BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,000,752	G→T	5.7%	A124A (GCC→GCA)	ycaC‑IV ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,000,752	0	G	T	5.7%	1175.4 / 39.4	348	A124A (GCC→GCA)	ycaC‑IV	putative hydrolase
Reads supporting (aligned to +/- strand): ref base G (179/149); new base T (9/11); total (188/160)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 357 total aligned reads displayed.

CAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTCCGGGAACAGGGC  >  NC_002947/6000605‑6000901

cAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGc                                                                                                                                                     <  1:396603/150‑1 (MQ=255)

  gCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCg                                                                                                                                                   <  2:6216793/150‑1 (MQ=255)

   cTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCgg                                                                                                                                                  <  1:2310147/150‑1 (MQ=255)

    tCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCggg                                                                                                                                                 >  1:7303552/1‑150 (MQ=255)

      acaGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGaa                                                                                                                                               >  1:4352272/1‑150 (MQ=255)

      acaGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGaa                                                                                                                                               >  2:3049198/1‑150 (MQ=255)

       caGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGaaa                                                                                                                                              <  1:2912443/150‑1 (MQ=255)

       caGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGaaa                                                                                                                                              <  2:5633425/150‑1 (MQ=255)

       caGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGaaa                                                                                                                                              >  1:1511740/1‑150 (MQ=255)

          gCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGCAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCt                                                                                                                                           <  2:6767023/150‑1 (MQ=255)

             aCGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACg                                                                                                                                        >  2:7491201/1‑150 (MQ=255)

               gCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACGCGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGCAAGCTACGca                                                                                                                                      >  1:8001025/1‑150 (MQ=255)

                    aCCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACtt                                                                                                                                 >  1:5092448/1‑150 (MQ=255)

                      cAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCa                                                                                                                               >  1:7443000/1‑150 (MQ=255)

                      cAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCa                                                                                                                               >  2:1919834/1‑150 (MQ=255)

                        ggTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGt                                                                                                                             >  1:3757191/1‑150 (MQ=255)

                         gTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTc                                                                                                                            <  1:6086831/150‑1 (MQ=255)

                         gTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTc                                                                                                                            >  1:3067933/1‑150 (MQ=255)

                         gTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTc                                                                                                                            <  2:8552705/150‑1 (MQ=255)

                         gTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTc                                                                                                                            <  2:6599228/150‑1 (MQ=255)

                          tcatcaGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTca                                                                                                                           >  1:2091948/1‑150 (MQ=255)

                              caGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAAc                                                                                                                       <  1:1076759/150‑1 (MQ=255)

                                  tGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGACAGCTACGCACACTTCAGTCACAACGCCg                                                                                                                   >  1:8737650/1‑150 (MQ=255)

                                  tGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCg                                                                                                                   <  1:4531790/150‑1 (MQ=255)

                                    cgcACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGc                                                                                                                 <  2:8241430/150‑1 (MQ=255)

                                      cACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCga                                                                                                               <  2:4362417/150‑1 (MQ=255)

                                       aCCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCgat                                                                                                              >  1:4734334/1‑150 (MQ=255)

                                       aCCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCgat                                                                                                              >  1:6004836/1‑150 (MQ=255)

                                         ctgcctgGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCgatga                                                                                                            >  1:4655068/1‑150 (MQ=255)

                                         ctgcctgGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCgatga                                                                                                            <  2:7212260/150‑1 (MQ=255)

                                          tgcctgGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCgatgat                                                                                                           <  1:3143527/150‑1 (MQ=255)

                                             ctgGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAg                                                                                                        <  1:5891228/150‑1 (MQ=255)

                                              tgGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGATCACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGt                                                                                                       <  2:6242671/150‑1 (MQ=255)

                                                  tCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGct                                                                                                   >  2:8025814/1‑150 (MQ=255)

                                                       cGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTg                                                                                              <  2:697428/150‑1 (MQ=255)

                                                        ggTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGc                                                                                             <  1:9073370/150‑1 (MQ=255)

                                                          tCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCa                                                                                           >  2:447252/1‑150 (MQ=255)

                                                             tGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTg                                                                                        <  2:1569896/150‑1 (MQ=255)

                                                             tGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGAACAGTTGCTTCTTGCCAGTg                                                                                        <  2:3955410/150‑1 (MQ=255)

                                                             tGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGAACAGTTGCTTCTTGCCAGTg                                                                                        <  2:3180350/150‑1 (MQ=255)

                                                                 cggcgTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCt                                                                                    <  2:5006652/150‑1 (MQ=255)

                                                                      tCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACt                                                                               <  1:2504142/150‑1 (MQ=255)

                                                                             tCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGa                                                                        <  2:8898191/150‑1 (MQ=255)

                                                                             tCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGa                                                                        >  1:8143661/1‑150 (MQ=255)

                                                                               aTGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACg                                                                      >  1:4235248/1‑150 (MQ=255)

                                                                                 ttcattGAAGGTTCCCGACGCATCTGTGACGACAAAGACATCGAAACCGGCTTCGATAGCGGACAATGCCGGGAAAGCCACGCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGaa                                                                    >  1:1030459/5‑150 (MQ=255)

                                                                                 ttcattGAAGGTTCCCGACGCATCTGTGACGACAAAGACATCGAAACCGGCTTCGATAGCGGACAATGCCGGGAAAGCCACGCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGaa                                                                    >  1:838123/5‑150 (MQ=255)

                                                                                 ggCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGaa                                                                    <  1:3679610/150‑1 (MQ=255)

                                                                                    gTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTc                                                                 <  2:3477271/150‑1 (MQ=255)

                                                                                    gTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTc                                                                 >  2:9143266/1‑150 (MQ=255)

                                                                                    attGAAGGTTCCCGACGCATCTGTGACGACAAAGACATCGAAACCGGCTTCGATAGCGGACAATGCCGGGAAAGCCACGCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTc                                                                 <  2:5675025/149‑1 (MQ=255)

                                                                                      tGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCtt                                                               <  2:5458944/150‑1 (MQ=255)

                                                                                       gAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTc                                                              <  1:3212456/150‑1 (MQ=255)

                                                                                        aaGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCg                                                             <  2:2645127/150‑1 (MQ=255)

                                                                                         aGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGt                                                            >  2:3572904/1‑150 (MQ=255)

                                                                                          ggTACCGGAGGCATCTGTGACCACGAACTCGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCTGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGtt                                                           <  1:8809544/150‑1 (MQ=255)

                                                                                           gTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTg                                                          >  1:3625756/1‑150 (MQ=255)

                                                                                            tACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGt                                                         <  2:2796374/150‑1 (MQ=255)

                                                                                              ccGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTcc                                                       >  2:8955348/1‑150 (MQ=255)

                                                                                               ccgacgCATCTGTGACGACAAAGACATCGAAACCGGCTTCGATAGCGGACAATGCCGGGAAAGCAACGCAGACTTCGGTCACAACACATGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTcac                                                      >  2:4744878/6‑148 (MQ=255)

                                                                                                  aGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAgg                                                   >  2:4946160/1‑150 (MQ=255)

                                                                                                   ggCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGc                                                  <  2:407993/150‑1 (MQ=255)

                                                                                                   cgCATCTGTGACGACAAAGACATCGAAACCGGCTTCGATAGCGGACAATGCCGGGAAAGCCACGCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGc                                                  <  1:6730216/149‑1 (MQ=255)

                                                                                                      aTCAGTGACCACGAACACGTCGAACTCTTGTTCGAGGGCCGAGAGGGACTTTAAAGCTACGCTCCCTTGCGTCACAACTCCGGCGATGATGAGGTGGGTCTTGCGACGTGACTTCTCTTCCTTGACGTAGTCTTCGTTGTCCCAGGCGtt                                               <  2:7644962/150‑1 (MQ=255)

                                                                                                      aTCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGtt                                               <  2:4188119/150‑1 (MQ=255)

                                                                                                      aTCAGGGACCAAGAACACGTCGAACTCTTCTTCCAGGGCCGACCGGGCCGGGACAGCTAAGCACACATCAGTCACAACGCCGGCGATGATCAGTGGCTTCTTGCCAGTGGCCTGCACTGCCTGGACGACGTCTTCGTTGTCCCAGGCGtt                                               >  2:6366672/1‑150 (MQ=255)

                                                                                                        ctgTGACCCCGAACACGTCGATCTCTTCTCCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCCGTCACAACGCCGGCGATGATCCGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGa                                             <  1:6366672/148‑1 (MQ=25)

                                                                                                         aGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGAt                                            <  1:1429672/150‑1 (MQ=255)

                                                                                                            gACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTg                                         <  2:2363610/150‑1 (MQ=255)

                                                                                                             aCCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTgg                                        <  2:3715692/150‑1 (MQ=255)

                                                                                                                 cGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAg                                    >  1:5922846/1‑150 (MQ=255)

                                                                                                                 cGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAg                                    >  1:1545451/1‑150 (MQ=255)

                                                                                                                    ccctggtgAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAgggc                                 <  1:6979376/143‑1 (MQ=255)

                                                                                                                        gTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAgggcgggc                             >  1:5824445/1‑150 (MQ=255)

                                                                                                                        gTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAgggcgggc                             >  2:1296680/1‑150 (MQ=255)

                                                                                                                         tCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGAGGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTc                                                              <  2:1134809/116‑1 (MQ=255)

                                                                                                                           gAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAAt                          >  1:6514165/1‑150 (MQ=255)

                                                                                                                           gAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAAt                          >  1:4038177/1‑150 (MQ=255)

                                                                                                                           gAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAAt                          <  2:2489731/150‑1 (MQ=255)

                                                                                                                               tcttctTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTAc                      >  1:1198801/1‑150 (MQ=255)

                                                                                                                               tcttctTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTAc                      >  1:1703807/1‑150 (MQ=255)

                                                                                                                               tcttctTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTAc                      >  1:6190484/1‑150 (MQ=255)

                                                                                                                                cttcttCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACg                     <  2:9107055/150‑1 (MQ=255)

                                                                                                                                cttcttCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACg                     <  1:5502981/150‑1 (MQ=255)

                                                                                                                                cttcttCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACg                     <  2:2142048/150‑1 (MQ=255)

                                                                                                                                 ttcttcCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACgg                    <  2:3312852/150‑1 (MQ=255)

                                                                                                                                 ttcttcCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTCCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACgg                    >  2:2033847/1‑150 (MQ=255)

                                                                                                                                    ttcCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGc                 >  2:57749/1‑150 (MQ=255)

                                                                                                                                      ccAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGt               >  1:5615050/1‑150 (MQ=255)

                                                                                                                                       cAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTc              >  2:4589065/1‑150 (MQ=255)

                                                                                                                                        aGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTcc             >  2:8790998/1‑150 (MQ=255)

                                                                                                                                        aGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTcc             >  2:1403357/1‑150 (MQ=255)

                                                                                                                                          ggCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTc                                                              <  1:4984602/99‑1 (MQ=255)

                                                                                                                                                cAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTCCGGGAACAg     <  2:167789/150‑1 (MQ=255)

                                                                                                                                                cAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTCCGGGAACAg     <  2:3147515/150‑1 (MQ=255)

                                                                                                                                                cAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTCCGGGAACAg     <  2:5854523/150‑1 (MQ=255)

                                                                                                                                                  gggCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTCCGGGAACAggg   <  1:8320398/150‑1 (MQ=255)

                                                                                                                                                   ggCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTCCGGGAACAGGGc  >  1:7681168/1‑150 (MQ=255)

                                                                                                                                                   ggCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTCCGGGAACAGGGc  <  1:2023206/150‑1 (MQ=255)

                                                                                                                                                   ggCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGGGGCCGTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTCCGGGAACAGGGc  <  1:939894/150‑1 (MQ=255)

CAGCTCACAGGCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTCCGGGAACAGGGC  >  NC_002947/6000605‑6000901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`