BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	732,542	G→T	11.3%	V693V (GTG→GTT)	clpB →	chaperone protein ClpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	732,542	0	G	T	11.3%	845.1 / 73.3	273	V693V (GTG→GTT)	clpB	chaperone protein ClpB
Reads supporting (aligned to +/- strand): ref base G (135/107); new base T (16/15); total (151/122)
Fisher's exact test for biased strand distribution p-value = 7.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 276 total aligned reads displayed.

GCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGCAGTGATGGATGCGGT  >  NC_002947/732393‑732691

  gcgcCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCt                                                                                                                                                     <  1:6095662/150‑1 (MQ=255)

   cgtcTGATTGGTGCACCTCCCGGTTATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCtt                                                                                                                                                    <  1:5411381/147‑2 (MQ=255)

   cgtcTGATTGGTGCACCTCCCGGTTATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCtt                                                                                                                                                    <  2:7592859/147‑2 (MQ=255)

    gcCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTgg                                                                                                                                                   <  2:2253395/150‑1 (MQ=255)

     tcTGATTGGTGCACCTCCCGGTTATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGa                                                                                                                                                  >  2:3416529/2‑150 (MQ=255)

      cTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGaa                                                                                                                                                 <  1:2369348/150‑1 (MQ=255)

      cTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGaa                                                                                                                                                 <  2:7849356/150‑1 (MQ=255)

       tGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAg                                                                                                                                                >  2:4894101/1‑150 (MQ=255)

           cGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACgg                                                                                                                                            <  2:5338503/150‑1 (MQ=255)

             gTGCACCTCCCGGTTATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACggtc                                                                                                                                          <  1:5144400/150‑3 (MQ=255)

             gTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGgcc                                                                                                                                          >  2:4048676/1‑150 (MQ=255)

                 accaccAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGc                                                                                                                                                      <  1:3640302/134‑1 (MQ=255)

                 accaccAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGc                                                                                                                                                      >  2:3640302/1‑134 (MQ=255)

                 accaccAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCt                                                                                                                                      >  2:5016700/1‑150 (MQ=255)

                    tcccggttATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGAc                                                                                                                                   >  1:5040665/8‑150 (MQ=255)

                    accaGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGAc                                                                                                                                   <  1:2094474/150‑1 (MQ=255)

                        ggttATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCGAc                                                                                                                               >  1:8109158/4‑150 (MQ=255)

                        ggCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGAc                                                                                                                               >  1:1260647/1‑150 (MQ=255)

                          cTATGTAGGGTATGAAGAGGGCGGTGACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAg                                                                                                                             <  1:8740818/150‑1 (MQ=255)

                                aGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACgg                                                                                                                       >  2:5740745/1‑150 (MQ=255)

                                aGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACgg                                                                                                                       <  1:7884358/150‑1 (MQ=255)

                                 gggTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACggg                                                                                                                      >  2:4413221/1‑150 (MQ=255)

                                        aaGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCCCCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCg                                                                                                               >  2:2962864/1‑150 (MQ=255)

                                        aaGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCg                                                                                                               >  1:8313526/1‑150 (MQ=255)

                                        aaGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCg                                                                                                               >  2:8734498/1‑150 (MQ=255)

                                          gagGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTg                                                                                                             <  2:1122795/150‑1 (MQ=255)

                                          gagGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTg                                                                                                             <  1:6434251/150‑1 (MQ=255)

                                                ggTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTc                                                                                                       >  2:1664258/1‑150 (MQ=255)

                                                 gcgttcTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCGACGGTCAGGGCCGTACGGTCGATTTcc                                                                                                      <  2:3800178/145‑1 (MQ=255)

                                                 gcgtacTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCGACGGTCAGGGCCGTACGGTCGATTTcc                                                                                                      <  1:7571084/145‑1 (MQ=255)

                                                 gTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTcc                                                                                                      <  1:5915628/150‑1 (MQ=255)

                                                     ccTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCaa                                                                                                  <  2:357332/150‑1 (MQ=255)

                                                      cTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCGACGGTCAGGGCCGTACGGTCGATTTCCGCAac                                                                                                 >  2:4727921/1‑150 (MQ=255)

                                                        gACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAacac                                                                                               >  2:1578651/1‑150 (MQ=255)

                                                           cGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACcgt                                                                                            >  2:3303592/1‑150 (MQ=255)

                                                           cGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACcgt                                                                                            >  2:712285/1‑150 (MQ=255)

                                                            gAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACcgtg                                                                                           >  2:8196699/1‑150 (MQ=255)

                                                            gAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACcgtg                                                                                           >  1:2508804/1‑150 (MQ=255)

                                                                  gTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACcgtgatcgtg                                                                                     >  2:866860/1‑150 (MQ=255)

                                                                   tGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACcgtgatcgtga                                                                                    >  2:586315/1‑150 (MQ=255)

                                                                      ggcgCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGtgatga                                                                                 <  2:8671617/150‑1 (MQ=255)

                                                                      ggcgCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGtgatga                                                                                 >  1:311931/1‑150 (MQ=255)

                                                                       gcgcAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGAc                                                                                <  1:4020200/150‑1 (MQ=255)

                                                                        cgcAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGAcc                                                                               >  1:8068007/1‑150 (MQ=255)

                                                                          cAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTc                                                                             >  2:5554078/1‑150 (MQ=255)

                                                                           aaGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTcc                                                                            <  1:3804996/150‑1 (MQ=255)

                                                                             gCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCaa                                                                          >  1:7448634/1‑150 (MQ=255)

                                                                               catACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGATCAACCTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCAGCAACACCGTGATCGTGATGACCTCCAAcc                                                                        >  1:2884849/3‑150 (MQ=255)

                                                                                atatcaggtgatCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCGACGGTCAGGGCCGTACGGTCGATTTCCGCAACACGGTCATCATCATGACCTCGAACCt                                                                       >  2:1809796/7‑150 (MQ=255)

                                                                                 tatcaggtgatCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCGACGGTCAGGGCCGTACGGTCGATTTCCGCAACACGGTCATCATCATGACCTCGAACCtc                                                                      >  2:7838171/6‑149 (MQ=255)

                                                                                 tatcaggtgatCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCGACGGTCAGGGCCGTACGGTCGATTTCCGCAACACGGTCATCATCATGACCTCGAACCtc                                                                      >  1:7210281/6‑149 (MQ=255)

                                                                                    tCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGc                                                                   >  1:2938059/1‑150 (MQ=255)

                                                                                    tCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGc                                                                   >  2:2813385/1‑150 (MQ=255)

                                                                                      ggtggtGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGGTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCCCGGGCGCACCGTGGACTTCCGCAACACCGTGAGCGTGATGACCTCCAACCGGGGCTc                                                                 >  2:4599830/1‑150 (MQ=255)

                                                                                            gctgctGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCa                                                           >  1:7515242/1‑150 (MQ=255)

                                                                                              tgctgGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGgcg                                                             <  1:2648319/146‑1 (MQ=255)

                                                                                              tgctgGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGgcg                                                             >  2:2648319/1‑146 (MQ=255)

                                                                                              tgctgGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGa                                                         <  2:3633003/150‑1 (MQ=255)

                                                                                               gctgGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGAt                                                        >  2:1041657/1‑150 (MQ=255)

                                                                                                  ggACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCa                                                     <  2:1742086/150‑1 (MQ=255)

                                                                                                  ggACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCa                                                     <  2:4209830/150‑1 (MQ=255)

                                                                                                  ggACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCa                                                     >  1:5871121/1‑150 (MQ=255)

                                                                                                  ggACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAACCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCa                                                     <  1:1668960/150‑1 (MQ=255)

                                                                                                   gACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAg                                                    <  2:6708377/150‑1 (MQ=255)

                                                                                                   gACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAg                                                    <  1:495342/150‑1 (MQ=255)

                                                                                                   gACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAg                                                    <  1:6715528/150‑1 (MQ=255)

                                                                                                   gACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAg                                                    <  1:5323297/150‑1 (MQ=255)

                                                                                                      gAGGTGGAAAAAGTCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGaa                                                 >  2:6163931/1‑150 (MQ=255)

                                                                                                      gAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGaa                                                 <  1:4673659/150‑1 (MQ=255)

                                                                                                         gTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACtg                                              >  1:1553005/1‑150 (MQ=255)

                                                                                                          tGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACtgg                                             <  2:3499796/150‑1 (MQ=255)

                                                                                                          tGGAAAAAGCCCACCCGGATGTGTACAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACtgg                                             <  1:8990853/150‑1 (MQ=255)

                                                                                                           ggAAAAAGCCCACCCGGATGTGTTCAACGTGCTGGTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGAGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCAGGGCTCGGCGCAGATTCAGGAACtggt                                            >  1:1167579/1‑150 (MQ=255)

                                                                                                            gAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACtggtg                                           <  1:4413221/150‑1 (MQ=255)

                                                                                                             aaaaaGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACtggtgg                                          <  1:5621505/150‑1 (MQ=255)

                                                                                                             aaaaaGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACtggtgg                                          <  2:1897390/150‑1 (MQ=255)

                                                                                                               aaaGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTggtgggt                                        <  1:8596529/150‑1 (MQ=255)

                                                                                                                 aGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGa                                      <  2:8873738/150‑1 (MQ=255)

                                                                                                                 aGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGa                                      <  1:6637284/150‑1 (MQ=255)

                                                                                                                  gCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGAc                                     >  1:8431857/1‑150 (MQ=255)

                                                                                                                      aCCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACcgcg                                 >  2:3869934/1‑150 (MQ=255)

                                                                                                                        ccGGATGTGTTCATCGTGCTGTTGCAGGTGCTGTAAGACGGCCGCCTGACCGACAGCCACGGGCTCAACGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCTCAGATTCAGGAACTGGTGGGTGACCGCGAg                               <  1:5355103/150‑1 (MQ=255)

                                                                                                                          ggATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGc                             >  1:4468283/1‑150 (MQ=255)

                                                                                                                            aTGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGcac                           >  2:7758604/1‑150 (MQ=255)

                                                                                                                                 ttCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGACCTGGTGGGTGACCGCGAGGCACAGCGt                      >  2:9002037/1‑150 (MQ=255)

                                                                                                                                 ttCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGt                      >  2:7302848/1‑150 (MQ=255)

                                                                                                                                 ttCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGt                      >  2:6676684/1‑150 (MQ=255)

                                                                                                                                  tCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTg                     >  2:2013095/1‑150 (MQ=255)

                                                                                                                                   cAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAgg                              >  2:6124058/1‑140 (MQ=255)

                                                                                                                                   cAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGc                    <  1:2298003/150‑1 (MQ=255)

                                                                                                                                    aaCGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCg                   >  2:1415776/1‑150 (MQ=255)

                                                                                                                                      cGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGc                 <  2:2508804/150‑1 (MQ=255)

                                                                                                                                        tGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGCAg               <  2:7397733/150‑1 (MQ=255)

                                                                                                                                           tGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGCAGTga            <  1:1409313/150‑1 (MQ=255)

                                                                                                                                            gTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGCAGTgat           <  1:425405/150‑1 (MQ=255)

                                                                                                                                            gTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCAGCAGTgat           >  1:1495621/1‑150 (MQ=255)

                                                                                                                                               gCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGCAGTgatgga        >  2:8449569/1‑150 (MQ=255)

                                                                                                                                               gCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGCAGTgatgga        <  2:212283/150‑1 (MQ=255)

                                                                                                                                                cAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGCAGTgatggat       <  1:1813896/150‑1 (MQ=255)

                                                                                                                                                     gCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGCAGTGATGGATGCGGt  >  2:6777372/1‑150 (MQ=255)

GCGCGCCTGATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGCAGTGATGGATGCGGT  >  NC_002947/732393‑732691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`