BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	532,618	G→A	5.2%	noncoding (34/76 nt)	PP_t03 →	tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	532,618	0	G	A	5.2%	983.2 / 24.4	310	noncoding (34/76 nt)	PP_t03	tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base G (138/153); new base A (10/6); total (148/162)
Fisher's exact test for biased strand distribution p-value = 3.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 312 total aligned reads displayed.

AGCCGCAAGCTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCGTTCCCT  >  NC_002947/532469‑532767

  ccGCAAGCTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGt                                                                                                                                                     <  1:4150264/150‑1 (MQ=255)

    gCAAGCTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCTCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTaa                                                                                                                                                   <  2:5981002/150‑1 (MQ=255)

     cAAGCTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAg                                                                                                                                                  >  2:755572/1‑150 (MQ=255)

      aaGCTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAgg                                                                                                                                                 >  1:6206816/1‑150 (MQ=255)

          tCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGa                                                                                                                                             <  1:5162820/150‑1 (MQ=255)

          tCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGATTGACGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGa                                                                                                                                             >  2:9131116/1‑150 (MQ=255)

           ccGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTACCAAGGGTgtg                                                                                                                                            <  1:4515924/150‑3 (MQ=255)

            cgcgGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCTCACCCTTACCAAGGGTgtgc                                                                                                                                           <  1:2191167/150‑4 (MQ=255)

                    taGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGt                                                                                                                                   <  1:3617630/150‑1 (MQ=255)

                     aGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGtt                                                                                                                                  <  2:2056682/150‑1 (MQ=255)

                       tttAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGa                                                                                                                                             >  2:3620769/1‑137 (MQ=255)

                        ttAGTGGTAGAACCTCAGGCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCTCTCCAAGTTTGTCGTATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCaa                                                                                                                               <  2:8677427/150‑1 (MQ=255)

                        ttAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCaa                                                                                                                               <  2:4474269/150‑1 (MQ=255)

                              gTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGc                                                                                                                         >  1:8730374/1‑150 (MQ=255)

                              gTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGc                                                                                                                         >  2:8253749/1‑150 (MQ=255)

                              gTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGc                                                                                                                         <  1:5534195/150‑1 (MQ=255)

                                  aaCCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCaa                                                                                                                     <  1:6952794/150‑1 (MQ=255)

                                   aCCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAag                                                                                                                    <  2:3707033/150‑1 (MQ=255)

                                          ccTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCa                                                                                                             >  2:1464354/1‑150 (MQ=255)

                                            ttCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCata                                                                                                           >  1:8366124/1‑150 (MQ=255)

                                            ttCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCata                                                                                                           >  2:5549716/1‑150 (MQ=255)

                                                aaGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATaaa                                                                                                       >  2:2589986/1‑150 (MQ=255)

                                                   cTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACaa                                                                                                    <  1:1064273/150‑1 (MQ=255)

                                                   cTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACaa                                                                                                    <  2:7817959/150‑1 (MQ=255)

                                                   cTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACaa                                                                                                    <  1:7953986/150‑1 (MQ=255)

                                                   cTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACaa                                                                                                    <  2:431535/150‑1 (MQ=255)

                                                     gatgatGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAgg                                                                                                  <  2:1844362/150‑1 (MQ=255)

                                                        gatgCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAg                                                                                               >  1:3974272/1‑150 (MQ=255)

                                                               gTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGaa                                                                                        >  2:1537699/1‑150 (MQ=255)

                                                                ttCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGaaa                                                                                       <  2:2290571/150‑1 (MQ=255)

                                                                 tCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGaaaa                                                                                      <  1:5428011/150‑1 (MQ=255)

                                                                  cGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGGTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAat                                                                                     >  2:422769/1‑150 (MQ=255)

                                                                    aTTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAatat                                                                                   >  2:5799676/1‑150 (MQ=255)

                                                                     ttCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATc                                                                                  <  2:1594640/150‑1 (MQ=255)

                                                                     ttCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATc                                                                                  >  1:1759558/1‑150 (MQ=255)

                                                                     ttCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATc                                                                                  >  2:6391275/1‑150 (MQ=255)

                                                                      tCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCt                                                                                 <  1:3790986/150‑1 (MQ=255)

                                                                        ccGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTTGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGc                                                                               <  1:5792702/150‑1 (MQ=255)

                                                                        ccGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGc                                                                               >  2:5392346/1‑150 (MQ=255)

                                                                          gCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCCTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCt                                                                             <  1:2321594/150‑1 (MQ=255)

                                                                             aCCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTg                                                                          <  2:2503356/150‑1 (MQ=255)

                                                                              cccGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGt                                                                         >  1:6369060/1‑150 (MQ=255)

                                                                              cccGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGt                                                                         <  2:8366124/150‑1 (MQ=255)

                                                                               ccGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGtt                                                                        >  1:6633396/1‑150 (MQ=255)

                                                                                 gCTCCAAGTTTGTCGGTTTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGtttt                                                                      <  1:4084091/150‑1 (MQ=255)

                                                                                  cTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGa                                                                                                                                             >  1:7636993/1‑78 (MQ=255)

                                                                                  cTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTa                                                                     >  2:390731/1‑150 (MQ=255)

                                                                                     cAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCa                                                                  <  2:2147622/150‑1 (MQ=255)

                                                                                     cAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCa                                                                  >  2:1209287/1‑150 (MQ=255)

                                                                                      aaGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATg                                                                                          >  2:2815593/1‑125 (MQ=255)

                                                                                           tGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCaa                                                            <  2:2987676/150‑1 (MQ=255)

                                                                                            gTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAAAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAg                                                           >  1:1163343/1‑150 (MQ=255)

                                                                                             tCGGATTTTTCACACAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGTTAAGGGTGAGGTCAGCTGTTCAAGTCCGCTCAAGATCTCCATATAAACAAGGCAGATATGTAAATATCTGCCTTTGTTTTATCAGTGcccga                                                          <  1:2781048/150‑5 (MQ=255)

                                                                                             tCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGa                                                          >  1:5032162/1‑150 (MQ=255)

                                                                                             tCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGa                                                          >  2:8554145/1‑150 (MQ=255)

                                                                                              cGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGAc                                                         >  2:1801345/1‑150 (MQ=255)

                                                                                                gATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTa                                                       >  1:3909542/1‑150 (MQ=255)

                                                                                                    ttGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATttc                                                   >  1:9449/1‑150 (MQ=255)

                                                                                                         caAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTg                                              <  1:8322899/150‑1 (MQ=255)

                                                                                                          aaaGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATATCTTCTGc                                             >  1:3021067/1‑150 (MQ=255)

                                                                                                           aaGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCg                                            <  1:1849054/150‑1 (MQ=255)

                                                                                                                  tCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGAtt                                     <  2:3474884/150‑1 (MQ=255)

                                                                                                                  tCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGAtt                                     >  1:2645004/1‑150 (MQ=255)

                                                                                                                   cGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTg                                    >  1:1915416/1‑150 (MQ=255)

                                                                                                                    gCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGAGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGt                                   >  2:6877587/1‑150 (MQ=255)

                                                                                                                      tctTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTAt                                 <  1:9024482/150‑1 (MQ=255)

                                                                                                                      tctTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAAAATCTGCCTTTGTGTTATCAGTGCAAGACTATTTCTTCTCCGGAGGATTGTAt                                 >  2:6601801/1‑150 (MQ=255)

                                                                                                                       ctTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATc                                <  1:525474/150‑1 (MQ=255)

                                                                                                                           tAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATg                            >  1:1946498/1‑150 (MQ=255)

                                                                                                                            aGCTCAGTTTGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCTGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGTCTATTTCTTCTGCGGAGGATTGTATCGATgg                           <  2:6128927/150‑1 (MQ=255)

                                                                                                                             gCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGc                          >  1:4638243/1‑150 (MQ=255)

                                                                                                                              cTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTCTTTCTTCTGCGGAGGATTGTATCGATGGCt                         >  2:4783068/1‑150 (MQ=255)

                                                                                                                               tCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTa                        >  1:7744096/1‑150 (MQ=255)

                                                                                                                               tCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTa                        >  2:1541072/1‑150 (MQ=255)

                                                                                                                                cAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTaa                       <  1:4590217/150‑1 (MQ=255)

                                                                                                                                  gTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATTTTCATATCTGcc                                                                              >  2:3981602/1‑93 (MQ=39)

                                                                                                                                      gTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGaaaa                 <  2:5187691/150‑1 (MQ=255)

                                                                                                                                      gTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGaaaa                 <  2:1070959/150‑1 (MQ=255)

                                                                                                                                       tAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATCTCAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAg                <  2:7938303/150‑1 (MQ=255)

                                                                                                                                         gagCACCCCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATACACACGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGtt              <  2:6919346/150‑1 (MQ=255)

                                                                                                                                         gagCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGtt              <  2:6569847/150‑1 (MQ=255)

                                                                                                                                         gacctcacCCTTACCAAGGGTGTGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCACGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGtt              >  1:8231708/6‑150 (MQ=255)

                                                                                                                                          agCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGttt             <  1:8558252/150‑1 (MQ=255)

                                                                                                                                           gCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACTAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTg            >  2:7081375/1‑150 (MQ=255)

                                                                                                                                            ctcacCCTTACCAAGGGTGTGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCttt                                                                           >  2:4938127/3‑86 (MQ=255)

                                                                                                                                            ctcacCCTTACCAAGGGTGTGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCttt                                                                           <  1:4938127/84‑1 (MQ=255)

                                                                                                                                            ctcacCCTTACCAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGa           <  2:7393224/148‑1 (MQ=255)

                                                                                                                                            ctcacCCTTACCAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGa           >  2:3356102/3‑150 (MQ=255)

                                                                                                                                            cacaCCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGa           <  1:4830216/150‑1 (MQ=255)

                                                                                                                                            cacaCCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGa           <  1:1500416/150‑1 (MQ=255)

                                                                                                                                            cacaCCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGa           <  2:8730374/150‑1 (MQ=255)

                                                                                                                                             tcacCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGAt          >  2:886698/2‑150 (MQ=255)

                                                                                                                                             acacCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGAt          <  2:8977441/150‑1 (MQ=255)

                                                                                                                                              cacCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATc         >  1:5354226/1‑150 (MQ=255)

                                                                                                                                               acCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCg        >  1:7061754/1‑150 (MQ=255)

                                                                                                                                                 ccTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCGtt      >  1:3339786/1‑150 (MQ=255)

                                                                                                                                                  cTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAATGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCGttc     <  1:7438153/150‑1 (MQ=255)

                                                                                                                                                   ttGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCGttcc    >  2:7598903/1‑150 (MQ=255)

                                                                                                                                                   ttGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCGttcc    >  2:4697371/1‑150 (MQ=255)

                                                                                                                                                     ggTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCGttccct  >  1:7291251/1‑150 (MQ=255)

AGCCGCAAGCTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCGTTCCCT  >  NC_002947/532469‑532767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`