BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	549,655	C→T	12.3%	A109A (GCC→GCT)	tufB →	elongation factor Tu‑B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	549,655	0	C	T	12.3%	781.3 / 78.4	285	A109A (GCC→GCT)	tufB	elongation factor Tu‑B
Reads supporting (aligned to +/- strand): ref base C (123/127); new base T (13/22); total (136/149)
Fisher's exact test for biased strand distribution p-value = 2.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.78e-01

Only 100 of 300 total aligned reads displayed.

GTATCACCATCAACACCGCTCACGTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTC  >  NC_002947/549507‑549804

gTATCACCATCAACACCGCTCACGTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGccgccg                                                                                                                                                      >  1:9117942/1‑150 (MQ=32)

   tCACCATCAACACCGCTCACGTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATg                                                                                                                                                   >  2:7441050/1‑150 (MQ=32)

   tCACCATCAACACCGCTCACGTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATg                                                                                                                                                   <  2:2268006/150‑1 (MQ=32)

      ccATCAACACCGCTCACGTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTc                                                                                                                                                >  2:2110848/1‑150 (MQ=32)

      ccATCAACACCGCTCACGTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTc                                                                                                                                                >  2:6462677/1‑150 (MQ=32)

       cATCAACACCGCTCACGTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTcc                                                                                                                                               >  1:292559/1‑150 (MQ=32)

       cATCAACACCGCTCACGTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTcc                                                                                                                                               >  1:4862076/1‑150 (MQ=32)

         tCAACACCGCTCACGTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGa                                                                                                                                             <  1:6758881/150‑1 (MQ=32)

                  cTCACGTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCaaa                                                                                                                                    >  1:7556856/1‑150 (MQ=34)

                   tCACGTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAAc                                                                                                                                   >  2:138330/1‑150 (MQ=34)

                   tCACGTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAAc                                                                                                                                   >  1:6357739/1‑150 (MQ=34)

                     aCGTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAAccc                                                                                                                                 >  2:695597/1‑150 (MQ=34)

                       gTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGt                                                                                                                               <  1:3317777/150‑1 (MQ=34)

                        tCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAAccc                                                                                                                                 >  2:5081910/1‑147 (MQ=34)

                         cGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGa                                                                                                                             <  1:7860861/150‑1 (MQ=34)

                          gAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAg                                                                                                                            <  2:8337379/150‑1 (MQ=34)

                             tACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGcac                                                                                                                         >  2:7215488/1‑150 (MQ=34)

                             tACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGcac                                                                                                                         >  1:5722081/1‑150 (MQ=34)

                                   tCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCctg                                                                                                                   >  1:1904885/1‑150 (MQ=34)

                                   tCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCctg                                                                                                                   >  1:5407390/1‑150 (MQ=34)

                                     gAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCTGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCctgct                                                                                                                 <  1:1387558/150‑1 (MQ=25)

                                     gAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCctgct                                                                                                                 >  1:1044317/1‑150 (MQ=35)

                                     gAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCctgct                                                                                                                 <  2:6153465/150‑1 (MQ=35)

                                     gAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCctgct                                                                                                                 <  1:2300857/150‑1 (MQ=35)

                                             gTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTc                                                                                                         >  2:300453/1‑150 (MQ=34)

                                             gTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTc                                                                                                         >  2:7832093/1‑150 (MQ=34)

                                              tCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCa                                                                                                        <  2:2418622/150‑1 (MQ=34)

                                                aCTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAgg                                                                                                      <  1:1162580/150‑1 (MQ=34)

                                                 cTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGt                                                                                                     >  2:8506456/1‑150 (MQ=34)

                                                     gCTCACGTTGACTGCCCAGGTCCCGCTGACTACGTGAAGAACCTGAGCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTTAGCACATCCTGCTGTCCCGTCAGGTAGGc                                                                                                 <  1:531258/150‑1 (MQ=255)

                                                     gCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGt                                                                                                                               <  2:7235659/120‑1 (MQ=32)

                                                     gCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGc                                                                                                 <  1:5580754/150‑1 (MQ=34)

                                                       tCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGt                                                                                               >  1:129896/1‑150 (MQ=34)

                                                       tCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGt                                                                                               >  1:739225/1‑150 (MQ=34)

                                                       tCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGt                                                                                               >  1:3755956/1‑150 (MQ=34)

                                                        cagtttGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCAACGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGtt                                                                                              <  2:3911718/146‑1 (MQ=21)

                                                         aCGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTc                                                                                             >  2:4188034/1‑150 (MQ=34)

                                                            ttGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGt                                                                                          <  1:137102/150‑1 (MQ=34)

                                                            ttGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGt                                                                                          <  1:7220982/150‑1 (MQ=34)

                                                            tcgACTGCCCAGGTCACGCTGACTATGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCAATTCTGGTTGTTTCGGCCGCTGACGGCCCGATGCCGCAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCgg                                                                                          >  2:2510596/3‑149 (MQ=11)

                                                               aCTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACa                                                                                       <  2:4042936/150‑1 (MQ=34)

                                                                 tGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATc                                                                                     >  2:5405233/1‑150 (MQ=34)

                                                                 tGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATc                                                                                     >  1:3281451/1‑150 (MQ=34)

                                                                 tGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATc                                                                                     >  1:3949844/1‑150 (MQ=34)

                                                                 tGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATc                                                                                     <  1:2345794/150‑1 (MQ=34)

                                                                   cccAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGt                                                                                   >  2:3588863/1‑150 (MQ=34)

                                                                    ccAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTg                                                                                  <  1:8494474/150‑1 (MQ=34)

                                                                       ggTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTc                                                                               <  1:1615657/150‑1 (MQ=34)

                                                                         tCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCtt                                                                             >  1:2494018/1‑150 (MQ=34)

                                                                          cACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTc                                                                            >  2:1450225/1‑150 (MQ=34)

                                                                          cACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTc                                                                            <  1:5141944/150‑1 (MQ=34)

                                                                           aCGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGCCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTcc                                                                           >  2:4821661/1‑150 (MQ=21)

                                                                               tGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGaa                                                                       <  2:7585225/150‑1 (MQ=34)

                                                                                   tACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAg                                                                   >  2:4411801/1‑150 (MQ=34)

                                                                                    aCGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAgg                                                                  <  1:8368571/150‑1 (MQ=34)

                                                                                         aagaaCATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGAc                                                             <  2:3281451/150‑1 (MQ=37)

                                                                                         aagaaCATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGAc                                                             <  2:8738607/150‑1 (MQ=37)

                                                                                           gaaCATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCt                                                           >  1:6602333/1‑150 (MQ=37)

                                                                                            aaCATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTg                                                          <  1:3565731/150‑1 (MQ=37)

                                                                                             aCATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTgg                                                         <  2:6799326/150‑1 (MQ=37)

                                                                                             aCATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTgg                                                         <  1:6126409/150‑1 (MQ=37)

                                                                                              cATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCtt                                                                             >  1:2546408/1‑129 (MQ=35)

                                                                                              cATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCtt                                                                             <  2:2546408/129‑1 (MQ=35)

                                                                                              cATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGt                                                        <  1:976692/150‑1 (MQ=37)

                                                                                               aTGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTa                                                       <  2:2844718/150‑1 (MQ=37)

                                                                                                 gATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAga                                                     >  1:4091758/1‑150 (MQ=37)

                                                                                                 gATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAga                                                     >  1:2708699/1‑150 (MQ=37)

                                                                                                  aTCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAgac                                                    >  1:4723608/1‑150 (MQ=37)

                                                                                                  aTCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAgac                                                    >  1:5067445/1‑150 (MQ=37)

                                                                                                     aCCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAgacgac                                                 >  1:948713/1‑150 (MQ=37)

                                                                                                        ggTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCt                                              <  2:7286893/150‑1 (MQ=37)

                                                                                                        ggTGCTGCACAGATGGACGGCGCGATCGTGGTTGTTTCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCt                                              >  2:3773418/1‑150 (MQ=255)

                                                                                                         gTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTg                                             <  2:6385726/150‑1 (MQ=37)

                                                                                                         gTGCTGCACAGATGGACGGCGCGATCCTGGTTGTTTCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTg                                             <  1:2239806/150‑1 (MQ=2)

                                                                                                            ctgcCCAGATGGACGGCGCAATTCTGGTTGTTTCGGCCGCTGACGGCCCGATGCCGCAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCGGCAATCGTCGTGTTCCTGAACAAGTGCGACCAGGTTGACGATGCAGaac                                          <  2:8173254/150‑3 (MQ=255)

                                                                                                             tgcCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAgct                                         >  1:7566289/1‑150 (MQ=37)

                                                                                                             tgcCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCACCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAgct                                         >  1:2642609/1‑150 (MQ=37)

                                                                                                             tgcACAGATGGACGGCGCGATCCTGGTTGTTTCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAact                                         >  2:5682532/1‑150 (MQ=2)

                                                                                                               cccAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAgctgc                                       >  2:3447684/1‑150 (MQ=37)

                                                                                                                 cAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGctgctg                                     <  2:7859495/150‑1 (MQ=37)

                                                                                                                 cAGATGGACGGCGCGATCCTGGTTGTTTCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAacttctc                                     <  1:2133440/150‑2 (MQ=2)

                                                                                                                 cAGATGGACGGCGCGATCCTGGTTGTTTCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAacttctc                                     <  2:1516064/150‑2 (MQ=2)

                                                                                                                  aGATGGACGGCGCGATCCTGGTTGTTTCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAacttctcg                                    >  1:4248094/1‑148 (MQ=2)

                                                                                                                    aTGGACGGCGCGATCCTGGTTGTTTCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGag                                  >  2:8889838/1‑149 (MQ=2)

                                                                                                                     tGGACGGCGCGATCCTGGTTGTTTCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGagc                                 <  1:8291500/150‑3 (MQ=255)

                                                                                                                            cgcgATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCga                          <  2:8438080/150‑1 (MQ=39)

                                                                                                                            cgcgATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCga                          <  1:448028/150‑1 (MQ=39)

                                                                                                                              cgATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCgaga                        <  2:456249/150‑1 (MQ=39)

                                                                                                                              cgATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCgaga                        <  1:8712345/150‑1 (MQ=39)

                                                                                                                              cgATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCgaga                        <  2:186056/150‑1 (MQ=39)

                                                                                                                               gATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGAt                       >  2:2624993/1‑150 (MQ=39)

                                                                                                                               gATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGAt                       <  2:6506456/150‑1 (MQ=39)

                                                                                                                                 tCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATgg                     <  2:9117942/150‑1 (MQ=39)

                                                                                                                                    tGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAg                  <  2:9033679/150‑1 (MQ=39)

                                                                                                                                            tCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAGCTCGTAGAACTCGAAGTTCGCGaa          <  2:3394626/150‑2 (MQ=255)

                                                                                                                                               gccgccGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACctg       >  2:5035433/1‑150 (MQ=39)

                                                                                                                                               gccgccGATGCTCCGAAGCCGCAAACCCGTGAGCACATCCAGCTGTCCCGACAGGTAGCCGTTCCGTACAACGTGGACTTCCACAACAAGGCCGACCAGGTAGACGACGCTCAGCAGCAGGAACAGGTCGAGAACGAAGTACGCGACctc       >  2:1983624/1‑149 (MQ=255)

                                                                                                                                                  gccgATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACctgctg    <  1:6713929/150‑1 (MQ=39)

                                                                                                                                                  gccgATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGATGCTGGAACTGGTCGAGATGGAAGTTCGCGACctgctg    <  1:7404604/150‑1 (MQ=255)

                                                                                                                                                   ccgATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGt   <  2:2181847/150‑1 (MQ=39)

GTATCACCATCAACACCGCTCACGTCGAGTACAACTCGAACATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTC  >  NC_002947/549507‑549804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`