Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,000,722 C→G 6.0% V134V (GTG→GTC)  ycaC‑IV ← putative hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,000,7220CG 6.0% 1088.2 / 39.4 324V134V (GTG→GTC) ycaC‑IVputative hydrolase
Reads supporting (aligned to +/- strand):  ref base C (171/133);  new base G (9/11);  total (180/144)
Fisher's exact test for biased strand distribution p-value = 3.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 328 total aligned reads displayed.

CTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCG  >  NC_002947/6000577‑6000871
                                                                                                                                                 |                                                                                                                                                     
cTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGt                                                                                                                                                   <  1:5996851/150‑1 (MQ=255)
cTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTCGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGt                                                                                                                                                   <  1:778034/150‑1 (MQ=255)
 ttCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTc                                                                                                                                                  >  1:4188119/1‑150 (MQ=255)
 ttCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTc                                                                                                                                                  >  1:3715692/1‑150 (MQ=255)
  tCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCg                                                                                                                                                 <  2:3150975/150‑1 (MQ=255)
    gATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTGGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGaa                                                                                                                                               >  2:1794437/1‑150 (MQ=255)
    gATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGaa                                                                                                                                               >  2:8071427/1‑150 (MQ=255)
     aTGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAAc                                                                                                                                              >  1:2363610/1‑150 (MQ=255)
     aTGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAAc                                                                                                                                              <  2:6989155/150‑1 (MQ=255)
       gTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAActc                                                                                                                                            <  2:4537251/150‑1 (MQ=255)
          gTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACtcttc                                                                                                                                         >  1:6323821/1‑150 (MQ=255)
          gTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACtcttc                                                                                                                                         >  1:2142047/1‑150 (MQ=255)
          gTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACtcttc                                                                                                                                         >  1:941542/1‑150 (MQ=255)
               gcCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCa                                                                                                                                    <  1:2083321/150‑1 (MQ=255)
               gcCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCa                                                                                                                                    >  1:4957898/1‑150 (MQ=255)
               gcCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCa                                                                                                                                    >  1:1823800/1‑150 (MQ=255)
               gcCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCa                                                                                                                                    >  1:2510846/1‑150 (MQ=255)
                   gTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGc                                                                                                                                >  2:4102445/1‑150 (MQ=255)
                      gcgGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGa                                                                                                                             <  1:3063243/150‑1 (MQ=255)
                             aGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGcc                                                                                                                      >  1:1599524/1‑150 (MQ=255)
                              gCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCg                                                                                                                     <  2:6216793/150‑1 (MQ=255)
                               cTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCgg                                                                                                                    <  1:6602236/150‑1 (MQ=255)
                               cTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCgg                                                                                                                    >  1:8841550/1‑150 (MQ=255)
                                tCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCggg                                                                                                                   >  1:8356151/1‑150 (MQ=255)
                                  acaGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGaa                                                                                                                 >  1:4428099/1‑150 (MQ=255)
                                  acaGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGaa                                                                                                                 >  1:7320625/1‑150 (MQ=255)
                                   caGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGaaa                                                                                                                >  1:1511681/1‑150 (MQ=255)
                                   caGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGaaa                                                                                                                <  1:6373551/150‑1 (MQ=255)
                                    aGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAg                                                                                                               >  2:5755970/1‑150 (MQ=255)
                                     ggCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGc                                                                                                              <  2:3356690/150‑1 (MQ=255)
                                      gCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCt                                                                                                             >  1:2192694/1‑150 (MQ=255)
                                        cACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTAc                                                                                                           >  1:3660960/1‑150 (MQ=255)
                                        cACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTAc                                                                                                           <  1:6015452/150‑1 (MQ=255)
                                         aCGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACg                                                                                                          >  1:6158287/1‑150 (MQ=255)
                                           gCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGca                                                                                                        >  1:1121117/1‑150 (MQ=255)
                                                  cAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCa                                                                                                 <  2:7301180/150‑1 (MQ=255)
                                                  cAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCa                                                                                                 >  2:1919834/1‑150 (MQ=255)
                                                  cAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCa                                                                                                 <  2:5024448/150‑1 (MQ=255)
                                                     gTCATCAGTTGCTCACCTGCCTGGCTCTTGCGGTCGTGGGCTGCGTCGCGGGTCATTGCGTTGAAGTTACCTGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTc                                                                                              <  2:8552802/150‑1 (MQ=255)
                                                     gTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTc                                                                                              <  1:3764298/150‑1 (MQ=255)
                                                       catcaGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTcac                                                                                            <  1:7567577/150‑1 (MQ=255)
                                                          caGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAAc                                                                                         <  1:2855198/150‑1 (MQ=255)
                                                           aGTTGCGAACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACg                                                                                        >  1:5825614/1‑150 (MQ=255)
                                                                cgcACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGc                                                                                   <  2:8241430/150‑1 (MQ=255)
                                                                  cACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCga                                                                                 <  2:4362417/150‑1 (MQ=255)
                                                                   aCCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCgat                                                                                >  2:2643281/1‑150 (MQ=255)
                                                                   aCCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCgat                                                                                >  1:4734334/1‑150 (MQ=255)
                                                                   aCCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCgag                                                                                >  2:5664138/1‑149 (MQ=255)
                                                                     ctgcctgGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCgatga                                                                              <  2:7212260/150‑1 (MQ=255)
                                                                     ctgcctgGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCgatga                                                                              >  1:4655068/1‑150 (MQ=255)
                                                                         ctgGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAg                                                                          <  1:4344386/150‑1 (MQ=255)
                                                                          tgGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGt                                                                         >  2:5602576/1‑150 (MQ=255)
                                                                          tgGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGt                                                                         >  2:7585956/1‑150 (MQ=255)
                                                                              tCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGct                                                                     >  2:7914868/1‑150 (MQ=255)
                                                                              tCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGct                                                                     >  1:8784514/1‑150 (MQ=255)
                                                                                      tCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCa                                                             >  1:8975899/1‑150 (MQ=255)
                                                                                      tCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCa                                                             >  1:5074859/1‑150 (MQ=255)
                                                                                      tCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCa                                                             >  2:447252/1‑150 (MQ=255)
                                                                                         tGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTg                                                          <  2:2152548/150‑1 (MQ=255)
                                                                                         tGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGAACAGTTGCTTCTTGCCAGTg                                                          <  2:3180350/150‑1 (MQ=255)
                                                                                         tGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGAACAGTTGCTTCTTGCCAGTg                                                          <  2:3955410/150‑1 (MQ=255)
                                                                                         ggggCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGGTGCTTCTTGCCAGTg                                                          <  2:4155297/149‑1 (MQ=255)
                                                                                                 gTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCAc                                                  >  1:7199985/1‑150 (MQ=255)
                                                                                                       ggTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCtt                                            <  2:847225/150‑1 (MQ=255)
                                                                                                         tCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGa                                          >  1:8143661/1‑150 (MQ=255)
                                                                                                         tCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGa                                          <  2:8898191/150‑1 (MQ=255)
                                                                                                          cATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGAc                                         <  1:8406592/150‑1 (MQ=255)
                                                                                                             ttcattGAAGGTTCCCGACGCATCTGTGACGACAAAGACATCGAAACCGGCTTCGATAGCGGACAATGCCGGGAAAGCCACGCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGaa                                      >  1:1030459/5‑150 (MQ=255)
                                                                                                             ggCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGaa                                      <  1:2050050/150‑1 (MQ=255)
                                                                                                             ggCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGaa                                      <  2:1577415/150‑1 (MQ=255)
                                                                                                             ggCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGaa                                      <  2:1511681/150‑1 (MQ=255)
                                                                                                                gTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTc                                   >  2:9143266/1‑150 (MQ=255)
                                                                                                                gTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTc                                   <  2:3477283/150‑1 (MQ=255)
                                                                                                                  tGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCtt                                 <  2:5458944/150‑1 (MQ=255)
                                                                                                                   gAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTc                                <  2:9110949/150‑1 (MQ=255)
                                                                                                                   gAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTc                                <  2:478677/150‑1 (MQ=255)
                                                                                                                   gAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTc                                <  1:3212456/150‑1 (MQ=255)
                                                                                                                     aGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGt                              >  2:3572904/1‑150 (MQ=255)
                                                                                                                      ggTTCCCGACGCATCTGTGACGACAAAGACATCGAAACCGGCTTCGATAGCGGACAATGCCGGGAAAGCCACGCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCAtt                             >  2:6918498/1‑150 (MQ=255)
                                                                                                                      ggTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGtt                             <  2:24068/150‑1 (MQ=255)
                                                                                                                        tACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGt                           <  2:2796374/150‑1 (MQ=255)
                                                                                                                          ccGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTcc                         >  2:8955348/1‑150 (MQ=255)
                                                                                                                              aGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAgg                     >  2:4946160/1‑150 (MQ=255)
                                                                                                                               ggCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGc                    <  2:407729/150‑1 (MQ=255)
                                                                                                                               cgCATCTGTGACGACAAAGACATCGAAACCGGCTTCGATAGCGGACAATGCCGGGAAAGCCACGCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGc                    <  1:6730216/149‑1 (MQ=255)
                                                                                                                                 cATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGt                  >  1:4033668/1‑150 (MQ=255)
                                                                                                                                 cATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGt                  >  1:5745484/1‑150 (MQ=255)
                                                                                                                                  aTCTGTGACGACAAAGACATCGAAACCGGCTTCGATAGCGGACAATGCCGGGAAAGCCACGCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGtt                 <  2:2903351/150‑1 (MQ=255)
                                                                                                                                  aTCTGTGACGACAAAGACATCGAAACCGGCTTCGATAGCGGACAATGCCGGGAAAGCCACGCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGtt                 <  1:5530245/150‑1 (MQ=255)
                                                                                                                                  aTCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGtt                 <  2:1138614/150‑1 (MQ=255)
                                                                                                                                  aTCAGGGACCAAGAACACGTCGAACTCTTCTTCCAGGGCCGACCGGGCCGGGACAGCTAAGCACACATCAGTCACAACGCCGGCGATGATCAGTGGCTTCTTGCCAGTGGCCTGCACTGCCTGGACGACGTCTTCGTTGTCCCAGGCGtt                 >  2:6366672/1‑150 (MQ=255)
                                                                                                                                   tCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTg                >  1:3084010/1‑150 (MQ=255)
                                                                                                                                     aGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGAt              <  1:1429672/150‑1 (MQ=255)
                                                                                                                                        gACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTg           <  1:3470463/150‑1 (MQ=255)
                                                                                                                                         aCCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTgg          <  2:3715692/150‑1 (MQ=255)
                                                                                                                                           cACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGTCCTTCACTGCCTTGACGAAGTCTTCGTTGTCACAGTCGTTGATCTGGcc        >  2:6540841/1‑150 (MQ=255)
                                                                                                                                            aCGAACACGTCGAACTCTTCTTCCAGGGCCGAAAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCa       >  2:1360099/1‑150 (MQ=255)
                                                                                                                                             cGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAg      >  1:1545451/1‑150 (MQ=255)
                                                                                                                                             cGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAg      >  1:5922846/1‑150 (MQ=255)
                                                                                                                                              gcacacGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGGTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAgg     <  2:6758949/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
CTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCG  >  NC_002947/6000577‑6000871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑