BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,000,764	T→C	5.5%	V120V (GTA→GTG)	ycaC‑IV ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,000,764	0	T	C	5.5%	1101.7 / 31.7	345	V120V (GTA→GTG)	ycaC‑IV	putative hydrolase
Reads supporting (aligned to +/- strand): ref base T (166/158); new base C (8/11); total (176/169)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

Only 100 of 355 total aligned reads displayed.

GCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTCCGGGAACAGGGCTTTCAGTTCTGG  >  NC_002947/6000615‑6000913

  cACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTAc                                                                                                                                                     >  1:3660960/1‑150 (MQ=255)

  cACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTAc                                                                                                                                                     <  1:6015452/150‑1 (MQ=255)

     gCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGca                                                                                                                                                  >  2:3197017/1‑150 (MQ=255)

          aCCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACtt                                                                                                                                             >  1:5092448/1‑150 (MQ=255)

            cAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCa                                                                                                                                           >  2:1919834/1‑150 (MQ=255)

            cAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCa                                                                                                                                           <  2:8914976/150‑1 (MQ=255)

              ggTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGt                                                                                                                                         <  2:5560617/150‑1 (MQ=255)

               gTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTc                                                                                                                                        <  2:8552705/150‑1 (MQ=255)

               gTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTc                                                                                                                                        >  1:3067911/1‑150 (MQ=255)

                    caGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAAc                                                                                                                                   <  1:1076759/150‑1 (MQ=255)

                        tGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGACAGCTACGCACACTTCAGTCACAACGCCg                                                                                                                               >  1:8737650/1‑150 (MQ=255)

                        tGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCg                                                                                                                               <  1:4531790/150‑1 (MQ=255)

                          cgcACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGc                                                                                                                             <  2:8241430/150‑1 (MQ=255)

                             aCCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCgat                                                                                                                          >  1:4734334/1‑150 (MQ=255)

                             aCCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCgag                                                                                                                          >  2:5664138/1‑149 (MQ=255)

                                   ctgGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAg                                                                                                                    <  1:5891228/150‑1 (MQ=255)

                                   ctgGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAg                                                                                                                    <  1:4344386/150‑1 (MQ=255)

                                    tgGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGt                                                                                                                   >  2:414485/1‑150 (MQ=255)

                                        tCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGct                                                                                                               >  2:8025814/1‑150 (MQ=255)

                                           tGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGcttct                                                                                                            >  2:8724896/1‑150 (MQ=255)

                                             cGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTg                                                                                                          >  2:4485728/1‑150 (MQ=255)

                                              ggTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGc                                                                                                         <  1:9073370/150‑1 (MQ=255)

                                                   tGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTg                                                                                                    <  2:2152548/150‑1 (MQ=255)

                                                       cggcgTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCt                                                                                                <  2:5006652/150‑1 (MQ=255)

                                                           gTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCAc                                                                                            >  2:3155426/1‑150 (MQ=255)

                                                            tCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACt                                                                                           <  1:2504142/150‑1 (MQ=255)

                                                                   tCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGa                                                                                    >  2:250244/1‑150 (MQ=255)

                                                                    cacttcattGAAGGTTCCCGACGCATCTGTGACGACAAAGACATCGAAACCGGCTTCGATAGCGGACAATGCCGGGAAAGCCACGCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGAc                                                                                   <  2:1786679/143‑1 (MQ=255)

                                                                    cATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGAc                                                                                   <  1:7567882/150‑1 (MQ=255)

                                                                    cATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGAc                                                                                   <  1:8406592/150‑1 (MQ=255)

                                                                    cATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGACTTCACTGCCTTGAc                                                                                   <  1:145834/150‑1 (MQ=255)

                                                                      tGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGa                                                                                 >  1:5004311/1‑150 (MQ=255)

                                                                       ttcattGAAGGTTCCCGACGCATCTGTGACGACAAAGACATCGAAACCGGCTTCGATAGCGGACAATGCCGGGAAAGCCACGCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGaa                                                                                >  1:1030459/5‑150 (MQ=255)

                                                                       ggCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGaa                                                                                <  2:1511740/150‑1 (MQ=255)

                                                                          gTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTc                                                                             <  2:3477283/150‑1 (MQ=255)

                                                                          attGAAGGTTCCCGACGCATCTGTGACGACAAAGACATCGAAACCGGCTTCGATAGCGGACAATGCCGGGAAAGCCACGCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTc                                                                             <  2:6315253/149‑1 (MQ=255)

                                                                             gAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTc                                                                          <  1:3212456/150‑1 (MQ=255)

                                                                             gAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTc                                                                          <  2:9110949/150‑1 (MQ=255)

                                                                             gAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTc                                                                          <  2:478677/150‑1 (MQ=255)

                                                                                ggTTCCCGACGCATCTGTGACGACAAAGACATCGAAACCGGCTTCGATAGCGGACAATGCCGGGAAAGCCACGCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCAtt                                                                       <  2:5949915/150‑1 (MQ=255)

                                                                                ggTTCCCGACGCATCTGTGACGACAAAGACATCGAAACCGGCTTCGATAGCGGACAATGCCGGGAAAGCCACGCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCAtt                                                                       >  2:6918498/1‑150 (MQ=255)

                                                                                ggTACCGGAGGCATCTGTGACCACGAACTCGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCTGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGtt                                                                       <  1:8809544/150‑1 (MQ=255)

                                                                                ggTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGtt                                                                       <  2:24068/150‑1 (MQ=255)

                                                                                 gTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTg                                                                      >  1:8237673/1‑150 (MQ=255)

                                                                                   aCCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTc                                                                    <  2:2879724/150‑1 (MQ=255)

                                                                                     cGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTccc                                                                  >  2:7095481/1‑150 (MQ=255)

                                                                                      cgacgCATCTGTGACGACAAAGACATCGAAACCGGCTTCGATAGCGGACAATGCCGGGAAAGCCACGCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCa                                                                 <  1:6581095/146‑1 (MQ=255)

                                                                                         ggCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGc                                                              <  2:407729/150‑1 (MQ=255)

                                                                                           cATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGt                                                            >  1:5745484/1‑150 (MQ=255)

                                                                                            aTCTGTGACGACAAAGACATCGAAACCGGCTTCGATAGCGGACAATGCCGGGAAAGCCACGCAGACTTCGGTCACAACACCTGCGATGAGCAACTGCTTCTTGCCGGTGGCCTTCACTGCCTTGACGAATTCCTCATTGTCCCAGGCGtt                                                           <  1:5530245/150‑1 (MQ=255)

                                                                                            aTCAGTGACCACGAACACGTCGAACTCTTGTTCGAGGGCCGAGAGGGACTTTAAAGCTACGCTCCCTTGCGTCACAACTCCGGCGATGATGAGGTGGGTCTTGCGACGTGACTTCTCTTCCTTGACGTAGTCTTCGTTGTCCCAGGCGtt                                                           <  2:7644962/150‑1 (MQ=255)

                                                                                            aTCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGtt                                                           >  1:7303127/1‑150 (MQ=255)

                                                                                            aTCAGGGACCAAGAACACGTCGAACTCTTCTTCCAGGGCCGACCGGGCCGGGACAGCTAAGCACACATCAGTCACAACGCCGGCGATGATCAGTGGCTTCTTGCCAGTGGCCTGCACTGCCTGGACGACGTCTTCGTTGTCCCAGGCGtt                                                           >  2:6366672/1‑150 (MQ=255)

                                                                                             tCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTg                                                          >  1:3084010/1‑150 (MQ=255)

                                                                                              ctgTGACCCCGAACACGTCGATCTCTTCTCCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCCGTCACAACGCCGGCGATGATCCGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGa                                                         <  1:6366672/148‑1 (MQ=25)

                                                                                                     cACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGTCCTTCACTGCCTTGACGAAGTCTTCGTTGTCACAGTCGTTGATCTGGcc                                                  >  2:6540841/1‑150 (MQ=255)

                                                                                                      aCGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCa                                                 >  2:9038225/1‑150 (MQ=255)

                                                                                                       cGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAg                                                >  1:1545451/1‑150 (MQ=255)

                                                                                                       cGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAg                                                >  1:5922893/1‑150 (MQ=255)

                                                                                                        gcacacGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGGTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAgg                                               <  2:6758949/148‑1 (MQ=255)

                                                                                                          acacGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAgggc                                             >  2:370380/1‑150 (MQ=255)

                                                                                                            acGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAgggcgg                                           >  1:8022640/1‑150 (MQ=255)

                                                                                                              gTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAgggcgggc                                         >  1:5824445/1‑150 (MQ=255)

                                                                                                                 gAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAAt                                      <  2:3104546/150‑1 (MQ=255)

                                                                                                                 gAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAAt                                      <  1:372326/150‑1 (MQ=255)

                                                                                                                  aaCTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATg                                     >  2:6238579/1‑150 (MQ=255)

                                                                                                                   aCTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGt                                    <  1:4081094/150‑1 (MQ=255)

                                                                                                                   aCTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGt                                    <  2:2069347/150‑1 (MQ=255)

                                                                                                                     tcttctTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTAc                                  >  1:7827548/1‑150 (MQ=255)

                                                                                                                     tcttctTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTAc                                  >  1:6190484/1‑150 (MQ=255)

                                                                                                                     tcttctTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTAc                                  >  2:7263494/1‑150 (MQ=255)

                                                                                                                     acttcttCCATGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTAc                                  <  2:9145419/149‑1 (MQ=255)

                                                                                                                      cttcttCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACg                                 >  1:1741333/1‑150 (MQ=255)

                                                                                                                       ttcttcCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACgg                                <  2:3775094/150‑1 (MQ=255)

                                                                                                                       ttcttcCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACgg                                <  2:3312852/150‑1 (MQ=255)

                                                                                                                        tcttcCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGGTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACggg                               <  2:1182464/150‑1 (MQ=255)

                                                                                                                          ttcCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGc                             >  1:6093658/1‑150 (MQ=255)

                                                                                                                          ttcCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGc                             >  2:57749/1‑150 (MQ=255)

                                                                                                                            ccAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGt                           >  2:5269114/1‑150 (MQ=255)

                                                                                                                             cAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTc                          >  2:4589104/1‑150 (MQ=255)

                                                                                                                              aGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTcc                         >  2:1403357/1‑150 (MQ=255)

                                                                                                                                ggCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTCCgg                       <  1:1296680/150‑1 (MQ=255)

                                                                                                                                ggCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTCCgg                       <  2:4245862/150‑1 (MQ=255)

                                                                                                                                    gACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTCCGGGAAc                   >  2:2726155/1‑150 (MQ=255)

                                                                                                                                    gACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTCCGGGAAc                   >  1:4634146/1‑150 (MQ=255)

                                                                                                                                         ggCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTCCGGGAACAGGGc              <  1:940085/150‑1 (MQ=255)

                                                                                                                                         ggCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTCCGGGAACAGGGc              <  1:2023206/150‑1 (MQ=255)

                                                                                                                                         ggCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTCCGGGAACAGGGc              <  1:398151/150‑1 (MQ=255)

                                                                                                                                         ggCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTCCGGGAACAGGGc              >  1:2182701/1‑150 (MQ=255)

                                                                                                                                          gacGGGAAAGCTACGCACACTTCAGTCAAAACGACGGCGATGAACAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGATGATCTGGCCAGGGCGGGCAATGTACGGGGCGTCAGGGAAAAGGGCt             >  1:4784267/3‑150 (MQ=21)

                                                                                                                                            cGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTCCGGGAACAGGGCttt           <  1:2089523/150‑1 (MQ=255)

                                                                                                                                             gggAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTCCGGGAACAGGGCTTTc          <  2:8841550/150‑1 (MQ=255)

                                                                                                                                              ggAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTCCGGGAACAGGGCTTTCa         <  2:4428099/150‑1 (MQ=255)

                                                                                                                                              ggAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTCCGGGAACAGGGCTTTCa         <  1:6992849/150‑1 (MQ=255)

                                                                                                                                              ggAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTCCGGGAACAGGGCTTTCa         >  1:3347864/1‑150 (MQ=255)

                                                                                                                                              ggAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGGTGCGTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCACTGTACGGGGCGTCCGGGAACAGGGCTTTCa         <  2:7443000/150‑1 (MQ=255)

                                                                                                                                                    cTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTCCGGGAACAGGGCTTTCAGTTCTg   >  1:8163328/1‑150 (MQ=255)

                                                                                                                                                     tACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTCCGGGAACAGGGCTTTCAGTTCTgg  >  1:1180896/1‑150 (MQ=255)

                                                                                                                                                     tACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTCCGGGAACAGGGCTTTCAGTTCTgg  >  1:6877475/1‑150 (MQ=255)

                                                                                                                                                     tACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTCCGGGAACAGGGCTTTCAGTTCTgg  <  2:1121117/150‑1 (MQ=255)

GCCACGCCGAACCAGGTCATCAGTTGCGCACCTGCCTGGCTCATGCGGTCGTGGGCGGCGTCGCGGGTCATGGCGTTGAAGGTACCGGAGGCATCAGTGACCACGAACACGTCGAACTCTTCTTCCAGGGCCGACAGGGCCGGGAAAGCTACGCACACTTCAGTCACAACGCCGGCGATGATCAGTTGCTTCTTGCCAGTGGCCTTCACTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGGGCGTCCGGGAACAGGGCTTTCAGTTCTGG  >  NC_002947/6000615‑6000913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`