Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 542,745 G→T 8.2% A328S (GCC→TCC)  rpoC → DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947542,7450GT 8.2% 944.0 / 51.4 287A328S (GCC→TCC) rpoCDNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base G (120/143);  new base T (17/7);  total (137/150)
Fisher's exact test for biased strand distribution p-value = 1.97e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 289 total aligned reads displayed.

TCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCGAAGAAGATGGCCCTCGAGCTGTTC  >  NC_002947/542597‑542894
                                                                                                                                                    |                                                                                                                                                     
      gcgcCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACAt                                                                                                                                                >  2:6617862/1‑150 (MQ=255)
        gcCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGa                                                                                                                                              >  2:9277/1‑150 (MQ=255)
        gcCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGa                                                                                                                                              >  2:8171127/1‑150 (MQ=255)
        gcCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGa                                                                                                                                              <  2:5323894/150‑1 (MQ=255)
        gcCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGa                                                                                                                                              >  2:5125305/1‑150 (MQ=255)
           tgctgGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCa                                                                                                                                           >  1:3819900/1‑150 (MQ=255)
            gctgGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCaa                                                                                                                                          >  2:2498884/1‑150 (MQ=255)
            gctgGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCaa                                                                                                                                          >  1:5121804/1‑150 (MQ=255)
              tgGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAg                                                                                                                                        <  1:8935444/150‑1 (MQ=255)
              tgGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGACCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAg                                                                                                                                        <  1:7153805/150‑1 (MQ=255)
                gATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGt                                                                                                                                      >  1:1291067/1‑150 (MQ=255)
                  tCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTaa                                                                                                                                    <  1:6398105/150‑1 (MQ=255)
                   cTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAg                                                                                                                                   >  2:3805594/1‑150 (MQ=255)
                     gTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCa                                                                                                                                 >  2:4995556/1‑150 (MQ=255)
                           gcCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCg                                                                                                                           >  2:1714682/1‑150 (MQ=255)
                           gcCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCg                                                                                                                           >  1:2044727/1‑150 (MQ=255)
                              ggACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGttt                                                                                                                        >  1:7602030/1‑150 (MQ=255)
                               gACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTc                                                                                                                       <  1:5270313/150‑1 (MQ=255)
                                aCATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTcc                                                                                                                      <  2:4882724/150‑1 (MQ=255)
                                aCATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTcc                                                                                                                      <  1:7984258/150‑1 (MQ=255)
                                    catcGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCa                                                                                                                  <  2:7920104/150‑1 (MQ=255)
                                      tcGTGCGCAACGAACAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGa                                                                                                                <  1:8273709/150‑1 (MQ=255)
                                      tcGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGa                                                                                                                >  1:6883702/1‑150 (MQ=255)
                                        gTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAAc                                                                                                              <  2:7444377/150‑1 (MQ=255)
                                          gcgcAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACtt                                                                                                            <  1:3434447/150‑1 (MQ=255)
                                            gcAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACCTGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGc                                                                                                          >  1:7396952/1‑150 (MQ=255)
                                            gcAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGGAAGCAAGGTCGTTTCCGTCAGAACTTGc                                                                                                          <  2:6798942/150‑1 (MQ=255)
                                             taaCGAAAAGCGTATGTTGCAGGAAGCTGTTGACGCGCTGTTCGATAACGGCCGTCGTGGCCGCGTCATCACCGGTGCTAACAAGCGTCCGCTGAAGTCGCTCTCCGACATGCTCAAGGGTAAGCAGGGTCGTTTCCGTCAGAAccttct                                                                                                         >  2:395650/2‑145 (MQ=255)
                                             cAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACCTGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCt                                                                                                         >  2:7362854/1‑150 (MQ=255)
                                             cAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCt                                                                                                         <  2:6480220/150‑1 (MQ=255)
                                             cAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCt                                                                                                         <  2:4459899/150‑1 (MQ=255)
                                             cAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGAAAGCAAGTTCGTTTCCGTCAGAACTTGCt                                                                                                         >  1:6765838/1‑150 (MQ=255)
                                              aaCGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTc                                                                                                        >  2:2637960/1‑150 (MQ=255)
                                               aCGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCg                                                                                                       <  1:3293387/150‑1 (MQ=255)
                                                 gAAAAGCGTATGTTGCAGGAAGCTTTTGACGCGCTGTTCGATAACGGCCGTCGTGGCAGCGTCATCACCGGTGCTAACAAGCGTCCGCTGAAGTCGCTCTCAGACATGCTCAAGGGTAAGCAGGGTAGTTTCCGTCAGAAAATTCTCGGt                                                                                                     >  1:4776433/1‑150 (MQ=255)
                                                 gAAAAGCGTATGTTGCAGGAAGCTGTTGACGCGCTGTTCGATAACGGCCGTCGTGGCCGCGTCATCACCGGTGCTAACAAGCGTCCGCTGAAGTCGCTCTCCGACATGCTCAAGGGTAAGCAGGGTCGTTTCCGTCAGAACCTTCTCGGt                                                                                                     >  1:2329894/1‑150 (MQ=255)
                                                 gAAAAGCGTATGTTGCAGGAAGCTGTTGACGCGCTGTTCGATAACGGACGTCGTGGCCGCGTCATCACCGGTGCTAACAAGCGTCCGCTGAAGTCGCTCTCCGACATGCTCAAGGGTAAGCAGGGTCGTTTCCGTCAGAACCTTCTCGGt                                                                                                     >  1:7826563/1‑150 (MQ=255)
                                                 gAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGt                                                                                                     >  1:1444708/1‑150 (MQ=255)
                                                 gAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGt                                                                                                     >  1:4703066/1‑150 (MQ=255)
                                                 gAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGt                                                                                                     <  1:2468531/150‑1 (MQ=255)
                                                 gAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGt                                                                                                     >  2:7701217/1‑150 (MQ=255)
                                                    aaGCGTATGTTGCAGGAAGCTGTTGACGCGCTGTTCGATAACGGCCGTCGTGGCCGCGTCATCACCGGTGCTAACAAGCGTCCGCTGAAGTCGCTCTCCGACATGCTCAAGGGTAAGCAGGGTCGTTTCCGTCAGAACCTTCTCGGTAAg                                                                                                  >  2:2983137/1‑150 (MQ=255)
                                                    aaGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAg                                                                                                  <  1:8615305/150‑1 (MQ=255)
                                                       cgcATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCgt                                                                                               <  1:3678017/150‑1 (MQ=255)
                                                       cgcATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCgt                                                                                               >  1:5850600/1‑150 (MQ=255)
                                                        gcATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCgtg                                                                                              >  2:2154837/1‑150 (MQ=255)
                                                         cATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCgtgt                                                                                             <  1:6549599/150‑1 (MQ=255)
                                                          aTGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGtt                                                                                            <  2:8936232/150‑1 (MQ=255)
                                                            gctgcAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGa                                                                                          <  1:5733535/150‑1 (MQ=255)
                                                              tgcAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGact                                                                                        >  1:5092432/1‑150 (MQ=255)
                                                                  ggAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTc                                                                                    <  1:8347855/150‑1 (MQ=255)
                                                                  ggAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTc                                                                                    >  2:7739151/1‑150 (MQ=255)
                                                                     aGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCgg                                                                                 <  2:7001130/150‑1 (MQ=255)
                                                                      gctgTTGACGCGCTGTTCGATAACGGCCGTCGTGGCCGCGTCATCAACGGTGCTAACAAGCGTCCGCTGAAGTCGCTCTCCGACATGCTCAAGGGTAAGCAGGGTCGTTTCCGTCAGAACCTTCTCGGTAAGCGCGTCGACTATTCgggt                                                                                <  1:8481710/147‑2 (MQ=255)
                                                                      gCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGc                                                                                >  1:6128470/1‑150 (MQ=255)
                                                                        tgTTGACGCGCTGTTCGATAACGGCCGTCGTGGCCGCGTCATCACCGGTGCTAACAAGCGTCCGCTGAAGTCGCTCTCCGACATGCTCAAGGGTAAGCAGGGTCGTTTCCGTCAGAACCTTCTCGGTAAGCGCGTCGACTATTCGGGTCg                                                                              >  1:5725960/2‑150 (MQ=255)
                                                                         gTTGACGCGCTGTTCGATAACGGCCGTCGTGGCCGCGTCATCACCGGTGCTAACAAGCGTCCGCTGAAGTCGCTCTCCGACATGCTCAAGGGTAAGCAGGGTCGTTTCCGTCAGAACCTTCTCGGTAAGCGCGTCGACTATTCGGGTCGt                                                                             >  2:2842571/1‑150 (MQ=255)
                                                                          ttGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGtt                                                                            <  2:6137713/150‑1 (MQ=255)
                                                                             aCGCGCTGTTCGATAACGGCCGTCGTGGCCGCGTCATCACCGGTGCTAACAAGCGTCCGCTGAAGTCGCTCTCCGACATGCTCAAGGGTAAGCAGGGTCGTTTCCGTCAGAACCTTCTCGGTAAGCGCGTCGACTATTCGGGTCGTTCgg                                                                         <  1:7373755/150‑1 (MQ=255)
                                                                             aCGCGCTGTTCGATAACGGCCGTCGTGGCCGCGTCATCACCGGTGCTAACAAGCGTCCGCTGAAGTCGCTCTCCGACATGCTCAAGGGTAAGCAGGGTCGTTTCCGTCAGAACCTTCTCGGTAAGCGCGTCGACTATTCGGGTCGTTCgg                                                                         >  1:6946103/1‑150 (MQ=255)
                                                                             aCGCGCTGTTCGATAACGGCCGTCGTGGCCGCGTCATCACCGGTGCTAACAAGCGTCCGCTGAAGTCGCTCTCCGACATGCTCAAGGGTAAGCAGGGTCGTTTCCGTCAGAACCTTCTCGGTAAGCGCGTCGACTATTCGGGTCGTTCgg                                                                         >  2:5938330/1‑150 (MQ=255)
                                                                                   tgctgGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTa                                                                   >  1:692798/1‑150 (MQ=255)
                                                                                     ctgGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTAcc                                                                 >  2:1826184/1‑150 (MQ=255)
                                                                                     ctgGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTAcc                                                                 >  1:5436415/1‑150 (MQ=255)
                                                                                      tgGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCg                                                                >  2:6296004/1‑150 (MQ=255)
                                                                                        gACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTa                                                              <  2:2227801/150‑1 (MQ=255)
                                                                                         aCAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAg                                                             <  1:3999408/150‑1 (MQ=255)
                                                                                                 cgtcgtGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGAcc                                                     >  1:1112489/1‑150 (MQ=255)
                                                                                                     gtGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGc                                                 <  2:2044727/150‑1 (MQ=255)
                                                                                                     gtGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGc                                                 <  1:2877039/150‑1 (MQ=255)
                                                                                                     gtGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGc                                                 <  1:2786884/150‑1 (MQ=255)
                                                                                                       ggTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGt                                               <  1:4963647/150‑1 (MQ=255)
                                                                                                            gcgaaTCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCACAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTAGGTAATTACCGTAGGTCCGACCCTGAGTCTGCa                                          >  2:5677552/5‑150 (MQ=255)
                                                                                                            gcgaaTCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCa                                          >  2:3763706/5‑150 (MQ=255)
                                                                                                             gcCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCAc                                         <  1:8699711/150‑1 (MQ=255)
                                                                                                               cATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCa                                       >  2:9043911/1‑150 (MQ=255)
                                                                                                               cATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCa                                       <  1:7043933/150‑1 (MQ=255)
                                                                                                                    cTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCg                                  <  1:9277/150‑1 (MQ=255)
                                                                                                                    cTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCg                                  <  1:8171449/150‑1 (MQ=255)
                                                                                                                     tGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTGAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCgg                                 >  1:8959589/1‑150 (MQ=255)
                                                                                                                         tCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTg                             >  1:4867179/1‑150 (MQ=255)
                                                                                                                         tCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTg                             >  1:985657/1‑150 (MQ=255)
                                                                                                                          cGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGc                            <  1:2408786/150‑1 (MQ=255)
                                                                                                                           gAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGcc                           <  1:6617862/150‑1 (MQ=255)
                                                                                                                             aCAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCga                         >  1:5559125/1‑150 (MQ=255)
                                                                                                                              cAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCgaa                        <  1:443137/150‑1 (MQ=255)
                                                                                                                              cAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCgaa                        <  1:8741528/150‑1 (MQ=255)
                                                                                                                                aGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCgaaga                      <  2:8239409/150‑1 (MQ=255)
                                                                                                                                 gagTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGAACCAGTGCGGTCTGCCgaagaa                     >  2:3074901/3‑150 (MQ=255)
                                                                                                                                 gCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCgaagaa                     <  2:1291067/150‑1 (MQ=255)
                                                                                                                                 gCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCgaagaa                     >  1:1354479/1‑150 (MQ=255)
                                                                                                                                  cGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCGaagaag                    <  2:8464831/150‑1 (MQ=255)
                                                                                                                                    tCCGCTGAAGTCCCTGGCCGACATGATCAAAGGAAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACAGTAGTTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCGAAGAAGAt                  >  1:9019157/1‑150 (MQ=255)
                                                                                                                                      cGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCGAAGAAGATgg                <  1:5629015/150‑1 (MQ=255)
                                                                                                                                       gCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCGAAGAAGATGGc               <  2:7150455/150‑1 (MQ=255)
                                                                                                                                        cTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCGAAGAAGATGGcc              >  1:2985675/1‑150 (MQ=255)
                                                                                                                                        cTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCGAAGAAGATGGcc              <  2:230232/150‑1 (MQ=255)
                                                                                                                                         tGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCGAAGAAGATGGccc             >  2:8595740/1‑150 (MQ=255)
                                                                                                                                          gAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCGAAGAAGATGGCCCt            >  1:8142061/1‑150 (MQ=255)
                                                                                                                                                    gCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCGAAGAAGATGGCCCTCGAGCTGTTc  >  1:9064353/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TCTGAAGCGCCTGCTGGATCTGTCGGCGCCGGACATCATCGTGCGCAACGAAAAGCGCATGCTGCAGGAAGCGGTTGACGCTCTGCTGGACAACGGCCGTCGTGGTCGCGCCATCACTGGCTCGAACAAGCGTCCGCTGAAGTCCCTGGCCGACATGATCAAAGGTAAGCAAGGTCGTTTCCGTCAGAACTTGCTCGGTAAGCGTGTTGACTACTCCGGCCGTTCGGTAATTACCGTAGGTCCGACCCTGCGTCTGCACCAGTGCGGTCTGCCGAAGAAGATGGCCCTCGAGCTGTTC  >  NC_002947/542597‑542894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑