Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 542,516 G→T 5.4% P251P (CCG→CCT)  rpoC → DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947542,5160GT 5.4% 854.6 / 24.2 252P251P (CCG→CCT) rpoCDNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base G (135/103);  new base T (4/10);  total (139/113)
Fisher's exact test for biased strand distribution p-value = 5.24e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 256 total aligned reads displayed.

GCGAAGAAATTCCGCAGACCAACTCGGAAACCAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGA  >  NC_002947/542368‑542665
                                                                                                                                                    |                                                                                                                                                     
gcgAAGAAATTCCGCAGACCAACTCGGAAACCAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGc                                                                                                                                                      <  1:1094414/150‑1 (MQ=255)
 cgAAGAAATTCCGCAGACCAACTCGGAAACCAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCt                                                                                                                                                     <  1:8828818/150‑1 (MQ=255)
  gaagaaATTCCGCAGACCAACTCGGAAACCAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTg                                                                                                                                                    >  1:3837491/1‑150 (MQ=255)
  gaagaaATTCCGCAGACCAACTCGGAAACCAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTg                                                                                                                                                    >  1:2376298/1‑150 (MQ=255)
   aagaaATTCCGCAGACCAACTCGGAAACCAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTgg                                                                                                                                                   >  1:4850897/1‑150 (MQ=255)
       aaTTCCGCAGACCAACTCGGAAACCAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTcc                                                                                                                                               <  1:6036496/150‑1 (MQ=255)
         ttCCGCAGACCAACTCGGAAACCAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGc                                                                                                                                             <  2:1310518/150‑1 (MQ=255)
            cGCAGACCAACTCGGAAACCATGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTgg                                                                                                                                          <  2:6300024/150‑1 (MQ=255)
                  ccAACTCGGAAACCAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGAtggtg                                                                                                                                    <  1:2574723/150‑1 (MQ=255)
                   cAACTCGGAAACCAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGAtggtgg                                                                                                                                   >  1:7602506/1‑150 (MQ=255)
                   cAACTCGGAAACCAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGAtggtgg                                                                                                                                   >  2:1526345/1‑150 (MQ=255)
                    aaCTCGGAAACCAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGc                                                                                                                                  >  2:5907865/1‑150 (MQ=255)
                     aCTCGGAAACCAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGcc                                                                                                                                 <  2:3013681/150‑1 (MQ=255)
                       tCGGAAACCAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCGTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGt                                                                                                                               >  2:4355594/1‑150 (MQ=255)
                        cGGAAACCAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGtt                                                                                                                              <  1:7697874/150‑1 (MQ=255)
                            aaCCAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTcgc                                                                                                                          <  2:3824341/150‑1 (MQ=255)
                            aaCCAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTcgc                                                                                                                          <  1:166270/150‑1 (MQ=255)
                            aaCCAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTCcgc                                                                                                                          >  1:5323894/1‑150 (MQ=255)
                               cAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGAc                                                                                                                       >  2:6835189/1‑150 (MQ=255)
                               cAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGAc                                                                                                                       >  1:1054040/1‑150 (MQ=255)
                               cAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGAc                                                                                                                       >  2:2238502/1‑150 (MQ=255)
                                  gATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTc                                                                                                                    <  1:7052472/150‑1 (MQ=255)
                                      aagaagCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGAc                                                                                                                >  2:4546234/1‑150 (MQ=255)
                                      aagaagCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGAc                                                                                                                <  2:8887764/150‑1 (MQ=255)
                                       agaagCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGAcc                                                                                                               >  2:7153805/1‑150 (MQ=255)
                                        gaagCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCt                                                                                                              <  1:1453011/150‑1 (MQ=255)
                                        gaagCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCt                                                                                                              >  1:2100589/1‑150 (MQ=255)
                                               tCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGAc                                                                                                       <  2:2361185/150‑1 (MQ=255)
                                                 cAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTg                                                                                                                                                    <  2:1833706/103‑1 (MQ=255)
                                                     cGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTAt                                                                                                 <  2:1672345/150‑1 (MQ=255)
                                                      gTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATc                                                                                                <  2:6585359/150‑1 (MQ=255)
                                                         tGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGcc                                                                                             >  1:4795249/1‑150 (MQ=255)
                                                           aaGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCgg                                                                                           >  2:7865184/1‑150 (MQ=255)
                                                             gCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGt                                                                                         <  1:64765/150‑1 (MQ=255)
                                                              cTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTg                                                                                        >  1:1186398/1‑150 (MQ=255)
                                                               tGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGa                                                                                       >  1:6259384/1‑150 (MQ=255)
                                                               tGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGa                                                                                       >  2:5113584/1‑150 (MQ=255)
                                                               tGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGa                                                                                       <  1:5339797/150‑1 (MQ=255)
                                                                   ggAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCaa                                                                                   >  2:6991609/1‑150 (MQ=255)
                                                                   ggAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCaa                                                                                   >  2:3152219/1‑150 (MQ=255)
                                                                   ggAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCaa                                                                                   >  2:7471658/1‑150 (MQ=255)
                                                                   ggAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCaa                                                                                   >  1:5132519/1‑150 (MQ=255)
                                                                    gAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAAc                                                                                  >  2:4768725/1‑150 (MQ=255)
                                                                             cAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTaacaac                                                                         <  1:5192062/150‑1 (MQ=255)
                                                                                ggCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGt                                                                      <  2:8226062/150‑1 (MQ=255)
                                                                                ggCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGt                                                                      >  1:9056128/1‑150 (MQ=255)
                                                                                 aatccggCAACCGTCCTGAGTGGATGATTATGAAGATCGTTCCGGTTATTCCACCGGATCTGCGTCCTCTGGTTCCGCTCGATGGCGGTCGTTTTGCTACATCCGATCTCAACGACCTTTACCGTCGTGTGATCAACCGTAACAACCGTc                                                                     >  1:3787076/6‑150 (MQ=255)
                                                                                  cTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCt                                                                    >  1:882700/1‑150 (MQ=255)
                                                                                  atccggCAACCGTCCTGAGTGGATGATTATGAAGATCGTTCCGGTTATTCCACCGGATCTGCGTCCTCTGGTTCCGCTCGATGGCGGTCGTTTTGCTACATCCGATCTCAACGACCTTTACCGTCGTGTGATCAACCGTAACAACCGTCt                                                                    <  1:3739776/146‑1 (MQ=255)
                                                                                   tCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTg                                                                   >  1:5880539/1‑150 (MQ=255)
                                                                                   tCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTg                                                                   >  2:5341626/1‑150 (MQ=255)
                                                                                    ccggCAACCGTCCTGAGTGGATGATTATGAAGATCGTTCCGGTTATTCCACCGGATCTGCGTCCTCTGGTTCCGCTCGATGGCGGTCGTTTTGCTACATCCGATCTCAACGACCTTTACCGTCGTGTGATCAACCGTAACAACCGTCTGa                                                                  <  2:2527779/148‑1 (MQ=255)
                                                                                    cGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGa                                                                  <  2:5880539/150‑1 (MQ=255)
                                                                                       gCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAgc                                                               <  1:6838916/150‑1 (MQ=255)
                                                                                       gCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAgc                                                               <  1:8224415/150‑1 (MQ=255)
                                                                                        cAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAgcg                                                              >  2:1394283/1‑150 (MQ=255)
                                                                                           cctgcctgAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCct                                                           >  1:3545630/1‑150 (MQ=255)
                                                                                           cctgcctgAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCct                                                           >  2:1803411/1‑150 (MQ=255)
                                                                                           cctgcctgAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCct                                                           >  2:8557185/1‑150 (MQ=255)
                                                                                            ctgcctgAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCctg                                                          >  1:3508470/1‑150 (MQ=255)
                                                                                            ctgcctgAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCctg                                                          <  2:2775499/150‑1 (MQ=255)
                                                                                              gcctgAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCctgct                                                        >  1:8142851/1‑150 (MQ=255)
                                                                                               cctgAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCctgctg                                                       <  2:8526707/150‑1 (MQ=255)
                                                                                                ctgAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGt                                                                                         >  1:2746821/1‑115 (MQ=255)
                                                                                                  gAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGAt                                                    <  2:5074807/150‑1 (MQ=255)
                                                                                                       gATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTc                                               >  2:112644/1‑150 (MQ=255)
                                                                                                       gATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTc                                               <  1:2290557/150‑1 (MQ=255)
                                                                                                       gATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACATGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTc                                               >  1:2068362/1‑150 (MQ=255)
                                                                                                            tCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGgcgc                                          >  2:692116/1‑150 (MQ=255)
                                                                                                            tCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGgcgc                                          >  1:4110451/1‑150 (MQ=255)
                                                                                                              cTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCg                                        <  2:2417943/150‑1 (MQ=255)
                                                                                                                gACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGa                                      >  2:8615305/1‑150 (MQ=255)
                                                                                                                 aCCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGAc                                     <  2:7333311/150‑1 (MQ=255)
                                                                                                                  ccGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGAca                                    <  1:8006538/150‑1 (MQ=255)
                                                                                                                       cTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACatcatc                               <  1:8579931/150‑1 (MQ=255)
                                                                                                                       cTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACatcatc                               >  2:3434447/1‑150 (MQ=255)
                                                                                                                       cTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACGTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACatcatc                               <  2:5621906/150‑1 (MQ=255)
                                                                                                                        tGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCg                              >  2:887560/1‑150 (MQ=255)
                                                                                                                         gCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGt                             >  1:7378628/1‑150 (MQ=255)
                                                                                                                         gCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGt                             >  2:1251281/1‑150 (MQ=255)
                                                                                                                           cAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTgc                           >  1:8449706/1‑150 (MQ=255)
                                                                                                                            aGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTgcg                          >  2:5270313/1‑150 (MQ=255)
                                                                                                                                 tgcCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACg                     >  2:3678017/1‑150 (MQ=255)
                                                                                                                                     gccgGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGTGCAACGaaaa                 >  1:4459899/1‑150 (MQ=255)
                                                                                                                                     gccgGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGaaaa                 <  1:3253838/150‑1 (MQ=255)
                                                                                                                                      ccgGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAg                <  2:6034622/150‑1 (MQ=255)
                                                                                                                                      ccgGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACATGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAg                >  2:5817088/1‑150 (MQ=255)
                                                                                                                                        ggACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAgcg              >  2:908860/1‑150 (MQ=255)
                                                                                                                                         gACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAgcgc             <  1:1768745/150‑1 (MQ=255)
                                                                                                                                          aCCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCa            >  2:3999408/1‑150 (MQ=255)
                                                                                                                                          aCCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCa            <  2:3837491/150‑1 (MQ=255)
                                                                                                                                           tcTGCGTCCTCTGGTTCCGCTCGATGGCGGTCGTTTTGCTACATCCGATCTCAACGACCTTTACCGTCGTGTGATCAACCGTAACAACCGTCTGAAGCGTCTTATTGAACTGCGCGCTCCTGGCATCATTATCCGTAACGAAAAGCGTAt           <  1:3030516/149‑1 (MQ=255)
                                                                                                                                           tcTGCGTCCTCTGGTTCCGCTCGATGGCGGTCGTTTTGCTACATCCGATCTCAACGACCTTTACCGTCGTGTGATCAACCGTAACAACCGTCTGAAGCGTCTTATTGAACTGCGCGCTCCTGGCATCATTATCCGTAACGAAAAGCGTAt           <  2:2963483/149‑1 (MQ=255)
                                                                                                                                           ccTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCAt           <  1:4299581/150‑1 (MQ=255)
                                                                                                                                           ccTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCAt           <  1:4355594/150‑1 (MQ=255)
                                                                                                                                              gCGTCCTCTGGTTCCGCTCGATGGCGGTCGTTTTGCTACATCCGATCTCAACGACCTTTACCGTCGTGTGATCAACCGTAACAACCGTCTGAAGCGTCTTATTGAACTGCGCGCTCCTGGCATCATTATCCGTAACGAAAAGCGTAtgtt        >  1:6785306/1‑148 (MQ=255)
                                                                                                                                              gCGTCCTCTGGTTCCGCTCGATGGCGGTCGTTTTGCTACATCCGATCTCAACGACCTTTACCGTCGTGTGATCAACCGTAACAACCGTCTGAAGCGTCTTATTGAACTGCGCGCTCCTGGCATCATTATCCGTAACGAAAAGCGTAtgtt        >  2:4191693/1‑148 (MQ=255)
                                                                                                                                               cGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCAtgctg       >  1:2512202/1‑150 (MQ=255)
                                                                                                                                                   cGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAgg   >  1:6527336/1‑150 (MQ=255)
                                                                                                                                                   cGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAgg   >  1:4023101/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCGAAGAAATTCCGCAGACCAACTCGGAAACCAAGATCAAGAAGCTTTCCAAGCGTCTGAAGCTGATGGAAGCTTTCCAGGGCTCGGGCAACCTGCCTGAGTGGATGGTCCTGACCGTCCTGCCAGTGCTGCCGCCGGACCTGCGTCCGCTGGTTCCGCTGGATGGTGGCCGTTTCGCGACTTCCGACCTGAACGACCTGTATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGA  >  NC_002947/542368‑542665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑