BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	827,248	C→G	6.6%	L146V (CTG→GTG)	ycaC‑I →	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	827,248	0	C	G	6.6%	1018.7 / 43.7	304	L146V (CTG→GTG)	ycaC‑I	putative hydrolase
Reads supporting (aligned to +/- strand): ref base C (130/154); new base G (9/11); total (139/165)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 305 total aligned reads displayed.

TCGTCAAAGCGGTAAAAGCCACCGGCAAGAAGCAGCTGCTGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCG  >  NC_002947/827099‑827396

tCGTCAAAGCGGTAAAAGCCACCGGCAAGAAGCAGCTGCTGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAAc                                                                                                                                                      <  2:2550946/150‑1 (MQ=255)

  gTCAAAGCGGTAAAAGCCACCGGCAAGAAGCAGCTGCTGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGACCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTg                                                                                                                                                    >  1:4575296/1‑150 (MQ=255)

    cAAAGCGGTAAAAGCCACCGGCAAGAAGCAGCTGCTGATTGCTGGTGTGGTCACTGAACTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGAc                                                                                                                                                  <  2:6074524/150‑1 (MQ=255)

      aaGCGGTAAAAGCCACCGGCAAGAAGCAGCTGCTGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGAccc                                                                                                                                                >  1:5290308/1‑150 (MQ=255)

       aGCGGTAAAAGCCACCGGCAAGAAGTAGCTGCTGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCg                                                                                                                                               <  1:2510492/150‑1 (MQ=255)

        gCGGTAAAAGCCACCGGCAAGAAGCAGCTGCTGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGt                                                                                                                                              <  1:1384249/150‑1 (MQ=255)

        gCGGTAAAAGCCACCGGCAAGAAGCAGCTGCTGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGt                                                                                                                                              <  2:1309689/150‑1 (MQ=255)

         cGGTAAAAGCCACCGGCAAGAAGCAGCTGCTGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTg                                                                                                                                             >  1:2235565/1‑150 (MQ=255)

            tAAAAGCCACCGGCAAGAAGCAGCTGCTGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACt                                                                                                                                          >  2:4810504/1‑150 (MQ=255)

                  ccACCGGCAAGAAGCAGCTGCTGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCt                                                                                                                                    <  1:7506292/150‑1 (MQ=255)

                  ccACCGGCAAGAAGCAGCTGCTGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCt                                                                                                                                    <  2:6776132/150‑1 (MQ=255)

                   cACCGGCAAGAAGCAGCTGCTGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTg                                                                                                                                   >  2:3834895/1‑150 (MQ=255)

                     ccGGCAAGAAGCAGCTGCTGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGc                                                                                                                                 <  1:908474/150‑1 (MQ=255)

                        gCAAGAAGCAGCTGCTGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGAc                                                                                                                              >  1:1069821/1‑150 (MQ=255)

                        gCAAGAAGCAGCTGCTGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGAc                                                                                                                              >  2:205273/1‑150 (MQ=255)

                          aagaagCAGCTGCTGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGc                                                                                                                            >  1:8344940/1‑150 (MQ=255)

                          aagaagCAGCTGCTGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGc                                                                                                                            >  1:2481102/1‑150 (MQ=255)

                             aagCAGCTGCTGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATg                                                                                                                         <  1:5819603/150‑1 (MQ=255)

                               gcagcTGCTGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGa                                                                                                                       <  1:5985348/150‑1 (MQ=255)

                               gcagcTGCTGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCACTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGa                                                                                                                       <  2:8238540/150‑1 (MQ=255)

                                cagcTGCTGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAg                                                                                                                      <  2:5460205/150‑1 (MQ=255)

                                     gctgATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGc                                                                                                                 >  2:8561190/1‑150 (MQ=255)

                                     gctgATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGc                                                                                                                 >  1:6932556/1‑150 (MQ=255)

                                        gATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCgg                                                                                                              <  1:372613/150‑1 (MQ=255)

                                         aTTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGGGGCTACCggg                                                                                                             <  2:7106226/150‑1 (MQ=255)

                                           tGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGgc                                                                                                           <  2:4674459/150‑1 (MQ=255)

                                           tGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGgc                                                                                                           <  2:8953670/150‑1 (MQ=255)

                                             cTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGgcgc                                                                                                         >  2:7713006/1‑150 (MQ=255)

                                              tGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCa                                                                                                        >  2:4138029/1‑150 (MQ=255)

                                              tGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCa                                                                                                        >  1:4877366/1‑150 (MQ=255)

                                                gtgtGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAAc                                                                                                      <  1:4045459/150‑1 (MQ=255)

                                                gtgtGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAAc                                                                                                      <  2:1539990/150‑1 (MQ=255)

                                                   tgGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACtga                                                                                                   <  1:8602746/150‑1 (MQ=255)

                                                    ggTCCCTGCAGTATGCGTGGCCTGCCGGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACtgat                                                                                                  <  2:7394680/150‑1 (MQ=255)

                                                     gTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACtgatg                                                                                                 >  2:5373759/1‑150 (MQ=255)

                                                     gTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACtgatg                                                                                                 <  1:6191250/150‑1 (MQ=255)

                                                      tCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACtgatga                                                                                                >  2:6619211/1‑150 (MQ=255)

                                                      tCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACtgatga                                                                                                <  1:8565155/150‑1 (MQ=255)

                                                       cACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGAc                                                                                               >  1:5464405/1‑150 (MQ=255)

                                                        aCTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGAcc                                                                                              >  1:2719785/1‑150 (MQ=255)

                                                          tGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGAccag                                                                                            >  2:4346383/1‑148 (MQ=255)

                                                          tGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTg                                                                                            <  1:3879674/150‑1 (MQ=255)

                                                          tGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTg                                                                                            >  2:5854406/1‑150 (MQ=255)

                                                              gTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTc                                                                                        <  2:3072066/150‑1 (MQ=255)

                                                               tATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCg                                                                                       >  2:6233631/1‑150 (MQ=255)

                                                                 tGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGc                                                                                     >  2:5899864/1‑150 (MQ=255)

                                                                 tGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGc                                                                                     <  1:6323791/150‑1 (MQ=255)

                                                                    gTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTg                                                                                  >  2:2731776/1‑150 (MQ=255)

                                                                     tGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTgg                                                                                 <  1:4501953/150‑1 (MQ=255)

                                                                        ccTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCt                                                                              <  1:5569662/150‑1 (MQ=255)

                                                                         cTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTg                                                                             >  1:5791680/1‑150 (MQ=255)

                                                                          ttCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGc                                                                            >  1:4113281/1‑150 (MQ=255)

                                                                          ttCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGc                                                                            <  2:645397/150‑1 (MQ=255)

                                                                             ccGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCgta                                                                         >  2:327887/1‑148 (MQ=255)

                                                                             ccGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGaa                                                                         >  2:2390525/1‑150 (MQ=255)

                                                                             ccGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGaa                                                                         <  1:3932593/150‑1 (MQ=255)

                                                                              cGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAAc                                                                        <  2:8872146/150‑1 (MQ=255)

                                                                                gCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTg                                                                      <  1:2077696/150‑1 (MQ=255)

                                                                                gCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTg                                                                      <  1:5178735/150‑1 (MQ=255)

                                                                                    tGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCAcc                                                                  <  2:1089949/150‑1 (MQ=255)

                                                                                     gTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCg                                                                 >  1:7609999/1‑150 (MQ=255)

                                                                                          cATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACt                                                            >  2:7470736/1‑150 (MQ=255)

                                                                                           aTTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTg                                                           <  1:6633908/150‑1 (MQ=255)

                                                                                           aTTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTg                                                           <  1:6041256/150‑1 (MQ=255)

                                                                                            ttGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTgg                                                          >  2:2344203/1‑150 (MQ=255)

                                                                                            ttGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTgg                                                          <  1:8266494/150‑1 (MQ=255)

                                                                                                aggaggGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCa                                                      <  1:7796599/150‑1 (MQ=255)

                                                                                                  gaggGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAAc                                                    >  2:2818904/1‑150 (MQ=255)

                                                                                                     ggtttCGATGTCTTTGTCGTCACAGATGCGTCGGGAACCTTCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGat                                                 >  2:879724/4‑149 (MQ=2)

                                                                                                           gAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGaa                                           >  1:5044128/1‑150 (MQ=255)

                                                                                                                 ttCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTg                                     <  1:4365452/150‑1 (MQ=255)

                                                                                                                 ttCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTg                                     <  2:5029486/150‑1 (MQ=255)

                                                                                                                 ttCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTg                                     <  2:7187787/150‑1 (MQ=255)

                                                                                                                  tcgtcgTCACCAACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTgg                                    <  2:555666/150‑1 (MQ=255)

                                                                                                                    gtcgtcACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGt                                  >  1:3309953/1‑150 (MQ=255)

                                                                                                                       gtcACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTAcc                               <  1:1720886/150‑1 (MQ=255)

                                                                                                                       gtcACAGATGCGTCGGGAACCTTCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTGGGTAcg                               <  2:8713582/150‑2 (MQ=2)

                                                                                                                       gtcACAGATGCGTCGGGAACCTTCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTGGGTAcg                               <  2:7579640/150‑2 (MQ=2)

                                                                                                                            cGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGtt                          >  2:3928347/1‑150 (MQ=255)

                                                                                                                             gACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTtc                         <  1:8029558/150‑1 (MQ=255)

                                                                                                                                gCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTcc                      >  2:5136117/1‑150 (MQ=255)

                                                                                                                                gCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTcc                      <  2:3710043/150‑1 (MQ=255)

                                                                                                                                gCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTcc                      >  2:2215754/1‑150 (MQ=255)

                                                                                                                                  ctctGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATc                                              <  1:6273047/124‑1 (MQ=255)

                                                                                                                                  ctctGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCaa                    >  1:841531/1‑150 (MQ=255)

                                                                                                                                   tctGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAAc                   >  1:71417/1‑150 (MQ=255)

                                                                                                                                   tctGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAAc                   <  2:7778662/150‑1 (MQ=255)

                                                                                                                                    ctGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAAcc                  <  1:6151346/150‑1 (MQ=255)

                                                                                                                                    ctGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAAcc                  <  1:2436816/150‑1 (MQ=255)

                                                                                                                                    ctGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAAcc                  <  2:7843154/150‑1 (MQ=255)

                                                                                                                                     tGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCa                 >  2:3508665/1‑150 (MQ=255)

                                                                                                                                         atgTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCt                             >  2:8434193/3‑134 (MQ=255)

                                                                                                                                           gTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATccc           <  1:2573212/150‑1 (MQ=255)

                                                                                                                                            ttCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCg          <  2:4900353/150‑1 (MQ=255)

                                                                                                                                             tCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTGGGTACGCTGTTTGCAAACCACATTCCgg         >  1:4679173/1‑150 (MQ=2)

                                                                                                                                              cAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTGGGTACGCTGTTTGCAAACCACATTCCGGa        <  1:8915581/150‑1 (MQ=2)

                                                                                                                                               aaCGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGAc       >  1:8766816/1‑150 (MQ=255)

                                                                                                                                                aCGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACt      >  2:4251018/1‑150 (MQ=255)

                                                                                                                                                 cGAACTGACCTGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCACCGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTa     >  2:6964934/1‑150 (MQ=255)

                                                                                                                                                 cGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTa     <  1:6973837/150‑1 (MQ=255)

TCGTCAAAGCGGTAAAAGCCACCGGCAAGAAGCAGCTGCTGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCG  >  NC_002947/827099‑827396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`