Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 471,768 C→A 9.8% V390V (GTG→GTT)  rpoD ← RNA polymerase sigma 70 factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947471,7680CA 9.8% 844.0 / 60.4 263V390V (GTG→GTT) rpoDRNA polymerase sigma 70 factor
Reads supporting (aligned to +/- strand):  ref base C (126/111);  new base A (16/10);  total (142/121)
Fisher's exact test for biased strand distribution p-value = 5.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 266 total aligned reads displayed.

TGACGGATCCACCAGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGCGAC  >  NC_002947/471619‑471917
                                                                                                                                                     |                                                                                                                                                     
  aCGAATCCACCATGTCGCATAGGTCGAGAACTTGTAACCACGACGATATTCGAACTTATCGACCGCCTTCATCAGACCGATATTGCCTTCCTGAATAAGATCGAGGAACTGAAGGCCACGGTTGGTGTACTTCTTGGCAATCGAGATAAc                                                                                                                                                     >  1:4761276/1‑150 (MQ=255)
    ggATCCACCAGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCa                                                                                                                                                   <  2:4204864/150‑1 (MQ=255)
    gaaTCCACCATGTCGCATAGGTCGAGAACTTGTAACCACGACGATATTCGAACTTATCGACCGCCTTCATCAGACCGATATTGCCTTCCTGAATAAGATCGAGGAACTGAAGGCCACGGTTGGTGTACTTCTTGGCAATCGAGATAAcga                                                                                                                                                   <  1:1713744/148‑3 (MQ=255)
      aTCCACCAGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGa                                                                                                                                                 >  2:3945538/1‑150 (MQ=255)
       tctaccaGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGAc                                                                                                                                                <  1:4500657/147‑1 (MQ=255)
         caccaTGTCGCATAGGTCGAGAACTTGTAACCACGACGATATTCGAACTTATCGACCGCCTTCATCAGACCGATATTGCCTTCCTGAATAAGATCGAGGAACTGAAGGCCACGGTTGGTGTACTTCTTGGCAATCGAGATAACGAGACga                                                                                                                                              >  1:8397645/1‑149 (MQ=255)
         caccaGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGc                                                                                                                                              >  2:4712473/1‑150 (MQ=255)
         caccaGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGc                                                                                                                                              >  1:955224/1‑150 (MQ=255)
          accaGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCa                                                                                                                                             >  2:4675780/1‑150 (MQ=255)
          accaGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCa                                                                                                                                             >  1:6443625/1‑150 (MQ=255)
          accaGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCa                                                                                                                                             >  1:8152767/1‑150 (MQ=255)
          accaGGTGGCATAGGTCGAGAACATGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGATGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCa                                                                                                                                             >  1:3476859/1‑150 (MQ=255)
           ccaGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAg                                                                                                                                            >  2:441883/1‑150 (MQ=255)
              tgtcgCATAGGTCGAGAACTTGTAACCACGACGATATTCGAACTTATCGACCGCCTTCATCAGACCGATATTGCCTTCCTGAATAAGATCGAGGAACTGAAGGCCACGGTTGGTGTACTTCTTGGCAATCGAGATAACGAGACGAAGGtt                                                                                                                                         >  1:1804944/5‑150 (MQ=255)
               gTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTc                                                                                                                                        >  2:8165708/1‑150 (MQ=255)
               gTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTc                                                                                                                                        <  1:4136863/150‑1 (MQ=255)
                 ggCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGc                                                                                                                                      <  1:1159698/150‑1 (MQ=255)
                    aTAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTc                                                                                                                                   >  1:2207721/1‑150 (MQ=255)
                    aTAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTc                                                                                                                                   >  1:3252648/1‑150 (MQ=255)
                    aTAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTc                                                                                                                                   >  2:7186319/1‑150 (MQ=255)
                    aTAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTc                                                                                                                                   >  1:7868630/1‑150 (MQ=255)
                      aGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGa                                                                                                                                 >  2:7621020/1‑150 (MQ=255)
                      aGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGa                                                                                                                                 <  2:6436826/150‑1 (MQ=255)
                      aGGTCGAGAACAACTAGCCCCGACGGTATTAGAACTAGTCCACCGCTGTCATCAGACCGATCTTCCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTCTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGa                                                                                                                                 >  2:5256910/1‑150 (MQ=255)
                        gTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGAcc                                                                                                                               >  1:8475640/1‑150 (MQ=255)
                        gTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGAcc                                                                                                                               <  1:2585927/150‑1 (MQ=255)
                         tCGAGAACTTGTAACCACGACGATATTCGAACTTATCGACCGCCTTCATCAGACCGATATTGCCTTCCTGAATAAGATCGAGGAACTGAAGGCCACGGTTGGTGTACTTCTTGGCAATCGAGATAACGAGACGAAGGTTGGCTTccacca                                                                                                                              >  1:3419829/1‑150 (MQ=255)
                         tCGAGAACTTGTAACCACGACGATATTCGAACTTATCGACCGCCTTCATCAGACCGATATTGCCTTCCTGAATAAGATCGAGGAACTGAAGGCCACGGTTGGTGTACTTCTTGGCAATCGAGATAACGAGACGAAGGTTGGCTTccacca                                                                                                                              >  1:7409048/1‑150 (MQ=255)
                           gagaACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCAtt                                                                                                                            >  1:5375230/1‑150 (MQ=255)
                           gagaACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCAtt                                                                                                                            >  2:278847/1‑150 (MQ=255)
                           gagaACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCAtt                                                                                                                            >  2:2272810/1‑150 (MQ=255)
                           gagaACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCAtt                                                                                                                            >  2:1982009/1‑150 (MQ=255)
                           gagaACTTGTAACCACGACGATATTCGAACTTATCGACCGCCTTCATCAGACCGATATTGCCTTCCTGAATAAGATCGAGGAACTGAAGGCCACGGTTGGTGTACTTCTTGGCAATCGAGATAACGAGACGAAGGTTGGCTTCCACCAtt                                                                                                                            <  2:1727376/150‑1 (MQ=255)
                             gaACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCAtttc                                                                                                                          <  2:5704728/150‑1 (MQ=255)
                               aCTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCAtttctt                                                                                                                        <  2:7762501/150‑1 (MQ=255)
                               aCTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCAtttctt                                                                                                                        <  2:1375024/150‑1 (MQ=255)
                                cTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCAtttcttt                                                                                                                       >  1:8586511/1‑150 (MQ=255)
                                 ttGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCAtttctttc                                                                                                                      >  1:8053178/1‑150 (MQ=255)
                                  tGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATttctttct                                                                                                                     <  1:6599368/150‑1 (MQ=255)
                                   gTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTtctttctt                                                                                                                    <  2:6066435/150‑1 (MQ=255)
                                   gTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTtctttctt                                                                                                                    <  1:1751585/150‑1 (MQ=255)
                                   gTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTtctttctt                                                                                                                    <  1:1834601/150‑1 (MQ=255)
                                   gTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTtctttctt                                                                                                                    <  1:2341752/150‑1 (MQ=255)
                                   gTAACCACGACGATATTCGAACTTATCGACCGCCTTCATCAGACCGATATTGCCTTCCTGAATAAGATCGAGGAACTGAAGGCCACGGTTGGTGTACTTCTTGGCAATCGAGATAACGAGACGAAGGTTGGCTTCCACCATTtccttctt                                                                                                                    >  1:5098211/1‑150 (MQ=255)
                                      gCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGgc                                                                                                                 >  1:995187/1‑150 (MQ=255)
                                       ccGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGgcg                                                                                                                >  1:8179191/1‑150 (MQ=255)
                                           gACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCgcggc                                                                                                            <  1:7086892/150‑1 (MQ=255)
                                           gACGATATTCGAACTTATCGACCGCCTTCATCAGACCGATATTGCCTTCCTGAATAAGATCGAGGAACTGAAGGCCACGGTTGGTGTACTTCTTGGCAATCGAGATAACGAGACGAAGGTTGGCTTCCACCATTTCCTTCTTGgcgatgg                                                                                                            <  2:6989676/150‑5 (MQ=255)
                                            aCGATATTCGAACTTATCGACCGCCTTCATCAGACCGATATTGCCTTCCTGAATAAGATCGAGGAACTGAAGGCCACGGTTGGTGTACTTCTTGGCAATCGAGATAACGAGACGAAGGTTGGCTTCCACCATTTCCTTCTTGgcgatggt                                                                                                           <  1:3880965/150‑6 (MQ=255)
                                               gTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGc                                                                                                        >  2:4868612/1‑150 (MQ=255)
                                                  ttCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCtt                                                                                                     >  1:6955619/1‑150 (MQ=255)
                                                  ttCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCtt                                                                                                     >  2:2377098/1‑150 (MQ=255)
                                                   tCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTg                                                                                                    >  2:9061074/1‑150 (MQ=255)
                                                   tCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTg                                                                                                    <  2:8761422/150‑1 (MQ=255)
                                                   tCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCCCGCCGCGccaag                                                                                                    <  2:6321182/150‑4 (MQ=255)
                                                    cGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTgg                                                                                                   >  1:2181046/1‑150 (MQ=255)
                                                    cGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTgg                                                                                                   >  2:7642980/1‑150 (MQ=255)
                                                     gAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGc                                                                                                  <  2:7982625/150‑1 (MQ=255)
                                                     gAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGAGGCGGGCCTTGGc                                                                                                  >  2:8094239/1‑150 (MQ=255)
                                                             ccACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGa                                                                                          <  1:6408312/150‑1 (MQ=255)
                                                                ccGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCg                                                                                       >  2:7104384/1‑150 (MQ=255)
                                                                  gCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGAc                                                                                     <  1:1116964/150‑1 (MQ=255)
                                                                   cTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACa                                                                                    <  2:3622020/150‑1 (MQ=255)
                                                                        atcaGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGa                                                                               >  2:6928767/1‑150 (MQ=255)
                                                                            gACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGt                                                                           >  1:294223/1‑150 (MQ=255)
                                                                               cGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGa                                                                        <  2:8170612/150‑1 (MQ=255)
                                                                                     tGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCt                                                                  <  2:1701066/150‑1 (MQ=255)
                                                                                     tGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCt                                                                  <  2:3068770/150‑1 (MQ=255)
                                                                                      gCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCtt                                                                 <  2:2539685/150‑1 (MQ=255)
                                                                                      gCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCtt                                                                 <  1:9061074/150‑1 (MQ=255)
                                                                                             tGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCg                                                          <  1:6554399/150‑1 (MQ=255)
                                                                                                 tCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCaa                                                      <  1:5920997/150‑1 (MQ=255)
                                                                                                  cAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAAc                                                     <  1:5282625/150‑1 (MQ=255)
                                                                                                    ggTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACgg                                                   <  1:845196/150‑1 (MQ=255)
                                                                                                     gTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGt                                                  >  1:3359283/1‑150 (MQ=255)
                                                                                                     gTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGt                                                  >  2:5147546/1‑150 (MQ=255)
                                                                                                          ggAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGc                                             >  1:8771189/1‑150 (MQ=255)
                                                                                                            aaCTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCg                                           <  1:8818874/150‑1 (MQ=255)
                                                                                                            aaCTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCg                                           <  1:2572762/150‑1 (MQ=255)
                                                                                                               tGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGtt                                        <  2:4964969/150‑1 (MQ=255)
                                                                                                                 cAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCATTGATCTCGGCAACGGTCAGGCCGGTTTc                                      >  2:1445107/1‑150 (MQ=255)
                                                                                                                   ggCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCgg                                    <  2:2161997/150‑1 (MQ=255)
                                                                                                                    gCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGTGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGt                                   >  2:114489/1‑150 (MQ=255)
                                                                                                                    gCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGTGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGt                                   >  2:114301/1‑150 (MQ=255)
                                                                                                                    gCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGt                                   >  2:8529858/1‑150 (MQ=255)
                                                                                                                    gCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGt                                   <  2:3252648/150‑1 (MQ=255)
                                                                                                                    gCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGt                                   <  2:7868630/150‑1 (MQ=255)
                                                                                                                             ggTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTc                          <  1:5020913/150‑1 (MQ=255)
                                                                                                                                gtACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGAt                       <  2:8428601/150‑1 (MQ=255)
                                                                                                                                 tACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATc                      >  2:2819023/1‑150 (MQ=255)
                                                                                                                                  aCTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCa                     >  1:1644023/1‑150 (MQ=255)
                                                                                                                                   cttcttGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAg                    >  1:6380666/1‑150 (MQ=255)
                                                                                                                                    ttcttGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGc                   >  1:558646/1‑150 (MQ=255)
                                                                                                                                      cttGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCtt                 <  1:7937076/150‑1 (MQ=255)
                                                                                                                                        tGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCt               <  1:7621020/150‑1 (MQ=255)
                                                                                                                                         ggCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTg              <  1:6103240/150‑1 (MQ=255)
                                                                                                                                               ggAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCa        >  1:193533/1‑150 (MQ=255)
                                                                                                                                                  aaTCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGc     >  2:5983560/1‑150 (MQ=255)
                                                                                                                                                   aTCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGcg    >  2:6667738/1‑150 (MQ=255)
                                                                                                                                                   aTCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGcg    >  2:2024583/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGACGGATCCACCAGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGCGAC  >  NC_002947/471619‑471917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑