‑1 (MQ=255)

        aCAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCACAGGCAGGCCAGGTTGTACTTGgcct                                                                                                                                            >  1:4502782/1‑150 (MQ=255)

        aCAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGAGCAGGCAGGCCAAGTACAACCTGgcct                                                                                                                                            <  2:3576741/150‑1 (MQ=255)

          aaaaTCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcctgc                                                                                                                                          <  2:8389981/150‑1 (MQ=255)

          aaaaTCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcctgc                                                                                                                                          <  1:6446136/150‑1 (MQ=255)

           aaaTCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcctgcc                                                                                                                                         <  1:102451/150‑1 (MQ=255)

           aaaTCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGAGCAGGCAGGCCAAGTACAACCTGgcctgcc                                                                                                                                         <  1:1726672/150‑1 (MQ=255)

            aaTCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcctgcct                                                                                                                                        <  2:8313995/150‑1 (MQ=255)

            aaTCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcctgcct                                                                                                                                        <  1:4194780/150‑1 (MQ=255)

                 ccAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCGGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAAGTACAACCTGGCctgcctgcgca                                                                                                                                   <  1:5048872/150‑4 (MQ=39)

                 ccAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCtgctgc                                                                                                                                   <  2:2229009/150‑1 (MQ=255)

                 ccAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCtgctgc                                                                                                                                   >  2:1465755/1‑150 (MQ=255)

                 ccAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTGGTACTGGGCCTGCCtgctgc                                                                                                                                   >  2:6192346/1‑150 (MQ=255)

                   aGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGTTTGTACTTGGCCTGCCTGCTGccc                                                                                                                                 <  1:3264717/150‑1 (MQ=255)

                   aGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGccc                                                                                                                                 >  1:7174559/1‑150 (MQ=255)

                          aCCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCTGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCa                                                                                                                          <  1:1209496/150‑1 (MQ=255)

                          aCCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCa                                                                                                                          <  2:6541287/150‑1 (MQ=255)

                           ccGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGTGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCAc                                                                                                                         <  2:783470/150‑1 (MQ=255)

                            cgcgCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCAcc                                                                                                                        <  2:1768194/150‑1 (MQ=255)

                                 acacGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACg                                                                                                                   >  2:208622/1‑150 (MQ=255)

                                  cacGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGc                                                                                                                  <  2:2196605/150‑1 (MQ=255)

                                     gCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGc                                                                                                               >  2:2376941/1‑150 (MQ=255)

                                     gCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGc                                                                                                               >  1:60126/1‑150 (MQ=255)

                                      cATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCa                                                                                                              <  2:7931171/150‑1 (MQ=255)

                                       aTTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGCCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTgg                                                                                                                                               <  1:4024860/116‑1 (MQ=255)

                                        ttGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGc                                                                                                            >  2:1186894/1‑150 (MQ=255)

                                          ggTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCa                                                                                                          <  2:1636114/150‑1 (MQ=255)

                                                cccGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCGGCCAGTAccc                                                                                                    <  1:7401957/150‑1 (MQ=255)

                                                cccGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCGGCCAGTAccc                                                                                                    >  2:7401957/1‑150 (MQ=255)

                                                cccGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTAccc                                                                                                    >  1:2702574/1‑150 (MQ=255)

                                                 ccGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCg                                                                                                   <  2:2883143/150‑1 (MQ=255)

                                                  cGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCgg                                                                                                  >  1:1968730/1‑150 (MQ=255)

                                                   gTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGc                                                                                                 >  1:2821181/1‑150 (MQ=255)

                                                       cccAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTAc                                                                                                                                                   >  2:4815302/1‑96 (MQ=255)

                                                        ccAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCt                                                                                            <  1:8190604/150‑1 (MQ=255)

                                                        ccAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCt                                                                                            <  1:3577524/150‑1 (MQ=255)

                                                        ccAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCAGGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCt                                                                                            >  2:7306817/1‑150 (MQ=255)

                                                         cAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCCGTACCCGGCTGCCTc                                                                                           <  2:2732811/150‑1 (MQ=255)

                                                         cAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTc                                                                                           <  2:8649598/150‑1 (MQ=255)

                                                                tCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGtcga                                                                                                                                                  >  1:6310399/1‑85 (MQ=255)

                                                                tCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGtcga                                                                                                                                                  <  2:6311289/88‑4 (MQ=255)

                                                                    cAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGt                                                                                >  1:3934150/1‑150 (MQ=255)

                                                                     aaGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTa                                                                               >  1:3671645/1‑150 (MQ=255)

                                                                     aaGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTa                                                                               <  1:2550239/150‑1 (MQ=255)

                                                                     aaGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTa                                                                               >  2:8482941/1‑150 (MQ=255)

                                                                     aaGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCAGCCAGGTa                                                                               >  1:5974234/1‑150 (MQ=255)

                                                                      aGCAGGCGCCTGACCGCCAGCTTGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCTTGCGCAGGCTGGCCTGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTaa                                                                              <  1:2268847/150‑1 (MQ=255)

                                                                       gCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAAt                                                                             >  2:8916747/1‑150 (MQ=255)

                                                                        cAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATg                                                                            >  2:5365185/1‑150 (MQ=255)

                                                                          ggCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATgcg                                                                          >  1:9002703/1‑150 (MQ=255)

                                                                             gcCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCg                                                                       >  1:2934871/1‑150 (MQ=255)

                                                                               cTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCggg                                                                     >  2:1999947/1‑150 (MQ=255)

                                                                               cTGACCGCCAGCAGGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCggg                                                                     <  1:321484/150‑1 (MQ=255)

                                                                                tGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCgggg                                                                    >  1:8699409/1‑150 (MQ=255)

                                                                                tGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCgggg                                                                    <  1:1486908/150‑1 (MQ=255)

                                                                                    cGCCATCATGGCACTGCGCTCGTTCATATCATCATCGCCGTCCATGCGCAGGCAGGCCAGGTTTTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGtt                                                                <  1:65936/150‑1 (MQ=255)

                                                                                    cGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGtt                                                                >  1:4815206/1‑150 (MQ=255)

                                                                                      ccAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTgg                                                              >  1:3423880/1‑150 (MQ=255)

                                                                                       cAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGc                                                             <  2:6494735/150‑1 (MQ=255)

                                                                                         gCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCa                                                           <  1:5423368/150‑1 (MQ=255)

                                                                                          cATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAg                                                          <  1:1341484/150‑1 (MQ=255)

                                                                                           aTGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAgg                                                         <  1:7973252/150‑1 (MQ=255)

                                                                                             ggCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGgcc                                                       <  2:6045578/150‑1 (MQ=255)

                                                                                                aCTGCGCTCGTTCAGATCATCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGgccgcc                                                    >  2:4009814/1‑150 (MQ=255)

                                                                                                  tGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGCGTTGGCCAGGCCGCCAt                                                  <  1:7852770/150‑1 (MQ=255)

                                                                                                          ttCAGATCCTCATGGCCGTCCATGCGCTGGCAGGCCAGGTTGTTCTTGGCCTGCCTGCTGCCCGCCTCCACCGCACTCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGc                                          <  1:2342409/150‑1 (MQ=255)

                                                                                                          ttCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGc                                          >  1:1756917/1‑150 (MQ=255)

                                                                                                          ttCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGc                                          >  1:4641601/1‑150 (MQ=255)

                                                                                                          ttCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGc                                          >  1:5013230/1‑150 (MQ=255)

                                                                                                          ttCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGc                                          >  1:5777697/1‑150 (MQ=255)

                                                                                                             aGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCa                                       <  1:1172351/150‑1 (MQ=255)

                                                                                                             aGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCa                                       <  1:4329904/150‑1 (MQ=255)

                                                                                                                tCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATAc                                    >  2:8437258/1‑150 (MQ=255)

                                                                                                                 ccTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTTCCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACa                                   >  1:6991436/1‑150 (MQ=255)

                                                                                                                 ccTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACa                                   >  1:677639/1‑150 (MQ=255)

                                                                                                                  cTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACaa                                  >  1:2126228/1‑150 (MQ=255)

                                                                                                                   tCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAg                                 >  2:5598338/1‑150 (MQ=255)

                                                                                                                   tCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAg                                 >  2:4422883/1‑150 (MQ=255)

                                                                                                                         ccGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAgg                           >  1:6354793/1‑150 (MQ=255)

                                                                                                                              cATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGgcagc                      <  1:8433902/150‑1 (MQ=255)

                                                                                                                                    caggcaggCCAAGTACAACCTGGCCTGCCCGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATgg                                                <  2:665983/118‑1 (MQ=38)

                                                                                                                                    caggcaggCCAAGTACAACCTGGCCTGCCCGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATgg                                                >  1:665983/1‑118 (MQ=38)

                                                                                                                                     aggcaggcCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGGACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGc               <  2:7174559/150‑1 (MQ=255)

                                                                                                                                       gcaggcCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAg             >  1:8896693/1‑150 (MQ=255)

                                                                                                                                       gcaggcCAAGTACAACCTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAg             >  1:4925438/1‑150 (MQ=255)

                                                                                                                                       acaggcCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAg             <  1:2548621/149‑1 (MQ=255)

                                                                                                                                        caggcCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCCCCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAgg            <  2:4887623/150‑1 (MQ=255)

                                                                                                                                        caggcCAAGTACAACCTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAgg            <  1:1666103/150‑1 (MQ=255)

                                                                                                                                         aggcCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGt           <  2:2472341/150‑1 (MQ=255)

                                                                                                                                          ggcCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGtt          >  2:6994887/1‑150 (MQ=255)

                                                                                                                                          ggcCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGtt          <  1:6909738/150‑1 (MQ=255)

                                                                                                                                           gcCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGTTc         <  1:608411/150‑1 (MQ=255)

                                                                                                                                                gTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGTTCAGTTc    >  2:3799334/1‑150 (MQ=255)

                                                                                                                                                  tGTACTTGGCCGGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGTTCAGTTCgg  <  2:3648545/150‑1 (MQ=255)

CCGGCGTAACAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGTTCAGTTCGG  >  NC_002947/5971565‑5971860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`