BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	554,501	G→C	8.3%	A146A (GCG→GCC)	rpsC →	30S ribosomal protein S3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	554,501	0	G	C	8.3%	1219.9 / 74.8	372	A146A (GCG→GCC)	rpsC	30S ribosomal protein S3
Reads supporting (aligned to +/- strand): ref base G (179/162); new base C (19/12); total (198/174)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

Only 100 of 389 total aligned reads displayed.

CGTCAGGACCTGACCAAGCAGATGGGTGTGCCTGTGCACATCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTA  >  NC_002947/554352‑554650

 gTCAGGACCTGACCAAGCAGATGGGTGTGCCTGTGCACATCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCgg                                                                                                                                                      <  2:4521008/150‑1 (MQ=255)

 gTCAGGACCTGACCAAGCAGATGGGTGTGCCTGTGCACATCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCgg                                                                                                                                                      <  1:3500807/150‑1 (MQ=255)

 gTCAGGACCTGACCAAGCAGATGGGTGTGCCTGTGCACATCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCgg                                                                                                                                                      <  1:1749661/150‑1 (MQ=255)

  tCAGGACCTGACCAAGCAGATGGGTGTGCCTGTGCACATCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGt                                                                                                                                                     >  1:6925085/1‑150 (MQ=255)

    aGGACCTGACCAAGCAGATGGGTGTGCCTGTGCACATCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTAc                                                                                                                                                   >  1:1522998/1‑150 (MQ=255)

     ggACCTGACCAAGCAGATGGGTGTGCCTGTGCACATCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACa                                                                                                                                                  >  2:5636007/1‑150 (MQ=255)

     ggACCTGACCAAGCAGATGGGTGTGCCTGTGCACATCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACa                                                                                                                                                  <  2:31379/150‑1 (MQ=255)

      gACCTGACCAAGCAGATGGGTGTGCCTGTGCACATCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAg                                                                                                                                                 >  2:7350100/1‑150 (MQ=255)

              cAAGCAGATGGGTGTGCCTGTGCACATCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCAt                                                                                                                                         >  1:2982050/1‑150 (MQ=255)

               aaGCAGATGGGTGTGCCTGTGCACATCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATg                                                                                                                                        >  2:5178598/1‑150 (MQ=255)

                aGCAGATGGGTGTGCCTGTGCACATCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGc                                                                                                                                       <  1:3618647/150‑1 (MQ=255)

                        ggTGTGCCTGTGCACATCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGt                                                                                                                               <  2:611244/150‑1 (MQ=255)

                         gtgtGCCTGTGCACATCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTg                                                                                                                              >  2:383306/1‑150 (MQ=255)

                           gtgCCTGTGCACATCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGcc                                                                                                                            >  1:4782588/1‑150 (MQ=255)

                             gCCTGTGCACATCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCaa                                                                                                                          <  1:9097594/150‑1 (MQ=255)

                             gCCTGTGCACATCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCaa                                                                                                                          <  1:4470718/150‑1 (MQ=255)

                             gCCTGTGCACATCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCaa                                                                                                                          <  2:4446127/150‑1 (MQ=255)

                              ccTGTGCACATCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCGGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAg                                                                                                                         <  1:3814068/150‑1 (MQ=255)

                               cTGTGCACATCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAgg                                                                                                                        <  2:3645939/150‑1 (MQ=255)

                               cTGTGCACATCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAgg                                                                                                                        >  2:5996812/1‑150 (MQ=255)

                               cTGTGCACATCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAgg                                                                                                                        >  2:6567725/1‑150 (MQ=255)

                                tgtgCACATCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAggg                                                                                                                       >  1:8889900/1‑150 (MQ=255)

                                  tgCACATCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCa                                                                                                                     >  1:5582899/1‑150 (MQ=255)

                                      caTCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCaa                                                                                                                 <  1:5057088/150‑1 (MQ=255)

                                       aTCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAg                                                                                                                >  2:6185808/1‑150 (MQ=255)

                                        tCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGa                                                                                                               <  2:3078509/150‑1 (MQ=255)

                                        tCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGa                                                                                                               <  1:4540044/150‑1 (MQ=255)

                                        tCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGa                                                                                                               <  1:4045821/150‑1 (MQ=255)

                                         cAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGAt                                                                                                              >  1:4879948/1‑150 (MQ=255)

                                          aaCATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATc                                                                                                             >  2:7041785/1‑150 (MQ=255)

                                             aTCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAg                                                                                                          >  1:7727393/1‑150 (MQ=255)

                                             aTCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAg                                                                                                          <  2:6926149/150‑1 (MQ=255)

                                             aTCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAg                                                                                                          <  1:100890/150‑1 (MQ=255)

                                                gAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTg                                                                                                       >  2:2698129/1‑150 (MQ=255)

                                                gAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTg                                                                                                       >  2:2534296/1‑150 (MQ=255)

                                                gAAGAGATCCGCAAGCCAGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATAAATATCAAGGTg                                                                                                       >  1:5299767/1‑150 (MQ=255)

                                                 aaGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGa                                                                                                      <  1:2654944/150‑1 (MQ=255)

                                                 aaGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGa                                                                                                      <  1:6579479/150‑1 (MQ=255)

                                                 aaGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGa                                                                                                      <  2:3272731/150‑1 (MQ=255)

                                                  agagATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAg                                                                                                     <  2:1978999/150‑1 (MQ=255)

                                                  agagATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAg                                                                                                     >  1:606445/1‑150 (MQ=255)

                                                    agaTCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCg                                                                                                   <  2:4427105/150‑1 (MQ=255)

                                                    agaTCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCg                                                                                                   <  2:7634405/150‑1 (MQ=255)

                                                       tCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGgtc                                                                                                <  2:1229216/150‑1 (MQ=255)

                                                         cGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGgtcgt                                                                                              >  1:741386/1‑150 (MQ=255)

                                                           cAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGtct                                                                                            >  1:2828048/1‑150 (MQ=255)

                                                           cAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGtct                                                                                            >  2:8302562/1‑150 (MQ=255)

                                                           cAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGtct                                                                                            >  2:5835902/1‑150 (MQ=255)

                                                              gCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTcgg                                                                                         <  2:7889750/150‑1 (MQ=255)

                                                                   aaCTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTg                                                                                    <  1:3099928/150‑1 (MQ=255)

                                                                      tCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTg                                                                                 >  1:3272111/1‑150 (MQ=255)

                                                                       cGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTga                                                                                <  1:2158215/150‑1 (MQ=255)

                                                                        gACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTgag                                                                               >  2:5240185/1‑150 (MQ=255)

                                                                        gACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTgag                                                                               >  1:1271972/1‑150 (MQ=255)

                                                                         aCGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTgaga                                                                              <  1:6162773/150‑1 (MQ=255)

                                                                           gCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGAtt                                                                            >  2:4252420/1‑150 (MQ=255)

                                                                                   ggTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGc                                                                                                                                       <  2:7544895/83‑1 (MQ=255)

                                                                                    gTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTAcc                                                                   >  1:5888810/1‑150 (MQ=255)

                                                                                     ttGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCg                                                                  >  2:2299772/1‑150 (MQ=255)

                                                                                         gcAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTg                                                              <  2:1022154/150‑1 (MQ=255)

                                                                                         gcAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTg                                                              <  2:7873636/150‑1 (MQ=255)

                                                                                          cAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTgg                                                             <  2:348477/150‑1 (MQ=255)

                                                                                                  aGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGa                                                     >  1:1685392/1‑150 (MQ=255)

                                                                                                   gCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGaa                                                    <  2:8565570/150‑1 (MQ=255)

                                                                                                   gCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGaa                                                    <  2:8889865/150‑1 (MQ=255)

                                                                                                    cTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCt                                                                         <  2:1822878/128‑1 (MQ=255)

                                                                                                    cTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAg                                                   >  2:1896246/1‑150 (MQ=255)

                                                                                                    cTCAGCAGCTAGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCt                                                                                  <  1:6446061/119‑1 (MQ=255)

                                                                                                     tCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAgg                                                  >  2:3957697/1‑150 (MQ=255)

                                                                                                     tCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAgg                                                  >  2:7147407/1‑150 (MQ=255)

                                                                                                     tCAGCAGCTCGAACGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAgg                                                  >  1:4382578/1‑150 (MQ=255)

                                                                                                         cagcTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCgt                                              <  1:191395/150‑1 (MQ=255)

                                                                                                            ctcgAACGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCCGCGCTGAAATCGCACGTAAGGAATGGTATCGCGAAGGTCgcgat                                           >  1:7175049/4‑146 (MQ=255)

                                                                                                             tcgAACGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGACTGGTATCGCGAAGGTCgcgttc                                          >  1:7560439/3‑148 (MQ=255)

                                                                                                               gAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTAc                                                                    >  2:6436829/1‑122 (MQ=255)

                                                                                                               gAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCct                                        <  2:1451301/150‑1 (MQ=255)

                                                                                                                 aCGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTTGTGTGcagag                                      >  2:5388717/1‑146 (MQ=255)

                                                                                                                    ccGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGca                                   >  2:7801032/1‑150 (MQ=255)

                                                                                                                         tggcattCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCacacgc                              >  2:2871986/6‑148 (MQ=255)

                                                                                                                          ggcattCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCt                             <  1:8199439/146‑1 (MQ=255)

                                                                                                                            tGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGc                           <  2:4883358/150‑1 (MQ=255)

                                                                                                                            cattCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGc                           <  2:8196387/148‑1 (MQ=255)

                                                                                                                            cattCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGc                           <  1:654689/148‑1 (MQ=255)

                                                                                                                             gTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCg                          >  2:4231423/1‑150 (MQ=255)

                                                                                                                             gTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCg                          >  2:7247465/1‑150 (MQ=255)

                                                                                                                              ttCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGc                                          <  1:517454/133‑1 (MQ=255)

                                                                                                                              ttCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGCGt                         >  1:6728003/1‑150 (MQ=255)

                                                                                                                                ccGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGc                       <  2:5262709/150‑1 (MQ=255)

                                                                                                                                 cGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGcc                      <  1:5706758/150‑1 (MQ=255)

                                                                                                                                 cGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGcc                      <  2:510548/150‑1 (MQ=255)

                                                                                                                                     gcgcCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGata                  >  1:1328605/1‑150 (MQ=255)

                                                                                                                                      cgcCCTGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCACGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGatat                 <  2:6448277/150‑1 (MQ=255)

                                                                                                                                         cATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGa              <  1:5382181/150‑1 (MQ=255)

                                                                                                                                         cATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGa              <  1:2550546/150‑1 (MQ=255)

                                                                                                                                         cATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGa              >  1:1102907/1‑150 (MQ=255)

                                                                                                                                           tGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACt            <  2:6703248/150‑1 (MQ=255)

                                                                                                                                           tGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACt            <  1:8746069/150‑1 (MQ=255)

                                                                                                                                             aaGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTac          >  1:7494066/1‑150 (MQ=255)

                                                                                                                                                    cGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCt   <  1:2356378/150‑1 (MQ=255)

                                                                                                                                                     ggTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTa  <  2:7727393/150‑1 (MQ=255)

CGTCAGGACCTGACCAAGCAGATGGGTGTGCCTGTGCACATCAACATCGAAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTA  >  NC_002947/554352‑554650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`