BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	549,784	G→C	9.4%	M152I (ATG→ATC) ‡	tufB →	elongation factor Tu‑B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	549,784	0	G	C	9.4%	826.3 / 58.7	264	M152I (ATG→ATC) ‡	tufB	elongation factor Tu‑B
Reads supporting (aligned to +/- strand): ref base G (116/123); new base C (14/11); total (130/134)
Fisher's exact test for biased strand distribution p-value = 5.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 272 total aligned reads displayed.

ATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCAAGAAGCTGGTAGAAACTCTGGATGCCTA  >  NC_002947/549635‑549933

aTCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATg                                                                                                                                                       <  2:7888932/150‑1 (MQ=39)

    tGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAg                                                                                                                                                   >  2:2228269/1‑150 (MQ=39)

    tGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAg                                                                                                                                                   <  2:2336545/150‑1 (MQ=39)

    tGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAg                                                                                                                                                   <  2:9033679/150‑1 (MQ=39)

    tGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAg                                                                                                                                                   >  1:2204668/1‑150 (MQ=39)

     ggttgtttCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAGCTCGTAGAACTCGAAGt                                                                                                                                                  <  1:8259573/143‑1 (MQ=255)

     ggTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGACGt                                                                                                                                                  >  2:7264257/1‑150 (MQ=255)

        ttGCTCGGCCGCCGTTGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTcg                                                                                                                                               <  1:8646960/150‑1 (MQ=255)

         tGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTcgc                                                                                                                                              <  2:2081389/150‑1 (MQ=39)

         gtttCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTGCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAACTCGTAGAACTCGAAGGTcgc                                                                                                                                              <  2:8446562/147‑1 (MQ=255)

         gtttCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAACTCGTAGAACTCGAAGTTcgc                                                                                                                                              <  2:7644066/147‑1 (MQ=255)

         gtttCGGCAGCTGACGGCCCGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAACTCGTAGAACTCGAAGTTcgc                                                                                                                                              <  2:185078/147‑1 (MQ=255)

            tCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGAc                                                                                                                                           >  2:4609549/1‑150 (MQ=39)

            tCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGAc                                                                                                                                           <  1:5661586/150‑1 (MQ=39)

            tCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGAc                                                                                                                                           >  1:1637602/1‑150 (MQ=39)

               gccgccGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACctg                                                                                                                                        <  2:4414501/150‑1 (MQ=39)

               gccgccGATGCTCCGAAGCCGCAAACCCGTGAGCACATCCAGCTGTCCCGACAGGTAGCCGTTCCGTACAACGTGGACTTCCACAACAAGGCCGACCAGGTAGACGACGCTCAGCAGCAGGAACAGGTCGAGAACGAAGTACGCGACctc                                                                                                                                        >  2:1983624/1‑149 (MQ=255)

                ccgcagACGGCCCGATGCCGCAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTCGCGTTCCGCCAATCGACGTGTTCCTGAACAATTGCGACCAGGTTGACGATGCAGAACTGCTCGAACTGGTTGAACTGGAAGTACGCGAActtc                                                                                                                                       >  2:1514564/1‑148 (MQ=255)

                  gccgATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACctgctg                                                                                                                                     <  1:6713723/150‑1 (MQ=39)

                      aTGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCa                                                                                                                                 <  2:6357739/150‑1 (MQ=39)

                         gcccGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAGCTCGTAGAACTCGAAGTTCGCGAACTGCTTTcgaagt                                                                                                                              <  2:6855597/148‑6 (MQ=255)

                           ccGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTAc                                                                                                                            <  2:2210104/150‑1 (MQ=39)

                           ccGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAACTCGTAGAACTCGAAGTTCGCGAACTGCTTTcgaagtac                                                                                                                            >  2:3620896/1‑143 (MQ=255)

                           ccGATGCCACAGACCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAACTCGTAGAACTCGAAGTTCGCGAACTGCTTTcgaagtac                                                                                                                            >  2:7351247/1‑143 (MQ=255)

                             gATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTACCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGa                                                                                                                          >  2:2801383/1‑150 (MQ=255)

                                 ccGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTc                                                                                                                      <  2:7536398/150‑1 (MQ=39)

                                   gCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTccc                                                                                                                    >  2:507426/1‑150 (MQ=38)

                                   acagaCCCGTGAGCACATCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAACTCGTAGAACTCGAAGTTCGCGAACTGCTTTCGAAGTACGACTTccc                                                                                                                    >  2:7569529/5‑150 (MQ=255)

                                       aCCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGc                                                                                                                <  2:9157025/150‑1 (MQ=37)

                                       aCCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGc                                                                                                                >  2:497521/1‑150 (MQ=37)

                                            tGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAGGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGcgacga                                                                                                           >  1:6169459/1‑150 (MQ=37)

                                                cacaTCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACt                                                                                                       >  1:6625894/1‑150 (MQ=37)

                                                cacaTCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACt                                                                                                       <  2:4060865/150‑1 (MQ=37)

                                                 acaTCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTc                                                                                                      <  1:2110848/150‑1 (MQ=37)

                                                  caTCCTGCTCGCTCGTCAGGTTGGCGTTCCTGCGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAACTCGTAGAACTCGAAGTTCGCGAACTGCTTTCGAAGTACGACTTCCCAGGcgacgaagttcc                                                                                                     >  1:8670112/1‑144 (MQ=2)

                                                   aTCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATgg                                                                                                                                                      <  1:6246205/100‑1 (MQ=40)

                                                   aTCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCg                                                                                                    >  2:2772387/1‑150 (MQ=37)

                                                   aTCCTGCTCGCTCGTCAGGTTGGCGTTCCTGTGATCGTCGTGTTCCTGAACAAGTGCGATCAGGTTGACGATGCTGAACTTCTCGAACTCGTAGAACTCGAAGTTCGCGAACTGCTTTCGAAGTACGACTTCCCAGGcgacgaagttcca                                                                                                    >  1:1402155/1‑143 (MQ=2)

                                                    tCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGaa                                                                                                                                                    >  1:2775675/1‑101 (MQ=40)

                                                    tCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGcgac                                                                                                             <  1:8547787/140‑1 (MQ=37)

                                                      ctgctgTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGatc                                                                                                 <  1:9083041/150‑1 (MQ=37)

                                                       tgctgTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGaca                                                                                                         >  2:6151655/1‑141 (MQ=37)

                                                       tgctgTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGatca                                                                                                >  1:706768/1‑150 (MQ=37)

                                                         ctgTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACCAGGCCGCCCTGGTAGACGACGCTGAGCTGCTGGATCTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCGAGGCGACGACACTCCGatcatc                                                                                              <  2:270287/150‑1 (MQ=255)

                                                            tCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGatcatcatc                                                                                           <  1:2440496/150‑1 (MQ=34)

                                                              ccGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCgg                                                                                         >  1:2079979/1‑150 (MQ=34)

                                                                 tCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTc                                                                                      <  1:8346558/150‑1 (MQ=34)

                                                                     gTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCt                                                                                  >  1:8752944/1‑150 (MQ=34)

                                                                     gTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCt                                                                                  >  2:5388488/1‑150 (MQ=34)

                                                                      tAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTc                                                                                 <  1:7215488/150‑1 (MQ=34)

                                                                         gCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTa                                                                              <  1:300453/150‑1 (MQ=34)

                                                                          cGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTAt                                                                             >  1:6458716/1‑150 (MQ=34)

                                                                               cGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGccc                                                                        >  2:6262049/1‑150 (MQ=35)

                                                                               cGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGccc                                                                        <  1:7832265/150‑1 (MQ=35)

                                                                                gTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCt                                                                       >  1:7424683/1‑150 (MQ=37)

                                                                                 tACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTg                                                                      <  2:7556856/150‑1 (MQ=37)

                                                                                   cATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGa                                                                    <  1:5057840/150‑1 (MQ=37)

                                                                                    aTCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGaa                                                                   <  2:5608514/150‑1 (MQ=37)

                                                                                    aTCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGaa                                                                   <  2:3755956/150‑1 (MQ=37)

                                                                                      cGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAgg                                                                 <  1:4530389/150‑1 (MQ=37)

                                                                                             ttCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAgac                                                          >  2:5936212/1‑150 (MQ=37)

                                                                                              tCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAgacg                                                         >  2:2053171/1‑150 (MQ=37)

                                                                                               ccTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAgacga                                                        <  2:4823798/150‑1 (MQ=37)

                                                                                               ccTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAgacga                                                        <  1:3574197/150‑1 (MQ=37)

                                                                                               ccTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAgacga                                                        >  2:2904226/1‑150 (MQ=37)

                                                                                                 tGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACa                                                      >  1:376227/1‑150 (MQ=37)

                                                                                                   aaCAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAAc                                                    <  2:7714569/150‑1 (MQ=37)

                                                                                                   aaCAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAAc                                                    >  1:2296396/1‑150 (MQ=37)

                                                                                                   aaCAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAAc                                                    >  2:2908185/1‑150 (MQ=37)

                                                                                                     cAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGa                                                  >  1:5354078/1‑150 (MQ=37)

                                                                                                     cAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGa                                                  >  1:6715511/1‑150 (MQ=37)

                                                                                                      aaGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGaa                                                 >  1:4894685/1‑150 (MQ=37)

                                                                                                         gCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATg                                              >  1:4328804/1‑150 (MQ=37)

                                                                                                         gCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATg                                              >  1:4787195/1‑150 (MQ=37)

                                                                                                          ccGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATgg                                             <  2:1044317/150‑1 (MQ=35)

                                                                                                               cTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACt                                        <  1:3410639/150‑1 (MQ=34)

                                                                                                                 ggTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTAc                                      >  2:7409939/1‑150 (MQ=34)

                                                                                                                  gTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTAcc                                     >  1:3993458/1‑150 (MQ=34)

                                                                                                                     gacgacGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCt                                  <  2:948713/150‑1 (MQ=34)

                                                                                                                     gacgacGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCt                                  <  1:4057228/150‑1 (MQ=34)

                                                                                                                       cgacgCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGt                                <  2:5260331/150‑1 (MQ=34)

                                                                                                                       cgacgCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGt                                <  2:129896/150‑1 (MQ=34)

                                                                                                                        gacgCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTc                               >  2:2556040/1‑150 (MQ=35)

                                                                                                                         acgCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCa                              <  2:3310586/150‑1 (MQ=35)

                                                                                                                                 ctgctgGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCAAGAAGCTg                      <  2:706768/150‑1 (MQ=37)

                                                                                                                                 ctgctgGAACTGGTCGAGAGGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCAAGAAGCTg                      <  1:7264257/150‑1 (MQ=37)

                                                                                                                                     tgGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCAAGAAGCTGGTAg                  >  1:2395492/1‑150 (MQ=37)

                                                                                                                                       gAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCAAGAAGCTGGTAGaa                >  1:6055843/1‑150 (MQ=37)

                                                                                                                                        aaCTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCGTCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCAAGAAGCTGGTAGaaa               <  1:5080771/150‑1 (MQ=25)

                                                                                                                                             gTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCAAGAAGCTGGTAGAAACTCTg          <  2:4091758/150‑1 (MQ=34)

                                                                                                                                             gTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCAAGAAGCTGGTAGAAACTCTg          >  1:5714961/1‑150 (MQ=34)

                                                                                                                                             gTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCAAGAAGCTGGTAGAAACTCTg          <  2:1308842/150‑1 (MQ=34)

                                                                                                                                             gTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCAAGAAGCTGGTAGAAACTCTg          <  2:1684167/150‑1 (MQ=34)

                                                                                                                                              tCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCAAGAAGCTGGTAGAAACTCTgg         <  2:2642609/150‑1 (MQ=34)

                                                                                                                                              tCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCAAGAAGCTGGTAGAAACTCTgg         >  2:3424747/1‑150 (MQ=34)

                                                                                                                                                gagaTGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCAAGAAGCTGGTAGAAACTCTGGAt       >  1:3178218/1‑150 (MQ=35)

                                                                                                                                                gagaTGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCAAGAAGCTGGTAGAAACTCTGGAt       >  2:3700885/1‑150 (MQ=35)

                                                                                                                                                gagaTGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCAAGAAGCTGGTAGAAACTCTGGAt       >  1:1331855/1‑150 (MQ=35)

                                                                                                                                                  gaTGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCAAGAAGCTGGTAGAAACTCTGGATGc     <  1:5035433/150‑1 (MQ=37)

                                                                                                                                                     ggAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCAAGAAGCTGGTAGAAACTCTGGATGCCTa  >  2:2443847/1‑150 (MQ=39)

ATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCGCAAACCCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTCTTCCTGAACAAGGCCGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGACCTGCTGTCCACCTACGACTTCCCAGGCGACGACACTCCGATCATCATCGGTTCCGCTCGTATGGCCCTGGAAGGCAAAGACGACAACGAAATGGGCACTACCGCTGTCAAGAAGCTGGTAGAAACTCTGGATGCCTA  >  NC_002947/549635‑549933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`