Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 827,322 C→T 7.3% C170C (TGC→TGT)  ycaC‑I → putative hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947827,3220CT 7.3% 893.0 / 37.7 274C170C (TGC→TGT) ycaC‑Iputative hydrolase
Reads supporting (aligned to +/- strand):  ref base C (115/138);  new base T (9/11);  total (124/150)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

Only 100 of 277 total aligned reads displayed.

TTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTGCACGACAGCCCCCACATCAA  >  NC_002947/827173‑827469
                                                                                                                                                     |                                                                                                                                                   
   ccGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCATCGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGaa                                                                                                                                                  <  2:7396877/150‑1 (MQ=255)
   ccGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCgta                                                                                                                                                  >  2:327887/1‑148 (MQ=255)
   ccGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGaa                                                                                                                                                  <  2:8672907/150‑1 (MQ=255)
    cGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCAGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAAc                                                                                                                                                 <  1:6067833/150‑1 (MQ=255)
       cccTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGc                                                                                                                                              >  2:7140844/1‑150 (MQ=255)
         cTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCAc                                                                                                                                            <  1:6014919/150‑1 (MQ=255)
           gTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCg                                                                                                                                          >  1:7609999/1‑150 (MQ=255)
                cATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACt                                                                                                                                     <  1:2828270/150‑1 (MQ=255)
                  ttGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTgg                                                                                                                                   >  2:2344203/1‑150 (MQ=255)
                      aggaggGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCa                                                                                                                               <  1:7796599/150‑1 (MQ=255)
                        gaggGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAAc                                                                                                                             <  1:5081242/150‑1 (MQ=255)
                        gaggGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAAc                                                                                                                             >  2:2818904/1‑150 (MQ=255)
                         aggGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACg                                                                                                                            <  1:2032763/150‑1 (MQ=255)
                              ttCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATc                                                                                                                       >  1:6702453/1‑150 (MQ=255)
                              ttCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATc                                                                                                                       >  2:5091676/1‑150 (MQ=255)
                                 gAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGaa                                                                                                                    >  1:4496451/1‑150 (MQ=255)
                                 gAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGaa                                                                                                                    >  2:8544525/1‑150 (MQ=255)
                                 gAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGaa                                                                                                                    <  1:3984872/150‑1 (MQ=255)
                                  aGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAg                                                                                                                   >  2:8804892/1‑150 (MQ=255)
                                   ggTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAgg                                                                                                                  >  1:3923459/1‑150 (MQ=255)
                                    gtctttgtcgtcACAGATGCGTCGGGAACCTTCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAggt                                                                                                                 <  2:6220442/144‑2 (MQ=2)
                                      aTTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCt                                                                                                               <  1:7225555/150‑1 (MQ=255)
                                       ttCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGATGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTg                                                                                                              >  1:4418620/1‑150 (MQ=255)
                                       ttCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTg                                                                                                              >  2:8189469/1‑150 (MQ=255)
                                        ttgtcgtcACAGATGCGTCGGGAACCTTCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTgg                                                                                                             <  2:6730346/148‑1 (MQ=2)
                                        ttgtcgtcACAGATGCGTCGGGAACCTTCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTgg                                                                                                             <  1:5675025/148‑1 (MQ=2)
                                        tcgtcgTCACCAACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTgg                                                                                                             <  2:555666/150‑1 (MQ=255)
                                         cgtcgtCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTggg                                                                                                            <  1:2215980/150‑1 (MQ=255)
                                          gtcgtcACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGt                                                                                                           >  1:3309953/1‑150 (MQ=255)
                                             gtcACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTAcc                                                                                                        <  1:2515085/150‑1 (MQ=255)
                                             gtcACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTAcc                                                                                                        <  1:2473166/150‑1 (MQ=255)
                                             gtcACAGATGCGTCGGGAACCTTCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTGGGTAcg                                                                                                        <  2:7579640/150‑2 (MQ=2)
                                                aCCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTg                                                                                                     >  2:6531890/1‑150 (MQ=255)
                                                  cGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGtt                                                                                                   >  2:3928323/1‑150 (MQ=255)
                                                   gACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTtc                                                                                                  >  1:1216082/1‑150 (MQ=255)
                                                      gCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTcc                                                                                               >  2:2215754/1‑150 (MQ=255)
                                                      gCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTcc                                                                                               <  2:2100582/150‑1 (MQ=255)
                                                      gCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCAGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTcc                                                                                               >  2:5870025/1‑150 (MQ=255)
                                                        ctctGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATc                                                                                                                       <  1:6273047/124‑1 (MQ=255)
                                                        ctctGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCaa                                                                                             >  1:841288/1‑150 (MQ=255)
                                                        ctctGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCaa                                                                                             >  1:841531/1‑150 (MQ=255)
                                                          ctGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAAcc                                                                                           <  2:4209415/150‑1 (MQ=255)
                                                          ctGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAAcc                                                                                           <  1:6151346/150‑1 (MQ=255)
                                                          ctGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAAcc                                                                                           <  2:7843154/150‑1 (MQ=255)
                                                           tGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCa                                                                                          >  2:3508665/1‑150 (MQ=255)
                                                           tGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCa                                                                                          >  1:7965268/1‑150 (MQ=255)
                                                               aCGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATc                                                                                      >  2:6911451/1‑150 (MQ=255)
                                                                  ttCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCg                                                                                   <  2:4900353/150‑1 (MQ=255)
                                                                  ttCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCg                                                                                   >  2:3097729/1‑150 (MQ=255)
                                                                   tCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTGGGTACGCTGTTTGCAAACCACATTCCgg                                                                                  >  1:4679173/1‑150 (MQ=2)
                                                                    cAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTGGGTACGCTGTTTGCAAACCACATTCCGGa                                                                                 <  1:8915581/150‑1 (MQ=2)
                                                                    cAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGa                                                                                 <  1:5529975/150‑1 (MQ=255)
                                                                     aatgAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTGGGTACGCTGTTTGCAAACCACATTCCGGat                                                                                <  1:8812378/147‑2 (MQ=2)
                                                                     aaCGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGAc                                                                                >  2:8945772/1‑150 (MQ=255)
                                                                     aaCGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGAc                                                                                >  1:3810550/1‑150 (MQ=255)
                                                                     aaCGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGAc                                                                                >  2:2452994/1‑150 (MQ=255)
                                                                                ccGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCt                                                                     >  1:7146370/1‑150 (MQ=255)
                                                                                   tGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATTGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCtgat                                                                  <  2:4575296/150‑1 (MQ=255)
                                                                                   tGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCtgat                                                                  <  1:5221314/150‑1 (MQ=255)
                                                                                   tGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCtgat                                                                  <  1:7023237/150‑1 (MQ=255)
                                                                                     aCTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCtgatga                                                                >  2:8368563/1‑150 (MQ=255)
                                                                                      cTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGAc                                                               <  1:5881400/150‑1 (MQ=255)
                                                                                        cGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCa                                                             <  2:1157643/150‑1 (MQ=255)
                                                                                        cGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCa                                                             <  1:636514/150‑1 (MQ=255)
                                                                                         ggCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAg                                                            <  2:1069821/150‑1 (MQ=255)
                                                                                               gCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTacaa                                                      <  1:3168629/150‑1 (MQ=255)
                                                                                                   cGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAg                                                  <  2:2719785/150‑1 (MQ=255)
                                                                                                   cGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAg                                                  <  2:2598287/150‑1 (MQ=255)
                                                                                                    gCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGc                                                 >  1:6844139/1‑150 (MQ=255)
                                                                                                    gCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGc                                                 >  2:5147251/1‑150 (MQ=255)
                                                                                                      aTGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTg                                               <  1:7470736/150‑1 (MQ=255)
                                                                                                      aTGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTg                                               <  2:5791680/150‑1 (MQ=255)
                                                                                                       tGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTgg                                              <  1:2362911/150‑1 (MQ=255)
                                                                                                         gAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGcc                                            <  1:4138029/150‑1 (MQ=255)
                                                                                                          aGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCa                                           <  2:6287340/150‑1 (MQ=255)
                                                                                                          aGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCa                                           <  2:6969902/150‑1 (MQ=255)
                                                                                                              tGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATa                                       >  1:2055543/1‑150 (MQ=255)
                                                                                                                ccGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGt                                     <  2:5464405/150‑1 (MQ=255)
                                                                                                                ccGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGt                                     <  2:8762027/150‑1 (MQ=255)
                                                                                                                  ggggCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGc                                   <  2:3742796/150‑1 (MQ=255)
                                                                                                                  ggggCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGc                                   <  1:1062572/150‑1 (MQ=255)
                                                                                                                         aaCTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGcc                                            <  2:5359886/134‑1 (MQ=255)
                                                                                                                         aaCTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTgg                            >  1:5016206/1‑150 (MQ=255)
                                                                                                                         aaCTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTgg                            <  1:4980759/150‑1 (MQ=255)
                                                                                                                            tgatgaCCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTggggg                         >  1:6574942/1‑150 (MQ=255)
                                                                                                                            tgatgaCCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTggggg                         <  2:8862983/150‑1 (MQ=255)
                                                                                                                            tgatgaCCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTggggg                         <  1:5399984/150‑1 (MQ=255)
                                                                                                                             gatgaCCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGc                        >  1:1476291/1‑150 (MQ=255)
                                                                                                                               tgaCCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTg                      <  2:3309953/150‑1 (MQ=255)
                                                                                                                               tgaCCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTg                      >  1:8263163/1‑150 (MQ=255)
                                                                                                                                           gCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTGCACGACAGcccc          <  1:1379419/150‑1 (MQ=255)
                                                                                                                                           gCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTGCACGACAGcccc          >  2:2554136/1‑150 (MQ=255)
                                                                                                                                             gTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTGCACGACAGCCCCca        <  1:8561190/150‑1 (MQ=255)
                                                                                                                                             gTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTGCACGACAGCCCCca        <  1:6541264/150‑1 (MQ=255)
                                                                                                                                              tGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTGCACGACAGCCCCcac       <  1:9130427/150‑1 (MQ=255)
                                                                                                                                               ggCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCGGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTGCACGACAGCCCCcaca      <  2:3973041/150‑1 (MQ=255)
                                                                                                                                                   tGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCATGCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTGGACGACAGCCCCCACATCaa  >  2:4832390/1‑150 (MQ=255)
                                                                                                                                                   tGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTGCACGACAGCCCCCACATCaa  >  2:7198061/1‑150 (MQ=255)
                                                                                                                                                   tGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTGCACGACAGCCCCCACATCaa  >  1:4538578/1‑150 (MQ=255)
                                                                                                                                                   tGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTGCACGACAGCCCCCACATCaa  >  1:7812042/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TTCCCGGCCCTGTCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTGCACGACAGCCCCCACATCAA  >  NC_002947/827173‑827469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑