BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,000,735	T→G	6.3%	E130A (GAA→GCA) ‡	ycaC‑IV ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,000,735	0	T	G	6.3%	1077.8 / 41.7	317	E130A (GAA→GCA) ‡	ycaC‑IV	putative hydrolase
Reads supporting (aligned to +/- strand): ref base T (161/136); new base G (9/11); total (170/147)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 352 total aligned reads displayed.

CGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGG  >  NC_002947/6000586‑6000884

cGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACtctt                                                                                                                                                       <  2:5545171/150‑1 (MQ=255)

 gTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACtcttc                                                                                                                                                      >  1:6323821/1‑150 (MQ=255)

 gTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACtcttc                                                                                                                                                      >  1:2142048/1‑150 (MQ=255)

  ttGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACtcttct                                                                                                                                                     >  2:853338/1‑150 (MQ=255)

      gcCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCa                                                                                                                                                 >  1:6443136/1‑150 (MQ=255)

      gcCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCa                                                                                                                                                 <  2:415178/150‑1 (MQ=255)

       ccAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAg                                                                                                                                                >  2:372326/1‑150 (MQ=255)

             gcgGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGa                                                                                                                                          <  1:3063243/150‑1 (MQ=255)

              cgGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGAc                                                                                                                                         <  1:5997675/150‑1 (MQ=255)

               ggTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACa                                                                                                                                        <  1:5626470/150‑1 (MQ=255)

                   cAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGc                                                                                                                                    <  1:3158977/150‑1 (MQ=255)

                      cTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCgg                                                                                                                                 <  1:3524109/150‑1 (MQ=255)

                      cTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCgg                                                                                                                                 <  1:2310147/150‑1 (MQ=255)

                       tCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCggg                                                                                                                                >  1:5854523/1‑150 (MQ=255)

                        cacaGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGa                                                                                                                               <  1:4163241/150‑1 (MQ=255)

                         acaGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGaa                                                                                                                              >  1:4428099/1‑150 (MQ=255)

                         acaGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGaa                                                                                                                              >  1:7320625/1‑150 (MQ=255)

                          caGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGaaa                                                                                                                             <  1:3724804/150‑1 (MQ=255)

                          caGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGaaa                                                                                                                             <  2:5633425/150‑1 (MQ=255)

                            ggCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGc                                                                                                                           <  2:3356690/150‑1 (MQ=255)

                               cACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTAc                                                                                                                        >  1:3660960/1‑150 (MQ=255)

                                 cGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGACCTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGc                                                                                                                      >  2:1347502/1‑150 (MQ=255)

                                  gCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACGCGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGCAAGCTACGca                                                                                                                     >  1:8001025/1‑150 (MQ=255)

                                  gCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGca                                                                                                                     >  1:1121117/1‑150 (MQ=255)

                                  gCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGca                                                                                                                     >  2:3197017/1‑150 (MQ=255)

                                  gCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGca                                                                                                                     <  2:3588946/150‑1 (MQ=255)

                                         cAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCa                                                                                                              >  2:1859320/1‑150 (MQ=255)

                                         cAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCa                                                                                                              <  2:8914976/150‑1 (MQ=255)

                                             tcatcaGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTca                                                                                                          >  1:2091948/1‑150 (MQ=255)

                                              catcaGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTcac                                                                                                         <  1:7567577/150‑1 (MQ=255)

                                                 caGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAAc                                                                                                      <  1:2855198/150‑1 (MQ=255)

                                                     tGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGACAGCTACGCACACTTCAGTCACAACGCCg                                                                                                  >  1:8737650/1‑150 (MQ=255)

                                                        gcACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCg                                                                                               >  1:4737530/1‑150 (MQ=255)

                                                          aCCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCgat                                                                                             >  1:1398501/1‑150 (MQ=255)

                                                            ctgcctgGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCgatga                                                                                           >  1:4655068/1‑150 (MQ=255)

                                                              gcctgGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGa                                                                                                                                          <  2:5295776/101‑1 (MQ=255)

                                                              gcctgGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATc                                                                                         >  2:1037219/1‑150 (MQ=255)

                                                                ctgGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAg                                                                                       <  1:4344386/150‑1 (MQ=255)

                                                                     tCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGct                                                                                  >  1:6016204/1‑150 (MQ=255)

                                                                     tCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGct                                                                                  >  2:7914868/1‑150 (MQ=255)

                                                                     tCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGct                                                                                  >  2:4586243/1‑150 (MQ=255)

                                                                     tCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGct                                                                                  >  1:4983999/1‑150 (MQ=255)

                                                                        tGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGcttct                                                                               >  2:8724896/1‑150 (MQ=255)

                                                                           ggTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGc                                                                            >  2:2209353/1‑150 (MQ=255)

                                                                             tCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCa                                                                          >  1:8975899/1‑150 (MQ=255)

                                                                                tGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGAACAGTTGCTTCTTGCCAGTg                                                                       <  2:3955410/150‑1 (MQ=255)

                                                                                        gTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCAc                                                               >  2:3155426/1‑150 (MQ=255)

                                                                                         tCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACt                                                              <  1:2504142/150‑1 (MQ=255)

                                                                                                 cATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGACTTCACTGCCTTGAc                                                      <  1:145834/150‑1 (MQ=255)

                                                                                                   tGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGa                                                    >  1:1577415/1‑150 (MQ=255)

                                                                                                    ttcattGAAGGTTCCCGACGCATCTGTGACGACAAAGACATCGAAACCGGCTTCGATAGCGGACAATGCCGGGAAAGCCACGCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGaa                                                   >  1:838123/5‑150 (MQ=255)

                                                                                                    ttcattGAAGGTTCCCGACGCATCTGTGACGACAAAGACATCGAAACCGGCTTCGATAGCGGACAATGCCGGGAAAGCCACGCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGaa                                                   >  1:1030459/5‑150 (MQ=255)

                                                                                                    ggCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGaa                                                   <  1:2050050/150‑1 (MQ=255)

                                                                                                    ggCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGaa                                                   <  2:1577415/150‑1 (MQ=255)

                                                                                                    ggCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGaa                                                   <  2:1511681/150‑1 (MQ=255)

                                                                                                       gTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTc                                                <  2:3477283/150‑1 (MQ=255)

                                                                                                          gAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTc                                             >  2:4220440/1‑150 (MQ=255)

                                                                                                          gAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTc                                             <  2:478677/150‑1 (MQ=255)

                                                                                                          gAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTc                                             <  2:9110949/150‑1 (MQ=255)

                                                                                                            aGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGt                                           >  2:7541469/1‑150 (MQ=255)

                                                                                                             ggTTCCCGACGCATCTGTGACGACAAAGACATCGAAACCGGCTTCGATAGCGGACAATGCCGGGAAAGCCACGCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCAtt                                          >  2:6918498/1‑150 (MQ=255)

                                                                                                             ggTACCGGAGGCATCTGTGACCACGAACTCGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCTGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGtt                                          <  1:8809544/150‑1 (MQ=255)

                                                                                                              gTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTg                                         <  2:6328218/150‑1 (MQ=255)

                                                                                                               tACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGt                                        <  2:2796374/150‑1 (MQ=255)

                                                                                                                 ccGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTcc                                      >  2:8298013/1‑150 (MQ=255)

                                                                                                                   cgacgCATCTGTGACGACAAAGACATCGAAACCGGCTTCGATAGCGGACAATGCCGGGAAAGCCACGCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCa                                    <  1:6581095/146‑1 (MQ=255)

                                                                                                                     aGGCATCAGTGACCACGAACACGTCGAACACTTCTTCCAGGGCCGACAGGGCCGGGAAAGCAACGCACACATCAGTCACAACGCCGGCGAGGATCAGTAGCTTCTAGCCAGTGGCCAACACTGCCTAGACGAAGTCTTCGATGTCCCAgg                                  >  2:4946191/1‑150 (MQ=255)

                                                                                                                      ggCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGc                                 <  2:407993/150‑1 (MQ=255)

                                                                                                                      cgCATCTGTGACGACAAAGACATCGAAACCGGCTTCGATAGCGGACAATGCCGGGAAAGCCACGCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGc                                 <  1:6730346/149‑1 (MQ=255)

                                                                                                                         aTCTGTGACGACAAAGACATCGAAACCGGCTTCGATAGCGGACAATGCCGGGAAAGCCACGCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGtt                              <  2:2903351/150‑1 (MQ=255)

                                                                                                                         aTCTGTGACGACAAAGACATCGAAACCGGCTTCGATAGCGGACAATGCCGGGAAAGCCACGCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGtt                              <  2:4458637/150‑1 (MQ=255)

                                                                                                                          tCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTg                             >  1:3084010/1‑150 (MQ=255)

                                                                                                                          tCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTg                             <  2:813177/150‑1 (MQ=255)

                                                                                                                           cAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGa                            <  1:5783965/150‑1 (MQ=255)

                                                                                                                            aGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGAt                           <  1:1537916/150‑1 (MQ=255)

                                                                                                                              tGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCt                         <  2:2510846/150‑1 (MQ=255)

                                                                                                                                  cACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGTCCTTCACTGCCTTGACGAAGTCTTCGTTGTCACAGTCGTTGATCTGGcc                     >  2:6540841/1‑150 (MQ=255)

                                                                                                                                   aCGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCa                    >  2:9038225/1‑150 (MQ=255)

                                                                                                                                    cGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAg                   >  1:5922846/1‑150 (MQ=255)

                                                                                                                                     gcacacGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGGTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAgg                  <  2:6758949/148‑1 (MQ=255)

                                                                                                                                      aaCACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAggg                 <  1:8071427/150‑1 (MQ=255)

                                                                                                                                      aaCACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAggg                 <  1:1794437/150‑1 (MQ=255)

                                                                                                                                       ccctggtgAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAgggc                <  1:6979376/143‑1 (MQ=255)

                                                                                                                                            tCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCa           >  2:569680/1‑150 (MQ=255)

                                                                                                                                              gAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAAt         >  1:4038177/1‑150 (MQ=255)

                                                                                                                                              gAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAAt         <  2:2489128/150‑1 (MQ=255)

                                                                                                                                              gAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAAt         <  2:7108082/150‑1 (MQ=255)

                                                                                                                                               aaCTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATg        >  2:6238858/1‑150 (MQ=255)

                                                                                                                                               aaCTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATg        >  1:3767940/1‑150 (MQ=255)

                                                                                                                                                 ctctTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTa      <  1:9133913/150‑1 (MQ=255)

                                                                                                                                                  tcttctTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAg                                                  >  2:5842641/1‑105 (MQ=255)

                                                                                                                                                  tcttctTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGt                                                 <  1:5492511/106‑1 (MQ=255)

                                                                                                                                                  tcttctTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTAc     >  2:7263494/1‑150 (MQ=255)

                                                                                                                                                  tcttctTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTAc     >  2:2027030/1‑150 (MQ=255)

                                                                                                                                                  tcttctTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTAc     >  1:6190484/1‑150 (MQ=255)

                                                                                                                                                  tcttctTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTAc     >  1:5169862/1‑150 (MQ=255)

                                                                                                                                                  acttcttCCATGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTAc     <  2:9145419/149‑1 (MQ=255)

                                                                                                                                                   cttcttCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACg    <  2:2142047/150‑1 (MQ=255)

                                                                                                                                                    ttcttcCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACgg   >  1:8245136/1‑150 (MQ=255)

                                                                                                                                                     tcttcCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGGTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACggg  <  2:1182464/150‑1 (MQ=255)

CGTTGCGCCAGTCGCGGTGCAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGG  >  NC_002947/6000586‑6000884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`