BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	827,278	G→A	6.7%	E156K (GAG→AAG) ‡	ycaC‑I →	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	827,278	0	G	A	6.7%	984.9 / 35.1	298	E156K (GAG→AAG) ‡	ycaC‑I	putative hydrolase
Reads supporting (aligned to +/- strand): ref base G (127/151); new base A (9/11); total (136/162)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 301 total aligned reads displayed.

GCAGCTGCTGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCC  >  NC_002947/827130‑827427

gcagcTGCTGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCACTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGa                                                                                                                                                      <  2:8238540/150‑1 (MQ=255)

 cagcTGCTGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAg                                                                                                                                                     <  2:5460205/150‑1 (MQ=255)

   gctgctGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGc                                                                                                                                                   >  2:7796608/1‑150 (MQ=255)

     tgctgATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCAACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTg                                                                                                                                                 >  1:555666/1‑150 (MQ=255)

      gctgATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGc                                                                                                                                                >  1:6932556/1‑150 (MQ=255)

          aTTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGGGGCTACCggg                                                                                                                                            <  2:7106226/150‑1 (MQ=255)

          aTTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCggg                                                                                                                                            <  2:177493/150‑1 (MQ=255)

            tGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGgc                                                                                                                                          <  2:8953670/150‑1 (MQ=255)

               tGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCa                                                                                                                                       >  1:4877366/1‑150 (MQ=255)

               tGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCa                                                                                                                                       >  2:509135/1‑150 (MQ=255)

               tGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCa                                                                                                                                       >  2:4138029/1‑150 (MQ=255)

                 gtgtGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAAc                                                                                                                                     <  1:4045459/150‑1 (MQ=255)

                 gtgtGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAAc                                                                                                                                     <  2:1539990/150‑1 (MQ=255)

                       tCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACtgatga                                                                                                                               <  1:3057691/150‑1 (MQ=255)

                       tCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACtgatga                                                                                                                               >  2:2847359/1‑150 (MQ=255)

                       tCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACtgatga                                                                                                                               <  1:8565227/150‑1 (MQ=255)

                        cACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGAc                                                                                                                              >  1:5464231/1‑150 (MQ=255)

                         aCTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGAcc                                                                                                                             >  1:2719785/1‑150 (MQ=255)

                           tGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTg                                                                                                                           <  1:7775101/150‑1 (MQ=255)

                           tGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTg                                                                                                                           >  1:6170379/1‑150 (MQ=255)

                           tGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTg                                                                                                                           >  2:1062572/1‑150 (MQ=255)

                            gAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTgg                                                                                                                          >  2:426111/1‑150 (MQ=255)

                              aGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGtt                                                                                                                        <  1:4623547/150‑1 (MQ=255)

                                tATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCg                                                                                                                      >  2:6233631/1‑150 (MQ=255)

                                  tGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGc                                                                                                                    >  2:5899864/1‑150 (MQ=255)

                                      tGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTgg                                                                                                                <  1:4501953/150‑1 (MQ=255)

                                          cTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTg                                                                                                            >  2:7540000/1‑150 (MQ=255)

                                           ttCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGc                                                                                                           >  1:580407/1‑150 (MQ=255)

                                           ttCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGc                                                                                                           >  1:4113281/1‑150 (MQ=255)

                                              ccGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCATCGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGaa                                                                                                        <  2:7396877/150‑1 (MQ=255)

                                              ccGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGaa                                                                                                        >  2:2390525/1‑150 (MQ=255)

                                               cGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCAGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAAc                                                                                                       <  1:6067833/150‑1 (MQ=255)

                                                 gCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTg                                                                                                     <  1:2077696/150‑1 (MQ=255)

                                                 gCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTg                                                                                                     <  1:5178735/150‑1 (MQ=255)

                                                    cTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCAc                                                                                                  <  1:6014919/150‑1 (MQ=255)

                                                       tCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGt                                                                                               <  2:4544868/150‑1 (MQ=255)

                                                         ggCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGa                                                                                             >  2:847848/1‑150 (MQ=255)

                                                          gCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGAc                                                                                            <  2:4747330/150‑1 (MQ=255)

                                                             ttGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTgg                                                                                         <  1:8266494/150‑1 (MQ=255)

                                                                   gaggGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGCTGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAAc                                                                                   <  2:7746912/150‑1 (MQ=255)

                                                                      ggCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGAc                                                                                <  2:8768241/150‑1 (MQ=255)

                                                                       gtttCGATGTCTTTGTCGTCACAGATGCGTCGGGAACCTTCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGata                                                                               >  1:8697904/3‑148 (MQ=2)

                                                                            gAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGaa                                                                          >  1:5044128/1‑150 (MQ=255)

                                                                            gAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGaa                                                                          >  1:5971891/1‑150 (MQ=255)

                                                                             aGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAg                                                                         >  2:8804892/1‑150 (MQ=255)

                                                                               gtctttgtcgtcACAGATGCGTCGGGAACCTTCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAggt                                                                       <  2:6220442/144‑2 (MQ=2)

                                                                                  ttCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTg                                                                    <  1:4365452/150‑1 (MQ=255)

                                                                                  ttCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTg                                                                    <  2:7187787/150‑1 (MQ=255)

                                                                                   ttgtcgtcACAGATGCGTCGGGAACCTTCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTgg                                                                   <  1:5675025/148‑1 (MQ=2)

                                                                                   tcgtcgTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTgg                                                                   <  1:3927414/150‑1 (MQ=255)

                                                                                   tcgtcgTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTgg                                                                   <  2:2794640/150‑1 (MQ=255)

                                                                                      tcgtcACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTa                                                                >  1:1384410/1‑150 (MQ=255)

                                                                                      tcgtcACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTa                                                                >  1:228598/1‑150 (MQ=255)

                                                                                       cgtcACAGATGCGTCGGGAACCTTCAATGAAGTGACCCGTCATACGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTGGGTAc                                                               >  1:741701/1‑150 (MQ=2)

                                                                                        gtcACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTAcc                                                              <  1:2473166/150‑1 (MQ=255)

                                                                                           aCCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTg                                                           >  2:6531890/1‑150 (MQ=255)

                                                                                             cGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGtt                                                         >  2:3928347/1‑150 (MQ=255)

                                                                                              gACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTtc                                                        <  1:3534740/150‑1 (MQ=255)

                                                                                              gACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTtc                                                        <  1:8029558/150‑1 (MQ=255)

                                                                                                 gCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTcc                                                     <  2:3710043/150‑1 (MQ=255)

                                                                                                 gCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTcc                                                     >  2:2215754/1‑150 (MQ=255)

                                                                                                 gCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTcc                                                     >  2:5136298/1‑150 (MQ=255)

                                                                                                   ctctGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCaa                                                   >  1:841288/1‑150 (MQ=255)

                                                                                                   ctctGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCaa                                                   <  2:5290308/150‑1 (MQ=255)

                                                                                                      tGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCa                                                >  2:3508665/1‑150 (MQ=255)

                                                                                                          atgTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCt                                                            <  1:8434193/132‑1 (MQ=255)

                                                                                                          aCGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATc                                            >  2:8569147/1‑150 (MQ=255)

                                                                                                            gTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATccc                                          <  1:2573212/150‑1 (MQ=255)

                                                                                                            gTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATccc                                          >  1:1088676/1‑150 (MQ=255)

                                                                                                             ttCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCg                                         >  2:3097729/1‑150 (MQ=255)

                                                                                                             ttCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCg                                         <  2:3288749/150‑1 (MQ=255)

                                                                                                              tCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTGGGTACGCTGTTTGCAAACCACATTCCgg                                        >  1:4679173/1‑150 (MQ=2)

                                                                                                              tCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTGGGTACGCTGTTTGCAAACCACATTCCgg                                        >  2:4895325/1‑150 (MQ=2)

                                                                                                              tCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTGGGTACGCTGTTTGCAAACCACATTCCgg                                        >  1:187888/1‑150 (MQ=2)

                                                                                                              tCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTGGGTACGCTGTTTGCAAACCACATTCCgg                                        >  2:2655530/1‑150 (MQ=2)

                                                                                                                aaCGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGAc                                      >  1:3810550/1‑150 (MQ=255)

                                                                                                                  cGAACTGACCTGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCACCGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTa                                    >  2:6964934/1‑150 (MQ=255)

                                                                                                                    aaCTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTAcc                                  <  2:2500613/150‑1 (MQ=255)

                                                                                                                        gACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCaa                              <  2:8926503/150‑1 (MQ=255)

                                                                                                                         aCCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAAc                             <  2:6658130/150‑1 (MQ=255)

                                                                                                                           ccGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCt                           >  1:7146370/1‑150 (MQ=255)

                                                                                                                              tGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCtgat                        <  1:5221314/150‑1 (MQ=255)

                                                                                                                              tGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCtgat                        <  2:552534/150‑1 (MQ=255)

                                                                                                                              tGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCtgat                        <  1:7023237/150‑1 (MQ=255)

                                                                                                                               gACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCtgatg                       >  2:679720/1‑150 (MQ=255)

                                                                                                                                aCTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCtgatga                      >  2:8368563/1‑150 (MQ=255)

                                                                                                                                aCTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCtgatga                      >  1:7884225/1‑150 (MQ=255)

                                                                                                                                 cTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGAc                     <  1:6320219/150‑1 (MQ=255)

                                                                                                                                  tCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGTCCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGTcc                    >  2:5816032/1‑150 (MQ=255)

                                                                                                                                   cGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCa                   <  1:636514/150‑1 (MQ=255)

                                                                                                                                   cGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCa                   <  2:1157643/150‑1 (MQ=255)

                                                                                                                                     gCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGc                 >  1:6561569/1‑150 (MQ=255)

                                                                                                                                        tGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTac              >  1:3244293/1‑150 (MQ=255)

                                                                                                                                        tGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTac              >  1:7559430/1‑150 (MQ=255)

                                                                                                                                        tGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTac              >  2:377438/1‑150 (MQ=255)

                                                                                                                                           cGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTacaac           <  1:8820256/150‑1 (MQ=255)

                                                                                                                                            gACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTacaaca          >  2:5746483/1‑150 (MQ=255)

                                                                                                                                               gCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGc       >  1:9001129/1‑150 (MQ=255)

                                                                                                                                               gCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGc       >  1:6844139/1‑150 (MQ=255)

                                                                                                                                                  tGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTgg    <  1:2362911/150‑1 (MQ=255)

GCAGCTGCTGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCC  >  NC_002947/827130‑827427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`