BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	733,650	A→T	6.9%	intergenic (+21/‑26)	PP_t09 → / → PP_t10	tRNA‑Lys/tRNA‑Pro

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	733,650	0	A	T	6.9%	532.2 / 17.9	189	intergenic (+21/‑26)	PP_t09/PP_t10	tRNA‑Lys/tRNA‑Pro
Reads supporting (aligned to +/- strand): ref base A (100/69); new base T (3/11); total (105/84)
Fisher's exact test for biased strand distribution p-value = 9.61e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 205 total aligned reads displayed.

GCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAA  >  NC_002947/733501‑733799

gCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCaaa                                                                                                                                                         <  1:928107/150‑1 (MQ=255)

gCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCaaa                                                                                                                                                         <  2:8015293/150‑1 (MQ=255)

  tttGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAaga                                                                                                                                                       <  1:86021/150‑1 (MQ=255)

  tttGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAaga                                                                                                                                                       <  2:6120734/150‑1 (MQ=255)

      ggAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATg                                                                                                                                                   <  1:1466320/150‑1 (MQ=255)

       gAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGc                                                                                                                                                  <  1:7391909/150‑1 (MQ=255)

         gCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCTTTGAGATGccc                                                                                                                                                <  1:8148890/150‑1 (MQ=255)

          cAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑t                                                                                                                                              <  2:5410278/150‑1 (MQ=255)

           cggATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑tt                                                                                                                                             <  1:3192697/149‑1 (MQ=255)

           aGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCTTTGAGATGCCC‑tt                                                                                                                                             <  2:7892893/150‑1 (MQ=255)

              aTGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAAAAAAGGGCATCTCTTTGAGATGCCC‑ttttc                                                                                                                                          <  2:295166/150‑2 (MQ=255)

                gTCAGGTGTTCGATTCCCCTCACCCCGACCTTTTTCCGGGTCGTTTGCTCAGTTGGTTGTGCTGTTGGCTTTTAACCAATTGGTCGTAGGTTCGATTGCTACACGACCCACCATGTAGTATAGGGCATCTCAAAGAGATGCCC‑ttttttt                                                                                                                                        <  1:2871545/150‑1 (MQ=17)

                gTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTCACCAATTGGTCGTGGGTTCGAATCCGACACGACCCACCAGGTAGAAAAGGGCAGCTCAAAGAGATGCCC‑ttttttt                                                                                                                                        >  2:8451319/1‑150 (MQ=17)

                gTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCTTTGAGATGCCC‑ttttcta                                                                                                                                        <  2:3413934/150‑4 (MQ=255)

                 tCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑tttttttt                                                                                                                                       <  1:6426372/150‑1 (MQ=255)

                   aGGAGTTCGAATCCCCTCACCCCGACCTTTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAACGAGATGCCC‑TTTTTTTT‑Ga                                                                                                                                    <  2:5936039/150‑1 (MQ=255)

                     gAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCAGGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑Gaaa                                                                                                                                  >  1:8330535/1‑150 (MQ=255)

                     gAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTGTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑GTTTTTTT‑Gaaa                                                                                                                                  >  2:6772932/1‑150 (MQ=255)

                       gTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAaca                                                                                                                                >  1:3447977/1‑150 (MQ=255)

                        ttCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAATTGGGCATCTCAATTAGTTGGGG‑TTTTTTTT‑gtttgtg                                                                                                                               >  2:4852000/1‑144 (MQ=14)

                          cGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTT‑GAAACACt                                                                                                                              <  1:4581157/150‑1 (MQ=255)

                          cGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTc                                                                                                                             <  1:1268018/150‑1 (MQ=255)

                           gAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCg                                                                                                                            <  2:8206867/150‑1 (MQ=255)

                           gAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACACg                                                                                                                            >  1:1068073/1‑150 (MQ=255)

                            aaTCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCgg                                                                                                                           <  1:657594/150‑1 (MQ=255)

                            aaTCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCgg                                                                                                                           <  1:1876687/150‑1 (MQ=255)

                             aTCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑TAAACACTCggg                                                                                                                          >  1:3003234/1‑150 (MQ=255)

                              tCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCgggg                                                                                                                         <  1:4553474/150‑1 (MQ=255)

                                 ccTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGgtgt                                                                                                                      >  2:1539525/1‑150 (MQ=255)

                                   tCACCCCGACCATATTCCGGGTCGTTAGCTCAGTTGGTAGACCAGTTGGCATTTAACCAATTGGACGTAGGATCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑AAATTTTT‑GAAACACTCGGGGTGTAg                                                                                                                    <  2:1338195/150‑1 (MQ=21)

                                    cACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATg                                                                                                                                                   >  2:6149360/1‑120 (MQ=255)

                                       cccGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCa                                                                                                                <  2:4340705/150‑1 (MQ=255)

                                       cccGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACAATCGGGGTGTAgcgcc                                                                                                                >  2:7513985/1‑149 (MQ=255)

                                       cccGACCATTTTCCGGGTCGTGAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCa                                                                                                                <  2:5506412/150‑1 (MQ=255)

                                        ccGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACGCGGGGTGTAGCGCAg                                                                                                               >  1:1260040/1‑150 (MQ=255)

                                         cGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTGTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGt                                                                                                              >  1:1171662/1‑150 (MQ=255)

                                          gACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAATGGGAATATCAAAGAGATGGCC‑TTTATTTT‑GAAACACTCGGGGTGTAGCGCAGTc                                                                                                             >  2:4460131/1‑150 (MQ=21)

                                              aTTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACAATCGGGGTGTAGCGCAGTCCGGt                                                                                                         >  2:2456714/1‑150 (MQ=255)

                                                tttCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAg                                                                                                       <  2:3986886/150‑1 (MQ=255)

                                                       gTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCt                                                                                                >  2:882167/1‑150 (MQ=255)

                                                           ttAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCtt                                                                                            >  2:5460255/1‑150 (MQ=255)

                                                             aGCTCAGTTGGTAGAGCTGTTGGCTTTTAACCTATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTg                                                                                          <  1:2732137/150‑1 (MQ=255)

                                                             aGCTCAGTTGGTAGAGCAGTTGGCTTTTCACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCCTGTAGAAAAGGGCCTCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTg                                                                                          <  2:646574/150‑1 (MQ=255)

                                                              gCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTTCACGACCCACCATGTAGAAAAGGGCACCTCAATGAGATGCAC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTgg                                                                                         <  2:4701218/150‑1 (MQ=255)

                                                              gCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTgg                                                                                         <  1:7901610/150‑1 (MQ=255)

                                                              gCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGATCTAATCCTACACGACCCAACATCAAGAAAAGCGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTgg                                                                                         >  2:8638449/1‑150 (MQ=25)

                                                                tCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGa                                                                                       >  2:4587283/1‑150 (MQ=255)

                                                                tCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGa                                                                                       >  1:1548196/1‑150 (MQ=255)

                                                                gcAGTTGGGAGAGCAGTTGGCTTTGACCCCCTGGGGCGTAGGTTCGAAGCCTCCACGCCCCACCCTGTAGAAAAGGGCAGCTCACAGAGAGGCCG‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGa                                                                                       <  2:75775/149‑1 (MQ=25)

                                                                   gTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCa                                                                                    <  1:7593162/150‑1 (MQ=255)

                                                                          gagCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTc                                                                             >  1:263217/1‑150 (MQ=255)

                                                                           agCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCa                                                                            >  1:5965986/1‑150 (MQ=255)

                                                                               gTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCaaa                                                                                                                                                         >  1:2198899/1‑71 (MQ=255)

                                                                               gTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCaaa                                                                                                                                                         <  2:2198899/71‑1 (MQ=255)

                                                                                  ggCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTgg                                                                                         >  2:4519979/1‑130 (MQ=255)

                                                                                  ggCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTgg                                                                                         >  2:4520070/1‑130 (MQ=255)

                                                                                  ggCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTgg                                                                                         <  1:4520062/130‑1 (MQ=255)

                                                                                     ttttAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAGGGGAAACTCAAAGAGATCCCCATATTGTTA‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGa                                                                   >  1:3074357/1‑150 (MQ=25)

                                                                                         aaCCAATTGGTCGTATGTTCGATTCCTACACGACCCACCCTCTAGATATGTGCATCTGCATGCCTTGCAG‑TTATTTTT‑GCCACTCTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATccc                                                              <  2:1830236/150‑1 (MQ=17)

                                                                                          aCCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATcccc                                                             >  1:5459588/1‑150 (MQ=255)

                                                                                          aCCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATcccc                                                             >  2:6524696/1‑150 (MQ=255)

                                                                                                 ggTCGTAGGTTCGAATCCTACACGACCCACCATCTAGAAAAGGGCATCTCAAACAGATGCCC‑ATTATTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCAcccc                                                      <  1:6682373/150‑1 (MQ=255)

                                                                                                     gTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGAcc                                                  >  1:3036128/1‑150 (MQ=255)

                                                                                                      tAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCa                                                 >  1:6584877/1‑150 (MQ=255)

                                                                                                          ttCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCAtttt                                             <  1:4704811/150‑1 (MQ=255)

                                                                                                           tCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCAttttt                                            >  1:8677373/1‑150 (MQ=255)

                                                                                                           tCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCAttttt                                            >  1:627121/1‑150 (MQ=255)

                                                                                                             gAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCAGTTTTgg                                          >  2:5230844/1‑150 (MQ=255)

                                                                                                              aaTCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAg                                                                                   >  2:8479844/1‑108 (MQ=255)

                                                                                                              aaTCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTggg                                         >  1:6043743/1‑150 (MQ=255)

                                                                                                              aaTCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTggg                                         >  1:6508713/1‑150 (MQ=255)

                                                                                                              aaTCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTggg                                         >  1:4460344/1‑150 (MQ=255)

                                                                                                              aaTCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTGTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTggg                                         >  2:3858746/1‑150 (MQ=255)

                                                                                                                   tACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGtt                                    >  1:5862947/1‑150 (MQ=255)

                                                                                                                    acacGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTa                                   <  2:1081138/150‑1 (MQ=255)

                                                                                                                    acacGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGACC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCTCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTa                                   <  1:8451319/150‑1 (MQ=255)

                                                                                                                      acGACCCACCCTGTATAATAGGGCATCTCCAAGTGATGCCC‑TTATTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGc                                 <  1:5170636/150‑1 (MQ=18)

                                                                                                                       cGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCCGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCAGTTTTGGGTCGTTAGCt                                >  2:5704470/1‑150 (MQ=255)

                                                                                                                       cGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCt                                >  2:8973979/1‑150 (MQ=255)

                                                                                                                         aCCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCa                              >  2:2360099/1‑150 (MQ=255)

                                                                                                                          cccACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTa                                                                                                        >  1:3624560/1‑75 (MQ=255)

                                                                                                                          cccACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTa                                                                                                        <  2:3624560/75‑1 (MQ=255)

                                                                                                                            caccaTGTAGAAACGGGCATCACACACAGATGCCC‑TATTTTTT‑GAAACTCTCGGGGTGTAGCGCAGTCCTGTAGAGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGtt                           <  2:4989731/150‑1 (MQ=18)

                                                                                                                             accaTGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTg                          >  1:4828261/1‑150 (MQ=255)

                                                                                                                             accaTGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTGTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTg                          >  2:5961626/1‑150 (MQ=255)

                                                                                                                               ttcaaaaaaaaGGGCATCTCTTTGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGt                        >  2:7794784/8‑150 (MQ=18)

                                                                                                                               caTGTAGAAAATGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTAGGGGTGTAGCGCAGTCCGGTAGCGCGCCTTCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGATCAGATGGt                        >  2:2102725/1‑150 (MQ=21)

                                                                                                                                      aaaaGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAg                 >  1:6379551/1‑150 (MQ=255)

                                                                                                                                        aaGGGCATCTCAACGAGACGCCC‑AATTTTTT‑GATTCCCTCGGCGTGTCGCGCAGTCAGGTTGCCCGCCTGCTCCGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGtt               <  2:708486/150‑1 (MQ=17)

                                                                                                                                         aggggctctcTATGAGATGCGC‑TTTTTTTTAGTAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGtt               <  1:5530535/144‑1 (MQ=14)

                                                                                                                                         aGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTg              >  2:4049457/1‑150 (MQ=255)

                                                                                                                                          gggCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTgg             >  1:5406643/1‑150 (MQ=255)

                                                                                                                                             cATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCtt          <  2:4828261/150‑1 (MQ=255)

                                                                                                                                             cATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCtt          >  1:8928909/1‑150 (MQ=255)

                                                                                                                                                ctcAACGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTa       <  1:7827860/150‑1 (MQ=255)

                                                                                                                                                  cAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAAc     <  1:2360099/150‑1 (MQ=255)

                                                                                                                                                   aaaGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAAcc    >  1:5291933/1‑150 (MQ=255)

                                                                                                                                                   aaaGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAAcc    >  1:6149953/1‑150 (MQ=255)

                                                                                                                                                   aaaGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAAcc    >  2:5371621/1‑150 (MQ=255)

                                                                                                                                                   aaaGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTACCGCAGACCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGATGGCTTTTAAca    >  2:7955261/1‑149 (MQ=255)

GCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTT‑GAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAA  >  NC_002947/733501‑733799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`