BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	732,425	A→C	8.5%	V654V (GTA→GTC)	clpB →	chaperone protein ClpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	732,425	0	A	C	8.5%	735.9 / 44.5	232	V654V (GTA→GTC)	clpB	chaperone protein ClpB
Reads supporting (aligned to +/- strand): ref base A (99/113); new base C (8/12); total (107/125)
Fisher's exact test for biased strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

Only 100 of 239 total aligned reads displayed.

GGCCCGACCGGTGTGGGCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG  >  NC_002947/732276‑732574

ggCCCGACCGGTGTGGGCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTa                                                                                                                                                       <  2:2394144/150‑1 (MQ=255)

 gCCCGACCGGTGTGGGCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAg                                                                                                                                                      <  1:8604545/150‑1 (MQ=255)

 gCCCGACCGGTGTGGGCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCAAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAg                                                                                                                                                      <  1:5642969/150‑1 (MQ=255)

  cccGACCGGTGTGGGCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGGGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAgg                                                                                                                                                     <  2:8938237/150‑1 (MQ=255)

  cccGACCGGTGTGGGCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAgg                                                                                                                                                     <  1:8528734/150‑1 (MQ=255)

  cccGACCGGTGTGGGCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAgg                                                                                                                                                     <  1:2200882/150‑1 (MQ=255)

   ccGACCGGTGTGGGCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAggg                                                                                                                                                    <  2:4331640/150‑1 (MQ=255)

    cGACCGGTGTGGGCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGt                                                                                                                                                   <  2:5655760/150‑1 (MQ=255)

        cGGTGTGGGCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGa                                                                                                                                               >  1:5647422/1‑150 (MQ=255)

         ggTGTGGGCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGaa                                                                                                                                              <  2:7200175/150‑1 (MQ=255)

          gtgtGGGCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAag                                                                                                                                             >  1:7748132/1‑150 (MQ=255)

              gggCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAggg                                                                                                                                         <  2:8752915/150‑1 (MQ=255)

                gCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCg                                                                                                                                       <  1:2391871/150‑1 (MQ=255)

                gCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCg                                                                                                                                       <  1:4222463/150‑1 (MQ=255)

                     aCCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTAc                                                                                                                                  <  1:8354777/150‑1 (MQ=255)

                      ccGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTAcc                                                                                                                                 >  1:67909/1‑150 (MQ=255)

                       cGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCt                                                                                                                                <  2:6360827/150‑1 (MQ=255)

                       cGAAATGTGCAAGGCGCTGGCCGAGTTCCTGTTCGAAAACGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGGGAAGCACTCCGTCGCGCGCCTGATCGGTGCACAACCAGGCTATGTAGGTTATGTATATGGCTGTTACCt                                                                                                                                >  2:1288983/1‑150 (MQ=255)

                         aaCTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGa                                                                                                                              <  2:1498937/150‑1 (MQ=255)

                             gtgCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGa                                                                                                                          <  2:1747115/150‑1 (MQ=255)

                              tgCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAg                                                                                                                         <  1:3747847/150‑1 (MQ=255)

                              tgCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAg                                                                                                                         <  1:7133923/150‑1 (MQ=255)

                                     cgcTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTgc                                                                                                                  <  1:415703/150‑1 (MQ=255)

                                         ggCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTgcggcg                                                                                                              >  1:6708377/1‑150 (MQ=255)

                                          gCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGgcgc                                                                                                             >  2:7518381/1‑150 (MQ=255)

                                            cGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCaa                                                                                                           >  2:5825801/1‑150 (MQ=255)

                                              aGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGc                                                                                                         <  2:1836003/150‑1 (MQ=255)

                                                ttCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCt                                                                                                       >  2:6068768/1‑150 (MQ=255)

                                                  ccTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTa                                                                                                     <  2:8165454/150‑1 (MQ=255)

                                                   cTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTAc                                                                                                    <  1:3184286/150‑1 (MQ=255)

                                                   cTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTAc                                                                                                    <  2:3019948/150‑1 (MQ=255)

                                                     gTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTc                                                                                                  >  1:1897390/1‑150 (MQ=255)

                                                        cGACACCGAAGAGGCGATGGTGCGCATCGTCATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCggt                                                                                               >  1:4850753/1‑150 (MQ=255)

                                                        cGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCggt                                                                                               <  2:2640219/150‑1 (MQ=255)

                                                        cGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCggt                                                                                               <  2:3417717/150‑1 (MQ=255)

                                                        cGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCggt                                                                                               <  1:4046656/150‑1 (MQ=255)

                                                         gACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCggtg                                                                                              <  2:2170430/150‑1 (MQ=255)

                                                         gACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCggtg                                                                                              <  2:3601466/150‑1 (MQ=255)

                                                         gACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCggtg                                                                                              <  1:2697167/150‑1 (MQ=255)

                                                              cGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGtgct                                                                                         >  1:6304960/1‑150 (MQ=255)

                                                              cGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGtgct                                                                                         >  1:212283/1‑150 (MQ=255)

                                                               gAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGtgctg                                                                                        >  2:5323297/1‑150 (MQ=255)

                                                               gAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGtgctg                                                                                        >  1:1441427/1‑150 (MQ=255)

                                                                aaGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGtgctgc                                                                                       <  2:9089147/150‑1 (MQ=255)

                                                                 agagGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTgctgct                                                                                      <  2:2264429/150‑1 (MQ=255)

                                                                    ggCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGa                                                                                   <  1:4691432/150‑1 (MQ=255)

                                                                     gCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGAc                                                                                  >  2:4673659/1‑150 (MQ=255)

                                                                        aTGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAg                                                                               <  2:8128938/150‑1 (MQ=255)

                                                                        aTGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAg                                                                               >  2:4135697/1‑150 (MQ=255)

                                                                         tGGTGCGCAACGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAgg                                                                              <  2:8347083/150‑1 (MQ=255)

                                                                            tGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTgg                                                                           >  2:7629523/1‑150 (MQ=255)

                                                                               acATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGaaa                                                                        <  2:8637448/149‑1 (MQ=255)

                                                                                cATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGaaaa                                                                       >  2:6637284/1‑150 (MQ=255)

                                                                                   cGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGc                                                                    <  1:1909250/150‑1 (MQ=255)

                                                                                     aCATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGccc                                                                  <  1:6425320/150‑1 (MQ=255)

                                                                                     aCATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGccc                                                                  >  2:8596529/1‑150 (MQ=255)

                                                                                      cATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCa                                                                 <  1:2468265/150‑1 (MQ=255)

                                                                                       aTGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCAc                                                                >  2:2279442/1‑150 (MQ=255)

                                                                                         gTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCAccc                                                              <  1:4102836/150‑1 (MQ=255)

                                                                                          tCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCg                                                             >  1:4589575/1‑150 (MQ=255)

                                                                                          tCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCg                                                             >  2:6715528/1‑150 (MQ=255)

                                                                                          tCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCg                                                             >  1:357323/1‑150 (MQ=255)

                                                                                          tCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCg                                                             >  1:7397733/1‑150 (MQ=255)

                                                                                            cGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGa                                                           >  1:3499686/1‑150 (MQ=255)

                                                                                             gAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGAt                                                          <  2:4014887/150‑1 (MQ=255)

                                                                                             gAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGAt                                                          <  1:1076046/150‑1 (MQ=255)

                                                                                             gAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGAt                                                          <  2:888586/150‑1 (MQ=255)

                                                                                                 tCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCCCCCGGATgtgt                                                      >  2:6924124/1‑150 (MQ=255)

                                                                                                 tCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATgtgt                                                      >  2:2233501/1‑150 (MQ=255)

                                                                                                   aTGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTc                                                                                                  <  1:361252/104‑1 (MQ=255)

                                                                                                   aTGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTc                                                    >  1:1742086/1‑150 (MQ=255)

                                                                                                         aaGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTg                                              >  1:2614323/1‑150 (MQ=255)

                                                                                                               tCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTg                                        >  1:7760998/1‑150 (MQ=255)

                                                                                                                ccGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGc                                       <  2:5590934/150‑1 (MQ=255)

                                                                                                                 cGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCa                                      >  1:8805439/1‑150 (MQ=255)

                                                                                                                  gTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAg                                     <  1:2642052/150‑1 (MQ=255)

                                                                                                                    gagccgtcTGATTGGTGCACCTCCCGGTTATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGt                                   <  1:9162723/143‑1 (MQ=255)

                                                                                                                     gcgcgcCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTg                                  <  2:3750873/150‑1 (MQ=255)

                                                                                                                      gccgtcTGATTGGTGCACCTCCCGGTTATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGttc                                 <  1:3593135/145‑3 (MQ=255)

                                                                                                                       gcgcCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCt                                <  1:6095662/150‑1 (MQ=255)

                                                                                                                       gcgcCTGATCGGTGCACCACCAGGCAATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCATACACGGTGGTGCTGCTGGACGAGGTGGAAAAAGACCACCCCGATGTGTTAAAAGTGCTGTTTCATGTGCt                                >  1:8596929/1‑150 (MQ=255)

                                                                                                                        cgtcTGATTGGTGCACCTCCCGGTTATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCtt                               <  1:5411381/147‑2 (MQ=255)

                                                                                                                          ccTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAATCCTTACTCGGTGGTGCTGCTGGACGAGGTGTAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGa                             <  1:1288983/150‑1 (MQ=255)

                                                                                                                           cTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGaa                            <  1:2369024/150‑1 (MQ=255)

                                                                                                                           cTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGaa                            <  2:177727/150‑1 (MQ=255)

                                                                                                                            tGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAg                           >  2:4894101/1‑150 (MQ=255)

                                                                                                                            tGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAg                           >  2:386966/1‑150 (MQ=255)

                                                                                                                              aTCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGAc                         <  2:2200625/150‑1 (MQ=255)

                                                                                                                                  gTGCACCTCCCGGTTATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACggtc                     <  2:5980784/150‑3 (MQ=255)

                                                                                                                                      accaccAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGc                                 <  1:3640302/134‑1 (MQ=255)

                                                                                                                                      accaccAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCt                 >  2:5016700/1‑150 (MQ=255)

                                                                                                                                         tcccggttATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGAc              >  1:5377532/8‑150 (MQ=255)

                                                                                                                                         tcccggttATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGAc              >  1:508177/8‑150 (MQ=255)

                                                                                                                                         tcccggttATGTCGGCTATGAAGAGGGTGGCGTTCTGAAGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGAc              >  2:7455129/8‑150 (MQ=255)

                                                                                                                                         accaGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGAc              >  2:2872114/1‑150 (MQ=255)

                                                                                                                                          ccaGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGAcc             <  2:2963930/150‑1 (MQ=255)

                                                                                                                                           caGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCg            >  1:8079580/1‑150 (MQ=255)

                                                                                                                                               cTATGTAGGGTATGAAGAGGGCGGTGACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAg        <  1:8740818/150‑1 (MQ=255)

                                                                                                                                                  tGTAGGGTATGAAGAGGCCGGTTACCTGACCTATGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGACGTGGAAAAAGCCCACCCGGATGAGTTCAACGTGCTGTTGCAGGTGCTGTAAGACGGCAGCCTGACCGACAGccc     >  2:5355103/1‑149 (MQ=255)

                                                                                                                                                    tAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACg   >  2:8823311/1‑150 (MQ=255)

GGCCCGACCGGTGTGGGCAAGACCGAACTGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCGATGGTGCGCATCGACATGTCCGAATTCATGGAGAAGCACTCCGTCGCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG  >  NC_002947/732276‑732574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`