Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 471,777 C→A 6.7% L387L (CTG→CTT)  rpoD ← RNA polymerase sigma 70 factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947471,7770CA 6.7% 874.1 / 35.4 264L387L (CTG→CTT) rpoDRNA polymerase sigma 70 factor
Reads supporting (aligned to +/- strand):  ref base C (132/113);  new base A (11/7);  total (143/121)
Fisher's exact test for biased strand distribution p-value = 6.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

CACCAGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGCGACGATGGCAGC  >  NC_002947/471628‑471926
                                                                                                                                                     |                                                                                                                                                     
 accaGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCa                                                                                                                                                      >  1:6443383/1‑150 (MQ=255)
      gTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTc                                                                                                                                                 >  2:8165708/1‑150 (MQ=255)
      gTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTAGCCTTCCTGGCTCAGGTCGAGGAACTGCAGGCCCCGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTc                                                                                                                                                 >  2:5665786/1‑150 (MQ=255)
        ggCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGc                                                                                                                                               <  1:1159698/150‑1 (MQ=255)
           aTAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTc                                                                                                                                            >  1:7868630/1‑150 (MQ=255)
           aTAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTc                                                                                                                                            <  2:4960268/150‑1 (MQ=255)
           aTAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTc                                                                                                                                            >  2:8047080/1‑150 (MQ=255)
           aTAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTc                                                                                                                                            >  1:3251921/1‑150 (MQ=255)
             aGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGa                                                                                                                                          <  1:160213/150‑1 (MQ=255)
             aGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGa                                                                                                                                          >  2:7621020/1‑150 (MQ=255)
             aGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGa                                                                                                                                          <  2:6436826/150‑1 (MQ=255)
               gTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGAcc                                                                                                                                        >  2:4848455/1‑150 (MQ=255)
               gTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGAcc                                                                                                                                        <  1:2585927/150‑1 (MQ=255)
               gTCGAGAACTTGTAACCACGACGATATTCGAACTTATCGACCGCCTTCATCAGACCGATATTGCCTTCCTGAATAAGATCGAGGAACTGAAGGCCACGGTTGGTGTACTTCTTGGCAATCGAGATAACGAGACGAAGGTTGGCTTCCAcc                                                                                                                                        >  1:1820864/1‑150 (MQ=255)
                tCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCa                                                                                                                                       >  1:3875057/1‑150 (MQ=255)
                tCGAGAACTTGTAACCACGACGATATTCGAACTTATCGACCGCCTTCATCAGACCGATATTGCCTTCCTGAATAAGATCGAGGAACTGAAGGCCACGGTTGGTGTACTTCTTGGCAATCGAGATAACGAGACGAAGGTTGGCTTccacca                                                                                                                                       >  1:3418519/1‑150 (MQ=255)
                tCGAGAACTTGTAACCACGACGATATTCGAACTTATCGACCGCCTTCATCAGACCGATATTGCCTTCCTGAATAAGATCGAGGAACTGAAGGCCACGGTTGGTGTACTTCTTGGCAATCGAGATAACGAGACGAAGGTTGGCTTccacca                                                                                                                                       >  1:7409048/1‑150 (MQ=255)
                tCGAGAACTTGTAACCACGACGATATTCGAACTTATCGACCGCCTTCATCAGACCGATATTGCCTTCCTGAATAAGATCGAGGAACTGAAGGCCACGGTTGGTGTACTTCTTGGCAATCGAGATAACGAGACGAAGGTTGGCTTccacca                                                                                                                                       >  2:310915/1‑150 (MQ=255)
                 cGAGAACTTGTAACCACGACGATATTCGAACTTATCGACCGCCTTCATCAGACCGATATTGCCTTCCTGAATAAGATCGAGGAACTGAAGGCCACGGTTGGTGTACTTCTTGGCAATCGAGATAACGAGACGAAGGTTGGCTTCCACCAt                                                                                                                                      >  2:4546660/1‑150 (MQ=255)
                   agaACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCAttt                                                                                                                                    <  2:2684198/150‑1 (MQ=255)
                    gaACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCAtttc                                                                                                                                   <  1:7670364/150‑1 (MQ=255)
                    gaACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCAtttc                                                                                                                                   <  2:5704728/150‑1 (MQ=255)
                     aaCTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCAtttct                                                                                                                                  <  2:1362237/150‑1 (MQ=255)
                        ttGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCAtttctttc                                                                                                                               >  1:8053178/1‑150 (MQ=255)
                        ttGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCAtttctttc                                                                                                                               >  2:6103240/1‑150 (MQ=255)
                        ttGTAGCCGAGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCAtttctttc                                                                                                                               >  2:1118682/1‑150 (MQ=255)
                         tGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATttctttct                                                                                                                              <  2:2799972/150‑1 (MQ=255)
                         tGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATttctttct                                                                                                                              <  1:6599368/150‑1 (MQ=255)
                          gTAGCCGGGTCGGTATTCGTACTTGTCCTCCGATTTCATGAGACAGATGTTTACTTGCTGGATCTTGTCGAGGAACTGCTGTCCACGGTTGGTGTACTTCTTGGCGATGGAATTCTCCTGTCGGAGGTTCGCCTCGTCCTTTtctttctt                                                                                                                             <  1:2341746/150‑1 (MQ=255)
                          gTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTtctttctt                                                                                                                             <  1:1751430/150‑1 (MQ=255)
                          gTAACCACGACGATATTCGAACTTATCGACCGCCTTCATCAGACCGATATTGCCTTCCTGAATAAGATCGAGGAACTGAAGGCCACGGTTGGTGTACTTCTTGGCAATCGAGATAACGAGACGAAGGTTGGCTTCCACCATTtccttctt                                                                                                                             >  1:5098211/1‑150 (MQ=255)
                              ccGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGgcg                                                                                                                         >  1:8179191/1‑150 (MQ=255)
                                gcgACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGcgcg                                                                                                                       >  2:4687589/1‑150 (MQ=255)
                                 cgacgaTATTCGAACTTATCGACCGCCTTCATCAGACCGATATTGCCTTCCTGAATAAGATCGAGGAACTGAAGGCCACGGTTGGTGTACTTCTTGGCAATCGAGATAACGAGACGAAGGTTGGCTTCCACCATTTCCTTCTTGgcgatg                                                                                                                      <  1:1934982/150‑4 (MQ=255)
                                  gACGATATTCGAACTTATCGACCGCCTTCATCAGACCGATATTGCCTTCCTGAATAAGATCGAGGAACTGAAGGCCACGGTTGGTGTACTTCTTGGCAATCGAGATAACGAGACGAAGGTTGGCTTCCACCATTTCCTTCTTGgcgatgg                                                                                                                     <  2:6989676/150‑5 (MQ=255)
                                    cGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGcggcgg                                                                                                                   <  2:2226066/150‑1 (MQ=255)
                                         ttCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCtt                                                                                                              >  1:5897934/1‑150 (MQ=255)
                                         ttCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCtt                                                                                                              >  2:2377098/1‑150 (MQ=255)
                                          tCGAACTTGTCCACCGCTTTCATCAGTCCTCTGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTg                                                                                                             <  1:6717361/150‑1 (MQ=255)
                                          tCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTg                                                                                                             <  2:2397588/150‑1 (MQ=255)
                                          tCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTg                                                                                                             <  2:8761422/150‑1 (MQ=255)
                                          tCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTg                                                                                                             >  2:9061074/1‑150 (MQ=255)
                                          tCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCCCGCCGCGccaag                                                                                                             <  2:6321182/150‑4 (MQ=255)
                                           cGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTgg                                                                                                            >  1:2181046/1‑150 (MQ=255)
                                            gAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGc                                                                                                           <  2:8193463/150‑1 (MQ=255)
                                             aaCTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCt                                                                                                          <  2:1902412/150‑1 (MQ=255)
                                             aaCTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCt                                                                                                          <  2:7681949/150‑1 (MQ=255)
                                                   tCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCg                                                                                                    >  1:6017724/1‑150 (MQ=255)
                                                    ccACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGa                                                                                                   <  1:6408312/150‑1 (MQ=255)
                                                     cACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGAt                                                                                                  >  1:5297700/1‑150 (MQ=255)
                                                       ccGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCg                                                                                                >  1:3452770/1‑150 (MQ=255)
                                                         gCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGAc                                                                                              <  1:1116964/150‑1 (MQ=255)
                                                               atcaGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGa                                                                                        >  2:6928767/1‑150 (MQ=255)
                                                                   gACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGt                                                                                    <  2:1704136/150‑1 (MQ=255)
                                                                   gACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGt                                                                                    >  1:294222/1‑150 (MQ=255)
                                                                    aCCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGtt                                                                                   >  2:2887844/1‑150 (MQ=255)
                                                                      cGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGa                                                                                 >  1:2212874/1‑150 (MQ=255)
                                                                           ttGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTcc                                                                            <  1:88024/150‑1 (MQ=255)
                                                                            tGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCt                                                                           <  2:1701066/150‑1 (MQ=255)
                                                                             gCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCtt                                                                          <  1:2418550/150‑1 (MQ=255)
                                                                                ttCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGAt                                                                       <  1:8472431/150‑1 (MQ=255)
                                                                                   cTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGAtctc                                                                    <  1:3320847/150‑1 (MQ=255)
                                                                                    tGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCg                                                                   >  2:7139511/1‑150 (MQ=255)
                                                                                    aggATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCg                                                                   <  1:9033835/149‑1 (MQ=255)
                                                                                     ggATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCgg                                                                  <  1:8567822/150‑1 (MQ=255)
                                                                                        tCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCaa                                                               >  1:7938134/1‑150 (MQ=255)
                                                                                            gTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGt                                                           <  2:8152767/150‑1 (MQ=255)
                                                                                             tCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGTGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTc                                                          <  2:4292285/150‑1 (MQ=255)
                                                                                             tCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTc                                                          >  2:5042274/1‑150 (MQ=255)
                                                                                               gAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAg                                                        <  2:5184727/150‑1 (MQ=255)
                                                                                                 ggAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGc                                                      >  1:5463521/1‑150 (MQ=255)
                                                                                                 ggAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGc                                                      >  1:930071/1‑150 (MQ=255)
                                                                                                 ggAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGc                                                      >  2:7807266/1‑150 (MQ=255)
                                                                                                     cTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGt                                                  >  1:806899/1‑150 (MQ=255)
                                                                                                      tGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGtt                                                 >  1:8397743/1‑150 (MQ=255)
                                                                                                         aGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCg                                              <  2:7583336/150‑1 (MQ=255)
                                                                                                         aGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCg                                              <  1:4070373/150‑1 (MQ=255)
                                                                                                         aGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCg                                              >  1:1765945/1‑150 (MQ=255)
                                                                                                          ggCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCgg                                             <  2:2161667/150‑1 (MQ=255)
                                                                                                           gCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGTGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGt                                            >  2:114489/1‑150 (MQ=255)
                                                                                                           gCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGt                                            <  2:7868630/150‑1 (MQ=255)
                                                                                                           gCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGt                                            >  2:8529598/1‑150 (MQ=255)
                                                                                                                  ttGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAgg                                     <  1:8689243/150‑1 (MQ=255)
                                                                                                                    ggTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTc                                   <  1:5020913/150‑1 (MQ=255)
                                                                                                                      tgtACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGa                                 >  1:6447782/1‑150 (MQ=255)
                                                                                                                       gtACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGAt                                <  2:8428601/150‑1 (MQ=255)
                                                                                                                           ttcttGGCGATGGAAATCATCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGc                            >  1:964284/1‑150 (MQ=255)
                                                                                                                                 gCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGt                      >  2:3365010/1‑150 (MQ=255)
                                                                                                                                       gAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCaa                <  2:3875057/150‑1 (MQ=255)
                                                                                                                                         aaTCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGc              >  2:5983560/1‑150 (MQ=255)
                                                                                                                                          aTCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGcg             >  1:6982181/1‑150 (MQ=255)
                                                                                                                                          aTCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGcg             <  2:1399122/150‑1 (MQ=255)
                                                                                                                                          aTCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGcg             >  2:2024583/1‑150 (MQ=255)
                                                                                                                                              ccAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGcgacga         <  1:1982009/150‑1 (MQ=255)
                                                                                                                                               cAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGCGACGAt        >  1:7224022/1‑150 (MQ=255)
                                                                                                                                               cAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGCGACGAt        >  1:6369084/1‑150 (MQ=255)
                                                                                                                                                aGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCTACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGCGACGATg       >  1:2592167/1‑150 (MQ=255)
                                                                                                                                                 gACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGCGACGATgg      <  1:7574046/150‑1 (MQ=255)
                                                                                                                                                    gCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGCGACGATGGCAg   >  1:6133908/1‑150 (MQ=255)
                                                                                                                                                    gCAGGTTCGCCTCGACCATTTCTTTCGTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGCGACGATGGCAg   <  1:4712473/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CACCAGGTGGCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGCGACGATGGCAGC  >  NC_002947/471628‑471926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑