BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,992,440	G→A	6.1%	L69L (CTG→TTG)	ycaC‑III ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,992,440	0	G	A	6.1%	1230.2 / 41.7	373	L69L (CTG→TTG)	ycaC‑III	putative hydrolase
Reads supporting (aligned to +/- strand): ref base G (178/172); new base A (12/11); total (190/183)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 375 total aligned reads displayed.

AACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATCGCGCACCAGCGACAGCAGGCCAGCCTGGTGGTCAA  >  NC_002947/5992291‑5992589

 aaagacgacgctgccgtactgctggttgaccaccaggctggcctgctgtcgctggtgcgcgatatcgagccagacaagttcaagaacaacgtgctggcgctggctgacctggccaagttcttcaacctgccgaccatcctcacCACCAGc                                                                                                                                                      <  2:4930942‑M1/7‑1 (MQ=255)

 aCCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGc                                                                                                                                                      >  1:263800/1‑150 (MQ=255)

   cTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCgg                                                                                                                                                    <  1:6034614/150‑1 (MQ=255)

   cTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCgg                                                                                                                                                    <  2:8146547/150‑1 (MQ=255)

    tCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCggg                                                                                                                                                   >  1:3901745/1‑150 (MQ=255)

    tCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCggg                                                                                                                                                   >  2:2711738/1‑150 (MQ=255)

     cGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGc                                                                                                                                                  <  2:1511951/150‑1 (MQ=9)

       gTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGAGCTGGCCAGTGCGGGCAATGTACGGCGCATCCGGGACCAGGGCTTTCAGTTCCGGCACCAGCGGGCCg                                                                                                                                                >  2:7510511/1‑150 (MQ=255)

          acaacaCCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCAttc                                                                                                                                             <  2:2323280/150‑2 (MQ=2)

          acaacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTg                                                                                                                                             >  2:8822862/1‑150 (MQ=255)

          acaacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTg                                                                                                                                             >  2:6974149/1‑150 (MQ=255)

           caacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTgg                                                                                                                                            >  2:3208418/1‑150 (MQ=255)

            aacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTggg                                                                                                                                           >  2:1449256/1‑150 (MQ=255)

            aacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTggg                                                                                                                                           >  1:8176722/1‑150 (MQ=255)

            aacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTggg                                                                                                                                           >  1:5491882/1‑150 (MQ=255)

                 cTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGccgt                                                                                                                                      >  2:1780673/1‑148 (MQ=2)

                   gcgaTGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGccgttt                                                                                                                                    <  1:4895631/147‑5 (MQ=2)

                   gcgaTGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGccgttt                                                                                                                                    >  2:5650462/4‑146 (MQ=2)

                   gCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGc                                                                                                                                    <  2:570682/150‑1 (MQ=255)

                          tCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGc                                                                                                                             >  1:4000746/1‑150 (MQ=255)

                            aGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCtg                                                                                                                           <  1:2993037/150‑1 (MQ=255)

                             gctgctTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCtgg                                                                                                                          >  1:4382307/1‑150 (MQ=255)

                              ctgctTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGTTGATCTGGCCGGGGCGAGCGATATAAGGCGCATCCGGGAATGTTTCTTTCAGCTCCGGCACCAATGGGCCATTCGGGCCGTTTTCAAAACtggt                                                                                                                         <  2:8657704/150‑1 (MQ=2)

                               tgctTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCtggtg                                                                                                                        >  1:1582073/1‑150 (MQ=255)

                               caccaggctggcctgctgtcgctggtgcgcgatatcgagccagacaagttcaagaacaacgtgctggcgctggctgacctggccaagttcttcaacctgccgaccatcctcacCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCtggtg                                                                                                                        >  1:6751994‑M1/114‑150 (MQ=255)

                                 cttcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTggtggt                                                                                                                      >  1:7254236/1‑150 (MQ=255)

                                  ttcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGgtggtg                                                                                                                     >  1:4582058/1‑150 (MQ=255)

                                  ttcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGgtggtg                                                                                                                     >  1:3275546/1‑150 (MQ=255)

                                  ttcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGgtggtg                                                                                                                     >  2:226758/1‑150 (MQ=255)

                                   tcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGa                                                                                                                    <  1:8504840/150‑1 (MQ=255)

                                   tcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGa                                                                                                                    <  2:6623920/150‑1 (MQ=255)

                                   tcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGa                                                                                                                    <  1:2181752/150‑1 (MQ=255)

                                   tcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGa                                                                                                                    <  1:3501517/150‑1 (MQ=255)

                                    cttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAg                                                                                                                   <  1:5633056/150‑1 (MQ=255)

                                      tGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGa                                                                                                                 <  2:247006/150‑1 (MQ=255)

                                       gCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGAt                                                                                                                <  2:1083953/150‑1 (MQ=255)

                                        ccGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGGGAGGATg                                                                                                               >  2:3767642/1‑150 (MQ=255)

                                         cGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATgg                                                                                                              >  1:6219064/1‑150 (MQ=255)

                                         cGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATgg                                                                                                              >  2:7682695/1‑150 (MQ=255)

                                         cGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATgg                                                                                                              >  2:9133691/1‑150 (MQ=255)

                                              gCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGc                                                                                                         >  2:7096317/1‑150 (MQ=255)

                                               ccTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCa                                                                                                        <  2:7375244/150‑1 (MQ=255)

                                               ccTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCa                                                                                                        >  2:4517527/1‑150 (MQ=255)

                                                 ttCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAgg                                                                                                      <  1:1541274/150‑1 (MQ=255)

                                                 ttCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAgg                                                                                                      >  1:714239/1‑150 (MQ=255)

                                                  tCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGt                                                                                                     >  2:523912/1‑150 (MQ=255)

                                                    aCCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTg                                                                                                   <  1:7937848/150‑1 (MQ=255)

                                                     ccgccgTGACGAAATCCTCGTTGTCCCCGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCCTCCGGGACCAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTGGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTga                                                                                                  >  2:7595668/1‑150 (MQ=17)

                                                     ccGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGACGCTGGTGGTGAGGATGGTCGGCAGGTTga                                                                                                  <  2:2193883/150‑1 (MQ=255)

                                                     ccGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTga                                                                                                  >  1:2797852/1‑150 (MQ=255)

                                                      cGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTgaa                                                                                                 >  1:4689994/1‑150 (MQ=255)

                                                          ttGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAAc                                                                                             <  1:6401398/150‑1 (MQ=255)

                                                           tGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACt                                                                                            <  1:1002944/150‑1 (MQ=255)

                                                           tGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACt                                                                                            >  2:6897398/1‑150 (MQ=255)

                                                            gACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACtt                                                                                           <  2:5595762/150‑1 (MQ=255)

                                                             aCGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTg                                                                                          <  2:8330152/150‑1 (MQ=255)

                                                               gAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGc                                                                                        >  1:9012965/1‑150 (MQ=255)

                                                               gAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGc                                                                                        >  1:3236468/1‑150 (MQ=255)

                                                                     atCGTTGTCCCAGGCGTTGTTCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGTAGTACTTGGCCAGGTc                                                                                  >  2:4226372/2‑150 (MQ=255)

                                                                        gTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTcagc                                                                               >  1:9119779/1‑150 (MQ=255)

                                                                        gTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTcagc                                                                               >  1:7619227/1‑150 (MQ=255)

                                                                                aGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAgccagcgcca                                                                       <  1:5110605/150‑1 (MQ=255)

                                                                                       gATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACgtt                                                                <  1:4301915/150‑1 (MQ=255)

                                                                                          cTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACgttgtt                                                             <  1:1680270/150‑1 (MQ=255)

                                                                                           tGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTc                                                            <  1:6962533/150‑1 (MQ=255)

                                                                                           tGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTc                                                            <  2:6241266/150‑1 (MQ=255)

                                                                                             gCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCtt                                                          <  1:144116/150‑1 (MQ=255)

                                                                                             gCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCtt                                                          <  2:4315987/150‑1 (MQ=255)

                                                                                             gCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCtt                                                          <  1:4115535/150‑1 (MQ=255)

                                                                                                aGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGaa                                                       <  1:3411524/150‑1 (MQ=255)

                                                                                                aGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGaa                                                       <  2:6113810/150‑1 (MQ=255)

                                                                                                 gggcgggcAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAAc                                                      >  2:4023633/1‑150 (MQ=255)

                                                                                                   gcgggcAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACtt                                                    >  2:548670/1‑150 (MQ=255)

                                                                                                     gggcAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGt                                                  <  2:2946662/150‑1 (MQ=255)

                                                                                                      ggcAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTc                                                 <  1:123520/150‑1 (MQ=255)

                                                                                                      ggcAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTc                                                 <  1:8316988/150‑1 (MQ=255)

                                                                                                          aTGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGc                                             <  1:8792023/150‑1 (MQ=255)

                                                                                                           tGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCt                                            >  1:1949143/1‑150 (MQ=255)

                                                                                                              aCGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGa                                         >  1:8036808/1‑150 (MQ=255)

                                                                                                              aCGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGa                                         >  2:4801690/1‑150 (MQ=255)

                                                                                                               cGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGat                                        <  1:1595383/150‑1 (MQ=255)

                                                                                                                 gcgcATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGatat                                      >  1:7845423/1‑150 (MQ=255)

                                                                                                                     aTCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCTCGTTGTTCTTGAACTTGTCTGGCTCGATATcgcg                                  <  2:4687433/150‑1 (MQ=255)

                                                                                                                     aTCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATcgcg                                  >  2:8439205/1‑150 (MQ=255)

                                                                                                                     aTCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATcgcg                                  >  1:3816360/1‑150 (MQ=255)

                                                                                                                     aTCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATcgcg                                  >  2:7196381/1‑150 (MQ=255)

                                                                                                                      tCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATCgcgc                                 <  1:6108007/150‑1 (MQ=255)

                                                                                                                       ccGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATCGCGCa                                <  2:2021827/150‑1 (MQ=255)

                                                                                                                              cAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATCGCGCACCAGCGa                         <  1:660102/150‑1 (MQ=255)

                                                                                                                                    tttCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATCGCGCACCAGCGAcagcag                   >  1:5218076/1‑150 (MQ=255)

                                                                                                                                             cGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATCGCGCACCAGCGACAGCAGGCCAGccgg          >  1:3360259/1‑148 (MQ=255)

                                                                                                                                             cGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATCGCGCACCAGCGACAGCAGGCCAGCCtg          <  2:3295636/150‑1 (MQ=255)

                                                                                                                                              ggCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGGCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATCGCGCACCAGCGACAGCAGGCCAGCCtgg         <  1:1109556/150‑1 (MQ=255)

                                                                                                                                              ggCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGaa                                                       <  2:1014899/104‑1 (MQ=255)

                                                                                                                                              ggCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATCGCGCACCAGCGACAGCAGGCCAGCCtgg         >  2:3876281/1‑150 (MQ=255)

                                                                                                                                               gCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATCGCGCACCAGCGACAGCAGGCCAGCCtggt        <  1:2448074/150‑1 (MQ=255)

                                                                                                                                                CACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATCGCGCACCAGCGACAGCAGGCCAGCCtggtg       >  2:1222623‑M1/1‑150 (MQ=255)

                                                                                                                                                   CAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATCGCGCACCAGCGACAGCAGGCCAGCCTGGTGGTc    <  1:6481958‑M1/150‑1 (MQ=255)

                                                                                                                                                    AGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATCGCGCACCAGCGACAGCAGGCCAGCCTGGTGGTCa   <  1:7762870‑M1/150‑1 (MQ=255)

                                                                                                                                                     GCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATCGCGCACCAGCGACAGCAGGCCAGCCTGGTGGTCaa  >  2:64729‑M1/1‑150 (MQ=255)

AACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATCGCGCACCAGCGACAGCAGGCCAGCCTGGTGGTCAA  >  NC_002947/5992291‑5992589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`