Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 827,280 G→T 6.7% E156D (GAG→GAT) ‡ ycaC‑I → putative hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947827,2800GT 6.7% 998.7 / 43.4 298E156D (GAG→GAT) ‡ycaC‑Iputative hydrolase
Reads supporting (aligned to +/- strand):  ref base G (129/149);  new base T (9/11);  total (138/160)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 300 total aligned reads displayed.

CAGCTGCTGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCA  >  NC_002947/827131‑827428
                                                                                                                                                     |                                                                                                                                                    
  gctgctGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGc                                                                                                                                                    >  2:7796608/1‑150 (MQ=255)
   ctgctgATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCt                                                                                                                                                   >  1:6934063/1‑150 (MQ=255)
    tgctgATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCAACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTg                                                                                                                                                  >  1:555666/1‑150 (MQ=255)
       tgATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCg                                                                                                                                               <  2:5561972/150‑1 (MQ=255)
         aTTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGGGGCTACCggg                                                                                                                                             <  2:7106226/150‑1 (MQ=255)
              tGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCa                                                                                                                                        >  1:4877366/1‑150 (MQ=255)
                gtgtGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAAc                                                                                                                                      <  1:4045459/150‑1 (MQ=255)
                   tgGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACtga                                                                                                                                   <  1:828683/150‑1 (MQ=255)
                     gTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACtgatg                                                                                                                                 <  1:6191250/150‑1 (MQ=255)
                      tCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACtgatga                                                                                                                                <  1:3057691/150‑1 (MQ=255)
                       cACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGAc                                                                                                                               >  1:5464231/1‑150 (MQ=255)
                        aCTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGAcc                                                                                                                              >  1:2719785/1‑150 (MQ=255)
                        aCTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGAcc                                                                                                                              >  2:8400623/1‑150 (MQ=255)
                          tGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGAccag                                                                                                                            >  2:4346383/1‑148 (MQ=255)
                          tGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTg                                                                                                                            <  1:7775101/150‑1 (MQ=255)
                          tGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTg                                                                                                                            >  2:8786024/1‑150 (MQ=255)
                             aGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGtt                                                                                                                         <  1:4623547/150‑1 (MQ=255)
                               tATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCg                                                                                                                       >  2:6233526/1‑150 (MQ=255)
                                 tGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGc                                                                                                                     <  2:2342030/150‑1 (MQ=255)
                                 tGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGc                                                                                                                     <  1:8526479/150‑1 (MQ=255)
                                    gTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTg                                                                                                                  <  1:8592907/150‑1 (MQ=255)
                                    gTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTg                                                                                                                  >  2:1379419/1‑150 (MQ=255)
                                     tGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTgg                                                                                                                 <  1:4501953/150‑1 (MQ=255)
                                     tGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTgg                                                                                                                 >  1:8045943/1‑150 (MQ=255)
                                      ggCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGc                                                                                                                <  2:5511808/150‑1 (MQ=255)
                                         cTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTg                                                                                                             >  2:7540000/1‑150 (MQ=255)
                                          ttCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGc                                                                                                            >  1:580407/1‑150 (MQ=255)
                                          ttCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGc                                                                                                            >  1:4113281/1‑150 (MQ=255)
                                             ccGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCATCGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGaa                                                                                                         <  2:7396877/150‑1 (MQ=255)
                                             ccGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGaa                                                                                                         <  2:8672907/150‑1 (MQ=255)
                                                gCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTg                                                                                                      <  1:2077696/150‑1 (MQ=255)
                                                gCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTg                                                                                                      <  1:5178735/150‑1 (MQ=255)
                                                   cTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCAc                                                                                                   <  2:4877366/150‑1 (MQ=255)
                                                     gTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCg                                                                                                 >  2:9067431/1‑150 (MQ=255)
                                                     gTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCg                                                                                                 >  1:7609999/1‑150 (MQ=255)
                                                           aTTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTg                                                                                           <  1:6633908/150‑1 (MQ=255)
                                                           aTTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTg                                                                                           <  1:6041256/150‑1 (MQ=255)
                                                                  gaggGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGCTGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAAc                                                                                    <  2:7746912/150‑1 (MQ=255)
                                                                  gaggGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAAc                                                                                    >  2:2818871/1‑150 (MQ=255)
                                                                  gaggGATTCGTTGTATTCGTCGTCACCGAAGCCTCTGGCACGTTCAACGACCTGACGCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCTGAACTGATGAAATGGTTCGGCGTGGCCTGAGAACTTCACCGTGACTGGCTCAAc                                                                                    <  2:3130763/150‑1 (MQ=255)
                                                                        ttCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATc                                                                              >  1:7025699/1‑150 (MQ=255)
                                                                           gAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGaa                                                                           >  1:5044128/1‑150 (MQ=255)
                                                                           gAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGaa                                                                           >  1:5971891/1‑150 (MQ=255)
                                                                           gAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGaa                                                                           >  2:8544525/1‑150 (MQ=255)
                                                                                 tttgtcgtcACAGATGCGTCGGGAACCTTCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTg                                                                     >  2:3289974/4‑150 (MQ=2)
                                                                                 tttgtcgtcACAGATGCGTCGGGAACCTTCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTg                                                                     >  2:356605/4‑150 (MQ=2)
                                                                                 ttCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTg                                                                     <  2:7187787/150‑1 (MQ=255)
                                                                                 ttCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTg                                                                     <  1:4365452/150‑1 (MQ=255)
                                                                                 ttCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTg                                                                     <  2:5029486/150‑1 (MQ=255)
                                                                                  ttgtcgtcACAGATGCGTCGGGAACCTTCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGCACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTgg                                                                    <  2:6730216/148‑1 (MQ=2)
                                                                                  ttgtcgtcACAGATGCGTCGGGAACCTTCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTgg                                                                    <  1:5675025/148‑1 (MQ=2)
                                                                                  tcgtcgTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTgg                                                                    <  1:3927414/150‑1 (MQ=255)
                                                                                  tcgtcgTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTgg                                                                    <  1:6459943/150‑1 (MQ=255)
                                                                                     tcgtcACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTa                                                                 >  2:6037330/1‑150 (MQ=255)
                                                                                     tcgtcACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTa                                                                 >  1:1384410/1‑150 (MQ=255)
                                                                                     tcgtcACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTa                                                                 >  1:228598/1‑150 (MQ=255)
                                                                                      cgtcACAGATGCGTCGGGAACCTTCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTGGGTAc                                                                <  2:8838747/150‑1 (MQ=2)
                                                                                       gtcACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTAcc                                                               <  1:2473166/150‑1 (MQ=255)
                                                                                       gtcACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTAcc                                                               <  1:91258/150‑1 (MQ=255)
                                                                                          aCCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTg                                                            >  2:6531890/1‑150 (MQ=255)
                                                                                            cGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGtt                                                          >  2:3928323/1‑150 (MQ=255)
                                                                                             gACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTtc                                                         <  2:6868432/150‑1 (MQ=255)
                                                                                             gACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTtc                                                         <  1:3534740/150‑1 (MQ=255)
                                                                                                gCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTcc                                                      <  2:2100582/150‑1 (MQ=255)
                                                                                                gCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTcc                                                      >  2:5136117/1‑150 (MQ=255)
                                                                                                gCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCAGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTcc                                                      >  2:5870025/1‑150 (MQ=255)
                                                                                                 ccTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCa                                                     <  1:8864237/150‑1 (MQ=255)
                                                                                                  ctctGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCaa                                                    <  2:4029641/150‑1 (MQ=255)
                                                                                                  ctctGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCaa                                                    >  1:841306/1‑150 (MQ=255)
                                                                                                    ctGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAAcc                                                  <  1:6151342/150‑1 (MQ=255)
                                                                                                     tGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCa                                                 >  2:8324830/1‑150 (MQ=255)
                                                                                                     tGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCa                                                 >  2:5417642/1‑150 (MQ=255)
                                                                                                      ggCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACtgat                                                                                                                                  <  2:3772210/68‑1 (MQ=255)
                                                                                                           gTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATccc                                           >  1:1088676/1‑150 (MQ=255)
                                                                                                           gTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATccc                                           <  1:2573212/150‑1 (MQ=255)
                                                                                                             tCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTGGGTACGCTGTTTGCAAACCACATTCCgg                                         >  1:4679173/1‑150 (MQ=2)
                                                                                                             tCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTGGGTACGCTGTTTGCAAACCACATTCCgg                                         >  1:187888/1‑150 (MQ=2)
                                                                                                              cAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGa                                        <  2:2996782/150‑1 (MQ=255)
                                                                                                               aaCGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGAc                                       >  1:3810550/1‑150 (MQ=255)
                                                                                                               aaCGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGAc                                       >  1:8766816/1‑150 (MQ=255)
                                                                                                               aaCGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGAc                                       >  2:8945772/1‑150 (MQ=255)
                                                                                                                aCGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACt                                      >  2:4251018/1‑150 (MQ=255)
                                                                                                                  gAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTAc                                    <  2:5794973/150‑1 (MQ=255)
                                                                                                                   aaCTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTAcc                                   <  2:2500613/150‑1 (MQ=255)
                                                                                                                    aCTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCg                                  >  2:1591483/1‑150 (MQ=255)
                                                                                                                          ccGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCt                            >  1:7146419/1‑150 (MQ=255)
                                                                                                                           cGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCGCCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCtg                           >  2:2422199/1‑150 (MQ=255)
                                                                                                                             tGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATTGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCtgat                         <  2:4575296/150‑1 (MQ=255)
                                                                                                                             tGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCtgat                         <  1:5221314/150‑1 (MQ=255)
                                                                                                                                cTCGGCCTGGCGTCGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGAc                      <  1:6320293/150‑1 (MQ=255)
                                                                                                                                   ggCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAg                   <  2:1069821/150‑1 (MQ=255)
                                                                                                                                    gCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGc                  >  1:7961883/1‑150 (MQ=255)
                                                                                                                                       tGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTac               >  1:3244293/1‑150 (MQ=255)
                                                                                                                                             cGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAg         <  2:2719785/150‑1 (MQ=255)
                                                                                                                                              gCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGc        >  2:5147281/1‑150 (MQ=255)
                                                                                                                                              gCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGc        >  1:6844989/1‑150 (MQ=255)
                                                                                                                                                aTGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTg      <  1:7470736/150‑1 (MQ=255)
                                                                                                                                                 tGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTgg     <  1:2362911/150‑1 (MQ=255)
                                                                                                                                                    aGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCa  <  2:6969902/150‑1 (MQ=255)
                                                                                                                                                    aGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCa  <  2:6287340/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CAGCTGCTGATTGCTGGTGTGGTCACTGAAGTATGCGTGGCCTTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCA  >  NC_002947/827131‑827428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑