Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 542,717 C→T 13.2% G318G (GGC→GGT)  rpoC → DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947542,7170CT13.2% 771.5 / 87.1 294G318G (GGC→GGT) rpoCDNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base C (117/138);  new base T (25/14);  total (142/152)
Fisher's exact test for biased strand distribution p-value = 3.93e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 295 total aligned reads displayed.

TATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTC  >  NC_002947/542568‑542865
                                                                                                                                                     |                                                                                                                                                    
tATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGc                                                                                                                                                      <  2:7623234/150‑1 (MQ=255)
  tCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTc                                                                                                                                                    >  2:796218/1‑150 (MQ=255)
  tCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTc                                                                                                                                                    <  1:3152219/150‑1 (MQ=255)
   cGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCg                                                                                                                                                   <  2:6259384/150‑1 (MQ=255)
        ggTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACaa                                                                                                                                              <  2:3601583/150‑1 (MQ=255)
        ggTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACaa                                                                                                                                              <  2:4575604/150‑1 (MQ=255)
            aTCAACCGTAACAACCGTCTGAAGCGTCTTATTGAACTGCGCGCTCCTGGCATCATTATCCGTAACGAAAAGCGTATGTTGCAGGAAGCTGTTGACGCGCTGTTCGATAACGGCCGTCGTGGCCGCGTCATCACCGGTGCTAACaa                                                                                                                                              <  2:8286381/146‑1 (MQ=255)
            aTCAACCGTAACAACCGTCTGAAGCGTCTTATTGAACTGCGCGCTCCTGGCATCATTATCCGTAACGAAAAGCGTATGTTGCAGGAAGCTGTTGACGCGCTGTTCGATAACGGCCGTCGTGGCCGCGTCATCACCGGTGCTAACAAGCGt                                                                                                                                          >  1:5985891/1‑150 (MQ=255)
             tCAACCGTAACAACCGTCTGAAGCGTCTTATTGAACTGCGCGCTCCTGGCATCATTATCCGTAACGAAAAGCGTATGTTGCAGGAAGCTGTTGACGCGCTGTTCGATAACGGCCGTCGTGGCCGCGTCATCACCGGTGCTAACAAGCGTc                                                                                                                                         >  1:1384632/1‑150 (MQ=255)
             tCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTc                                                                                                                                         >  2:3844629/1‑150 (MQ=255)
               aaCCGTAACAACCGTCTGAAGCGTCTTATTGAACTGCGCGCTCCTGGCATCATTATCCGTAACGAAAAGCGTATGTTGCAGGAAGCTGTTGACGCGCTGTTCGATAACGGCCGTCGTGGCCGCGTCATCACCGGTGCTAACAAGCGTCCg                                                                                                                                       <  2:343843/150‑1 (MQ=255)
                 ccGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCt                                                                                                                                     <  1:6882790/150‑1 (MQ=255)
                 ccGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCt                                                                                                                                     <  1:103785/150‑1 (MQ=255)
                    tAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGaa                                                                                                                                  <  1:6830516/150‑1 (MQ=255)
                    tAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGaa                                                                                                                                  <  1:7327514/150‑1 (MQ=255)
                       caacCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTc                                                                                                                               >  2:2395172/1‑150 (MQ=255)
                          ccGTCTGAAGCGTCTTATTGAACTGCGCGCTCCTGGCATCATTATCCGTAACGAAAAGCGTATGTTGCAGGAAGCTGTTGACGCGCTGTTCGATAACGGCCGTCGTGGCCGCGTCATCACCGGTGCTAACAAGCGTCCGCTGAAGTCGCt                                                                                                                            <  1:8117213/150‑1 (MQ=255)
                             tCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGc                                                                                                                         >  1:9100141/1‑150 (MQ=255)
                                gAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGa                                                                                                                      >  1:6450926/1‑150 (MQ=255)
                                 aaGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGAc                                                                                                                     >  1:4210155/1‑150 (MQ=255)
                                 aaGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGAc                                                                                                                     <  1:2876499/150‑1 (MQ=255)
                                 aaGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGAc                                                                                                                     <  1:6985910/150‑1 (MQ=255)
                                   gcgcCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACAt                                                                                                                   >  2:6617862/1‑150 (MQ=255)
                                     gcCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGa                                                                                                                 >  2:8171449/1‑150 (MQ=255)
                                          ctgGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCaaa                                                                                                            <  2:5132519/150‑1 (MQ=255)
                                           tgGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAg                                                                                                           <  1:5113584/150‑1 (MQ=255)
                                           tgGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAg                                                                                                           <  1:6558402/150‑1 (MQ=255)
                                           tgGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGACCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAg                                                                                                           <  1:7153805/150‑1 (MQ=255)
                                               tCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTaa                                                                                                       <  2:642204/150‑1 (MQ=255)
                                               tCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTaa                                                                                                       <  1:6631219/150‑1 (MQ=255)
                                                    cGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAg                                                                                                  <  2:9009974/150‑1 (MQ=255)
                                                           ggACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGttt                                                                                           >  1:7465313/1‑150 (MQ=255)
                                                           ggACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGttt                                                                                           >  1:7602030/1‑150 (MQ=255)
                                                             aCATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTcc                                                                                         <  1:5817088/150‑1 (MQ=255)
                                                             aCATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTcc                                                                                         <  2:4882724/150‑1 (MQ=255)
                                                             aCATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTcc                                                                                         <  1:692226/150‑1 (MQ=255)
                                                               atcatcGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGt                                                                                       <  1:7865184/150‑1 (MQ=255)
                                                                 catcGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCa                                                                                     <  2:7920104/150‑1 (MQ=255)
                                                                 catcGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCa                                                                                     <  2:1743950/150‑1 (MQ=255)
                                                                   tcGTGCGCAACGAACAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGa                                                                                   <  1:8273709/150‑1 (MQ=255)
                                                                    cGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGaa                                                                                  <  1:112644/150‑1 (MQ=255)
                                                                     gTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAAc                                                                                 <  2:7444377/150‑1 (MQ=255)
                                                                       gcgcAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACtt                                                                               <  1:3434447/150‑1 (MQ=255)
                                                                         gcAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGGAAGCAAGGTCGTTTCCGTCAGAACTTGc                                                                             <  2:6798942/150‑1 (MQ=255)
                                                                          cAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACCTGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCt                                                                            >  2:7362854/1‑150 (MQ=255)
                                                                          cAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCt                                                                            <  2:4459899/150‑1 (MQ=255)
                                                                          cAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCt                                                                            >  1:8464831/1‑150 (MQ=255)
                                                                           aaCGAAAAGCGTATGTTGCAGGAAGCTGTTGACGCGCTGTTCGATAACGGCCGTCGTGGCCGCGTCATCACCGGTGCTAACAAGCGTCCGCTGAAGTCGCTCTCCGACATGCTCAAGGGTAAGCAGGGTCGTTTCCGTCAGAACCTtctc                                                                           <  1:8667821/150‑1 (MQ=255)
                                                                            aCGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTTAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCg                                                                          <  1:5465073/150‑1 (MQ=255)
                                                                            aCGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCg                                                                          <  1:3293387/150‑1 (MQ=255)
                                                                            aCGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCg                                                                          <  1:895144/150‑1 (MQ=255)
                                                                              gAAAAGCGTATGTTGCAGGAAGCTTTTGACGCGCTGTTCGATAACGGCCGTCGTGGCAGCGTCATCACCGGTGCTAACAAGCGTCCGCTGAAGTCGCTCTCAGACATGCTCAAGGGTAAGCAGGGTAGTTTCCGTCAGAAAATTCTCGGt                                                                        >  1:4776433/1‑150 (MQ=255)
                                                                              gAAAAGCGTATGTTGCAGGAAGCTGTTGACGCGCTGTTCGATAACGGCCGTCGTGGCCGCGTCATCACCGGTGCTAACAAGCGTCCGCTGAAGTCGCTCTCCGACATGCTCAAGGGTAAGCAGGGTCGTTTCCGTCAGAACCTTCTCGGt                                                                        >  1:2329849/1‑150 (MQ=255)
                                                                              gAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGt                                                                        <  1:2468531/150‑1 (MQ=255)
                                                                              gAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGt                                                                        >  1:4703066/1‑150 (MQ=255)
                                                                              gAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGt                                                                        >  1:1444708/1‑150 (MQ=255)
                                                                               aaaaGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTa                                                                       <  2:2512202/150‑1 (MQ=255)
                                                                                aaaGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTaa                                                                      <  1:8557185/150‑1 (MQ=255)
                                                                                 aaGCGTATGTTGCAGGAAGCTGTTGACGCGCTGTTCGATAACGGCCGTCGTGGCCGCGTCATCACCGGTGCTAACAAGCGTCCGCTGAAGTCGCTCTCCGACATGCTCAAGGGTAAGCAGGGTCGTTTCCGTCAGAACCTTCTCGGTAAg                                                                     >  2:2983137/1‑150 (MQ=255)
                                                                                  aGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGc                                                                    <  2:2667628/150‑1 (MQ=255)
                                                                                     gcATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCgtg                                                                 >  2:2154837/1‑150 (MQ=255)
                                                                                       aTGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGtt                                                               <  2:8936232/150‑1 (MQ=255)
                                                                                       aTGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGtt                                                               <  2:4023101/150‑1 (MQ=255)
                                                                                         gctgcAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGa                                                             <  2:8468708/150‑1 (MQ=255)
                                                                                         gctgcAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGa                                                             <  1:5341626/150‑1 (MQ=255)
                                                                                           tgcAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACCAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGact                                                           >  2:6031761/1‑150 (MQ=255)
                                                                                            gcAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGacta                                                          <  1:96729/150‑1 (MQ=255)
                                                                                            gcAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGacta                                                          >  1:5615424/1‑150 (MQ=255)
                                                                                               ggAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTc                                                       >  2:7739151/1‑150 (MQ=255)
                                                                                               ggAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTc                                                       <  1:8347613/150‑1 (MQ=255)
                                                                                                gAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTcc                                                      <  1:3740221/150‑1 (MQ=255)
                                                                                                gAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTcc                                                      <  1:9021802/150‑1 (MQ=255)
                                                                                                gAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTcc                                                      <  1:1819221/150‑1 (MQ=255)
                                                                                                 aaGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCg                                                     <  1:7388143/150‑1 (MQ=255)
                                                                                                  aGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCgg                                                    <  2:7001130/150‑1 (MQ=255)
                                                                                                     tgTTGACGCGCTGTTCGATAACGGCCGTCGTGGCCGCGTCATCACCGGTGCTAACAAGCGTCCGCTGAAGTCGCTCTCCGACATGCTCAAGGGTAAGCAGGGTCGTTTCCGTCAGAACCTTCTCGGTAAGCGCGTCGACTATTCGGGTCg                                                 >  1:5725960/2‑150 (MQ=255)
                                                                                                      gTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGt                                                >  1:4915693/1‑150 (MQ=255)
                                                                                                      gTTGACGCGCTGTTCGATAACGGCCGTCGTGGCCGCGTCATCACCGGTGCTAACAAGCGTCCGCTGAAGTCGCTCTCCGACATGCTCAAGGGTAAGCAGGGTCGTTTCCGTCAGAACCTTCTCGGTAAGCGCGTCGACTATTCGGGTCGt                                                >  2:2842571/1‑150 (MQ=255)
                                                                                                       ttGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGtt                                               <  2:6527336/150‑1 (MQ=255)
                                                                                                       ttGACGCGCTGTTCGATAACGGCCGTCGTGGCCGCGTCATCACCGGTGCTAACAAGCGTCCGCTGAAGTCGCTCTCCGACATGCTCAAGGGTAAGCAggg                                                                                                 <  2:7229956/100‑1 (MQ=255)
                                                                                                          aCGCGCTGTTCGATAACGGCCGTCGTGGCCGCGTCATCACCGGTGCTAACAAGCGTCCGCTGAAGTCGCTCTCCGACATGCTCAAGGGTAAGCAGGGTCGTTTCCGTCAGAACCTTCTCGGTAAGCGCGTCGACTATTCGGGTCGTTCgg                                            >  2:5938330/1‑150 (MQ=255)
                                                                                                              tctGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAAt                                        >  1:5183461/1‑150 (MQ=255)
                                                                                                                  ctgGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGac                                                            <  2:8731225/126‑1 (MQ=255)
                                                                                                                  ctgGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGac                                                            >  1:8731225/1‑126 (MQ=255)
                                                                                                                  ctgGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTAcc                                    >  2:1826184/1‑150 (MQ=255)
                                                                                                                     gACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTa                                 <  2:2227801/150‑1 (MQ=255)
                                                                                                                           ggCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCg                           >  2:1427713/1‑150 (MQ=255)
                                                                                                                                  gtGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGc                    <  2:5000441/150‑1 (MQ=255)
                                                                                                                                  gtGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGc                    <  2:2044727/150‑1 (MQ=255)
                                                                                                                                  gtGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGc                    <  1:2786884/150‑1 (MQ=255)
                                                                                                                                    ggTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGt                  <  2:9078618/150‑1 (MQ=255)
                                                                                                                                       cgcgCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTg               >  1:6219073/1‑150 (MQ=255)
                                                                                                                                         gcgaaTCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCACAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTAGGTAATTACCGTAGGTCCGACCCTGAGTCTGCa             >  2:5677552/5‑150 (MQ=255)
                                                                                                                                         gcgaaTCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCa             >  2:3763706/5‑150 (MQ=255)
                                                                                                                                            cATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCa          <  1:5062510/150‑1 (MQ=255)
                                                                                                                                            cATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCa          <  1:7043933/150‑1 (MQ=255)
                                                                                                                                                 cTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCg     <  1:5125305/150‑1 (MQ=255)
                                                                                                                                                    gCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTc  >  1:3867735/1‑150 (MQ=255)
                                                                                                                                                    gCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTc  >  1:2384969/1‑150 (MQ=255)
                                                                                                                                                    gCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTc  >  1:8019194/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TATCGCCGGGTGATCAACCGTAACAACCGTCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTC  >  NC_002947/542568‑542865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑