BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,992,345	C→T	8.8%	A100A (GCG→GCA)	ycaC‑III ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,992,345	0	C	T	8.8%	1216.7 / 70.3	385	A100A (GCG→GCA)	ycaC‑III	putative hydrolase
Reads supporting (aligned to +/- strand): ref base C (213/138); new base T (20/14); total (233/152)
Fisher's exact test for biased strand distribution p-value = 8.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

Only 100 of 393 total aligned reads displayed.

ACGGGTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAA  >  NC_002947/5992198‑5992494

aCGGGTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGc                                                                                                                                                     >  2:6323773/1‑150 (MQ=255)

 cGGGTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGcc                                                                                                                                                    >  2:8212939/1‑150 (MQ=255)

 cGGGTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGcc                                                                                                                                                    >  2:2278845/1‑150 (MQ=255)

     tcatttcattGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGa                                                                                                                                                <  1:8949839/150‑1 (MQ=255)

       atttcattGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACg                                                                                                                                              >  1:5897324/1‑150 (MQ=255)

        tttcattGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGa                                                                                                                                             >  1:8730289/1‑150 (MQ=255)

            attGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATc                                                                                                                                         >  1:6113810/1‑150 (MQ=255)

                aaGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCg                                                                                                                                     <  2:862410/150‑1 (MQ=255)

                        ggAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGACACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCATCGTTGTCCCa                                                                                                                             >  1:3010092/1‑150 (MQ=255)

                        ggAAGCATCGGTCACCACGAACACCTCAAATTCGTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCa                                                                                                                             <  2:7956828/150‑1 (MQ=255)

                             cATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGt                                                                                                                        >  2:1590173/1‑150 (MQ=255)

                              aacGGTCACCACGAACACCACAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGtt                                                                                                                       <  1:7926273/148‑1 (MQ=255)

                              aTCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGtt                                                                                                                       <  2:7076574/150‑1 (MQ=255)

                              aTCGGTCACCACGAACAACTCAAATACTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCCGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCATTGACGAAATCCTCGTTGTCCCAGGCGtt                                                                                                                       <  2:6208545/150‑1 (MQ=255)

                                cGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGa                                                                                                                     <  1:1050486/150‑1 (MQ=255)

                                  gTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATc                                                                                                                   >  1:1130253/1‑150 (MQ=255)

                                  gTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATc                                                                                                                   >  1:1795336/1‑150 (MQ=255)

                                   tCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCt                                                                                                                  >  2:4122773/1‑150 (MQ=255)

                                      ccacGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGc                                                                                                               <  1:5370302/150‑1 (MQ=255)

                                       cacGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGt                                                                                                                                    <  2:752774/128‑1 (MQ=255)

                                       cacGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGt                                                                                                                                    >  1:752774/1‑128 (MQ=255)

                                         cGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAg                                                                                                            <  2:7634591/150‑1 (MQ=255)

                                                cTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAgggcgggc                                                                                                     >  2:6740606/1‑150 (MQ=255)

                                                 tCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCTACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCa                                                                                                    >  1:3809690/1‑150 (MQ=255)

                                                 tCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCACACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCa                                                                                                    >  1:7599093/1‑150 (MQ=255)

                                                 tCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCa                                                                                                    >  1:4688603/1‑150 (MQ=255)

                                                 tCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCa                                                                                                    >  1:3295634/1‑150 (MQ=255)

                                                 tCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCa                                                                                                    >  1:4320267/1‑150 (MQ=255)

                                                 tCAAATTCTTCCACCAAGGCCGACAGCCACGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCATCTGCTTCTTGCCTGTCGCCTTCACCGCATTGACGAAATCAACGATTTCCCAGGCTATGATCTGGACAGTGCGGGCa                                                                                                    >  2:1109556/1‑150 (MQ=255)

                                                  cAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCaa                                                                                                   >  1:5595762/1‑150 (MQ=255)

                                                  cAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCaa                                                                                                   >  1:1986680/1‑150 (MQ=255)

                                                   aaaTTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAAt                                                                                                  <  2:1986680/150‑1 (MQ=255)

                                                       tcttcCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTAc                                                                                              <  1:2829686/150‑1 (MQ=255)

                                                         ttcCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACgg                                                                                            >  2:4979058/1‑150 (MQ=255)

                                                         ttcCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACgg                                                                                            >  2:405543/1‑150 (MQ=255)

                                                            ctccAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGgcgc                                                                                         >  2:3276073/1‑150 (MQ=255)

                                                               cAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATc                                                                                      >  2:6108007/1‑150 (MQ=255)

                                                               cAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATc                                                                                      >  2:5630594/1‑150 (MQ=255)

                                                                  gcccGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCgg                                                                                   <  1:2107136/148‑1 (MQ=255)

                                                                   gCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCCCTGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTGGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCggg                                                                                  >  2:285247/1‑150 (MQ=255)

                                                                   gCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCggg                                                                                  >  1:6917076/1‑150 (MQ=255)

                                                                     ggacaatgcCGGGAAAGCCACGCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGaa                                                                                >  2:1033417/8‑150 (MQ=14)

                                                                      gACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAAc                                                                               >  1:7375244/1‑150 (MQ=255)

                                                                       aCAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACa                                                                              <  1:151082/150‑1 (MQ=255)

                                                                         aGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGGCGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAgg                                                                            <  2:4833423/150‑1 (MQ=255)

                                                                           cgcCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGc                                                                          <  1:7457703/150‑1 (MQ=255)

                                                                               gggAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTc                                                                      <  1:4525845/150‑1 (MQ=255)

                                                                                ggAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCa                                                                     <  2:7442173/150‑1 (MQ=255)

                                                                                ggAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCa                                                                     <  2:356101/150‑1 (MQ=255)

                                                                                ggAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCa                                                                     <  2:7759591/150‑1 (MQ=255)

                                                                                ggAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCa                                                                     >  1:6540646/1‑150 (MQ=255)

                                                                                    cGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTc                                                                 <  1:2327532/150‑1 (MQ=255)

                                                                                     gCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTcc                                                                >  2:131231/1‑150 (MQ=255)

                                                                                     gCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTcc                                                                >  2:6648070/1‑150 (MQ=255)

                                                                                       cACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCGTTCAGTTCCgg                                                              >  2:8772735/1‑150 (MQ=255)

                                                                                         cGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCa                                                            >  2:660102/1‑150 (MQ=255)

                                                                                          gCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCAc                                                           >  2:4611421/1‑150 (MQ=9)

                                                                                          gCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCAc                                                           <  1:1566163/150‑1 (MQ=255)

                                                                                            aaaCCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCa                                                         >  2:7227516/1‑150 (MQ=255)

                                                                                              aCCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGc                                                       >  1:263800/1‑150 (MQ=255)

                                                                                                   ggTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGcc                                                  >  1:6457833/1‑150 (MQ=255)

                                                                                                    gTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCACTGTACGGCGCATCCGGGAACAGGGCTGTCAGTTCCGGCACCAGCGGGCCg                                                 >  1:3950826/1‑150 (MQ=255)

                                                                                                       acaacaCCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCAttc                                              >  1:830551/1‑149 (MQ=2)

                                                                                                       acaacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTg                                              <  2:5370899/150‑1 (MQ=255)

                                                                                                       acaacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTg                                              >  2:8822862/1‑150 (MQ=255)

                                                                                                       acaacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTg                                              >  2:6974557/1‑150 (MQ=255)

                                                                                                        caacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTgg                                             >  2:3208418/1‑150 (MQ=255)

                                                                                                         aacaCCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTcgg                                            >  1:5655282/1‑150 (MQ=2)

                                                                                                         aacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTggg                                            >  1:5491882/1‑150 (MQ=255)

                                                                                                         aacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTggg                                            >  1:8176722/1‑150 (MQ=255)

                                                                                                         aacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTggg                                            >  2:4317169/1‑150 (MQ=255)

                                                                                                         aacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTggg                                            >  1:922333/1‑150 (MQ=255)

                                                                                                         aacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTggg                                            <  1:7073403/150‑1 (MQ=255)

                                                                                                         aacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTggg                                            >  2:1449256/1‑150 (MQ=255)

                                                                                                              cTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGccgt                                       >  2:1780673/1‑148 (MQ=2)

                                                                                                                gCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGc                                     <  2:570682/150‑1 (MQ=255)

                                                                                                                  aaTGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCACTGTACGGCGCATCCGGGAACAGGGCTGTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTc                                   >  2:9012743/1‑150 (MQ=255)

                                                                                                                  aaTGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTc                                   >  1:4640926/1‑150 (MQ=255)

                                                                                                                     gATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGaa                                <  1:6646911/150‑1 (MQ=255)

                                                                                                                       tCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGc                              >  1:4000746/1‑150 (MQ=255)

                                                                                                                        cAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCt                             <  1:6323773/150‑1 (MQ=255)

                                                                                                                        cAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCt                             >  2:7310755/1‑150 (MQ=255)

                                                                                                                          gctgctTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCtgg                           >  2:2448074/1‑150 (MQ=255)

                                                                                                                           ctgctTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACtggt                          <  1:5142305/150‑1 (MQ=2)

                                                                                                                           ctgctTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACtggt                          <  1:1236478/150‑1 (MQ=2)

                                                                                                                            tgctTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACtggtg                         <  2:4300562/150‑1 (MQ=2)

                                                                                                                            tgctTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCtggtg                         <  1:79438/150‑1 (MQ=255)

                                                                                                                            tgctTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCtggtg                         <  1:3402304/150‑1 (MQ=255)

                                                                                                                              cttcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTggtggt                       >  1:7253905/1‑150 (MQ=255)

                                                                                                                                tcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGa                     <  1:8504840/150‑1 (MQ=255)

                                                                                                                                 cttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAg                    >  2:165482/1‑150 (MQ=255)

                                                                                                                                   tGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGa                  <  2:247006/150‑1 (MQ=255)

                                                                                                                                     ccGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGGGAGGATg                >  2:3767642/1‑150 (MQ=255)

                                                                                                                                      cGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATgg               >  1:6219064/1‑150 (MQ=255)

                                                                                                                                      cGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATgg               >  2:9133691/1‑150 (MQ=255)

                                                                                                                                       ggTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGt              <  1:3616052/150‑1 (MQ=255)

                                                                                                                                            ccTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCa         <  2:2704960/150‑1 (MQ=255)

                                                                                                                                              ttCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAgg       >  1:4868041/1‑150 (MQ=255)

                                                                                                                                              ttCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAgg       <  2:2630422/150‑1 (MQ=255)

                                                                                                                                                  ccGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGACGCTGGTGGTGAGGATGGTCGGCAGGTTga   <  2:2193883/150‑1 (MQ=255)

ACGGGTCATTTCATTGAAGGTGCCGGAAGCATCGGTCACCACGAACACCTCAAATTCTTCCTCCAAGGCCGACAGCGCCGGGAACGCCACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAA  >  NC_002947/5992198‑5992494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`