Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 827,334 T→C 7.5% R174R (CGT→CGC)  ycaC‑I → putative hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947827,3340TC 7.5% 881.1 / 35.5 266R174R (CGT→CGC) ycaC‑Iputative hydrolase
Reads supporting (aligned to +/- strand):  ref base T (115/130);  new base C (9/11);  total (124/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 270 total aligned reads displayed.

TCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTGCACGACAGCCCCCACATCAATCTGCAAGCGAAGC  >  NC_002947/827185‑827483
                                                                                                                                                     |                                                                                                                                                     
tCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGt                                                                                                                                                       <  2:4544868/150‑1 (MQ=255)
   gCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGAc                                                                                                                                                    <  2:4747334/150‑1 (MQ=255)
    cATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACt                                                                                                                                                   <  1:8193826/150‑1 (MQ=255)
          aggaggGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCa                                                                                                                                             <  1:7796599/150‑1 (MQ=255)
            gaggGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGCTGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAAc                                                                                                                                           <  2:7746912/150‑1 (MQ=255)
            gaggGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAAc                                                                                                                                           >  2:2818871/1‑150 (MQ=255)
            gaggGATTCGTTGTATTCGTCGTCACCGAAGCCTCTGGCACGTTCAACGACCTGACGCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCTGAACTGATGAAATGGTTCGGCGTGGCCTGAGAACTTCACCGTGACTGGCTCAAc                                                                                                                                           <  2:3130763/150‑1 (MQ=255)
             aggGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACg                                                                                                                                          <  1:2032763/150‑1 (MQ=255)
               ggCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGAc                                                                                                                                        <  2:8768241/150‑1 (MQ=255)
                gtttCGATGTCTTTGTCGTCACAGATGCGTCGGGAACCTTCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGata                                                                                                                                       >  1:8697904/3‑148 (MQ=2)
                  ttCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATc                                                                                                                                     >  2:548276/1‑150 (MQ=255)
                  ttCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATc                                                                                                                                     >  1:6702453/1‑150 (MQ=255)
                     gAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGaa                                                                                                                                  >  2:8544525/1‑150 (MQ=255)
                     gAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGaa                                                                                                                                  >  1:5971891/1‑150 (MQ=255)
                     gAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGaa                                                                                                                                  <  1:3984872/150‑1 (MQ=255)
                     gAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGaa                                                                                                                                  >  1:4496451/1‑150 (MQ=255)
                        gtctttgtcgtcACAGATGCGTCGGGAACCTTCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAggt                                                                                                                               <  2:6220660/144‑2 (MQ=2)
                          aTTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCt                                                                                                                             <  1:7225555/150‑1 (MQ=255)
                           tttgtcgtcACAGATGCGTCGGGAACCTTCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTg                                                                                                                            >  2:3289974/4‑150 (MQ=2)
                           ttCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTg                                                                                                                            <  1:4365452/150‑1 (MQ=255)
                            ttgtcgtcACAGATGCGTCGGGAACCTTCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGCACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTgg                                                                                                                           <  2:6730216/148‑1 (MQ=2)
                              gtcgtcACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGt                                                                                                                         >  1:3309953/1‑150 (MQ=255)
                               tcgtcACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTa                                                                                                                        >  2:6037330/1‑150 (MQ=255)
                               tcgtcACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTa                                                                                                                        >  1:228598/1‑150 (MQ=255)
                                cgtcACAGATGCGTCGGGAACCTTCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTGGGTAc                                                                                                                       <  2:8838747/150‑1 (MQ=2)
                                 gtcACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTAcc                                                                                                                      <  1:91258/150‑1 (MQ=255)
                                 gtcACAGATGCGTCGGGAACCTTCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTGGGTAcg                                                                                                                      <  2:8713582/150‑2 (MQ=2)
                                    aCCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTg                                                                                                                   >  1:4069318/1‑150 (MQ=255)
                                       gACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTtc                                                                                                                <  1:8029558/150‑1 (MQ=255)
                                       gACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTtc                                                                                                                >  1:1216082/1‑150 (MQ=255)
                                       gACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTtc                                                                                                                <  2:6868432/150‑1 (MQ=255)
                                       gACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTtc                                                                                                                <  1:2511255/150‑1 (MQ=255)
                                       gACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTtc                                                                                                                <  1:3534740/150‑1 (MQ=255)
                                          gCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTcc                                                                                                             >  2:5136298/1‑150 (MQ=255)
                                          gCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTcc                                                                                                             >  2:2215754/1‑150 (MQ=255)
                                          gCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTcc                                                                                                             <  2:2100582/150‑1 (MQ=255)
                                          gCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTcc                                                                                                             <  2:3710043/150‑1 (MQ=255)
                                           ccTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCa                                                                                                            <  1:5421054/150‑1 (MQ=255)
                                            ctctGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCaa                                                                                                           >  1:841531/1‑150 (MQ=255)
                                            ctctGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCaa                                                                                                           <  2:4029641/150‑1 (MQ=255)
                                              ctGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAAcc                                                                                                         <  1:2436816/150‑1 (MQ=255)
                                               tGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCa                                                                                                        >  2:5417642/1‑150 (MQ=255)
                                               tGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCa                                                                                                        >  2:8324830/1‑150 (MQ=255)
                                                   aCGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATc                                                                                                    <  1:4810504/150‑1 (MQ=255)
                                                      ttCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGACTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCg                                                                                                 >  1:5057803/1‑150 (MQ=255)
                                                       tCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTGGGTACGCTGTTTGCAAACCACATTCCgg                                                                                                >  2:2655530/1‑150 (MQ=2)
                                                       tCAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTGGGTACGCTGTTTGCAAACCACATTCCgg                                                                                                >  2:4895325/1‑150 (MQ=2)
                                                        cAATGAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTGGGTACGCTGTTTGCAAACCACATTCCGGa                                                                                               <  1:8915581/150‑1 (MQ=2)
                                                        cAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGa                                                                                               <  1:2614674/150‑1 (MQ=255)
                                                        cAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGa                                                                                               <  2:2567803/150‑1 (MQ=255)
                                                        cAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGAGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGa                                                                                               <  2:7013115/150‑1 (MQ=255)
                                                         aatgAAGTGACCCGTCATTCGGCATGGGACCGCATGAGTGCAGCCGGTGCGCAGCTGATGACCTGGTTCGGCGTTGCTTGTGAACTGCATCGCGACTGGCGCAACGATATTGAAGGTCTGGGTACGCTGTTTGCAAACCACATTCCGGat                                                                                              <  1:8812378/147‑2 (MQ=2)
                                                            gAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTAc                                                                                           <  2:5794973/150‑1 (MQ=255)
                                                            gAACTGACCCGTGACTCGGACTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTAc                                                                                           >  1:518893/1‑150 (MQ=255)
                                                             aaCTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTAcc                                                                                          <  2:2500613/150‑1 (MQ=255)
                                                              aCTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCg                                                                                         >  2:1591483/1‑150 (MQ=255)
                                                                 gACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCaa                                                                                      <  2:8926503/150‑1 (MQ=255)
                                                                    ccGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCt                                                                                   >  1:7146370/1‑150 (MQ=255)
                                                                     cGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCGCCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCtg                                                                                  >  2:2422199/1‑150 (MQ=255)
                                                                       tGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCtgat                                                                                <  1:7023237/150‑1 (MQ=255)
                                                                       tGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCtgat                                                                                <  1:4669026/150‑1 (MQ=255)
                                                                         aCTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCtgatga                                                                              >  1:7884225/1‑150 (MQ=255)
                                                                         aCTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCtgatga                                                                              >  2:8368563/1‑150 (MQ=255)
                                                                          cTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGAc                                                                             <  1:6320219/150‑1 (MQ=255)
                                                                           tCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGAcc                                                                            >  1:5657858/1‑150 (MQ=255)
                                                                           tCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGAcc                                                                            <  1:5373759/150‑1 (MQ=255)
                                                                            cGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCa                                                                           <  1:636514/150‑1 (MQ=255)
                                                                             ggCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAg                                                                          <  2:1069821/150‑1 (MQ=255)
                                                                                 tGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTac                                                                      >  2:377438/1‑150 (MQ=255)
                                                                                     gACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTacaaca                                                                  >  2:5746483/1‑150 (MQ=255)
                                                                                        gCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGc                                                               >  1:6844139/1‑150 (MQ=255)
                                                                                        gCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGc                                                               >  2:5147281/1‑150 (MQ=255)
                                                                                         cATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCt                                                              >  1:6055210/1‑150 (MQ=255)
                                                                                          aTGGAGGCTGCGGGGGCGCATCTGATGACCTTGTTCGGCGTGGCCTTCGAACTGCACCGTGACTGGCGCTACGTCATCGAATGGCTGGGTACCCTTTTCTCCAACCATATCGCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTg                                                             <  2:3488595/150‑1 (MQ=21)
                                                                                          aTGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTg                                                             <  1:7470736/150‑1 (MQ=255)
                                                                                             gAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGcc                                                          >  1:3229922/1‑150 (MQ=255)
                                                                                              aGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCa                                                         <  2:6287340/150‑1 (MQ=255)
                                                                                                 cTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAAt                                                      <  1:3834895/150‑1 (MQ=255)
                                                                                                  tGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATa                                                     <  1:2070970/150‑1 (MQ=255)
                                                                                                      ggggCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGc                                                 <  1:1062572/150‑1 (MQ=255)
                                                                                                      ggggCGCAACTGATGACCCGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGc                                                 <  1:6619211/150‑1 (MQ=255)
                                                                                                             aaCTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTgg                                          >  1:5016206/1‑150 (MQ=255)
                                                                                                             aaCTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTgg                                          <  1:2847359/150‑1 (MQ=255)
                                                                                                                 gatgaCCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGc                                      >  1:1476291/1‑150 (MQ=255)
                                                                                                                     aCCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTGCa                                  <  2:6932556/150‑1 (MQ=255)
                                                                                                                        tGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTGCACGa                               <  1:6233526/150‑1 (MQ=255)
                                                                                                                          gTTCGGCGTGACCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCGACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTGCACGACa                             <  2:2909263/150‑1 (MQ=255)
                                                                                                                           ttCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTGCACGACAg                            >  2:3938346/1‑150 (MQ=255)
                                                                                                                            tCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTGCACGACAGc                           <  2:8045943/150‑1 (MQ=255)
                                                                                                                              ggCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTGCACGACAGccc                         <  1:8324830/150‑1 (MQ=255)
                                                                                                                               gCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTGCACGACAGcccc                        <  1:1379419/150‑1 (MQ=255)
                                                                                                                               gCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTGCACGACAGcccc                        <  1:7713006/150‑1 (MQ=255)
                                                                                                                               gCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTAGCCAAATAGTGCCTTCTGGGGGCTGCACGACAGcccc                        >  2:7476799/1‑150 (MQ=255)
                                                                                                                                 gTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTGCACGACAGCCCCca                      <  1:6541264/150‑1 (MQ=255)
                                                                                                                                 gTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTGCACGACAGCCCCca                      <  1:8561190/150‑1 (MQ=255)
                                                                                                                                       tGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTGCACGACAGCCCCCACATCaa                >  2:7198061/1‑150 (MQ=255)
                                                                                                                                       tGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTGCACGACAGCCCCCACATCaa                >  1:4538578/1‑150 (MQ=255)
                                                                                                                                            aCTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTGCACGACAGCCCCCACATCAATCTGc           <  1:1876366/150‑1 (MQ=255)
                                                                                                                                              tGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTGCACGACAGCCCCCACATCAATCTGCaa         <  2:5044128/150‑1 (MQ=255)
                                                                                                                                                     tGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTGCACGACAGCCCCCACATCAATCTGCaagcgaagc  <  1:5854406/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCGGCATTGGAGGAGGGCTTCGAGGTATTCGTCGTCACCGACGCCTCTGGCACGTTCAACGAACTGACCCGTGACTCGGCCTGGCGACGCATGGAGGCTGCCGGGGCGCAACTGATGACCTGGTTCGGCGTGGCCTGCGAACTGCACCGTGACTGGCGCAACGACATCGAAGGGCTGGGTACCCTGTTCTCCAACCATATCCCGGACTACCGCAACCTGATGACCAGCTACAACAAGCTGGCCAAATAGTGCCTTCTGGGGGCTGCACGACAGCCCCCACATCAATCTGCAAGCGAAGC  >  NC_002947/827185‑827483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑