BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	554,550	A→T	9.3%	S163C (AGC→TGC) ‡	rpsC →	30S ribosomal protein S3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	554,550	0	A	T	9.3%	1039.6 / 73.3	333	S163C (AGC→TGC) ‡	rpsC	30S ribosomal protein S3
Reads supporting (aligned to +/- strand): ref base A (146/156); new base T (19/12); total (165/168)
Fisher's exact test for biased strand distribution p-value = 1.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

Only 100 of 342 total aligned reads displayed.

AAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTC  >  NC_002947/554401‑554699

aaGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGa                                                                                                                                                       <  1:6579479/150‑1 (MQ=255)

aaGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGa                                                                                                                                                       <  1:2654963/150‑1 (MQ=255)

aaGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGa                                                                                                                                                       >  2:6536938/1‑150 (MQ=255)

 agagATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAg                                                                                                                                                      <  2:1978999/150‑1 (MQ=255)

   agaTCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCg                                                                                                                                                    <  2:1868344/150‑1 (MQ=255)

   agaTCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCg                                                                                                                                                    <  2:7634486/150‑1 (MQ=255)

     aTCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGgt                                                                                                                                                  >  1:3580784/1‑150 (MQ=255)

     aTCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGgt                                                                                                                                                  >  1:4423423/1‑150 (MQ=255)

       ccGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGgtcg                                                                                                                                                >  2:3500781/1‑150 (MQ=255)

        cGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGgtcgt                                                                                                                                               >  1:7566345/1‑150 (MQ=255)

          cAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGtct                                                                                                                                             >  2:5835902/1‑150 (MQ=255)

          cAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGtct                                                                                                                                             >  1:2828048/1‑150 (MQ=255)

            aGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTcg                                                                                                                                           >  1:455508/1‑150 (MQ=255)

            aGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTcg                                                                                                                                           <  1:3837909/150‑1 (MQ=255)

             gCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTcgg                                                                                                                                          <  2:7889750/150‑1 (MQ=255)

               cGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTcggcg                                                                                                                                        >  2:6799596/1‑150 (MQ=255)

                     tCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTg                                                                                                                                  >  1:3272111/1‑150 (MQ=255)

                     tCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTg                                                                                                                                  >  1:8149972/1‑150 (MQ=255)

                     tCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTg                                                                                                                                  >  1:1496441/1‑150 (MQ=255)

                     tCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTg                                                                                                                                  >  1:1681029/1‑150 (MQ=255)

                      cGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTga                                                                                                                                 <  1:2158215/150‑1 (MQ=255)

                      cGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTga                                                                                                                                 <  1:5724908/150‑1 (MQ=255)

                      cGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTga                                                                                                                                 <  2:392/150‑1 (MQ=255)

                       gACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTgag                                                                                                                                >  1:1271972/1‑150 (MQ=255)

                       gACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTgag                                                                                                                                >  2:4460200/1‑150 (MQ=255)

                       gACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTgag                                                                                                                                >  2:6398428/1‑150 (MQ=255)

                       gACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTgag                                                                                                                                >  1:5483798/1‑150 (MQ=255)

                        aCGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTgaga                                                                                                                               <  2:7727881/150‑1 (MQ=255)

                            tATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGc                                                                                                                           >  1:8993774/1‑150 (MQ=255)

                              tGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTc                                                                                                                         >  1:7531268/1‑150 (MQ=255)

                                cTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTCCTGAGATTGCTCGt                                                                                                                       >  1:6676876/1‑150 (MQ=255)

                                   gTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTAcc                                                                                                                    >  1:5888810/1‑150 (MQ=255)

                                     tGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGa                                                                                                                  <  1:1153504/150‑1 (MQ=255)

                                     tGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCACCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGa                                                                                                                  <  1:1726768/150‑1 (MQ=255)

                                        gcAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTg                                                                                                               <  1:2617979/150‑1 (MQ=255)

                                                  gCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGaa                                                                                                     <  2:8889900/150‑1 (MQ=255)

                                                  gCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGaa                                                                                                     <  2:1363752/150‑1 (MQ=255)

                                                   cTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCt                                                                                                                          <  2:1822878/128‑1 (MQ=255)

                                                       gcagcTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCg                                                                                                >  2:7803381/1‑150 (MQ=255)

                                                       gcagcTCGAACGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCg                                                                                                >  1:7483754/1‑150 (MQ=255)

                                                         agcTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCgtg                                                                                              <  1:8288523/150‑1 (MQ=255)

                                                           ctcgAACGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCgcgtt                                                                                            >  2:7209842/4‑149 (MQ=255)

                                                           ctcgAACGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCCGCGCTGAAATCGCACGTAAGGAATGGTATCGCGAAGGTCgcgat                                                                                            >  1:7175049/4‑146 (MQ=255)

                                                             ggAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGcc                                                                                          >  2:4437608/1‑150 (MQ=255)

                                                              gAACGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTccg                                                                                         >  2:8313519/1‑149 (MQ=255)

                                                              gAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTAc                                                                                                                     <  1:6436829/122‑1 (MQ=255)

                                                               aaCGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTccgc                                                                                        <  2:1003817/150‑3 (MQ=255)

                                                                aCGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGACTGGTATCGCGAAGGTCGCGTTCcgct                                                                                       >  1:6900256/1‑150 (MQ=255)

                                                                aCGCCGTGTGGCATTCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCcgct                                                                                       >  2:4238227/1‑150 (MQ=255)

                                                                aCGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGCCCGCCATGCGTCTTGGTGCCAAGGGCATCACGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCctct                                                                                       <  2:7608383/150‑1 (MQ=255)

                                                                aCGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTTGTGTGcagag                                                                                       >  2:5388717/1‑146 (MQ=255)

                                                                      tgtAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCacac                                                                                 <  1:735199/150‑1 (MQ=255)

                                                                        tAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACAccc                                                                               >  1:5300279/1‑150 (MQ=255)

                                                                         ggcattCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCt                                                                              <  2:668120/146‑1 (MQ=255)

                                                                         ggcattCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCt                                                                              <  2:5241660/146‑1 (MQ=255)

                                                                           tGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGc                                                                            <  1:5151385/150‑1 (MQ=255)

                                                                           cattCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGc                                                                            <  2:8196387/148‑1 (MQ=255)

                                                                            gTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCg                                                                           >  2:4231342/1‑150 (MQ=255)

                                                                            attCCGTCGCGCCATGAAGCGTGCCGTTCAGTCGGCAATGCGTCTCGGTGCTGAAGGTATCCGTATCAACTGCTCCGGTCGTCTTGGCGGCGCTGAAATCGCACGTATGGAATGGTATCGCGAAGGTCGCGTTCCGCTTCACACGCTGCg                                                                           >  1:611220/2‑150 (MQ=255)

                                                                             ttCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGc                                                                                           >  2:517454/1‑133 (MQ=255)

                                                                             ttCCGTCGCGCCATGAAGCGAGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGt                                                                          <  2:8569376/150‑1 (MQ=255)

                                                                               ccGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGc                                                                        <  2:5262709/150‑1 (MQ=255)

                                                                                  tCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGa                                                                     <  2:2243454/150‑1 (MQ=255)

                                                                                   cgcgCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGat                                                                    <  2:1604712/150‑1 (MQ=255)

                                                                                     cgcCCTGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCACGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGatat                                                                  <  2:6448277/150‑1 (MQ=255)

                                                                                      gcCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATGTc                                                                 >  2:4391600/1‑150 (MQ=255)

                                                                                      gcCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATc                                                                 >  1:9105871/1‑150 (MQ=255)

                                                                                        cATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGa                                                               <  1:2550546/150‑1 (MQ=255)

                                                                                        cATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGa                                                               <  1:2946988/150‑1 (MQ=255)

                                                                                        cATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGa                                                               <  2:7112708/150‑1 (MQ=255)

                                                                                        cATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGa                                                               >  1:1102822/1‑150 (MQ=255)

                                                                                         aTGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGAc                                                              >  1:6793038/1‑150 (MQ=255)

                                                                                          tGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACt                                                             >  2:2508113/1‑150 (MQ=255)

                                                                                                  gCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACAcc                                                     <  2:2779292/150‑1 (MQ=255)

                                                                                                     gTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTAc                                                  <  2:6925354/150‑1 (MQ=255)

                                                                                                          gAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGc                                             >  2:2898863/1‑150 (MQ=255)

                                                                                                               ccATGCGTATTGGTGCCTAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTcaca                                        <  2:6118005/150‑1 (MQ=255)

                                                                                                               ccATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTcaca                                        <  1:40521/150‑1 (MQ=255)

                                                                                                               ccATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTcaca                                        <  1:41761/150‑1 (MQ=255)

                                                                                                                  tGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCAcacca                                     <  1:139940/150‑1 (MQ=255)

                                                                                                                  tGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCAcacca                                     <  1:4971198/150‑1 (MQ=255)

                                                                                                                     gTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGACGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACtt                                  >  1:4328646/1‑150 (MQ=255)

                                                                                                                           gTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGgtg                            >  2:5600519/1‑150 (MQ=255)

                                                                                                                                 aGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCg                      >  2:50615/1‑150 (MQ=255)

                                                                                                                                  gggCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCgg                     <  2:4781050/150‑1 (MQ=255)

                                                                                                                                   ggCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGgt                    <  2:3132413/150‑1 (MQ=255)

                                                                                                                                   ggCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGCGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGgt                    <  1:6185808/150‑1 (MQ=255)

                                                                                                                                   ggCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGAGTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGgt                    <  2:3291531/150‑1 (MQ=255)

                                                                                                                                     cATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGgtgt                  <  2:2982202/150‑1 (MQ=255)

                                                                                                                                      aTCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGtgtg                 <  2:4879948/150‑1 (MQ=255)

                                                                                                                                      aTCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGtgtg                 >  1:8441022/1‑150 (MQ=255)

                                                                                                                                         aaGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAg              >  1:2322725/1‑150 (MQ=255)

                                                                                                                                         aaGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAg              <  1:6486564/150‑1 (MQ=255)

                                                                                                                                          aGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAgg             >  1:560846/1‑150 (MQ=255)

                                                                                                                                             tCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGttt          <  1:5996812/150‑1 (MQ=255)

                                                                                                                                             tCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGttt          <  1:6567725/150‑1 (MQ=255)

                                                                                                                                             tCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGttt          <  1:5194309/150‑1 (MQ=255)

                                                                                                                                             tCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGttt          <  1:7801032/150‑1 (MQ=255)

                                                                                                                                                     aGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTc  <  1:1849656/150‑1 (MQ=255)

                                                                                                                                                     aGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTc  <  2:1271972/150‑1 (MQ=255)

AAGAGATCCGCAAGCCGGAACTCGACGCTATGCTGGTTGCGCAGAGCGTAGCTCAGCAGCTGGAACGCCGTGTAATGTTCCGTCGCGCCATGAAGCGTGCGGTACAGAACGCCATGCGTATTGGTGCCAAGGGCATCAAGATCCAGGTGAGCGGTCGTCTCGGCGGTGCTGAGATTGCTCGTACCGAGTGGTATCGCGAAGGTCGTGTGCCTCTGCACACCCTGCGTGCCGATATCGACTACAACACCTACGAAGCTCACACCACTTACGGTGTGATCGGTGTGAAGGTTTGGATCTTC  >  NC_002947/554401‑554699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`