BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	732,557	C→T	8.3%	R698R (CGC→CGT)	clpB →	chaperone protein ClpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	732,557	0	C	T	8.3%	792.0 / 41.8	266	R698R (CGC→CGT)	clpB	chaperone protein ClpB
Reads supporting (aligned to +/- strand): ref base C (146/97); new base T (15/7); total (162/104)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 276 total aligned reads displayed.

GCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGCAGTGATGGATGCGGTGGGGGCGCACTTCCG  >  NC_002947/732408‑732706

  accaccAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCt                                                                                                                                                      >  2:5016700/1‑150 (MQ=255)

  accaccAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCt                                                                                                                                                      >  2:182672/1‑150 (MQ=255)

     tcccggttATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGAc                                                                                                                                                   >  2:1721377/8‑150 (MQ=255)

        aGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGa                                                                                                                                                <  1:8373731/150‑1 (MQ=255)

         ggCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGAc                                                                                                                                               >  1:1260647/1‑150 (MQ=255)

              tGTAGGGTATGAAGAGGCCGGTTACCTGACCTATGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGACGTGGAAAAAGCCCACCCGGATGAGTTCAACGTGCTGTTGCAGGTGCTGTAAGACGGCAGCCTGACCGACAGccc                                                                                                                                          >  2:5355103/1‑149 (MQ=255)

                tAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACg                                                                                                                                        >  2:8823311/1‑150 (MQ=255)

                 aGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACgg                                                                                                                                       >  2:5740745/1‑150 (MQ=255)

                  gggTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACgg‑g                                                                                                                                     >  2:4413221/1‑150 (MQ=255)

                  ggctATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGAc                                                                                                                                                   <  1:7825273/134‑1 (MQ=255)

                   ggTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑gc                                                                                                                                    <  1:5527061/150‑1 (MQ=255)

                    gTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACCGCCACGG‑gcg                                                                                                                                   >  2:3900902/1‑150 (MQ=255)

                       tGAAGAGGTCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCAc                                                                                                                                >  1:8197970/1‑150 (MQ=255)

                       tGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCAc                                                                                                                                >  1:1728502/1‑150 (MQ=255)

                        gAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCAcc                                                                                                                               >  1:2910125/1‑150 (MQ=255)

                        gAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCAcc                                                                                                                               >  1:3048433/1‑150 (MQ=255)

                         aaGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCg                                                                                                                              >  2:4671342/1‑150 (MQ=255)

                           gagGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTg                                                                                                                            >  1:3684047/1‑150 (MQ=255)

                           gagGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTg                                                                                                                            <  2:1122795/150‑1 (MQ=255)

                           gagGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTg                                                                                                                            >  1:4707677/1‑150 (MQ=255)

                           gagGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTg                                                                                                                            <  1:6434251/150‑1 (MQ=255)

                            agGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGG‑AAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGTGCGC‑CCGTggc                                                                                                                          <  1:1520500/150‑2 (MQ=255)

                             gggCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGa                                                                                                                          <  2:360506/150‑1 (MQ=255)

                              ggCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGACAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGTGCGC‑CCGTggct                                                                                                                         <  2:5646240/150‑3 (MQ=255)

                              ggCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGAc                                                                                                                         >  1:5861312/1‑150 (MQ=255)

                                 ggTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTc                                                                                                                      >  2:1664258/1‑150 (MQ=255)

                                  gcgttcTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCGACGGTCAGGG‑CCGTACGGTCGATTTcc                                                                                                                     <  2:3800141/145‑1 (MQ=255)

                                  gTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTcc                                                                                                                     <  1:5915628/150‑1 (MQ=255)

                                    tACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGc                                                                                                                   <  1:4420156/150‑1 (MQ=255)

                                      ccTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCaa                                                                                                                 <  2:357323/150‑1 (MQ=255)

                                        tGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCGACGGTCAGGG‑CCGTACGGTCGATTTCCGCAaca                                                                                                               <  2:4553908/150‑1 (MQ=255)

                                        tGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAaca                                                                                                               <  2:6724865/150‑1 (MQ=255)

                                          aCCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACAcc                                                                                                             >  1:2244064/1‑150 (MQ=255)

                                            cGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACcgt                                                                                                           >  2:712285/1‑150 (MQ=255)

                                             gAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACcgtg                                                                                                          >  1:2508804/1‑150 (MQ=255)

                                                   gTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACcgtgatcgtg                                                                                                    <  1:4135697/150‑1 (MQ=255)

                                                    tGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCTGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACcgtgatcgtga                                                                                                   <  1:4978725/150‑1 (MQ=255)

                                                    tGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACcgtgatcgtga                                                                                                   >  2:586315/1‑150 (MQ=255)

                                                    tGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACcgtgatcgtga                                                                                                   <  2:4829005/150‑1 (MQ=255)

                                                       ggcgCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGtgatga                                                                                                <  2:8671617/150‑1 (MQ=255)

                                                        gcgcAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGAc                                                                                               <  1:4020200/150‑1 (MQ=255)

                                                          gcAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCt                                                                                             >  1:5299426/1‑150 (MQ=255)

                                                          gcAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCt                                                                                             >  1:3378306/1‑150 (MQ=255)

                                                                cTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAAcc                                                                                       >  2:2760747/1‑150 (MQ=255)

                                                                cTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAAcc                                                                                       >  1:9105266/1‑150 (MQ=255)

                                                                  tatcaggtgatCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCGACGGTCAGGG‑CCGTACGGTCGATTTCCGCAACACGGTCATCATCATGACCTCGAACCtc                                                                                     >  2:7838171/6‑149 (MQ=255)

                                                                  tatcaggtgatCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGACCGACGGTCAGGG‑CCGTACGGTCGATTTCCGCAACACGGTCATCATCATGACCTCGAACCtc                                                                                     >  1:7210281/6‑149 (MQ=255)

                                                                   aCTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTgg                                                                                    <  2:2910125/150‑1 (MQ=255)

                                                                       ggtggtGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTc                                                                                >  2:5087768/1‑150 (MQ=255)

                                                                       ggtggtGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTc                                                                                >  2:8322058/1‑150 (MQ=255)

                                                                       ggtggtGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTc                                                                                >  2:7897116/1‑150 (MQ=255)

                                                                       ggtggtGCTGCTGGACGACCTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCCCCGTGGACTTCCGCACCACCGTGATCGTGATGACCTCCAACCTGGGcgc                                                                                >  2:6136072/1‑148 (MQ=255)

                                                                             gctgctGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCa                                                                          <  1:7466987/150‑1 (MQ=255)

                                                                               tgctgGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGgcg                                                                            <  1:2648319/146‑1 (MQ=255)

                                                                               tgctgGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGa                                                                        >  1:1934273/1‑150 (MQ=255)

                                                                                gctgGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGAt                                                                       >  1:2485306/1‑150 (MQ=255)

                                                                                   ggACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCa                                                                    <  2:6304960/150‑1 (MQ=255)

                                                                                   ggACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCa                                                                    <  2:4209830/150‑1 (MQ=255)

                                                                                   ggACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCa                                                                    <  1:7518381/150‑1 (MQ=255)

                                                                                   ggACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAACCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCa                                                                    <  1:1668960/150‑1 (MQ=255)

                                                                                    gACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAg                                                                   <  1:6715528/150‑1 (MQ=255)

                                                                                       gAGGTGGAAAAAGTCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGaa                                                                >  2:1560794/1‑150 (MQ=255)

                                                                                       gAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGaa                                                                <  1:4673659/150‑1 (MQ=255)

                                                                                          gTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACtg                                                             >  1:1432047/1‑150 (MQ=255)

                                                                                           tGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACtgg                                                            <  2:3499686/150‑1 (MQ=255)

                                                                                            ggAAAAAGCCCACCCGGATGTGTTCAACGTGCTGGTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGAGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCAGGGCTCGGCGCAGATTCAGGAACtggt                                                           >  1:1167579/1‑150 (MQ=255)

                                                                                             gAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACtggtg                                                          <  1:4413209/150‑1 (MQ=255)

                                                                                                ataGCCTACGCGGATGTGTTCATCGTGCTGTTGCAGGTGCTGTTAGACGGCCGCCTTTAGGACTGCCTCGG‑GCGCTGCTTGGTGTTCGGCTACACCGTGATCGTGATGACCTCCTACCTGGGCTCGGCGCAGATTCAGGTAGTggtgggt                                                       <  2:8722537/148‑1 (MQ=255)

                                                                                                aaaGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTggtgggt                                                       <  1:8596529/150‑1 (MQ=255)

                                                                                                 aaGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTggtgggtg                                                      >  1:8843757/1‑150 (MQ=255)

                                                                                                  aGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGa                                                     <  2:8873738/150‑1 (MQ=255)

                                                                                                   gCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGAc                                                    >  1:6559361/1‑150 (MQ=255)

                                                                                                   gCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGAc                                                    >  1:8431857/1‑150 (MQ=255)

                                                                                                     ccACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACcg                                                  >  2:6702649/1‑150 (MQ=255)

                                                                                                        cccGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGa                                               <  2:8596929/150‑1 (MQ=255)

                                                                                                         ccGGATGTGTTCATCGTGCTGTTGCAGGTGCTGTAAGACGGCCGCCTGACCGACAGCCACGG‑GCTCAACGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCTCAGATTCAGGAACTGGTGGGTGACCGCGAg                                              <  1:5355103/150‑1 (MQ=255)

                                                                                                         ccGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAg                                              >  2:2083116/1‑150 (MQ=255)

                                                                                                             aTGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGcac                                          >  2:7758604/1‑150 (MQ=255)

                                                                                                                  ttCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGACCTGGTGGGTGACCGCGAGGCACAGCGt                                     >  2:9002037/1‑150 (MQ=255)

                                                                                                                  ttCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGt                                     >  2:7302848/1‑150 (MQ=255)

                                                                                                                   tCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTg                                    >  2:2013095/1‑150 (MQ=255)

                                                                                                                    cAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAgg                                             <  1:6124058/140‑1 (MQ=255)

                                                                                                                     aaCGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCg                                  >  2:3102400/1‑150 (MQ=255)

                                                                                                                     aaCGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCg                                  >  2:1415776/1‑150 (MQ=255)

                                                                                                                       cGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGACCTGGTGGGTGACCGCGAGGCACAGCGTGCGGc                                >  1:8078138/1‑150 (MQ=255)

                                                                                                                       cGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGc                                <  2:2508804/150‑1 (MQ=255)

                                                                                                                         tGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGCAg                              <  2:7397733/150‑1 (MQ=255)

                                                                                                                                 cAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGCAGTgatggat                      >  1:5068832/1‑150 (MQ=255)

                                                                                                                                      gCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGCAGTGATGGATGCGGt                 >  2:6777372/1‑150 (MQ=255)

                                                                                                                                          gAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGCAGTGATGGATGCGGTgggg             <  2:1786265/150‑1 (MQ=255)

                                                                                                                                                ggCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGCAGTGATGGATGCGGTGGGGGCGCAc       >  2:7708199/1‑150 (MQ=255)

                                                                                                                                                ggCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGCAGTGATGGATGCGGTGGGGGCGCAc       >  1:7931950/1‑150 (MQ=255)

                                                                                                                                                ggCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGCAGTGATGGATGCGGTGGGGGCGCAc       >  1:7325546/1‑150 (MQ=255)

                                                                                                                                                ggCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGCAGTGATGGATGCGGTGGGGGCGCAc       >  2:2667882/1‑150 (MQ=255)

                                                                                                                                                ggCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGCAGTGATGGATGCGGTGGGGGCGCAc       >  2:3401123/1‑150 (MQ=255)

                                                                                                                                                ggCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGCAGTGATGGATGCGGTGGGGGCGCAc       >  1:8759315/1‑150 (MQ=255)

                                                                                                                                                ggCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTACGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGCAGTGATGGATGCGGTGGGGGCGCAc       >  2:220750/1‑150 (MQ=255)

                                                                                                                                                 gccgccTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGaa                                                                <  2:3850674/92‑1 (MQ=255)

                                                                                                                                                 gccgccTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGaa                                                                >  1:3850674/1‑92 (MQ=255)

                                                                                                                                                 gccgccTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGCAGTGATGGATGCGGTGGGGGCGCACt      >  2:4403519/1‑150 (MQ=255)

GCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGG‑GCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGCAGTGATGGATGCGGTGGGGGCGCACTTCCG  >  NC_002947/732408‑732706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`