Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 471,555 C→T 7.1% E461E (GAG→GAA)  rpoD ← RNA polymerase sigma 70 factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947471,5550CT 7.1% 952.1 / 41.3 310E461E (GAG→GAA) rpoDRNA polymerase sigma 70 factor
Reads supporting (aligned to +/- strand):  ref base C (138/150);  new base T (8/14);  total (146/164)
Fisher's exact test for biased strand distribution p-value = 3.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 318 total aligned reads displayed.

CATGGAGATCGGCTCTTTGGCGATCTTCAATACCTTGCGGATTTTGTCCTCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTT  >  NC_002947/471408‑471704
                                                                                                                                                   |                                                                                                                                                     
cATGGAGATCGGCTCTTTGGCGATCTTCAATACCTTGCGGATTTTGTCCTCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGtctc                                                                                                                                                     <  2:6718588/150‑1 (MQ=255)
 aTGGAGATCGGCTCTTTGGCGATCTTCAATACCTTGCGGATTTTGTCCTCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGAAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCg                                                                                                                                                    >  1:7035662/1‑150 (MQ=255)
  tGGAGATCGGCTCTTTGGCGATCTTCAATACCTTGCGGATTTTGTCCTCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGa                                                                                                                                                   >  2:7151791/1‑150 (MQ=255)
  tGGAGATCGGCTCTTTGGCGATCTTCAATACCTTGCGGATTTTGTCCTCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGa                                                                                                                                                   <  2:1061382/150‑1 (MQ=255)
   ggAGATCGGCTCTTTGGCGATCTTCAATACCTTGCGGATTTTGTCCTCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGAt                                                                                                                                                  >  2:3234057/1‑150 (MQ=255)
     agaTCGGCTCTTTGGCGATCTTCAATACCTTGCGGATTTTGTCCTCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCa                                                                                                                                                <  2:1242179/150‑1 (MQ=255)
      gaTCGGCTCTTTGGCGATCTTCAATACCTTGCGGATTTTGTCCTCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCAt                                                                                                                                               <  2:6255926/150‑1 (MQ=255)
        tCGGCTCTTTGGCGATCTTCAATACCTTGCGGATTTTGTCCTCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATAAtgt                                                                                                                                             <  2:1875302/150‑1 (MQ=255)
           gCTCTTTGGCGATCTTCAATACCTTGCGGATTTTGTCCTCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCa                                                                                                                                          >  1:6302968/1‑150 (MQ=255)
                  ggCGATCTTCAATACCTTGCGGATTTTGTCCTCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAAt                                                                                                                                   >  1:1833537/1‑150 (MQ=255)
                    cGATCTTCAATACCTTGCGGATTTTGTCCTCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGc                                                                                                                                 <  1:6199397/150‑1 (MQ=255)
                     gATCTTCAATACCTTGCGGATTTTGTCCTCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCg                                                                                                                                >  1:3834951/1‑150 (MQ=255)
                     gATCTTCAATACCTTGCGGATTTTGTCCTCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCg                                                                                                                                >  1:4651840/1‑150 (MQ=255)
                     gATCTTCAATACCTTGCGGATTTTGTCCTCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCg                                                                                                                                >  2:7361298/1‑150 (MQ=255)
                         ttCAATACCTTGCGGATTTTGTCCTCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATg                                                                                                                            >  2:2652130/1‑150 (MQ=255)
                           cAATACCTTGCGGATTTTGTCCTCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGgt                                                                                                                          <  1:3181558/150‑1 (MQ=255)
                           cAATACCTTGCGGATTTTGTCCTCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGgt                                                                                                                          >  1:1618177/1‑150 (MQ=255)
                               aCCTTGCGGATTTTGTCCTCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGgtgcgt                                                                                                                      <  2:201124/150‑1 (MQ=255)
                               aCCTTGCGGATTTTGTCCTCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGgtgcgt                                                                                                                      <  2:9137831/150‑1 (MQ=255)
                                 cTTGCGGATTTTGTCCTCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGc                                                                                                                                 <  2:8721172/137‑1 (MQ=255)
                                  ttGCGGATTTTGTCCTCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGcc                                                                                                                   >  1:1168357/1‑150 (MQ=255)
                                  ttGCGGATTTTGTCCTCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGcc                                                                                                                   >  2:3722815/1‑150 (MQ=255)
                                  ttGCGGATTTTGTCCTCAGGCATTTCCATGCGCTCGACCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGcc                                                                                                                   >  2:7962051/1‑150 (MQ=255)
                                   tGCGGATTTTGTCCTCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCt                                                                                                                  <  2:2380902/150‑1 (MQ=255)
                                    gCGGATTTTGTCCTCAGGCATTTCCATGCGCTAGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTg                                                                                                                 >  2:1410139/1‑150 (MQ=255)
                                       gATTTTGTCCTCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGACTGCGGATGGTGCGTGCCTGGTc                                                                                                              <  2:3250598/150‑1 (MQ=255)
                                            tGTCCACAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGa                                                                                                         <  1:8325586/150‑1 (MQ=255)
                                              tCCTCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATc                                                                                                       <  2:4697004/150‑1 (MQ=255)
                                                 tCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATc                                                                                                       <  1:6560172/147‑1 (MQ=255)
                                                 tCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGaa                                                                                                    >  2:8360460/1‑150 (MQ=255)
                                                 tCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGaa                                                                                                    >  2:8545985/1‑150 (MQ=255)
                                                 tCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGaa                                                                                                    >  2:2157556/1‑150 (MQ=255)
                                                 tCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTACTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGaa                                                                                                    >  1:1068016/1‑150 (MQ=255)
                                                  cAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAAc                                                                                                   <  1:1485821/150‑1 (MQ=255)
                                                   agacATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACg                                                                                                  >  1:7708993/4‑150 (MQ=255)
                                                   aGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACg                                                                                                  >  2:5934154/1‑150 (MQ=255)
                                                     gCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACggg                                                                                                >  1:3049106/1‑150 (MQ=255)
                                                     gCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACggg                                                                                                <  2:7625071/150‑1 (MQ=255)
                                                      cATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGt                                                                                               <  1:8561250/150‑1 (MQ=255)
                                                          tCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATc                                                                                           <  2:8000455/150‑1 (MQ=255)
                                                             aTGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGcc                                                                                        <  2:1599653/150‑1 (MQ=255)
                                                             aTGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGcc                                                                                        >  1:7239583/1‑150 (MQ=255)
                                                              tGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCt                                                                                       >  2:5446256/1‑150 (MQ=255)
                                                                 gcTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGAc                                                                                    <  1:2671401/150‑1 (MQ=255)
                                                                  cTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACg                                                                                   <  1:4825929/150‑1 (MQ=255)
                                                                   tCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGa                                                                                     <  2:3023022/147‑1 (MQ=255)
                                                                   tCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACgg                                                                                  >  1:1375024/1‑150 (MQ=255)
                                                                     gCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGAt                                                                                >  2:8656778/1‑150 (MQ=255)
                                                                      gccAGTTCTTCCGGCGTTGGTTCGCGACCGATTTCATGCAGCATCTGGCGCGATGTACGAACGATCTTGTTGATCGTTTCGATCATGTGCACCGGAATACGGATGGTGCGCGCCTGATCGGCAATCGAACGGGTGATTGCCTGACGAATc                                                                               <  2:4303712/149‑1 (MQ=255)
                                                                       ccAGTTCTTCCGGCGTTGGTTCGCGACCGATTTCATGCAGCATCTGGCGCGATGTACGAACGATCTTGTTGATCGTTTCGATCATGTGCACCGGAATACGGa                                                                                                                              <  1:8394851/102‑1 (MQ=255)
                                                                       ccAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATcc                                                                              <  1:8211467/150‑1 (MQ=255)
                                                                        cAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATcca                                                                             >  1:6436826/1‑150 (MQ=255)
                                                                         aGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATccac                                                                            >  1:1902412/1‑150 (MQ=255)
                                                                         aGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATccac                                                                            >  1:3253789/1‑150 (MQ=255)
                                                                           ctctTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCCTCTGGCGGGAACTACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATccacca                                                                          <  1:5340217/150‑1 (MQ=255)
                                                                            tctTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAg                                                                         >  1:8639532/1‑150 (MQ=255)
                                                                              ttCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGt                                                                       >  1:511382/1‑150 (MQ=255)
                                                                              ttCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGt                                                                       >  1:2198320/1‑150 (MQ=255)
                                                                                ccGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTgg                                                                     <  1:2572345/150‑1 (MQ=255)
                                                                                ccGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTgg                                                                     <  2:6945491/150‑1 (MQ=255)
                                                                                  ggAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCa                                                                   >  2:4136863/1‑150 (MQ=255)
                                                                                     gTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAg                                                                <  2:8893515/150‑1 (MQ=255)
                                                                                        ggTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTc                                                             <  2:8012522/150‑1 (MQ=255)
                                                                                        ggTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTc                                                             <  2:2918139/150‑1 (MQ=255)
                                                                                         gTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCg                                                            >  2:5086466/1‑150 (MQ=255)
                                                                                         gTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCg                                                            >  1:8112404/1‑150 (MQ=255)
                                                                                          ttCGCGACCGATTTCATGCAGCATCTGGCGCGATGTACGAACGATCTTGTTGATCGTTTCGATCATGTGCACCGGAATACGGATGGTGCGCGCCTGATCGGCAATCGAACGGGTGATTGCCTGACGAATCCACCATGTCGCATAGGTCga                                                           <  1:5512133/150‑1 (MQ=255)
                                                                                          ttCGCGACCGATTTCATGCAGCATCTGGCGCGATGTACGAACGATCTTGTTGATCGTTTCGATCATGTGCACCGGAATACGGATGGTGCGCGCCTGATCGGCAATCGAACGGGTGATTGCCTGACGAATCCACCATGTCGCATAGGTCga                                                           <  1:156799/150‑1 (MQ=255)
                                                                                          ttCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCga                                                           >  1:8761422/1‑150 (MQ=255)
                                                                                            cgcgACCGATTTCATGCAGCATCTGGCGCGATGTACGAACGATCTTGTTGATCGTTTCGATCATGTGCACCGGAATACGGATGGTGCGCGCCTGATCGGCAATCGAACGGGTGATTGCCTGACGAATCCACCATGTCGCATAGGTCgaga                                                         >  2:3880965/1‑150 (MQ=255)
                                                                                            cgcgACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCgaga                                                         <  2:576711/150‑1 (MQ=255)
                                                                                             gcgACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCGAGaa                                                        <  1:9037674/150‑1 (MQ=255)
                                                                                             gcgACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCGAGaa                                                        <  2:6651357/150‑1 (MQ=255)
                                                                                             gcgACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCGAGaa                                                        <  1:4436101/150‑1 (MQ=255)
                                                                                             acgACCGATTTCATGCAGCATCTGGCGCGATGTACGAACGATCTTGTTGATCGTTTCGATCATGTGCACCGGAATACGGATGGTGCGCGCCTGATCGGCAATCGAACGGGTGATTGCCTGACGAATCCACCATGTCGCATAGGTCGAGaa                                                        <  1:4989106/149‑1 (MQ=255)
                                                                                                  ccATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCGAGAACTTGt                                                   >  1:5604441/1‑150 (MQ=255)
                                                                                                   cATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCGAGAACTTGTa                                                  <  1:2765663/150‑1 (MQ=255)
                                                                                                     tttCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCGAGAACTTGTAGc                                                >  2:6554399/1‑150 (MQ=255)
                                                                                                       tCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCGAGAACTTGTAGCcg                                              >  1:2397588/1‑150 (MQ=255)
                                                                                                          tGCAGCATCTGGCGCGATGTACGAACGATCTTGTTGATCGTTTCGATCATGTGCACCGGAATACGGATGGTGCGCGCCTGATCGGCAATCGAACGGGTGATTGCCTGACGAATCCACCATGTCGCATAGGTCGAGAACTTGTAACCACGa                                           >  1:3728804/1‑150 (MQ=255)
                                                                                                            cagcaTCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCGAGAACTTGTAGCCGCGACg                                         >  2:2341752/1‑150 (MQ=255)
                                                                                                            cagcaTCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCGAGAACTTGTAGCCGCGACg                                         >  2:1751430/1‑150 (MQ=255)
                                                                                                              gcaTCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGt                                       >  1:7583336/1‑150 (MQ=255)
                                                                                                               caTCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTa                                      <  2:7313847/150‑1 (MQ=255)
                                                                                                                aTCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTAt                                     <  2:3816941/150‑1 (MQ=255)
                                                                                                                  cTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTc                                   <  2:4452525/150‑1 (MQ=255)
                                                                                                                  cTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTc                                   <  2:7670378/150‑1 (MQ=255)
                                                                                                                           aaTACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTc                          <  2:6171593/150‑1 (MQ=255)
                                                                                                                                 aacgatcTTGTTGATCGTTTCGATCATGTGCACCGGAATACGGATGGTGCGCGCCTGATCGGCAATCGAACGGGTGATTGCCTGACGAATCCACCATGTCGCATAGGTCGAGAACTTGTAACCACGACGATATTCGAACTTATCGACCGc                    >  2:5988848/7‑150 (MQ=255)
                                                                                                                                  acgatcTTGTTGATCGTTTCGATCATGTGCACCGGAATACGGATGGTGCGCGCCTGATCGGCAATCGAACGGGTGATTGCCTGACGAATCCACCATGTCGCATAGGTCGAGAACTTGTAACCACGACGATATTCGAACTTATCGACCGcc                   <  2:4628434/145‑2 (MQ=255)
                                                                                                                                    gAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCttt                 >  1:3622020/1‑150 (MQ=255)
                                                                                                                                     aGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTtc                <  1:5873266/150‑1 (MQ=255)
                                                                                                                                        ttgttgATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCGCCGCTTtcatc             <  1:2966031/150‑1 (MQ=255)
                                                                                                                                           ttgATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGa          >  1:2028350/1‑150 (MQ=255)
                                                                                                                                            tgATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCGAGGACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGAc         <  2:2497174/150‑1 (MQ=255)
                                                                                                                                             gATCGTCTCGATCATGTGCACCGGAATGCGGCTGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGAcc        <  1:423089/150‑1 (MQ=255)
                                                                                                                                             gATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGAcc        <  1:3475457/150‑1 (MQ=255)
                                                                                                                                               tCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGa      >  1:4026790/1‑150 (MQ=255)
                                                                                                                                                 gTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATg    >  1:1701654/1‑150 (MQ=255)
                                                                                                                                                   ctcGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGtt  <  1:2148403/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CATGGAGATCGGCTCTTTGGCGATCTTCAATACCTTGCGGATTTTGTCCTCAGGCATTTCCATGCGCTCGCCCAGCTCTTCCGGAGTCGGTTCGCGACCCATTTCCTGCAGCATCTGGCGGGAAATACGGTTGAGCTTGTTGATCGTCTCGATCATGTGCACCGGAATGCGGATGGTGCGTGCCTGGTCGGCGATCGAACGGGTGATCGCCTGACGGATCCACCAGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTT  >  NC_002947/471408‑471704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑