Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 542,744 G→C 8.4% L327L (CTG→CTC)  rpoC → DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947542,7440GC 8.4% 940.6 / 55.7 287L327L (CTG→CTC) rpoCDNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base G (120/143);  new base C (17/7);  total (137/150)
Fisher's exact test for biased strand distribution p-value = 1.97e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 287 total aligned reads displayed.

TCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCGAAGAAGATGGCCCTCGAGCTGT  >  NC_002947/542597‑542892
                                                                                                                                                   |                                                                                                                                                    
    aaGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGAc                                                                                                                                                <  1:6985910/150‑1 (MQ=255)
    aaGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGAc                                                                                                                                                <  1:2876499/150‑1 (MQ=255)
    aaGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGAc                                                                                                                                                >  1:4210155/1‑150 (MQ=255)
        gcCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGa                                                                                                                                            >  2:9277/1‑150 (MQ=255)
        gcCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGa                                                                                                                                            >  2:8171127/1‑150 (MQ=255)
        gcCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGa                                                                                                                                            <  2:5323894/150‑1 (MQ=255)
        gcCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGa                                                                                                                                            >  2:5125305/1‑150 (MQ=255)
          ctgctgGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATc                                                                                                                                          <  2:7922128/150‑1 (MQ=255)
              tgGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAg                                                                                                                                      <  1:8935444/150‑1 (MQ=255)
              tgGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGACCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAg                                                                                                                                      <  1:7153805/150‑1 (MQ=255)
                gATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGt                                                                                                                                    >  1:1291067/1‑150 (MQ=255)
                  tCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTaa                                                                                                                                  <  1:6398105/150‑1 (MQ=255)
                  tCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTaa                                                                                                                                  <  2:642204/150‑1 (MQ=255)
                   cTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAg                                                                                                                                 >  2:3805594/1‑150 (MQ=255)
                           gcCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCg                                                                                                                         >  1:2044727/1‑150 (MQ=255)
                              ggACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGttt                                                                                                                      >  1:7602030/1‑150 (MQ=255)
                                aCATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTcc                                                                                                                    <  2:4918251/150‑1 (MQ=255)
                                aCATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTcc                                                                                                                    <  1:7984258/150‑1 (MQ=255)
                                aCATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTcc                                                                                                                    <  1:5101482/150‑1 (MQ=255)
                                aCATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTcc                                                                                                                    <  1:5817088/150‑1 (MQ=255)
                                    catcGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCa                                                                                                                >  1:2621674/1‑150 (MQ=255)
                                    catcGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCa                                                                                                                <  2:1743950/150‑1 (MQ=255)
                                      tcGTGCGCAACGAACAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGa                                                                                                              <  1:8273709/150‑1 (MQ=255)
                                      tcGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGa                                                                                                              >  2:2845008/1‑150 (MQ=255)
                                      tcGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGa                                                                                                              >  1:6883702/1‑150 (MQ=255)
                                        gTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAAc                                                                                                            <  2:7444377/150‑1 (MQ=255)
                                          gcgcAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACtt                                                                                                          <  1:3434447/150‑1 (MQ=255)
                                            gcAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACCTGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGc                                                                                                        >  1:7396952/1‑150 (MQ=255)
                                            gcAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGc                                                                                                        <  1:4885186/150‑1 (MQ=255)
                                            gcAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGGAAGCAAGGTCGTTTCCGTCAGAACTTGc                                                                                                        <  2:6798942/150‑1 (MQ=255)
                                             cAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACCTGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCt                                                                                                       >  2:7362854/1‑150 (MQ=255)
                                             cAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGAAAGCAAGTTCGTTTCCGTCAGAACTTGCt                                                                                                       >  1:6765838/1‑150 (MQ=255)
                                               aCGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCg                                                                                                     <  1:895144/150‑1 (MQ=255)
                                               aCGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCg                                                                                                     <  1:3293387/150‑1 (MQ=255)
                                                 gAAAAGCGTATGTTGCAGGAAGCTTTTGACGCGCTGTTCGATAACGGCCGTCGTGGCAGCGTCATCACCGGTGCTAACAAGCGTCCGCTGAAGTCGCTCTCAGACATGCTCAAGGGTAAGCAGGGTAGTTTCCGTCAGAAAATTCTCGGt                                                                                                   >  1:4776433/1‑150 (MQ=255)
                                                 gAAAAGCGTATGTTGCAGGAAGCTGTTGACGCGCTGTTCGATAACGGCCGTCGTGGCCGCGTCATCACCGGTGCTAACAAGCGTCCGCTGAAGTCGCTCTCCGACATGCTCAAGGGTAAGCAGGGTCGTTTCCGTCAGAACCTTCTCGGt                                                                                                   >  1:2329894/1‑150 (MQ=255)
                                                 gAAAAGCGTATGTTGCAGGAAGCTGTTGACGCGCTGTTCGATAACGGACGTCGTGGCCGCGTCATCACCGGTGCTAACAAGCGTCCGCTGAAGTCGCTCTCCGACATGCTCAAGGGTAAGCAGGGTCGTTTCCGTCAGAACCTTCTCGGt                                                                                                   >  1:7826563/1‑150 (MQ=255)
                                                 gAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGt                                                                                                   >  1:4703066/1‑150 (MQ=255)
                                                 gAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGt                                                                                                   >  1:1444708/1‑150 (MQ=255)
                                                 gAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGt                                                                                                   <  1:2468531/150‑1 (MQ=255)
                                                 gAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGt                                                                                                   >  2:7701217/1‑150 (MQ=255)
                                                  aaaaGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTa                                                                                                  <  2:2512202/150‑1 (MQ=255)
                                                    aaGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAg                                                                                                <  1:8615305/150‑1 (MQ=255)
                                                     aGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGc                                                                                               <  2:2667628/150‑1 (MQ=255)
                                                       cgcATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCgt                                                                                             <  1:3678017/150‑1 (MQ=255)
                                                        gcATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCgtg                                                                                            >  2:2154837/1‑150 (MQ=255)
                                                         cATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCgtgt                                                                                           <  1:6549599/150‑1 (MQ=255)
                                                          aTGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGtt                                                                                          <  1:908860/150‑1 (MQ=255)
                                                          aTGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGtt                                                                                          <  2:8936232/150‑1 (MQ=255)
                                                          aTGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGtt                                                                                          <  2:4023101/150‑1 (MQ=255)
                                                               gcAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGacta                                                                                     <  1:96729/150‑1 (MQ=255)
                                                                  ggAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTc                                                                                  <  1:8347855/150‑1 (MQ=255)
                                                                     aGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCgg                                                                               <  2:7001130/150‑1 (MQ=255)
                                                                      gctgTTGACGCGCTGTTCGATAACGGCCGTCGTGGCCGCGTCATCAACGGTGCTAACAAGCGTCCGCTGAAGTCGCTCTCCGACATGCTCAAGGGTAAGCAGGGTCGTTTCCGTCAGAACCTTCTCGGTAAGCGCGTCGACTATTCgggt                                                                              <  1:8481710/147‑2 (MQ=255)
                                                                      gCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGc                                                                              <  1:7114215/150‑1 (MQ=255)
                                                                        tgTTGACGCGCTGTTCGATAACGGCCGTCGTGGCCGCGTCATCACCGGTGCTAACAAGCGTCCGCTGAAGTCGCTCTCCGACATGCTCAAGGGTAAGCAGGGTCGTTTCCGTCAGAACCTTCTCGGTAAGCGCGTCGACTATTCGGGTCg                                                                            >  1:5725960/2‑150 (MQ=255)
                                                                          ttGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGtt                                                                          <  2:6137713/150‑1 (MQ=255)
                                                                          ttGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGtt                                                                          <  2:1916737/150‑1 (MQ=255)
                                                                          ttGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGtt                                                                          <  2:6527336/150‑1 (MQ=255)
                                                                            gACGCTCGGCTGGACAACGGCCGTCGGGGTCGCGCCATCACGGGCTCGAACAAGCGTCCGCTGAAGTCCCGGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACGCCTCCGGCCGTTCg                                                                        <  2:2378843/150‑1 (MQ=255)
                                                                             aCGCGCTGTTCGATAACGGCCGTCGTGGCCGCGTCATCACCGGTGCTAACAAGCGTCCGCTGAAGTCGCTCTCCGACATGCTCAAGGGTAAGCAGGGTCGTTTCCGTCAGAACCTTCTCGGTAAGCGCGTCGACTATTCGGGTCGTTCgg                                                                       >  2:5938330/1‑150 (MQ=255)
                                                                             aCGCGCTGTTCGATAACGGCCGTCGTGGCCGCGTCATCACCGGTGCTAACAAGCGTCCGCTGAAGTCGCTCTCCGACATGCTCAAGGGTAAGCAGGGTCGTTTCCGTCAGAACCTTCTCGGTAAGCGCGTCGACTATTCGGGTCGTTCgg                                                                       <  1:7373755/150‑1 (MQ=255)
                                                                                   tgctgGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTa                                                                 >  1:692798/1‑150 (MQ=255)
                                                                                     ctgGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTAcc                                                               >  1:5436415/1‑150 (MQ=255)
                                                                                              ggCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCg                                                      >  2:1427713/1‑150 (MQ=255)
                                                                                                 cgtcgtGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGAcc                                                   >  1:1112489/1‑150 (MQ=255)
                                                                                                     gtGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGc                                               <  2:2044727/150‑1 (MQ=255)
                                                                                                     gtGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGc                                               <  2:5000441/150‑1 (MQ=255)
                                                                                                       ggTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGt                                             >  1:5972936/1‑150 (MQ=255)
                                                                                                        gTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCATCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTc                                            >  2:3007113/1‑150 (MQ=255)
                                                                                                          cgcgCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTg                                          >  1:6219073/1‑150 (MQ=255)
                                                                                                           gcgcCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGc                                         >  2:7855311/1‑150 (MQ=255)
                                                                                                            gcgaaTCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCACAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTAGGTAATTACCGTAGGTCCGACCCTGAGTCTGCa                                        >  2:5677552/5‑150 (MQ=255)
                                                                                                            gcgaaTCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCa                                        >  2:3763706/5‑150 (MQ=255)
                                                                                                             gcCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCAc                                       <  1:8699711/150‑1 (MQ=255)
                                                                                                               cATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCa                                     >  2:9043911/1‑150 (MQ=255)
                                                                                                                    cTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCg                                <  2:6632696/150‑1 (MQ=255)
                                                                                                                    cTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCg                                <  1:3844629/150‑1 (MQ=255)
                                                                                                                    cTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCg                                <  1:5125305/150‑1 (MQ=255)
                                                                                                                     tGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCgg                               >  1:5324889/1‑150 (MQ=255)
                                                                                                                         tCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTg                           >  1:8198241/1‑150 (MQ=255)
                                                                                                                          cGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGc                          <  1:2408786/150‑1 (MQ=255)
                                                                                                                           gAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGcc                         <  1:6617862/150‑1 (MQ=255)
                                                                                                                             aCAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCga                       >  1:5559125/1‑150 (MQ=255)
                                                                                                                              cAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCgaa                      <  1:443137/150‑1 (MQ=255)
                                                                                                                              cAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCgaa                      <  1:8741528/150‑1 (MQ=255)
                                                                                                                                aGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCgaaga                    <  2:8239409/150‑1 (MQ=255)
                                                                                                                                 gagTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGAACCAGTGCGGTCTGCCgaagaa                   >  2:3074901/3‑150 (MQ=255)
                                                                                                                                 gCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCgaagaa                   <  1:4995556/150‑1 (MQ=255)
                                                                                                                                 gCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCgaagaa                   >  1:1354479/1‑150 (MQ=255)
                                                                                                                                  cGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCGaagaag                  <  2:8464831/150‑1 (MQ=255)
                                                                                                                                    tCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCGAAGAAGAt                >  2:2231682/1‑150 (MQ=255)
                                                                                                                                    tCCGCTGAAGTCCCTGGCCGACATGATCAAAGGAAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACAGTAGTTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCGAAGAAGAt                >  1:9019157/1‑150 (MQ=255)
                                                                                                                                     ccGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCGAAGAAGATg               <  2:1077544/150‑1 (MQ=255)
                                                                                                                                      cGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCGAAGAAGATgg              <  1:5629015/150‑1 (MQ=255)
                                                                                                                                       gCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCGAAGAAGATGGc             <  2:7150455/150‑1 (MQ=255)
                                                                                                                                        cTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCGAAGAAGATGGcc            >  1:2985675/1‑150 (MQ=255)
                                                                                                                                         tGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCGAAGAAGATGGccc           >  2:8595740/1‑150 (MQ=255)
                                                                                                                                          gAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCGAAGAAGATGGCCCt          >  1:8142061/1‑150 (MQ=255)
                                                                                                                                                ccTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCGAAGAAGATGGCCCTCGAGCt    <  2:4784368/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCGAAGAAGATGGCCCTCGAGCTGT  >  NC_002947/542597‑542892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑