BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	733,648	A→T	6.5%	intergenic (+19/‑28)	PP_t09 → / → PP_t10	tRNA‑Lys/tRNA‑Pro

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	733,648	0	A	T	6.5%	571.7 / 17.4	193	intergenic (+19/‑28)	PP_t09/PP_t10	tRNA‑Lys/tRNA‑Pro
Reads supporting (aligned to +/- strand): ref base A (103/71); new base T (2/12); total (106/87)
Fisher's exact test for biased strand distribution p-value = 1.41e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 205 total aligned reads displayed.

CTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACC  >  NC_002947/733499‑733797

cTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCa                                                                                                                                                        >  1:7616307/1‑150 (MQ=255)

  gCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCaaa                                                                                                                                                      <  2:8015293/150‑1 (MQ=255)

  gCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCaaa                                                                                                                                                      <  1:2677089/150‑1 (MQ=255)

  gCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCaaa                                                                                                                                                      <  1:928107/150‑1 (MQ=255)

    tttGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAaga                                                                                                                                                    <  2:6120734/150‑1 (MQ=255)

    tttGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAaga                                                                                                                                                    <  1:86021/150‑1 (MQ=255)

      tGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAgaga                                                                                                                                                  <  1:4567899/150‑1 (MQ=255)

        ggAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATg                                                                                                                                                <  1:1466320/150‑1 (MQ=255)

           gCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCTTTGAGATGccc                                                                                                                                             <  1:8148890/150‑1 (MQ=255)

           gCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCTTTGAGATGccc                                                                                                                                             <  1:3328356/150‑1 (MQ=255)

            cAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑t                                                                                                                                           <  2:5410278/150‑1 (MQ=255)

             aGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCTTTGAGATGCCC‑tt                                                                                                                                          <  2:7892893/150‑1 (MQ=255)

                aTGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAAAAAAGGGCATCTCTTTGAGATGCCC‑ttttc                                                                                                                                       <  2:295166/150‑2 (MQ=255)

                  gTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTCACCAATTGGTCGTGGGTTCGAATCCGACACGACCCACCAGGTAGAAAAGGGCAGCTCAAAGAGATGCCC‑ttttttt                                                                                                                                     >  2:8451319/1‑150 (MQ=17)

                  gTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCTTTGAGATGCCC‑ttttcta                                                                                                                                     <  2:3413934/150‑4 (MQ=255)

                  gTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑ttttttt                                                                                                                                     <  1:2871449/150‑1 (MQ=255)

                     aGGAGTTCGAATCCCCTCACCCCGACCTTTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAACGAGATGCCC‑TTTTTTTTGa                                                                                                                                  <  2:5936039/150‑1 (MQ=255)

                       gAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCAGGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGaaa                                                                                                                                >  1:8330535/1‑150 (MQ=255)

                       gAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTGTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑GTTTTTTTGaaa                                                                                                                                >  2:6772932/1‑150 (MQ=255)

                          ttCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAATTGGGCATCTCAATTAGTTGGGG‑TTTTTTTTgtttgtg                                                                                                                             >  2:4852000/1‑144 (MQ=14)

                          ttCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAAGAAAGGGGCATCTCAAGGACAAGCCC‑GTTTATTAGAAacac                                                                                                                             >  1:5692395/1‑150 (MQ=14)

                            cGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTc                                                                                                                           <  1:1268018/150‑1 (MQ=255)

                             gAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTATAAAAGGGCAACACAAAGAGAGGCCC‑TTATATTTGAAACACTCg                                                                                                                          >  1:542653/1‑150 (MQ=18)

                             gAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCg                                                                                                                          <  2:8206867/150‑1 (MQ=255)

                             gAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCg                                                                                                                          <  1:6578651/150‑1 (MQ=255)

                             gAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACACg                                                                                                                          >  1:1068073/1‑150 (MQ=255)

                              aaTCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCgg                                                                                                                         <  1:1876687/150‑1 (MQ=255)

                                  cccTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGgtg                                                                                                                     >  1:7468501/1‑150 (MQ=255)

                                   ccTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGgtgt                                                                                                                    >  2:1539525/1‑150 (MQ=255)

                                   ccTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TATTTTTTGAAACACTAGGGgtgt                                                                                                                    >  1:5525415/1‑150 (MQ=255)

                                     tCACCCCGACCATATTCCGGGTCGTTAGCTCAGTTGGTAGACCAGTTGGCATTTAACCAATTGGACGTAGGATCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑AAATTTTTGAAACACTCGGGGTGTAg                                                                                                                  <  2:1338195/150‑1 (MQ=21)

                                      cACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATg                                                                                                                                                <  1:6149360/120‑1 (MQ=255)

                                      cACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATg                                                                                                                                                >  2:6149360/1‑120 (MQ=255)

                                       aCCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAgcg                                                                                                                >  1:3520419/1‑150 (MQ=255)

                                       aCCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGCAgcg                                                                                                                >  1:639642/1‑150 (MQ=255)

                                         cccGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCa                                                                                                              <  2:4340705/150‑1 (MQ=255)

                                         cccGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACAATCGGGGTGTAgcgcc                                                                                                              >  2:7513985/1‑149 (MQ=255)

                                         cccGACCATTTTCCGGGTCGTGAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCa                                                                                                              <  2:5506412/150‑1 (MQ=255)

                                         cccGACCATTTACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCa                                                                                                              >  1:4364090/1‑150 (MQ=255)

                                          ccGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACGCGGGGTGTAGCGCAg                                                                                                             >  1:1260040/1‑150 (MQ=255)

                                           cGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTGTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGt                                                                                                            >  1:1171662/1‑150 (MQ=255)

                                            gACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAATGGGAATATCAAAGAGATGGCC‑TTTATTTTGAAACACTCGGGGTGTAGCGCAGTc                                                                                                           >  2:4460131/1‑150 (MQ=21)

                                               cATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACGATTTGGTCGTAGGTTCGAATCCTACACGACCCACCCTGTAGAAAAGGGCAACTCAAAGAGATGCAC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCgg                                                                                                        <  1:4603158/150‑1 (MQ=25)

                                                aTTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACAATCGGGGTGTAGCGCAGTCCGGt                                                                                                       >  2:2456714/1‑150 (MQ=255)

                                                 ttttCCGGGTCGTTAGATCAGTTGGTAGATCAGTTGTCTTTTAACCAATTGGTCGTATTTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTa                                                                                                      <  1:6615376/150‑1 (MQ=25)

                                                  tttCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAg                                                                                                     <  2:3986886/150‑1 (MQ=255)

                                                         gTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCt                                                                                              >  2:882167/1‑150 (MQ=255)

                                                             ttAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCtt                                                                                          >  2:5460255/1‑150 (MQ=255)

                                                              tAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAACGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCttt                                                                                         <  1:3002570/150‑1 (MQ=255)

                                                               aGCTCAGTTGGTAGAGCAGTTGGCTTTTCACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCCTGTAGAAAAGGGCCTCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTg                                                                                        <  2:646574/150‑1 (MQ=255)

                                                                gCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTTCACGACCCACCATGTAGAAAAGGGCACCTCAATGAGATGCAC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTgg                                                                                       <  2:4701218/150‑1 (MQ=255)

                                                                gCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTgg                                                                                       <  1:7901610/150‑1 (MQ=255)

                                                                gCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGATCTAATCCTACACGACCCAACATCAAGAAAAGCGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTgg                                                                                       >  2:8638449/1‑150 (MQ=25)

                                                                  tCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGa                                                                                     >  2:4587283/1‑150 (MQ=255)

                                                                  tCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGa                                                                                     >  1:1548196/1‑150 (MQ=255)

                                                                  gcAGTTGGGAGAGCAGTTGGCTTTGACCCCCTGGGGCGTAGGTTCGAAGCCTCCACGCCCCACCCTGTAGAAAAGGGCAGCTCACAGAGAGGCCG‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGa                                                                                     <  2:75775/149‑1 (MQ=25)

                                                                    tgTTTGTTGAGGAGTTTGCTTTTAACCTATTGGTCGTAGGTTCGTATCCTACACGACCCACAATGTAGAAATGGGCATCTCTAAGTGATGCAC‑TTTTTTTTGAAACTCTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGc                                                                                   <  1:477007/149‑1 (MQ=37)

                                                                                 gTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCaaa                                                                                                                                                      <  2:2198899/71‑1 (MQ=255)

                                                                                    ggCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTgg                                                                                       <  1:4520070/130‑1 (MQ=255)

                                                                                    ggCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTgg                                                                                       >  2:4519979/1‑130 (MQ=255)

                                                                                    ggCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTgg                                                                                       >  2:4520070/1‑130 (MQ=255)

                                                                                    ggCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTgg                                                                                       <  1:4519979/130‑1 (MQ=255)

                                                                                       ttttAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGa                                                                 >  1:3073964/1‑150 (MQ=255)

                                                                                           aaCCAATTGGTCGTATGTTCGATTCCTACACGACCCACCCTCTAGATATGTGCATCTGCATGCCTTGCAG‑TTATTTTTGCCACTCTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATccc                                                            <  2:1830236/150‑1 (MQ=17)

                                                                                           aaCCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATccc                                                            <  1:4831478/150‑1 (MQ=255)

                                                                                           aaCCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGAATCTCAAAGAGATGCCC‑TTTTTTTTGAAACAATCGGGGTGTTGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATccc                                                            >  1:2358688/1‑150 (MQ=255)

                                                                                            aCCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATcccc                                                           >  2:6524696/1‑150 (MQ=255)

                                                                                            aCCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATccca                                                           >  1:652193/1‑149 (MQ=255)

                                                                                                             tCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCAttttt                                          >  1:8677373/1‑150 (MQ=255)

                                                                                                               gAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCttt                                                                                         >  1:5203963/1‑101 (MQ=255)

                                                                                                               gAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTgg                                        <  1:6772932/150‑1 (MQ=255)

                                                                                                               gAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCAGTTTTgg                                        >  2:5230844/1‑150 (MQ=255)

                                                                                                                aaTCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAg                                                                                 >  2:8479844/1‑108 (MQ=255)

                                                                                                                aaTCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTggg                                       >  1:2365468/1‑150 (MQ=255)

                                                                                                                aaTCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTGTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTggg                                       >  2:3858746/1‑150 (MQ=255)

                                                                                                                    cTACACGACCCTCCATGTAGAAAATTGCCCCTCCAAGAGATGCAC‑TTTTTTTTGACACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGt                                   <  1:3087727/150‑1 (MQ=18)

                                                                                                                    cTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGt                                   >  1:6254101/1‑150 (MQ=255)

                                                                                                                     tACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGtt                                  >  1:6468960/1‑150 (MQ=255)

                                                                                                                     tACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGAAGTCAGGAGTTCGACTCCCCTCACCCCGACCATTTTTGGGTCGtt                                  >  1:5863719/1‑150 (MQ=255)

                                                                                                                      acacGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTa                                 <  2:1081138/150‑1 (MQ=255)

                                                                                                                      acacGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGACC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCTCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTa                                 <  1:8451319/150‑1 (MQ=255)

                                                                                                                         cGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCCGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCAGTTTTGGGTCGTTAGCt                              >  2:5704470/1‑150 (MQ=255)

                                                                                                                         cGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCt                              >  2:8973979/1‑150 (MQ=255)

                                                                                                                           aCCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCa                            >  2:2360099/1‑150 (MQ=255)

                                                                                                                            cccACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTa                                                                                                      <  2:3624560/75‑1 (MQ=255)

                                                                                                                              caccaTGTATAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGtt                         <  1:3017823/150‑1 (MQ=255)

                                                                                                                              caccaTGTAGAAACGGGCATCACACACAGATGCCC‑TATTTTTTGAAACTCTCGGGGTGTAGCGCAGTCCTGTAGAGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGtt                         <  2:4989731/150‑1 (MQ=18)

                                                                                                                               accaTGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTGTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTg                        >  2:5961626/1‑150 (MQ=255)

                                                                                                                                 ttcaaaaaaaaGGGCATCTCTTTGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGt                      >  2:7794784/8‑150 (MQ=18)

                                                                                                                                 caTGTAGAAAATGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTAGGGGTGTAGCGCAGTCCGGTAGCGCGCCTTCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGATCAGATGGt                      >  2:2102725/1‑150 (MQ=21)

                                                                                                                                  aTGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGGCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTa                     >  1:599018/1‑150 (MQ=255)

                                                                                                                                          aaGGGCATCTCAACGAGACGCCC‑AATTTTTTGATTCCCTCGGCGTGTCGCGCAGTCAGGTTGCCCGCCTGCTCCGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGtt             <  2:708486/150‑1 (MQ=17)

                                                                                                                                           aGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTg            >  2:4049457/1‑150 (MQ=255)

                                                                                                                                            gggCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTCGCGCGCCTGCTGTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTgg           >  1:5406631/1‑150 (MQ=255)

                                                                                                                                               cATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCtt        <  2:4828261/150‑1 (MQ=255)

                                                                                                                                               cATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCtt        >  1:8928909/1‑150 (MQ=255)

                                                                                                                                                  ctcAACGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTa     <  1:7827860/150‑1 (MQ=255)

                                                                                                                                                   tcAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTaa    >  1:6315204/1‑150 (MQ=255)

                                                                                                                                                     aaaGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAAcc  >  2:5371621/1‑150 (MQ=255)

                                                                                                                                                     aaaGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTACCGCAGACCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGATGGCTTTTAAca  >  2:7955261/1‑149 (MQ=255)

CTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACC  >  NC_002947/733499‑733797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`