BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,986,918	G→A	5.4%	S60S (AGC→AGT)	ycaC‑II ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,986,918	0	G	A	5.4%	1117.2 / 29.9	332	S60S (AGC→AGT)	ycaC‑II	putative hydrolase
Reads supporting (aligned to +/- strand): ref base G (135/179); new base A (9/9); total (144/188)
Fisher's exact test for biased strand distribution p-value = 6.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 339 total aligned reads displayed.

ATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGACCAGCAGCACCACGGCGTCGTCTTT  >  NC_002947/5986769‑5987067

 tCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGc                                                                                                                                                      >  1:6849007/1‑150 (MQ=255)

  cAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCt                                                                                                                                                     <  1:4566758/150‑1 (MQ=255)

  cAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCt                                                                                                                                                     <  1:196113/150‑1 (MQ=255)

     ttgcttgcGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtggt                                                                                                                                                  <  1:6342745/150‑1 (MQ=255)

     ttgcttgcGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtggt                                                                                                                                                  >  2:783113/1‑150 (MQ=255)

      tgcttgcGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtggtg                                                                                                                                                 <  1:8182542/150‑1 (MQ=255)

       gcttgcGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtggtgg                                                                                                                                                >  2:4817021/1‑150 (MQ=255)

         ttgcGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGgtggtg                                                                                                                                              >  1:8631678/1‑150 (MQ=255)

         ttcttgCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTggtggtc                                                                                                                                              >  1:8895561/6‑149 (MQ=2)

          tgcGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGa                                                                                                                                             <  1:6399159/150‑1 (MQ=255)

          tgcGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGa                                                                                                                                             <  1:3293593/150‑1 (MQ=255)

          tcttgCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTggtggtca                                                                                                                                             <  1:7909991/146‑3 (MQ=2)

           gcGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAg                                                                                                                                            <  1:6007703/150‑1 (MQ=255)

            ttgCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTGGTGGTCAga                                                                                                                                           >  1:2914262/3‑149 (MQ=2)

             ggCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGa                                                                                                                                          <  1:1012923/150‑1 (MQ=255)

               ccggTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTGGTGGTCAgaaca                                                                                                                                        <  2:3273256/147‑5 (MQ=2)

               cccGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATg                                                                                                                                        <  1:5427325/150‑1 (MQ=255)

                ccGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATgg                                                                                                                                       >  1:3040692/1‑150 (MQ=255)

                 ggTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTGGTGGTCAgaacagt                                                                                                                                      <  1:1514744/149‑7 (MQ=2)

                 cGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGt                                                                                                                                      >  1:1718980/1‑150 (MQ=255)

                 cGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGt                                                                                                                                      <  2:9059175/150‑1 (MQ=255)

                  gTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTc                                                                                                                                     <  2:4940493/150‑1 (MQ=255)

                  gTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTGGTGGTCAgaacagtc                                                                                                                                     <  2:838123/150‑8 (MQ=2)

                    ggCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCgg                                                                                                                                   <  1:965575/150‑1 (MQ=255)

                      ccTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCa                                                                                                                                 <  2:1338383/150‑1 (MQ=255)

                      ccTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCa                                                                                                                                 <  2:3185543/150‑1 (MQ=255)

                       cTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAg                                                                                                                                >  1:7948562/1‑150 (MQ=255)

                          gATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGcc                                                                                                                             <  1:300449/150‑1 (MQ=255)

                             tGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACTGGTGGTCAGAACAGTCGGCAgcttgaa                                                                                                                          <  1:5131812/150‑7 (MQ=2)

                             tGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCaaa                                                                                                                          <  2:5505722/150‑1 (MQ=255)

                             tGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCaaa                                                                                                                          <  2:36739/150‑1 (MQ=255)

                               ccTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGa                                                                                                                        <  2:4662381/150‑1 (MQ=255)

                                 ttGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAAc                                                                                                                      <  2:6461741/150‑1 (MQ=255)

                                 ttGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAAc                                                                                                                      >  2:8762389/1‑150 (MQ=255)

                                   gACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACtt                                                                                                                    <  1:7500451/150‑1 (MQ=255)

                                    ccgcaGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg                                                                                                                   <  2:6886473/146‑1 (MQ=255)

                                    aCGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg                                                                                                                   <  2:6144966/150‑1 (MQ=255)

                                    aCGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg                                                                                                                   >  2:2454190/1‑150 (MQ=255)

                                      gAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGc                                                                                                                 >  1:4907229/1‑150 (MQ=255)

                                      gAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGc                                                                                                                 >  2:2820749/1‑150 (MQ=255)

                                         gTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAg                                                                                                              <  2:424936/150‑1 (MQ=255)

                                         gTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAg                                                                                                              <  2:2065287/150‑1 (MQ=255)

                                            ttCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTc                                                                                                           >  2:5982808/1‑150 (MQ=255)

                                                  gTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCaa                                                                                                     <  2:1542811/150‑1 (MQ=255)

                                                   tCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAg                                                                                                    <  2:814294/150‑1 (MQ=255)

                                                     ccAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGc                                                                                                  <  2:7614645/150‑1 (MQ=255)

                                                      cAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGcc                                                                                                 >  1:4752853/1‑150 (MQ=255)

                                                           gTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGGCAGGTCGCCCAAGGCCAGCAc                                                                                            >  2:5689867/1‑150 (MQ=255)

                                                           gTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCAc                                                                                            >  2:7585457/1‑150 (MQ=255)

                                                              gATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACgtt                                                                                         <  2:3093076/150‑1 (MQ=255)

                                                                tCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAg                                                                                                                                                       >  1:1307859/1‑86 (MQ=255)

                                                                tCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAg                                                                                                                                                       <  2:1307859/86‑1 (MQ=255)

                                                                    gCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCtt                                                                                   <  2:7038244/150‑1 (MQ=255)

                                                                    gCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCtt                                                                                   <  2:6696465/150‑1 (MQ=255)

                                                                    gCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCtt                                                                                   <  2:3567784/150‑1 (MQ=255)

                                                                    gCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCtt                                                                                   <  1:5056447/150‑1 (MQ=255)

                                                                      cGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGa                                                                                 >  1:9043426/1‑150 (MQ=255)

                                                                          aCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTc                                                                             <  1:8762389/150‑1 (MQ=255)

                                                                            gggCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGt                                                                           <  2:5810165/150‑1 (MQ=255)

                                                                            gggCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGt                                                                           >  1:4528607/1‑150 (MQ=255)

                                                                             ggCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGtt                                                                          <  1:5252530/150‑1 (MQ=255)

                                                                             ggCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGtt                                                                          <  1:7020597/150‑1 (MQ=255)

                                                                             ggCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGtt                                                                          >  1:6966990/1‑150 (MQ=255)

                                                                             ggCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGtt                                                                          <  1:6539271/150‑1 (MQ=255)

                                                                             ggCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGtt                                                                          <  1:4404796/150‑1 (MQ=255)

                                                                              gCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGTGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTg                                                                         <  2:1687483/150‑1 (MQ=255)

                                                                              gCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTg                                                                         <  1:330098/150‑1 (MQ=255)

                                                                               cAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTgg                                                                        >  2:6079051/1‑150 (MQ=255)

                                                                               cAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTgg                                                                        >  2:5122777/1‑150 (MQ=255)

                                                                                 aTGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAATAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGc                                                                      <  1:7186564/150‑1 (MQ=255)

                                                                                        gcgcATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAAt                                                               >  1:4249354/1‑150 (MQ=255)

                                                                                         cgcATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATc                                                              <  1:5878592/150‑1 (MQ=255)

                                                                                         cgcATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATc                                                              >  2:5356778/1‑150 (MQ=255)

                                                                                           cATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCt                                                            <  1:1395731/150‑1 (MQ=255)

                                                                                            aTCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTg                                                           <  1:7694894/150‑1 (MQ=255)

                                                                                                     cATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCga                                                  <  2:4112214/150‑1 (MQ=255)

                                                                                                      aTCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCgag                                                 >  1:8529949/1‑150 (MQ=255)

                                                                                                       tctcTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCgaga                                                <  2:6386663/150‑1 (MQ=255)

                                                                                                       tctcTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCgaga                                                <  2:6580975/150‑1 (MQ=255)

                                                                                                         tctTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATc                                              >  1:2368871/1‑150 (MQ=255)

                                                                                                         tctTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATc                                              >  2:2737097/1‑150 (MQ=255)

                                                                                                           tttCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAg                                            >  1:2650968/1‑150 (MQ=255)

                                                                                                           tttCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAg                                            >  1:2128877/1‑150 (MQ=255)

                                                                                                               aGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCg                                        >  1:3780015/1‑150 (MQ=255)

                                                                                                                     ggCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCtgg                                  <  2:4472564/150‑1 (MQ=255)

                                                                                                                           aGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCga                                                  >  1:2436160/1‑128 (MQ=255)

                                                                                                                            gCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGa                           <  2:6226597/150‑1 (MQ=255)

                                                                                                                               ggCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGACca                        <  2:5092242/150‑1 (MQ=255)

                                                                                                                                         gCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGACCAGCAGcaccac              <  2:5598644/150‑1 (MQ=255)

                                                                                                                                           cTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGACCAGCAGCACCACgg            <  2:1386309/150‑1 (MQ=255)

                                                                                                                                           cTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGACCAGCAGCACCACgg            >  1:9004918/1‑150 (MQ=255)

                                                                                                                                           cTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGACCAGCAGCACCACgg            >  1:1594712/1‑150 (MQ=255)

                                                                                                                                           cTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGACCAGCAGCACCACgg            >  2:7177695/1‑150 (MQ=255)

                                                                                                                                           cTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGACCAGCAGCACCACgg            <  2:3958170/150‑1 (MQ=255)

                                                                                                                                                tCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGACCAGCAGCACCACGGcgtcg       >  1:3538977/1‑150 (MQ=255)

                                                                                                                                                tCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGACCAGCAGCACCACGGcgtcg       >  2:1906806/1‑150 (MQ=255)

                                                                                                                                                tCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGACCAGCAGCACCACGGcgtcg       >  1:7095274/1‑150 (MQ=255)

                                                                                                                                                 cGAAGCTGGTGGTGAGGATGGTCGGCATGCCAAAGAACTTGGCCAGGTCGCCCAAGGACAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCCGCGAGATCAGACCGGTCTGGTGGTCGACCAGCAGCACCACGGcgtcgt      <  2:43300/150‑1 (MQ=255)

                                                                                                                                                  gAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGACCAGCAGCACCACGGCgtcgtc     >  2:2195844/1‑150 (MQ=255)

                                                                                                                                                     gCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGACCAGCAGCACCACGGCGTCGTCttt  <  1:430949/150‑1 (MQ=255)

ATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATTCGTTGGGCGAAAAATCCTGCACCAGCGAGATCAGACCGGTCTGGTGGTCGACCAGCAGCACCACGGCGTCGTCTTT  >  NC_002947/5986769‑5987067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`