BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,986,893	G→A	5.4%	L69L (CTG→TTG)	ycaC‑II ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,986,893	0	G	A	5.4%	1116.2 / 29.6	333	L69L (CTG→TTG)	ycaC‑II	putative hydrolase
Reads supporting (aligned to +/- strand): ref base G (133/182); new base A (9/9); total (142/191)
Fisher's exact test for biased strand distribution p-value = 6.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 334 total aligned reads displayed.

ACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGA  >  NC_002947/5986745‑5987042

         accacACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTc                                                                                                                                             >  1:8762505/1‑150 (MQ=255)

           cacaCCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCgg                                                                                                                                           <  2:5955981/150‑1 (MQ=255)

           cacaCCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCgg                                                                                                                                           >  1:1597012/1‑150 (MQ=255)

           cacaCCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCgg                                                                                                                                           >  1:5614922/1‑150 (MQ=255)

             cacCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGc                                                                                                                                         <  1:8331738/150‑1 (MQ=255)

             cacCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGc                                                                                                                                         <  2:7774368/150‑1 (MQ=255)

                    gatgatCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTc                                                                                                                                  >  1:1933730/1‑150 (MQ=255)

                    gatgatCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTc                                                                                                                                  >  2:4436954/1‑150 (MQ=255)

                     atgatCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCg                                                                                                                                 >  2:5505247/1‑150 (MQ=255)

                       gatCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGaa                                                                                                                               <  2:2378896/150‑1 (MQ=255)

                       gatCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGaa                                                                                                                               <  1:4621951/150‑1 (MQ=255)

                            gTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtgg                                                                                                                          >  2:2130653/1‑150 (MQ=255)

                              tgcttgcGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtggtg                                                                                                                        <  1:900472/150‑1 (MQ=255)

                                 ttgcGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGgtggtg                                                                                                                     >  1:4935903/1‑150 (MQ=255)

                                 ttgcGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGgtggtg                                                                                                                     >  1:8631678/1‑150 (MQ=255)

                                  tcttgCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTggtggtca                                                                                                                    <  1:7909991/146‑3 (MQ=2)

                                     ggCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGa                                                                                                                 >  2:4314046/1‑150 (MQ=255)

                                     ggCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGa                                                                                                                 <  1:1012923/150‑1 (MQ=255)

                                     ggCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGa                                                                                                                 <  1:3002197/150‑1 (MQ=255)

                                      gCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGAt                                                                                                                <  1:6959611/150‑1 (MQ=255)

                                       ccggTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTGGTGGTCAgaaca                                                                                                               <  2:3273256/147‑5 (MQ=2)

                                       ccggTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTGGTGGTCAgaaca                                                                                                               <  2:7890316/147‑5 (MQ=2)

                                       cccGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATg                                                                                                               <  2:6964652/150‑1 (MQ=255)

                                       cccGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATg                                                                                                               <  1:5427325/150‑1 (MQ=255)

                                         ggTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTGGTGGTCAgaacagt                                                                                                             <  1:1514744/149‑7 (MQ=2)

                                         cGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGt                                                                                                             >  1:1718980/1‑150 (MQ=255)

                                          gTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTc                                                                                                            <  2:4940493/150‑1 (MQ=255)

                                            ggCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCgg                                                                                                          <  1:965575/150‑1 (MQ=255)

                                             gCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGc                                                                                                         >  2:6464226/1‑150 (MQ=255)

                                              ccTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCa                                                                                                        <  2:6610663/150‑1 (MQ=255)

                                                ttcactGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTGGTGGTCAGAACAGTCGGCAgc                                                                                                      >  1:7426027/6‑149 (MQ=2)

                                                ttGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAgg                                                                                                      >  2:4760162/1‑150 (MQ=255)

                                                ttGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAgg                                                                                                      <  1:6642685/150‑1 (MQ=255)

                                                   actGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTGGTGGTCAGAACAGTCGGCAgcttg                                                                                                   >  2:2738654/3‑146 (MQ=2)

                                                     tGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTGGTGGTCAGAACAGTCGGCAgcttgaa                                                                                                 <  1:5131812/150‑7 (MQ=2)

                                                     tGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCaaa                                                                                                 <  2:36739/150‑1 (MQ=255)

                                                     tGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCaaa                                                                                                 <  2:3541212/150‑1 (MQ=255)

                                                        cTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGaa                                                                                              <  2:1315379/150‑1 (MQ=255)

                                                         ttGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAAc                                                                                             >  2:8762389/1‑150 (MQ=255)

                                                            aCGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg                                                                                          >  2:2454190/1‑150 (MQ=255)

                                                              gAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGc                                                                                        >  2:1342329/1‑150 (MQ=255)

                                                                aGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGCGAGGATGGTCGGCAGGCCAAAGAACTTGGCCa                                                                                      <  2:7235462/150‑1 (MQ=255)

                                                                  tCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAgg                                                                                    <  2:3170462/150‑1 (MQ=255)

                                                                    ttCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTc                                                                                  >  1:7053919/1‑150 (MQ=255)

                                                                         tGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCa                                                                             >  2:5100612/1‑150 (MQ=255)

                                                                           tCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAg                                                                           <  2:814294/150‑1 (MQ=255)

                                                                             ccAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCCCCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGc                                                                         >  1:1978544/1‑150 (MQ=255)

                                                                             ccAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGc                                                                         >  1:6992047/1‑150 (MQ=255)

                                                                             ccAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGc                                                                         <  2:7614645/150‑1 (MQ=255)

                                                                              cAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGcc                                                                        >  1:4752853/1‑150 (MQ=255)

                                                                                   gTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGGCAGGTCGCCCAAGGCCAGCAc                                                                   >  2:5689867/1‑150 (MQ=255)

                                                                                   gTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCAc                                                                   >  2:7585457/1‑150 (MQ=255)

                                                                                      gATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACgtt                                                                <  1:1413195/150‑1 (MQ=255)

                                                                                        tCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAg                                                                                                                              >  1:1307859/1‑86 (MQ=255)

                                                                                          tGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTc                                                            <  1:6122496/150‑1 (MQ=255)

                                                                                           ggCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCt                                                           <  2:3072791/150‑1 (MQ=255)

                                                                                            gCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCtt                                                          <  2:7038244/150‑1 (MQ=255)

                                                                                            gCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCtt                                                          >  2:6002381/1‑150 (MQ=255)

                                                                                            gCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCtt                                                          >  2:4621252/1‑150 (MQ=255)

                                                                                              cGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGa                                                        >  1:9043426/1‑150 (MQ=255)

                                                                                                ggACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAAt                                                      >  2:4290898/1‑150 (MQ=255)

                                                                                                  aCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTc                                                    <  1:3785054/150‑1 (MQ=255)

                                                                                                    gggCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGt                                                  <  2:5810165/150‑1 (MQ=255)

                                                                                                    gggCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGt                                                  >  1:4528607/1‑150 (MQ=255)

                                                                                                     ggCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGtt                                                 <  2:9094290/150‑1 (MQ=255)

                                                                                                     ggCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGtt                                                 >  1:888499/1‑150 (MQ=255)

                                                                                                     ggCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGtt                                                 <  1:6348515/150‑1 (MQ=255)

                                                                                                      gCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTg                                                <  2:1687798/150‑1 (MQ=255)

                                                                                                      gCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTg                                                <  1:330098/150‑1 (MQ=255)

                                                                                                         aTGTACGGCGCATCCGGGAAGATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGc                                             <  2:1990089/150‑1 (MQ=255)

                                                                                                         aTGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAATAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGc                                             <  1:7186564/150‑1 (MQ=255)

                                                                                                         aTGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGc                                             <  2:391324/150‑1 (MQ=255)

                                                                                                         aTGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGc                                             <  1:3180005/150‑1 (MQ=255)

                                                                                                                gcgcATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAAt                                      >  1:4249354/1‑150 (MQ=255)

                                                                                                                gcgcATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAAt                                      >  1:4352222/1‑150 (MQ=255)

                                                                                                                 cgcATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATc                                     <  1:5878592/150‑1 (MQ=255)

                                                                                                                   cATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCt                                   <  2:6348061/150‑1 (MQ=255)

                                                                                                                   cATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCt                                   <  1:2422193/150‑1 (MQ=255)

                                                                                                                    aTCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTg                                  <  1:4436954/150‑1 (MQ=255)

                                                                                                                    aTCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTg                                  <  1:7694894/150‑1 (MQ=255)

                                                                                                                    aTCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTg                                  <  1:5223013/150‑1 (MQ=255)

                                                                                                                     tCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGc                                 >  2:5187922/1‑150 (MQ=255)

                                                                                                                       cGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCAc                               <  1:2820749/150‑1 (MQ=255)

                                                                                                                        gggAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCAcc                              <  2:2861712/150‑1 (MQ=255)

                                                                                                                        gggAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCAcc                              >  2:6708301/1‑150 (MQ=255)

                                                                                                                         ggAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCa                             >  2:5639370/1‑150 (MQ=255)

                                                                                                                             cATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCga                         <  2:4112214/150‑1 (MQ=255)

                                                                                                                              aTCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCgag                        >  1:8529949/1‑150 (MQ=255)

                                                                                                                              aTCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCgag                        >  2:6163045/1‑150 (MQ=255)

                                                                                                                               tctcTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCt                                   >  1:3624856/1‑138 (MQ=255)

                                                                                                                                ctctTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGAt                      <  2:6126246/150‑1 (MQ=255)

                                                                                                                                  ctTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCa                    <  1:6422401/150‑1 (MQ=255)

                                                                                                                                   tttCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAg                   >  1:2129509/1‑150 (MQ=255)

                                                                                                                                   tttCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAg                   >  1:2650938/1‑150 (MQ=255)

                                                                                                                                     tCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGAc                 >  2:6411513/1‑150 (MQ=255)

                                                                                                                                         cTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGt             <  2:1609871/150‑1 (MQ=255)

                                                                                                                                               cACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCtggtg       >  1:3020130/1‑150 (MQ=255)

                                                                                                                                                aCCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCtggtgg      >  2:8480422/1‑150 (MQ=255)

                                                                                                                                                  cAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTc    <  2:1463753/150‑1 (MQ=255)

                                                                                                                                                  cAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTc    <  1:7954938/150‑1 (MQ=255)

ACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGA  >  NC_002947/5986745‑5987042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`