Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 471,786 C→T 5.2% E384E (GAG→GAA)  rpoD ← RNA polymerase sigma 70 factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947471,7860CT 5.2% 787.2 / 20.1 247E384E (GAG→GAA) rpoDRNA polymerase sigma 70 factor
Reads supporting (aligned to +/- strand):  ref base C (120/114);  new base T (7/6);  total (127/120)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 253 total aligned reads displayed.

GCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGCGACGATGGCAGCGTTCTTTT  >  NC_002947/471637‑471934
                                                                                                                                                     |                                                                                                                                                    
  aTAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTc                                                                                                                                                    >  1:3251921/1‑150 (MQ=255)
  aTAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTc                                                                                                                                                    >  2:8047080/1‑150 (MQ=255)
  aTAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTc                                                                                                                                                    >  1:7868630/1‑150 (MQ=255)
  aTAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTc                                                                                                                                                    >  2:7186319/1‑150 (MQ=255)
  aTAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTc                                                                                                                                                    <  2:4960268/150‑1 (MQ=255)
  aTAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTc                                                                                                                                                    >  1:2207332/1‑150 (MQ=255)
    aGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGa                                                                                                                                                  <  2:1389569/150‑1 (MQ=255)
    aGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGa                                                                                                                                                  >  2:7621020/1‑150 (MQ=255)
    aGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGa                                                                                                                                                  <  1:160213/150‑1 (MQ=255)
       tCGAGAACTTGTAACCACGACGATATTCGAACTTATCGACCGCCTTCATCAGACCGATATTGCCTTCCTGAATAAGATCGAGGAACTGAAGGCCACGGTTGGTGTACTTCTTGGCAATCGAGATAACGAGACGAAGGTTGGCTTccacca                                                                                                                                               >  1:3419829/1‑150 (MQ=255)
       tCGAGAACTTGTAACCACGACGATATTCGAACTTATCGACCGCCTTCATCAGACCGATATTGCCTTCCTGAATAAGATCGAGGAACTGAAGGCCACGGTTGGTGTACTTCTTGGCAATCGAGATAACGAGACGAAGGTTGGCTTccacca                                                                                                                                               >  2:310915/1‑150 (MQ=255)
        cGAGAACTTGTAACCACGACGATATTCGAACTTATCGACCGCCTTCATCAGACCGATATTGCCTTCCTGAATAAGATCGAGGAACTGAAGGCCACGGTTGGTGTACTTCTTGGCAATCGAGATAACGAGACGAAGGTTGGCTTCCACCAt                                                                                                                                              >  2:4546660/1‑150 (MQ=255)
         gagaACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCAtt                                                                                                                                             >  2:2272810/1‑150 (MQ=255)
         gagaACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCAtt                                                                                                                                             >  1:5375230/1‑150 (MQ=255)
         gagaACTTGTAACCACGACGATATTCGAACTTATCGACCGCCTTCATCAGACCGATATTGCCTTCCTGAATAAGATCGAGGAACTGAAGGCCACGGTTGGTGTACTTCTTGGCAATCGAGATAACGAGACGAAGGTTGGCTTCCACCAtt                                                                                                                                             <  2:1727376/150‑1 (MQ=255)
           gaACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCAtttc                                                                                                                                           <  1:7670364/150‑1 (MQ=255)
                tGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATttctttct                                                                                                                                      <  2:2799972/150‑1 (MQ=255)
                 gTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTtctttctt                                                                                                                                     <  1:2341752/150‑1 (MQ=255)
                 gTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTtctttctt                                                                                                                                     <  2:1244617/150‑1 (MQ=255)
                 gTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTtctttctt                                                                                                                                     <  1:1834601/150‑1 (MQ=255)
                  tAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTg                                                                                                                                    <  2:2198320/150‑1 (MQ=255)
                   aaccacgacgaTATTCGAACTTATCGACCGCCTTCATCAGACCGATATTGCCTTCCTGAATAAGATCGAGGAACTGAAGGCCACGGTTGGTGTACTTCTTGGCAATCGAGATAACGAGACGAAGGTTGGCTTCCACCATTTCCTTCTTgg                                                                                                                                   <  1:3919178/145‑1 (MQ=255)
                     ccGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGgcg                                                                                                                                 >  1:8179191/1‑150 (MQ=255)
                         gACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCgcggc                                                                                                                             >  1:8597092/1‑150 (MQ=255)
                         gACGATATTCGAACTTATCGACCGCCTTCATCAGACCGATATTGCCTTCCTGAATAAGATCGAGGAACTGAAGGCCACGGTTGGTGTACTTCTTGGCAATCGAGATAACGAGACGAAGGTTGGCTTCCACCATTTCCTTCTTGgcgatgg                                                                                                                             <  2:6989676/150‑5 (MQ=255)
                           cGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGcggcgg                                                                                                                           <  2:2395404/150‑1 (MQ=255)
                             gTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGc                                                                                                                         >  2:2011634/1‑150 (MQ=255)
                             gTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGc                                                                                                                         >  1:1735249/1‑150 (MQ=255)
                             gTATTCGAACTTGTCCACCGCTTTCATCAGACCGAGGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGc                                                                                                                         <  2:1144214/150‑1 (MQ=255)
                               aTTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGGCTTGCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCTCCAGACGCAGTTTCGCCTCGACAATTTCTTTCTTGTCGAGGCGGGcgt                                                                                                                       >  2:9033835/1‑148 (MQ=255)
                                 tCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTg                                                                                                                     <  2:8761422/150‑1 (MQ=255)
                                  cGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTgg                                                                                                                    <  2:1045889/150‑1 (MQ=255)
                                   gAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGc                                                                                                                   <  2:8193463/150‑1 (MQ=255)
                                   gAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGAGGCGGGCCTTGGc                                                                                                                   >  2:8094239/1‑150 (MQ=255)
                                    aaCTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCt                                                                                                                  <  2:7681949/150‑1 (MQ=255)
                                     aCTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCtt                                                                                                                 >  1:8859934/1‑150 (MQ=255)
                                      cTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTc                                                                                                                <  2:2028350/150‑1 (MQ=255)
                                           ccACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCgt                                                                                                           >  2:6029876/1‑149 (MQ=255)
                                           ccACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGa                                                                                                           <  1:6408312/150‑1 (MQ=255)
                                            cACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGAt                                                                                                          >  2:2957630/1‑150 (MQ=255)
                                             aCCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATc                                                                                                         >  1:92829/1‑150 (MQ=255)
                                             aCCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAACCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATc                                                                                                         >  2:2687867/1‑150 (MQ=255)
                                              ccGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCg                                                                                                        >  1:3452770/1‑150 (MQ=255)
                                                    tcatcaGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGc                                                                                                  >  2:6621056/1‑150 (MQ=255)
                                                      atcaGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGa                                                                                                >  2:6928767/1‑150 (MQ=255)
                                                          gACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGt                                                                                            >  1:294223/1‑150 (MQ=255)
                                                             cGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGa                                                                                         >  1:2212874/1‑150 (MQ=255)
                                                                  ttGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTcc                                                                                    <  1:88024/150‑1 (MQ=255)
                                                                       ttCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGAt                                                                               <  1:8472431/150‑1 (MQ=255)
                                                                       ttCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACAGGCGACGGTTGATTTCCTTGAt                                                                               >  2:8170014/1‑150 (MQ=255)
                                                                           tGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCg                                                                           <  1:274337/150‑1 (MQ=255)
                                                                           tGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCg                                                                           >  2:7139511/1‑150 (MQ=255)
                                                                            ggATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCgg                                                                          <  1:8567822/150‑1 (MQ=255)
                                                                               tCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCaa                                                                       >  1:7938134/1‑150 (MQ=255)
                                                                                cAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAAc                                                                      <  1:5282625/150‑1 (MQ=255)
                                                                                   gTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGt                                                                   >  1:3359283/1‑150 (MQ=255)
                                                                                   gTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGt                                                                   <  2:1701654/150‑1 (MQ=255)
                                                                                    tCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTc                                                                  >  2:5042274/1‑150 (MQ=255)
                                                                                      gAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAg                                                                <  2:5184727/150‑1 (MQ=255)
                                                                                        ggAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGc                                                              >  2:7807266/1‑150 (MQ=255)
                                                                                          aaCTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCg                                                            <  1:2572762/150‑1 (MQ=255)
                                                                                            cTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGt                                                          >  1:806899/1‑150 (MQ=255)
                                                                                             tGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGtt                                                         >  1:8397743/1‑150 (MQ=255)
                                                                                               cAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCATTGATCTCGGCAACGGTCAGGCCGGTTTc                                                       >  2:1445107/1‑150 (MQ=255)
                                                                                                aGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCg                                                      >  1:1765945/1‑150 (MQ=255)
                                                                                                aGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCg                                                      <  1:4070373/150‑1 (MQ=255)
                                                                                                aGGCCACGGTTGGTGTACTTCTTGGCAATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCg                                                      <  2:633557/150‑1 (MQ=255)
                                                                                                  gCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGt                                                    >  2:8529598/1‑150 (MQ=255)
                                                                                                  gCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGt                                                    <  2:7868630/150‑1 (MQ=255)
                                                                                                  gCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGt                                                    <  2:3252648/150‑1 (MQ=255)
                                                                                                    cACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGtct                                                  >  2:4692256/1‑150 (MQ=255)
                                                                                                    cACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGtct                                                  >  1:5095281/1‑150 (MQ=255)
                                                                                                     aCGGTTGGTGTACTTCTTGGTGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGtctc                                                 <  1:6706862/150‑1 (MQ=255)
                                                                                                           ggTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTc                                           <  1:5020913/150‑1 (MQ=255)
                                                                                                             tgtACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGa                                         >  2:6291732/1‑150 (MQ=255)
                                                                                                              gtACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGAt                                        <  2:8428601/150‑1 (MQ=255)
                                                                                                              gtACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGAt                                        <  1:5990788/150‑1 (MQ=255)
                                                                                                                  ttcttGGCGATGGAAATCATCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGc                                    >  1:964284/1‑150 (MQ=255)
                                                                                                                  ttcttGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGc                                    >  1:558646/1‑150 (MQ=255)
                                                                                                                       ggCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGTTTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTg                               >  1:7430164/1‑150 (MQ=255)
                                                                                                                       ggCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTg                               <  1:1118682/150‑1 (MQ=255)
                                                                                                                       ggCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTg                               <  1:6103240/150‑1 (MQ=255)
                                                                                                                        gCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGt                              >  1:2029730/1‑150 (MQ=255)
                                                                                                                             ggAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCa                         >  1:193533/1‑150 (MQ=255)
                                                                                                                              gAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCaa                        <  2:3875057/150‑1 (MQ=255)
                                                                                                                              gAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTGTCTGGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCaa                        <  2:5870716/150‑1 (MQ=255)
                                                                                                                                 aTCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGcg                     >  1:6982181/1‑150 (MQ=255)
                                                                                                                                    aCCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGcgacg                  >  2:5452417/1‑150 (MQ=255)
                                                                                                                                      cAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGCGACGAt                >  1:7224022/1‑150 (MQ=255)
                                                                                                                                      cAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGCGACGAt                >  1:6369084/1‑150 (MQ=255)
                                                                                                                                       aGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCTACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGCGACGATg               >  1:2591836/1‑150 (MQ=255)
                                                                                                                                       aGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGCGACGATg               <  1:6585608/150‑1 (MQ=255)
                                                                                                                                       aGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGCGACGATg               >  2:2406516/1‑150 (MQ=255)
                                                                                                                                        gACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGCGACGATgg              <  1:7574046/150‑1 (MQ=255)
                                                                                                                                          cGCAGGTTCGCCTCGACCTTTTCTTTCTTGGCGCGGCGGGCCTTGGCGTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGCGACGATGGCa            <  1:8047080/150‑1 (MQ=255)
                                                                                                                                           gCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGCGACGATGGCAg           >  2:4189469/1‑150 (MQ=255)
                                                                                                                                               gTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGCGACGATGGCAGCGtt       >  1:5779654/1‑150 (MQ=255)
                                                                                                                                                  cGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGCGACGATGGCAGCGTTCtt    <  1:7186219/150‑1 (MQ=255)
                                                                                                                                                    ccTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCg                                                      <  2:931964/98‑1 (MQ=255)
                                                                                                                                                    ccTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGCGACGATGGCAGCGTTCtttt  >  1:1004593/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCATAGGTCGAGAACTTGTAGCCGCGACGGTATTCGAACTTGTCCACCGCTTTCATCAGACCGATGTTGCCTTCCTGGATCAGGTCGAGGAACTGCAGGCCACGGTTGGTGTACTTCTTGGCGATGGAAATCACCAGACGCAGGTTCGCCTCGACCATTTCTTTCTTGGCGCGGCGGGCCTTGGCTTCACCGATCGACATGCGACGGTTGATTTCCTTGATCTCGGCAACGGTCAGGCCGGTTTCGGTCTCAAGGTCGATCAGCTTCTGTTGGCAAGCGACGATGGCAGCGTTCTTTT  >  NC_002947/471637‑471934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑