BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	532,620	T→C	5.1%	noncoding (36/76 nt)	PP_t03 →	tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	532,620	0	T	C	5.1%	1013.2 / 25.2	311	noncoding (36/76 nt)	PP_t03	tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base T (141/154); new base C (10/6); total (151/160)
Fisher's exact test for biased strand distribution p-value = 3.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 316 total aligned reads displayed.

CCGCAAGCTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCGTTCCCTTC  >  NC_002947/532471‑532769

 cGCAAGCTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTa                                                                                                                                                       >  2:4631399/1‑150 (MQ=255)

  gCAAGCTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCTCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTaa                                                                                                                                                      <  2:5981002/150‑1 (MQ=255)

    aaGCTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAgg                                                                                                                                                    <  2:1829357/150‑1 (MQ=255)

     aGCTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAggg                                                                                                                                                   <  1:5332052/150‑1 (MQ=255)

     aGCTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAggg                                                                                                                                                   <  1:3156895/150‑1 (MQ=255)

        tCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGa                                                                                                                                                <  1:7237419/150‑1 (MQ=255)

         ccGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAg                                                                                                                                               <  2:8983000/150‑1 (MQ=255)

          cgcgGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTACCAAGGGTgtgc                                                                                                                                              <  2:7568911/150‑4 (MQ=255)

             gggTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCAGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCa                                                                                                                                           >  1:4119826/1‑150 (MQ=255)

              ggTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAg                                                                                                                                          <  1:5518237/150‑1 (MQ=255)

               gTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGatctggat                                                                                                                                         >  2:1559861/1‑143 (MQ=255)

                      ttAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCaa                                                                                                                                  <  1:3934401/150‑1 (MQ=255)

                            gTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGc                                                                                                                            >  2:8558252/1‑150 (MQ=255)

                            gTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGc                                                                                                                            >  2:8253749/1‑150 (MQ=255)

                            gTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGc                                                                                                                            <  1:965010/150‑1 (MQ=255)

                             tAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCt                                                                                                                           >  1:373249/1‑150 (MQ=255)

                             tAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCACTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCt                                                                                                                           >  1:4636512/1‑150 (MQ=255)

                                aaCCTCAGCCTTCCAAGCGGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTGTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCaa                                                                                                                        <  2:3637468/150‑1 (MQ=255)

                                   cTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAagag                                                                                                                     <  2:6595784/150‑1 (MQ=255)

                                    tCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTcc                                                                                                                              <  1:2525681/140‑1 (MQ=255)

                                    tCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTcc                                                                                                                              >  2:2525681/1‑140 (MQ=255)

                                    tCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGc                                                                                                                    >  1:5192191/1‑150 (MQ=255)

                                     cAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCt                                                                                                                   <  1:9040026/150‑1 (MQ=255)

                                          ttCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCata                                                                                                              >  2:5549716/1‑150 (MQ=255)

                                          ttCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCata                                                                                                              >  1:8366124/1‑150 (MQ=255)

                                            ccAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCAtata                                                                                                            <  1:3835734/150‑1 (MQ=255)

                                              aaGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATaaa                                                                                                          >  1:2290571/1‑150 (MQ=255)

                                                 cTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑aa                                                                                                      <  1:7953986/150‑1 (MQ=255)

                                                 cTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑aa                                                                                                      <  1:1064273/150‑1 (MQ=255)

                                                  tgatgaTGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAg                                                                                                     >  2:3756473/1‑150 (MQ=255)

                                                   cctgccgCGGGTTCGATTCCCGCTACCCGCTCCAACTTTGGCGGATTGTGCACAAAGTGTTTCGCACTTGTAGGTCAGTTGGTAGATCACAGCGTTGGTAAGGGTGAGGTCAGCGGTTGTTGTCCTCTAAAGAGCTGCTGATAAAC‑AAgg                                                                                                    <  2:8710775/144‑1 (MQ=255)

                                                     tgatgCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCa                                                                                                  <  1:8584334/150‑1 (MQ=255)

                                                         gCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGata                                                                                              <  1:3532318/150‑1 (MQ=255)

                                                              ttCGATTCCCGCTACCCGCGCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGaaa                                                                                         >  2:2013560/1‑150 (MQ=255)

                                                                  aTTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAatat                                                                                     <  1:6064951/150‑1 (MQ=255)

                                                                  aTTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAatat                                                                                     >  2:5799676/1‑150 (MQ=255)

                                                                   ttCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATc                                                                                    >  2:6391275/1‑150 (MQ=255)

                                                                   ttCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATc                                                                                    >  1:1759558/1‑150 (MQ=255)

                                                                      ccGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGc                                                                                 >  2:5392346/1‑150 (MQ=255)

                                                                       cGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGcc                                                                                <  2:2086915/150‑1 (MQ=255)

                                                                        gCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAAGGCAGATATTTTCATATCTGcc                                                                                <  1:8731187/150‑1 (MQ=255)

                                                                            cccGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGt                                                                           <  1:5376714/150‑1 (MQ=255)

                                                                            cccGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGt                                                                           >  1:6369060/1‑150 (MQ=255)

                                                                            cccGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGt                                                                           <  2:8366124/150‑1 (MQ=255)

                                                                             gcgcgCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGtt                                                                          >  2:1188273/2‑150 (MQ=255)

                                                                             ccGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGtt                                                                          >  1:6633396/1‑150 (MQ=255)

                                                                             ccGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGtt                                                                          >  1:1894197/1‑150 (MQ=255)

                                                                              cGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGttt                                                                         >  1:4393509/1‑150 (MQ=255)

                                                                                cTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGa                                                                                                                                                <  2:7636993/78‑1 (MQ=255)

                                                                                cTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGa                                                                                                                                                >  1:7636993/1‑78 (MQ=255)

                                                                                cTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTa                                                                       >  2:4386500/1‑150 (MQ=255)

                                                                                cTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTa                                                                       <  1:5630242/150‑1 (MQ=255)

                                                                                cTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTa                                                                       >  2:390731/1‑150 (MQ=255)

                                                                                cTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATAAACCA‑AAGGCAGATATTTTCATATCTGCCttgtttata                                                                       <  1:6943197/150‑8 (MQ=38)

                                                                                   cAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCa                                                                    <  1:2694971/150‑1 (MQ=255)

                                                                                   cAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCa                                                                    >  2:243300/1‑150 (MQ=255)

                                                                                    aaGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATg                                                                                            <  1:2815593/125‑1 (MQ=255)

                                                                                     aGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGt                                                                  <  2:7834053/150‑1 (MQ=255)

                                                                                          gTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAA‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAg                                                             >  1:1163343/1‑150 (MQ=255)

                                                                                           tCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGa                                                            >  2:3195721/1‑150 (MQ=255)

                                                                                           tCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGa                                                            >  1:697486/1‑150 (MQ=255)

                                                                                                  ttGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATttc                                                     <  1:1935901/150‑1 (MQ=255)

                                                                                                       caAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTg                                                <  1:8322471/150‑1 (MQ=255)

                                                                                                        aaaGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGc                                               >  2:2597134/1‑150 (MQ=255)

                                                                                                        aaaGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGc                                               >  1:7395007/1‑150 (MQ=255)

                                                                                                         aaGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCg                                              <  1:4568412/150‑1 (MQ=255)

                                                                                                                tCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑ACGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGAtt                                       <  2:6694975/150‑1 (MQ=255)

                                                                                                                  gCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGt                                     <  2:4257250/150‑1 (MQ=255)

                                                                                                                  gCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGAGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGt                                     >  2:6877587/1‑150 (MQ=255)

                                                                                                                      ttGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCg                                 <  2:8897246/150‑1 (MQ=255)

                                                                                                                      ttGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCg                                 >  2:1471379/1‑150 (MQ=255)

                                                                                                                       tGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGa                                <  2:2851540/150‑1 (MQ=255)

                                                                                                                        gTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGAt                               <  2:225025/150‑1 (MQ=255)

                                                                                                                          aGCTCAGTTTGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCTGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGTCTATTTCTTCTGCGGAGGATTGTATCGATgg                             <  2:6128927/150‑1 (MQ=255)

                                                                                                                          aGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATgg                             <  2:1324226/150‑1 (MQ=255)

                                                                                                                            cTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTCTTTCTTCTGCGGAGGATTGTATCGATGGCt                           >  2:4783068/1‑150 (MQ=255)

                                                                                                                             tCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTa                          >  1:4731322/1‑150 (MQ=255)

                                                                                                                             tCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTa                          >  2:1540385/1‑150 (MQ=255)

                                                                                                                             tCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTa                          >  1:7744096/1‑150 (MQ=255)

                                                                                                                                gTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATTTTCATATCTGcc                                                                                >  2:3981602/1‑93 (MQ=39)

                                                                                                                                gTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAgg                       >  1:5023271/1‑150 (MQ=255)

                                                                                                                                   ggTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCgga                                            <  2:4699462/126‑1 (MQ=255)

                                                                                                                                    gTAGAGCACACCCTTTGTAAGTGTGAGGTCAGCGTTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGACTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGaaaa                   <  2:1605388/150‑1 (MQ=255)

                                                                                                                                    gTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGaaaa                   <  2:5187691/150‑1 (MQ=255)

                                                                                                                                    gTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGaaaa                   <  1:4870473/150‑1 (MQ=255)

                                                                                                                                    gTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGaaaa                   >  2:8118881/1‑150 (MQ=255)

                                                                                                                                       gagCACCCCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATACAC‑ACGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGtt                <  2:6919346/150‑1 (MQ=255)

                                                                                                                                       gagCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGACAAGtt                >  1:2147736/1‑150 (MQ=255)

                                                                                                                                       gacctcacCCTTACCAAGGGTGTGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCACGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGtt                >  1:8231708/6‑150 (MQ=255)

                                                                                                                                         gCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑TAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTg              >  2:7081375/1‑150 (MQ=255)

                                                                                                                                         gCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTg              >  1:5131582/1‑150 (MQ=255)

                                                                                                                                          ctcacCCTTACCAAGGGTGTGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCttt                                                                             >  2:4938127/3‑86 (MQ=255)

                                                                                                                                          ctcacCCTTACCAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGa             <  2:7393224/148‑1 (MQ=255)

                                                                                                                                          ctcacCCTTACCAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGa             >  1:4014626/3‑150 (MQ=255)

                                                                                                                                          cacaCCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGa             <  1:1500416/150‑1 (MQ=255)

                                                                                                                                           acacCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGAt            <  2:344272/150‑1 (MQ=255)

                                                                                                                                            cacCCTTACCAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATc           >  2:8769016/1‑150 (MQ=255)

                                                                                                                                                    gTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCGttccctt   >  2:9144394/1‑150 (MQ=255)

                                                                                                                                                     tAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCGttcccttc  >  2:3517870/1‑150 (MQ=255)

                                                                                                                                                     tAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCGttcccttc  >  1:1820938/1‑150 (MQ=255)

CCGCAAGCTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAAC‑AAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCGTTCCCTTC  >  NC_002947/532471‑532769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`