BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,992,336	C→G	9.0%	A103A (GCG→GCC)	ycaC‑III ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,992,336	0	C	G	9.0%	1234.2 / 84.4	379	A103A (GCG→GCC)	ycaC‑III	putative hydrolase
Reads supporting (aligned to +/- strand): ref base C (209/136); new base G (20/14); total (229/150)
Fisher's exact test for biased strand distribution p-value = 8.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01

Only 100 of 380 total aligned reads displayed.

TGCGCGGCATCACGGGTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCG  >  NC_002947/5992187‑5992484

tGCGCGGCATCACGGGTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTc                                                                                                                                                      >  1:4539442/1‑150 (MQ=255)

 gcgcGGCATCACGGGTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCg                                                                                                                                                     >  1:1120508/1‑150 (MQ=255)

 gcgcGGCATCACGGGTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCg                                                                                                                                                     >  1:2023721/1‑150 (MQ=255)

  cgcgGCATCACGGGTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGc                                                                                                                                                    <  1:2218068/150‑1 (MQ=255)

   gcgGCATCACGGGTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGcc                                                                                                                                                   >  2:1995893/1‑150 (MQ=255)

   gaggCATCACGGGTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCATACCTCGGTCACAACACCTGCAATGATCAGCTTCTTCTTGCCGGTCGcc                                                                                                                                                   <  1:8857295/148‑1 (MQ=255)

           aCGGGTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGc                                                                                                                                           >  2:3467338/1‑150 (MQ=255)

               gTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTg                                                                                                                                       <  1:2684793/150‑1 (MQ=255)

                   tttcattGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGa                                                                                                                                   >  1:9152704/1‑150 (MQ=255)

                     tcattGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGaaa                                                                                                                                 <  2:2954946/150‑1 (MQ=255)

                       attGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATc                                                                                                                               >  1:6113810/1‑150 (MQ=255)

                          gAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTc                                                                                                                            >  2:5110605/1‑150 (MQ=255)

                          gAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTc                                                                                                                            <  2:4310604/150‑1 (MQ=255)

                              gTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCAGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCATGACGAAATCCTCGTTg                                                                                                                        >  1:5362190/1‑150 (MQ=255)

                                    gAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAg                                                                                                                  >  2:6646911/1‑150 (MQ=255)

                                    gAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAg                                                                                                                  <  2:8904280/150‑1 (MQ=255)

                                         aacGGTCACCACGAACACCACAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGtt                                                                                                             <  1:7926273/148‑1 (MQ=255)

                                         aTCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCggt                                                                                                             >  2:1595383/1‑148 (MQ=255)

                                         aTCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGtt                                                                                                             >  2:1682369/1‑150 (MQ=255)

                                          tCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTg                                                                                                            >  2:8316988/1‑150 (MQ=255)

                                           cGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGa                                                                                                           <  1:1050486/150‑1 (MQ=255)

                                             gTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATc                                                                                                         >  1:1795336/1‑150 (MQ=255)

                                               caccacGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGGCGAAATCCTCGTTGTCCCAGGCGTTGATCTg                                                                                                       <  1:3685843/150‑1 (MQ=255)

                                                     gAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAgg                                                                                                 >  1:8275009/1‑150 (MQ=255)

                                                       acacCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAgggc                                                                                               >  1:6241266/1‑150 (MQ=255)

                                                         acCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAgggcgg                                                                                             <  1:5589166/150‑1 (MQ=255)

                                                          ccTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAgggcggg                                                                                            >  2:1868839/1‑150 (MQ=255)

                                                           cTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAgggcgggc                                                                                           >  2:7046141/1‑150 (MQ=255)

                                                           cTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAgggcgggc                                                                                           >  2:6740586/1‑150 (MQ=255)

                                                            tCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCACACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCa                                                                                          >  1:7599093/1‑150 (MQ=255)

                                                            tCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCa                                                                                          >  1:4688603/1‑150 (MQ=255)

                                                            tCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCa                                                                                          >  1:3295636/1‑150 (MQ=255)

                                                                  tcttcCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTAc                                                                                    <  1:2829692/150‑1 (MQ=255)

                                                                    ttcCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACgg                                                                                  >  1:8425181/1‑150 (MQ=255)

                                                                       ctccAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGgcgc                                                                               >  2:3276073/1‑150 (MQ=255)

                                                                       ctccAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGgcgc                                                                               >  1:986054/1‑150 (MQ=255)

                                                                        tccAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCa                                                                              >  2:4115535/1‑150 (MQ=255)

                                                                         ccAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCAt                                                                             >  2:7836955/1‑150 (MQ=255)

                                                                          cAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATc                                                                            >  2:6108007/1‑150 (MQ=255)

                                                                          cAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATc                                                                            >  2:5630594/1‑150 (MQ=255)

                                                                                 gACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAAc                                                                     >  1:7387101/1‑150 (MQ=255)

                                                                                    aGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAgg                                                                  <  1:1506157/150‑1 (MQ=255)

                                                                                      tgcCGGGAAAGCCACGCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGaatgtttc                                                                >  2:1372450/2‑144 (MQ=14)

                                                                                      cgcCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGc                                                                <  1:7458451/150‑1 (MQ=255)

                                                                                      cgcCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGc                                                                <  1:755705/150‑1 (MQ=255)

                                                                                        ccGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCtt                                                              <  1:5889407/150‑1 (MQ=255)

                                                                                         cGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCttt                                                             <  2:2619393/150‑1 (MQ=255)

                                                                                           ggAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCa                                                           >  1:6540646/1‑150 (MQ=255)

                                                                                               cGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTc                                                       <  1:1933182/150‑1 (MQ=255)

                                                                                                  cACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCgg                                                    >  2:37018/1‑150 (MQ=255)

                                                                                                   aCGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGc                                                   >  2:6169721/1‑150 (MQ=255)

                                                                                                     gCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCAc                                                 <  1:8591899/150‑1 (MQ=9)

                                                                                                     gCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCAc                                                 >  2:4611421/1‑150 (MQ=9)

                                                                                                     gCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCAc                                                 <  1:1566163/150‑1 (MQ=255)

                                                                                                       aaaCCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCa                                               >  1:3968986/1‑150 (MQ=255)

                                                                                                       aaaCCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCa                                               >  1:4051730/1‑150 (MQ=255)

                                                                                                       aaaCCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCa                                               >  2:7227516/1‑150 (MQ=255)

                                                                                                          cttCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCaatg                                            <  2:8137616/148‑4 (MQ=9)

                                                                                                            tCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCggg                                          >  2:4166556/1‑150 (MQ=255)

                                                                                                            tCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCggg                                          >  2:2711738/1‑150 (MQ=255)

                                                                                                            tCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCggg                                          >  1:3901745/1‑150 (MQ=255)

                                                                                                             cGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGc                                         <  2:1511951/150‑1 (MQ=9)

                                                                                                              ggTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGcc                                        >  1:6457833/1‑150 (MQ=255)

                                                                                                               gTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGAGCTGGCCAGTGCGGGCAATGTACGGCGCATCCGGGACCAGGGCTTTCAGTTCCGGCACCAGCGGGCCg                                       >  2:7510511/1‑150 (MQ=255)

                                                                                                               gTCACAACACCTGCAATGATCAGCTGCGTCTTGCCGGTCGCCGTCCCCGCCTTGACGAAATCCTCGGTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCg                                       <  2:761485/150‑1 (MQ=255)

                                                                                                                  acaacaCCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCAttc                                    <  2:2323280/150‑2 (MQ=2)

                                                                                                                  acaacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTg                                    <  2:5370899/150‑1 (MQ=255)

                                                                                                                  acaacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTg                                    >  2:6974149/1‑150 (MQ=255)

                                                                                                                   caacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTgg                                   >  1:8093054/1‑150 (MQ=255)

                                                                                                                   caacaCCAGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTgg                                   <  1:5090160/150‑1 (MQ=255)

                                                                                                                    aacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTggg                                  >  1:1222623/1‑150 (MQ=255)

                                                                                                                    aacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTggg                                  <  1:7073403/150‑1 (MQ=255)

                                                                                                                    aacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTggg                                  >  1:5491882/1‑150 (MQ=255)

                                                                                                                    aacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTggg                                  >  1:692079/1‑150 (MQ=255)

                                                                                                                           gCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGc                           >  2:4863673/1‑150 (MQ=255)

                                                                                                                           gCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGc                           <  2:570682/150‑1 (MQ=255)

                                                                                                                           gCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGc                           <  1:4477822/150‑1 (MQ=255)

                                                                                                                            cAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCt                          >  2:8788003/1‑150 (MQ=255)

                                                                                                                             aaTGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCACTGTACGGCGCATCCGGGAACAGGGCTGTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTc                         >  2:9012743/1‑150 (MQ=255)

                                                                                                                             aaTGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTc                         >  1:4640926/1‑150 (MQ=255)

                                                                                                                                gATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGaa                      <  1:6646911/150‑1 (MQ=255)

                                                                                                                                   cAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCt                   <  1:6323773/150‑1 (MQ=255)

                                                                                                                                   cAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCt                   >  2:7310755/1‑150 (MQ=255)

                                                                                                                                   cAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCt                   <  1:3467338/150‑1 (MQ=255)

                                                                                                                                     gctgctTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCtgg                 >  1:3060364/1‑150 (MQ=255)

                                                                                                                                     gctgctTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCtgg                 >  2:2448074/1‑150 (MQ=255)

                                                                                                                                      ctgctTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACtggt                <  2:8657704/150‑1 (MQ=2)

                                                                                                                                       tgctTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACtggtg               >  2:6240309/1‑150 (MQ=2)

                                                                                                                                       tgctTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCtggtg               <  1:2356479/150‑1 (MQ=255)

                                                                                                                                       tgctTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCtggtg               <  2:3897171/150‑1 (MQ=255)

                                                                                                                                         cttcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTggtggt             >  1:7254236/1‑150 (MQ=255)

                                                                                                                                          ttcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGgtggtg            >  2:226758/1‑150 (MQ=255)

                                                                                                                                            cttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAg          <  1:5633056/150‑1 (MQ=255)

                                                                                                                                              tGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGa        <  2:246915/150‑1 (MQ=255)

                                                                                                                                               gCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGAt       <  2:1083690/150‑1 (MQ=255)

                                                                                                                                                ccGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATg      >  2:6472469/1‑150 (MQ=255)

                                                                                                                                                 cGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATgg     >  2:7682695/1‑150 (MQ=255)

                                                                                                                                                 cGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATgg     >  2:9133691/1‑150 (MQ=255)

                                                                                                                                                  ggTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGt    >  2:3211515/1‑150 (MQ=255)

                                                                                                                                                  ggTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAACGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGt    <  2:1120508/150‑1 (MQ=255)

TGCGCGGCATCACGGGTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCG  >  NC_002947/5992187‑5992484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`